当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Organization of the Dictyostelium discoideum actin multigene family. Flanking sequences show subfamily homologies and unusual dyad symmetries.

Organization of the Dictyostelium discoideum actin multigene family. Flanking sequences show subfamily homologies and unusual dyad symmetries.

Abstract：

:Sequences flanking the protein-coding regions of 15 of the 17 to 20 actin genes in the cellular slime mold Dictyostelium discoidium have been determined. Comparison of sequences among genes shows that they contain extensive homologies at both the 5' and 3' ends of the coding regions. On the basis of these homologies, actin genes fall into three groups. Group I consists of Actin 8 alone. Group II consists of the two closely linked genes Actin 3-sub1 and Actin 3-sub2. These two genes differ from all other actin genes in the location of their TATA box and oligo(dT) run, and diverge substantially in their coding sequence as well. Group III contains all the rest of the genes we have studied. Within this group, there are two subgroups of genes, IIIA (Actins 5, 9 and 10) and IIIB (Actins M6, 2-sub1 and 2-sub2, 4, 6, 7, 11 and 12). Two actin cDNA clones, ITL-1 and III-12/A1, which have no cloned genomic counterparts, are members of groups IIIA and IIIB, respectively. Homologies at the 3' ends of genes do not extend beyond a short genomic poly(A) sequence, the probable termination of transcription. Homologies at the 5' ends may extend about 300 base-pairs 5' to the ATG but, in most cases, extend only about 150 base-pairs 5' to the ATG. We have identified a group of short, relatively G + C-rich sequences within the extremely A + T-rich sequence at the 5' ends of actin-coding regions, which are shared among different actin genes. Many of these sequences exhibit dyad symmetry, and their general location and order is conserved among the different actin genes. We suggest that they may have a role in regulation of the transcriptional patterns of individual actin genes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Romans P,Firtel RA

doi

10.1016/0022-2836(85)90003-8

subject

Has Abstract

pub_date

1985-06-05 00:00:00

pages

311-26

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(85)90003-8

journal_volume

183

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • A New Type of Circular RNA derived from Nonconventional Introns in Nuclear Genes of Euglenids.

    abstract：:Nuclear protein-coding genes of euglenids (Discoba, Euglenozoa, Euglenida) contain conventional (spliceosomal) and nonconventional introns. The latter have been found only in euglenozoans. A unique feature of nonconventional introns is the ability to form a stable and slightly conserved RNA secondary structure bringin...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.166758

    authors： Gumińska N,Zakryś B,Milanowski R

    更新日期：2021-02-05 00:00:00

  • Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1.

    abstract：:The human alternative splicing factor ASF/SF2, an SR (serine-arginine-rich) protein involved in mRNA splicing control, is activated by the multisite phosphorylation of its C-terminal RS domain, a segment containing numerous arginine-serine dipeptide repeats. The protein kinase responsible for this modification, SR-spe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.08.029

    authors： Ma CT,Velazquez-Dones A,Hagopian JC,Ghosh G,Fu XD,Adams JA

    更新日期：2008-02-08 00:00:00

  • Characterization of the heterogeneity and specificity of interpolypeptide interactions in amyloid protofibrils by measurement of site-specific fluorescence anisotropy decay kinetics.

    abstract：:The aggregation of proteins often results in highly ordered fibrillar structures. While significant insights have been obtained on structural aspects of amyloid fibrils, little is known about the structures of protofibrils, which are presumed to be the precursors of fibrils. An understanding of the molecular mechanism...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.08.053

    authors： Jha A,Udgaonkar JB,Krishnamoorthy G

    更新日期：2009-10-30 00:00:00

  • Multiple DNA conformational changes induced by an initiator protein precede the nicking reaction in a rolling circle replication origin.

    abstract：:The core origin for plus strand DNA replication of filamentous bacteriophage f1 binds the initiator protein (gpII), which subsequently introduces a specific nick in the plus strand. The core origin consists of a nicking region and a binding region. The binding of gpII occurs in two steps, forming a binding intermediat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1242

    authors： Higashitani A,Greenstein D,Hirokawa H,Asano S,Horiuchi K

    更新日期：1994-04-08 00:00:00

  • The influence of helix morphology on co-operative polyamide backbone conformational flexibility in peptide nucleic acid complexes.

