Abstract:
:Synchrotron radiation microtomography (SRμCT) is a nominally non-destructive 3D imaging technique which can visualise the internal structures of whole soft tissues. As a multi-stage technique, the cumulative benefits of optimising sample preparation, scanning parameters and signal processing can improve SRμCT imaging efficiency, image quality, accuracy and ultimately, data utility. By evaluating different sample preparations (embedding media, tissue stains), imaging (projection number, propagation distance) and reconstruction (artefact correction, phase retrieval) parameters, a novel methodology (combining reversible iodine stain, wax embedding and inline phase contrast) was optimised for fast (~12 minutes), high-resolution (3.2-4.8 μm diameter capillaries resolved) imaging of the full diameter of a 3.5 mm length of rat spinal cord. White-grey matter macro-features and micro-features such as motoneurons and capillary-level vasculature could then be completely segmented from the imaged volume for analysis through the shallow machine learning SuRVoS Workbench. Imaged spinal cord tissue was preserved for subsequent histology, establishing a complementary SRμCT methodology that can be applied to study spinal cord pathologies or other nervous system tissues such as ganglia, nerves and brain. Further, our 'single-scan iterative downsampling' approach and side-by-side comparisons of mounting options, sample stains and phase contrast parameters should inform efficient, effective future soft tissue SRμCT experiment design.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Strotton MC,Bodey AJ,Wanelik K,Darrow MC,Medina E,Hobbs C,Rau C,Bradbury EJdoi
10.1038/s41598-018-30520-8subject
Has Abstractpub_date
2018-08-13 00:00:00pages
12017issue
1issn
2045-2322pii
10.1038/s41598-018-30520-8journal_volume
8pub_type
杂志文章abstract::Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subject...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48364-1
更新日期:2019-08-14 00:00:00
abstract::A main controversy in cancer research is whether metastatic abilities are present in the most advanced clone of the primary tumor or result from independently acquired aberrations in early disseminated cancer cells as suggested by the linear and the parallel progression models, respectively. The genetic concordance be...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43813
更新日期:2017-03-09 00:00:00
abstract::Transition metal cobalt (Co) nanoparticle was designed as catalyst to promote the conversion reaction of Sn to SnO2 during the delithiation process which is deemed as an irreversible reaction. The designed nanocomposite, named as SnO2/Co3O4/reduced-graphene-oxide (rGO), was synthesized by a simple two-step method comp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09164
更新日期:2015-03-17 00:00:00
abstract::Advancements in microfabrication has enabled manufacturing of microscopic neurostimulation electrodes with smaller footprint than ever possible. The smaller electrodes can potentially reduce tissue damage and allow better spatial resolution for neural stimulation. Although electrodes of any shape can easily be fabrica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22545-w
更新日期:2018-03-12 00:00:00
abstract::Human milk delivers an array of bioactive components that safeguard infant growth and development and maintain healthy gut microbiota. Milk fat globule membrane (MFGM) is a biologically functional fraction of milk increasingly linked to beneficial outcomes in infants through protection from pathogens, modulation of th...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-019-47953-4
更新日期:2019-08-12 00:00:00
abstract::In solid state science, multifunctional materials and especially multiferroics have attracted a great deal of attention, as they open the possibility for next generation spintronic and data storage devices. Interestingly, while many of them host coexisting 3d and 4f elements, the role of the coupling between these two...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15150-w
更新日期:2017-11-06 00:00:00
abstract::The combined effect of drought and heat waves on insect-plant interactions is complex and not fully understood. Insects may indirectly benefit from water-deficit stress through increased plant nitrogen levels. Heat stress may have a direct negative effect, yet insect performance may be improved when day-time heat is f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37493-8
更新日期:2019-03-06 00:00:00
abstract::Porcine circovirus 2 (PCV-2) is a virus characterized by a high evolutionary rate, promoting the potential emergence of different genotypes and strains. Despite the likely relevance in the emergence of new PCV-2 variants, the subtle evolutionary patterns of PCV-2 at the individual-host level or over short transmission...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33849-2
更新日期:2018-10-19 00:00:00
abstract::Coupled networks are extremely fragile because a node failure of a network would trigger a cascade of failures on the entire system. Existing studies mainly focused on the cascading failures and the robustness of coupled networks when the networks suffer from attacks. In reality, it is necessary to recover the damaged...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08439
更新日期:2015-02-13 00:00:00
abstract::New insect pest control agents are needed to meet the demands to feed an expanding global population, to address the desire for more environmentally-friendly insecticide tools, and to fill the loss of control options in some crop-pest complexes due to development of insecticide resistance. The spinosyns are a highly e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22894-6
更新日期:2018-03-20 00:00:00
abstract::Quantification of stillbirth risk has potential to support clinical decision-making. Studies that have attempted to quantify stillbirth risk have been hampered by small event rates, a limited range of predictors that typically exclude obstetric history, lack of validation, and restriction to a single classifier (logis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62210-9
更新日期:2020-03-24 00:00:00
abstract::Oxide heterointerfaces are ideal for investigating strong correlation effects to electron transport, relevant for oxide-electronics. Using hot-electrons, we probe electron transport perpendicular to the La₀.₇Sr₀.₃MnO₃ (LSMO)- Nb-doped SrTiO₃ (Nb:STO) interface and find the characteristic hot-electron attenuation lengt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01274
更新日期:2013-01-01 00:00:00
abstract::Responses of ecosystems to human-induced stress vary in space and time, because both stressors and ecosystem vulnerabilities vary in space and time. Presently, ecosystem impact assessments mainly take into account variation in stressors, without considering variation in ecosystem vulnerability. We developed a method t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09573-8
