Translocatable voltage-gated Ca2+ channel β subunits in α1-β complexes reveal competitive replacement yet no spontaneous dissociation.

abstract：:Schaftoside and isoschaftoside are bioactive natural products widely distributed in higher plants including cereal crops and medicinal herbs. Their biosynthesis may be related with plant defense. However, little is known on the glycosylation biosynthetic pathway of these flavonoid di-C-glycosides with different sugar ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang ZL,Gao HM,Wang S,Zhang M,Chen K,Zhang YQ,Wang HD,Han BY,Xu LL,Song TQ,Yun CH,Qiao X,Ye M

更新日期：2020-12-01 00:00:00

abstract：:The peroxidation of polyunsaturated fatty acids leads to numerous products, including 4-hydroxynonenal (HNE). That 4-hydroxy-2-alkenal compounds react with sulfhydryl groups of proteins to form thioether adducts possessing a carbonyl function has been established [Schauenstein, E. & Esterbauer, H. (1979) Ciba Found. S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uchida K,Stadtman ER

更新日期：1992-06-15 00:00:00

abstract：:Mutations in the iPLA2-VIA/PLA2G6 gene are responsible for PARK14-linked Parkinson's disease (PD) with α-synucleinopathy. However, it is unclear how iPLA2-VIA mutations lead to α-synuclein (α-Syn) aggregation and dopaminergic (DA) neurodegeneration. Here, we report that iPLA2-VIA-deficient Drosophila exhibits defects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mori A,Hatano T,Inoshita T,Shiba-Fukushima K,Koinuma T,Meng H,Kubo SI,Spratt S,Cui C,Yamashita C,Miki Y,Yamamoto K,Hirabayashi T,Murakami M,Takahashi Y,Shindou H,Nonaka T,Hasegawa M,Okuzumi A,Imai Y,Hattori N

更新日期：2019-10-08 00:00:00

abstract：:Recognition of a particular individual occurs when we reactivate links between current perceptual inputs and the previously formed representation of that person. This recognition can be achieved by identifying, separately or simultaneously, distinct elements such as the face, silhouette, or voice as belonging to one i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sliwa J,Duhamel JR,Pascalis O,Wirth S

更新日期：2011-01-25 00:00:00

abstract：:Following carcinogen exposure in vitro, normal rat tracheal epithelial cells are transformed in a multistage process in which the cultured cells become immortal and, ultimately, neoplastic. Five cell lines derived from tumors produced by neoplastically transformed rat tracheal epithelial cells were examined for the ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walker C,Nettesheim P,Barrett JC,Gilmer TM

更新日期：1987-04-01 00:00:00

abstract：:A polypeptide was isolated from acid extracts of porcine hypothalami on the basis of its high ability to stimulate the release of corticotropin from superfused rat pituitary cells. After an initial separation by gel filtration on Sephadex G-25, further purification was carried out by reversed-phase HPLC. The isolated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patthy M,Horvath J,Mason-Garcia M,Szoke B,Schlesinger DH,Schally AV

更新日期：1985-12-01 00:00:00

abstract：:Sympatric speciation has been controversial since it was first proposed as a mode of speciation. Subterranean blind mole rats (Spalacidae) are considered to speciate allopatrically or peripatrically. Here, we report a possible incipient sympatric adaptive ecological speciation in Spalax galili (2n = 52). The study mic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hadid Y,Tzur S,Pavlícek T,Šumbera R,Šklíba J,Lövy M,Fragman-Sapir O,Beiles A,Arieli R,Raz S,Nevo E

更新日期：2013-02-12 00:00:00

abstract：:Vibrio cholerae serogroup O139 emerged on the Indian subcontinent in October 1992 to become the first non-O1 V. cholerae serogroup documented to cause epidemic cholera. Although related to V. cholerae El Tor O1 strains, O139 strains have unique surface structures that include a capsular surface layer and lipopolysacch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waldor MK,Colwell R,Mekalanos JJ

更新日期：1994-11-22 00:00:00

abstract：:A metabolic mechanism for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris has been proposed on the basis of biochemical analyses of glucose metabolism. There was a strong correlation between glucose consumption and oxalate production. Oxalic acid was found to accumulate in the culture f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Munir E,Yoon JJ,Tokimatsu T,Hattori T,Shimada M

更新日期：2001-09-25 00:00:00

abstract：:An excised patch of embryonic muscle membrane was used as a probe for measuring the release of acetylcholine (AcCho) from growing spinal neurons in Xenopus cell culture. The neuron was stimulated extracellularly at the soma, and the evoked AcCho release was monitored at the growth cone, along the neurite, and near the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun YA,Poo MM

