Abstract:
:This paper identifies all statistically significant dyad symmetry combinations in the Epstein-Barr virus genome. The distribution of long dyad symmetry pairings emphasizes two regions, the 5' third of the 3.1-kilobase-pair (kbp) repeat and the oriP region, the latter essential for Epstein-Barr virus replication during latency. A 600-base-pair (bp) stretch in the 3.1-kbp repeat can establish an extended hairpin loop of stem length in excess of 208 bp of predominantly G + C stacking. Moreover, the 3.1-kbp repeat has the potential to form a wide variety of secondary structures based on juxtapositions of sizable palindromes, close dyad symmetry pairings, and direct repeats. The 3.1-kbp repeat presents several features that portend it as an important control region. The oriP region contains an abundance of statistically significant dyad symmetry combinations that strongly correlate with the "21 X 30 bp" tandem repeat units and four truncated copies of this repeat unit 1 kbp downstream. Each of the units centers on the same approximately 30-bp palindrome. Contrasts in the content and the secondary structure formations associated with the 3.1-kbp repeat units versus those of the oriP region are discussed in relation to viral or cellular function.
journal_name
Proc Natl Acad Sci U S Aauthors
Karlin Sdoi
10.1073/pnas.83.18.6915subject
Has Abstractpub_date
1986-09-01 00:00:00pages
6915-9issue
18eissn
0027-8424issn
1091-6490journal_volume
83pub_type
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doi:10.1073/pnas.88.17.7792
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更新日期:1984-02-01 00:00:00
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更新日期:1989-01-01 00:00:00
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更新日期:1990-09-01 00:00:00