Abstract:
:In vitro run-off transcription by T7 RNA polymerase generates heterogeneous 3'-ends because the enzyme tends to add untemplated adenylates. To generate homogeneous 3'-termini, HDV ribozymes have been used widely. Their sequences are added to the 3'-terminus such that co-transcriptional self-cleavage generates homogeneous 3'-ends. A shorter HDV sequence that cleaves itself efficiently would be advantageous. Here we show that a recently discovered, small HDV ribozyme is a good alternative to the previously used HDV ribozyme. The new HDV ribozyme is more efficient in some sequence contexts, and less efficient in other sequence contexts than the previously used HDV ribozyme. The smaller size makes the new HDV ribozyme a good alternative for transcript 3'-end processing.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Akoopie A,Müller UFdoi
10.1007/s00239-018-9861-9subject
Has Abstractpub_date
2018-08-01 00:00:00pages
425-430issue
7eissn
0022-2844issn
1432-1432pii
10.1007/s00239-018-9861-9journal_volume
86pub_type
杂志文章abstract::Insertions and deletions of entire codons have recently been discovered as a mechanism by which B cells, in addition to conventional base substitution, evolve the antibodies produced by their immunoglobulin genes. These events frequently seem to involve repetitive sequence motifs in the antibody-encoding genes, and it...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-001-0049-2
更新日期:2002-03-01 00:00:00
abstract::RNA editing affecting chloroplast and mitochondrial genomes has been identified in all major clades of land plants. The frequency of edited sites varies greatly between lineages but hornworts represent an extreme in propensity for editing in both their chloroplast and mitochondrial genomes. cDNA sequences from seven t...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/pl00006141
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-008-9195-0
更新日期:2009-02-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00173165
更新日期:1995-12-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00163228
更新日期:1995-03-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02101791
更新日期:1990-07-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-018-9846-8
更新日期:2018-06-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 信件
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更新日期:2020-08-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-015-9670-3
更新日期:2015-04-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02602909
更新日期:1989-11-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-005-0292-z
更新日期:2006-10-01 00:00:00
abstract::Variation in structure among primate hemoglobins is associated with variation in function. This supports the hypothesis that most substitutions observed among homologous proteins in different species have been fixed by natural selection because they contribute to the fitness of the genotype. It does not support the co...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF01653958
更新日期:1972-12-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s002390010120
更新日期:2000-12-01 00:00:00
abstract::EcoRI restriction of the B. nigra rDNA recombinants, isolated from a lambda genomic library, showed that the 3.9-kb fragment corresponded to the Intergenic Spacer (IGS), which was sequenced and found to be 3,928 bp in size. Sequence and dot-matrix analyses showed that the organization of the B. nigra rDNA IGS was typi...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02337518
更新日期:1996-11-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02339009
更新日期:1996-10-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-018-9870-8
更新日期:2018-12-01 00:00:00
abstract::DNA sequences representing approximately 40% of the large-subunit rRNA gene from the lower dipteran Chironomus thummi were analyzed. Once aligned with their Drosophila counterparts, sequence and base content comparisons were carried out. Sequence identity was found to be high overall, except for six regions that displ...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00173183
更新日期:1995-12-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-004-2606-y
更新日期:2004-07-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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journal_title:Journal of molecular evolution
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更新日期:2014-05-01 00:00:00
abstract::The best reconstructions of the history of life will use both molecular time estimates and fossil data. Errors in molecular rate estimation typically are unaccounted for and no attempts have been made to quantify this uncertainty comprehensively. Here, focus is primarily on fossil calibration error because this error ...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
abstract::Fish protamines are highly specialized molecules which are responsible for chromatin condensation during the last stages of spermatogenesis (spermiogenesis). However, not all fish contain protamines in their sperm nuclei; rather, there seems to be a random distribution of protamines within this group. The origin of th...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00160152
更新日期:1994-09-01 00:00:00
abstract::There has been considerable discussion in recent years on the evolution of the tandemly repeated multigene families, since some organisms show a concerted model whereas others show a birth-and-death model. This controversial subject extends to several species of fish. In this study, three species of the Sparidae famil...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-013-9541-8
更新日期:2013-03-01 00:00:00
abstract::A fundamental paradox motivates the study of plant mitochondrial genomics: the mutation rate is very low (lower than in the nucleus) but the rearrangement rate is high. A landmark paper published in Journal of Molecular Evolution in 1988 established these facts and revealed the paradox. Jeffrey Palmer and Laura Herbon...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-020-09980-y
更新日期:2021-01-24 00:00:00
abstract::Restriction modification (RM) systems serve to protect bacteria against bacteriophages. They comprise a restriction endonuclease activity that specifically cleaves DNA and a corresponding methyltransferase activity that specifically methylates the DNA, thereby protecting it from cleavage. Such systems are very common ...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02198833
更新日期:1996-02-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-002-2420-3
更新日期:2003-05-01 00:00:00