更新日期：1987-04-01 00:00:00

abstract：:The role of global climate change in the decline of biodiversity and the emergence of infectious diseases remains controversial, and the effect of climatic variability, in particular, has largely been ignored. For instance, it was recently revealed that the proposed link between climate change and widespread amphibian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rohr JR,Raffel TR

更新日期：2010-05-04 00:00:00

abstract：:HIV-1 envelope glycoprotein is the primary target for HIV-1-specific antibodies. The native HIV-1 envelope spike on the virion surface is a trimer, but trimeric gp140 and monomeric gp120 currently are believed to induce comparable immune responses. Indeed, most studies on the immunogenicity of HIV-1 envelope oligomers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovacs JM,Nkolola JP,Peng H,Cheung A,Perry J,Miller CA,Seaman MS,Barouch DH,Chen B

更新日期：2012-07-24 00:00:00

abstract：:Using brain transcriptomic profiles from 853 individual honey bees exhibiting 48 distinct behavioral phenotypes in naturalistic contexts, we report that behavior-specific neurogenomic states can be inferred from the coordinated action of transcription factors (TFs) and their predicted target genes. Unsupervised hierar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chandrasekaran S,Ament SA,Eddy JA,Rodriguez-Zas SL,Schatz BR,Price ND,Robinson GE

更新日期：2011-11-01 00:00:00

abstract：:We previously purified a 48-kDa protein (p48) that specifically reacts with an antiserum directed against the 12 carboxyl-terminal amino acids of the c-myc gene product. Using an antiserum directed against the purified p48, we have cloned a cDNA from a human expression library. This cDNA hybrid-selects an mRNA that tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giallongo A,Feo S,Moore R,Croce CM,Showe LC

更新日期：1986-09-01 00:00:00

abstract：:The avian neuroretina (NR) is part of the central nervous system and is composed of photoreceptors, neuronal cells, and Müller (glial) cells. These cells are derived from proliferating neuroectodermal precursors that differentiate after terminal mitosis and become organized in cell strata. Genes that are specifically ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guermah M,Crisanti P,Laugier D,Dezelee P,Bidou L,Pessac B,Calothy G

更新日期：1991-05-15 00:00:00

abstract：:Malignant spreading of cancer cells requires cell surface proteases that cleave the crosslinked collagenous matrix of connective tissues. From correlating the morphologically defined invasiveness of tumor cells with the presence of specific membrane-associated proteases, we have identified a malignant human melanoma c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aoyama A,Chen WT

更新日期：1990-11-01 00:00:00

abstract：:Intracolonic administration of trinitrobenzene sulfonic acid in mice causes inflammation in the colon that is accompanied by increased expression of proinflammatory cytokines and of the substance P (SP), neurokinin 1 receptor (NK-1R) in the proximal mesenteric fat depot. We also investigated whether human mesenteric p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karagiannides I,Kokkotou E,Tansky M,Tchkonia T,Giorgadze N,O'Brien M,Leeman SE,Kirkland JL,Pothoulakis C

更新日期：2006-03-28 00:00:00

abstract：:Low-barrier hydrogen bonds (LBHBs) have been proposed to play roles in protein functions, including enzymatic catalysis and proton transfer. Transient formation of LBHBs is expected to stabilize specific reaction intermediates. However, based on experimental results and theoretical considerations, arguments against th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamaguchi S,Kamikubo H,Kurihara K,Kuroki R,Niimura N,Shimizu N,Yamazaki Y,Kataoka M

更新日期：2009-01-13 00:00:00

abstract：:Charcot-Marie-Tooth disease type 4C (CMT4C) is an early-onset, autosomal recessive form of demyelinating neuropathy. The clinical manifestations include progressive scoliosis, delayed age of walking, muscular atrophy, distal weakness, and reduced nerve conduction velocity. The gene mutated in CMT4C disease, SH3TC2/KIA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnaud E,Zenker J,de Preux Charles AS,Stendel C,Roos A,Médard JJ,Tricaud N,Kleine H,Luscher B,Weis J,Suter U,Senderek J,Chrast R

更新日期：2009-10-13 00:00:00

abstract：:Melanocyte stimulating hormone coupled to Sepharose effects an increase in tryosinase (EC 1.14.18.1; monophenol monoxygenase) activity of cultivated mouse melanoma cells. Synchronized cells are found to respond to melanocyte stimulating hormone only in the G2 phase of the cell cycle, although their response to cyclic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Varga JM,Dipasquale A,Pawelek J,McGuire JS,Lerner AB