更新日期:2017-08-24 00:00:00
abstract::Giant unilamellar vesicles (GUVs) are increasingly used as a versatile research tool to investigate membrane structure, morphology and phase state. In these studies, GUV preparation is typically enhanced by an externally applied electric field, a process called electroformation. We find that upon osmotic deflation, GU...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30286-z
更新日期:2018-08-07 00:00:00
abstract::We have studied the intrinsic fluorescence spectra of a monomeric variant of human transthyretin (M-TTR), a protein involved in the transport of the thyroid hormone and retinol and associated with various forms of amyloidosis, extending our analysis to the second order derivative of the spectra. This procedure allowed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47230-4
更新日期:2019-07-29 00:00:00
abstract::Higher and lower levels of alertness typically lead to a leftward and rightward bias in attention, respectively. This relationship between alertness and spatial attention potentially has major implications for health and safety. The current study examined alertness and spatial attention under simulated shiftworking co...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-020-71800-6
更新日期:2020-09-11 00:00:00
abstract::Transplanted neural stem cells (NSC) interact with the host brain microenvironment. A neovascularization is commonly observed in the vicinity of the cell deposit, which is correlated with behavioral improvements. To elucidate the signaling mechanisms between human NSCs and endothelial cells (ECs), these were coculture...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29029
更新日期:2016-07-04 00:00:00
abstract::Hippocampal volumes are smaller in psychiatric disorder patients and lower levels of hippocampal neurogenesis are the hypothesized cause. Understanding which molecular processes regulate hippocampal progenitor differentiation might aid in the identification of novel drug targets that can promote larger hippocampal vol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15042-z
更新日期:2017-11-08 00:00:00
abstract::Bending light in a controllable way is desired in various applications such as beam steering, navigating and cloaking. Different from the conventional way to bend light by refractive index gradient, transformation optics or special beams through wavefront design such as Airy beams and surface plasmons, we proposed a m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15805
更新日期:2015-10-29 00:00:00
abstract::Oestrogen is an important regulator in reproduction. To understand the role of oestrogen receptor 1 (ESR1) in Leydig cells, we investigated the expression of ESR1 in mouse Leydig cells during postnatal development and the effects of oestrogen on steroidogenesis and proliferation of progenitor Leydig cells (PLCs). In L...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43459
更新日期:2017-03-07 00:00:00
abstract::Rapid diagnostic tests (RDTs) have been developed to detect influenza A virus for the swift diagnosis and management of patients. However, despite the simplicity and convenience, the low sensitivity of RDTs remains a limitation for their use in point of care testing (POCT). In this study, we developed a rapid fluoresc...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-018-31786-8
更新日期:2018-09-07 00:00:00
abstract::Studies focusing on biofilm assembly in deep-sea environments are rarely conducted. To examine the effects of substrate type on microbial community assembly, biofilms were developed on different substrates for different durations at two locations in the Red Sea: in a brine pool and in nearby bottom water (NBW) adjacen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06647
更新日期:2014-10-17 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-61343-1
更新日期:2020-03-04 00:00:00
abstract::Selective serotonin reuptake inhibitors are among the most prescribed antidepressants. Fluoxetine is the lead molecule which exerts its therapeutic effects, at least in part, by promoting neuroplasticity through increased brain-derived neurotrophic factor (BDNF)/tropomyosin-related receptor kinase B (TrkB) signalling....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42775-w
更新日期:2019-04-19 00:00:00
abstract::Compromised activity of the DNA repair enzymes may raise the risk of a number of cancers. We analyzed polymorphisms in the Xeroderma Pigmentosum, Complementation Group C (XPC) gene for their correlation with urinary bladder cancer. Ala499Val and Lys939Gln polymorphisms were genotyped in 234 urinary bladder cancer case...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/srep27018
更新日期:2016-06-01 00:00:00
abstract::Ocean warming (OW) and acidification (OA) are key features of global change and are predicted to have negative consequences for marine species and ecosystems. At a smaller scale increasing oil and gas activities at northern high latitudes could lead to greater risk of petroleum pollution, potentially exacerbating the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35623-w
更新日期:2018-11-26 00:00:00
abstract::Layered-structure materials are currently relevant given their quasi-2D nature. Knowledge of their physical properties is currently of major interest. Niobium ditelluride possesses a monoclinic layered-structure with a distortion in the tellurium planes. This structural complexity has hindered the determination of its...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35308-4
更新日期:2018-11-19 00:00:00
abstract::TET aminopeptidases are dodecameric particles shared in the three life domains involved in various biological processes, from carbon source provider in archaea to eye-pressure regulation in humans. Each subunit contains a dinuclear metal site (M1 and M2) responsible for the enzyme catalytic activity. However, the role...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20876
更新日期:2016-02-08 00:00:00
abstract::In higher plants, L-galactono-1,4-lactone dehydrogenase (GLDH) plays important roles in ascorbic acid (AsA) biosynthesis and assembly of respiration complex I. Here we report three homoeologous genes (TaGLDH-A1, -B1 and -D1) encoding common wheat GLDH isozymes and a unique allelic variant (TaGLDH-A1b) associated with ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30177
更新日期:2016-07-22 00:00:00
abstract::Up to 30% of all breast cancer cases may be inherited and up to 85% of those may be due to segregation of susceptibility genes with low and moderate risk [odds ratios (OR) ≤ 3] for (mostly peri- and post-menopausal) breast cancer. The majority of low/moderate-risk genes, particularly those with minor allele frequencie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77037-7
更新日期:2020-12-04 00:00:00