更新日期：1974-05-01 00:00:00

abstract：:Control of transcription and enzyme activities are two interwoven regulatory systems essential for the function of a metabolic node. Saccharomyces cerevisiae strains differing in enzyme activities at the chorismate branch point of aromatic amino acid biosynthesis were constructed by recombinant DNA technology. Express...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krappmann S,Lipscomb WN,Braus GH

更新日期：2000-12-05 00:00:00

abstract：:Previous studies have shown that the helical RecA nucleoprotein filament formed on a circular single strand of DNA causes the progressive, directional transfer of a complementary strand from naked linear duplex DNA to the nucleoprotein filament, even when the duplex contains a sizable heterologous insertion. Since Rec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jwang B,Radding CM

更新日期：1992-08-15 00:00:00

abstract：:Cytoskeletal filaments assemble into dense parallel, antiparallel, or disordered networks, providing a complex environment for active cargo transport and positioning by molecular motors. The interplay between the network architecture and intrinsic motor properties clearly affects transport properties but remains poorl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richard M,Blanch-Mercader C,Ennomani H,Cao W,De La Cruz EM,Joanny JF,Jülicher F,Blanchoin L,Martin P

更新日期：2019-07-23 00:00:00

abstract：:Genome engineering in human pluripotent stem cells (hPSCs) holds great promise for biomedical research and regenerative medicine. Recently, an RNA-guided, DNA-cleaving interference pathway from bacteria [the type II clustered, regularly interspaced, short palindromic repeats (CRISPR)-CRISPR-associated (Cas) pathway] h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou Z,Zhang Y,Propson NE,Howden SE,Chu LF,Sontheimer EJ,Thomson JA

更新日期：2013-09-24 00:00:00

abstract：:TNF superfamily death ligands are expressed on the surface of immune cells and can trigger apoptosis in susceptible cancer cells by engaging cognate death receptors. A recombinant soluble protein comprising the ectodomain of Apo2 ligand/TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) has shown remarkable preclinic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nair PM,Flores H,Gogineni A,Marsters S,Lawrence DA,Kelley RF,Ngu H,Sagolla M,Komuves L,Bourgon R,Settleman J,Ashkenazi A

更新日期：2015-05-05 00:00:00

abstract：:Mitochondrial DNAs were prepared from maize lines with normal cytoplasm and with the T, C, S, and EP sources of male-sterile cytoplasms. Agarose gel electrophoresis of these preparations revealed a main high-molecular-weight DNA band. In addition, the S cytoplasm was characterized by the presence of two faster migrati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pring DR,Levings CS,Hu WW,Timothy DH

更新日期：1977-07-01 00:00:00

abstract：:The ability of polynucleotides or polynucleotide duplexes such as poly(I).poly(C) to induce interferon production is proposed to depend on the existence of certain stable glycosidic orientations. It appears that a slight increase in instability of 1--3 kcal/mole (1 cal = 4.184 J) in the conformational regions near 20 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DL,Miles DW,Eyring H

更新日期：1979-03-01 00:00:00

abstract：:The importance of gene gain through duplication has long been appreciated. In contrast, the importance of gene loss has only recently attracted attention. Indeed, studies in organisms ranging from plants to worms and humans suggest that duplication of some genes might be better tolerated than that of others. Here we h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Smet R,Adams KL,Vandepoele K,Van Montagu MC,Maere S,Van de Peer Y

更新日期：2013-02-19 00:00:00

abstract：:PAX5 is a well-known haploinsufficient tumor suppressor gene in human B-cell precursor acute lymphoblastic leukemia (B-ALL) and is involved in various chromosomal translocations that fuse a part of PAX5 with other partners. However, the role of PAX5 fusion proteins in B-ALL initiation and transformation is ill-known. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamrog L,Chemin G,Fregona V,Coster L,Pasquet M,Oudinet C,Rouquié N,Prade N,Lagarde S,Cresson C,Hébrard S,Nguyen Huu NS,Bousquet M,Quelen C,Brousset P,Mancini SJC,Delabesse E,Khamlichi AA,Gerby B,Broccardo C

更新日期：2018-10-09 00:00:00

abstract：:The intracellular signaling mechanisms regulating the generation and long-term persistence of memory T cells in vivo remain unclear. In this study, we used mouse models with conditional deletion of the key T cell receptor (TCR)-coupled adaptor molecule SH2-domain-containing phosphoprotein of 76 kDa (SLP-76), to analyz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bushar ND,Corbo E,Schmidt M,Maltzman JS,Farber DL

更新日期：2010-01-12 00:00:00