    abstract：:A systematic analysis of peptide nucleic acid (PNA) complexes deposited in the Protein Data Bank has been carried out using a set of contiguous atom torsion angle definitions. The analysis is complemented by molecular mechanics adiabatic potential energy calculations on hybrid PNA-nucleic acid model systems. Hitherto ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3131

    authors： Topham CM,Smith JC

    更新日期：1999-10-08 00:00:00

  • Crystallization and preliminary X-ray analysis of gamma-glutamyltranspeptidase from Escherichia coli K-12.

    abstract：:gamma-Glutamyltranspeptidase (EC 2.3.2.2) from Escherichia coli K-12 has been purified and crystallized by means of vapor diffusion in hanging drops. Two kinds of crystals on cell dimensions were found for X-ray diffraction analysis, one from ammonium sulfate and the other from polyethylene glycol 6000 as precipitants...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1677

    authors： Kumagai H,Nohara S,Suzuki H,Hashimoto W,Yamamoto K,Sakai H,Sakabe K,Fukuyama K,Sakabe N

    更新日期：1993-12-20 00:00:00

  • Determination of the MurD mechanism through crystallographic analysis of enzyme complexes.

    abstract：:UDP -N- acetylmuramoyl- L -alanine: D -glutamate (MurD) ligase catalyses the addition of d -glutamate to the nucleotide precursor UDP -N- acetylmuramoyl- L -alanine (UMA). The crystal structures of three complexes of Escherichia coli MurD with a variety of substrates and products have been determined to high resolutio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2800

    authors： Bertrand JA,Auger G,Martin L,Fanchon E,Blanot D,Le Beller D,van Heijenoort J,Dideberg O

    更新日期：1999-06-11 00:00:00

  • Enzyme catalysis via control of activation entropy: site-directed mutagenesis of 6,7-dimethyl-8-ribityllumazine synthase.

    abstract：:6,7-Dimethyl-8-ribityllumazine synthase (lumazine synthase) catalyses the penultimate step in the biosynthesis of riboflavin. In Bacillus subtilis, 60 lumazine synthase subunits form an icosahedral capsid enclosing a homotrimeric riboflavin synthase unit. The ribH gene specifying the lumazine synthase subunit can be e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01473-0

    authors： Fischer M,Haase I,Kis K,Meining W,Ladenstein R,Cushman M,Schramek N,Huber R,Bacher A

    更新日期：2003-02-21 00:00:00

  • Residues of the cytoplasmic domain of MotA essential for torque generation in the bacterial flagellar motor.

    abstract：:The MotA protein of Escherichia coli is a component of the flagellum that functions, together with MotB, in transmembrane proton conduction. MotA and MotB are believed to form the stator of the flagellar motor. They are integral membrane proteins; MotA has a large (ca 22 kDa) domain in the cytoplasm, and MotB a much s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1316

    authors： Zhou J,Blair DF

    更新日期：1997-10-24 00:00:00

  • Molecular requirements for duplex recognition and cleavage by eukaryotic RNase III: discovery of an RNA-dependent DNA cleavage activity of yeast Rnt1p.

    abstract：:Members of the double-stranded RNA (dsRNA) specific RNase III family are known to use a conserved dsRNA-binding domain (dsRBD) to distinguish RNA A-form helices from DNA B-form ones, however, the basis of this selectivity and its effect on cleavage specificity remain unknown. Here, we directly examine the molecular re...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.02.059

    authors： Lamontagne B,Hannoush RN,Damha MJ,Abou Elela S

    更新日期：2004-04-23 00:00:00

  • Premature stop codons inactivate the RT6 genes of the human and chimpanzee species.

    abstract：:RT6 is a glycosyl phosphatidylinositol-anchored cell membrane protein, whose expression is restricted to peripheral T cells and intraepithelial lymphocytes. It has attracted interest as a T cell differentiation marker and activation antigen in rats. The only known protein to which RT6 shows significant homology is a r...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1680

    authors： Haag F,Koch-Nolte F,Kühl M,Lorenzen S,Thiele HG

    更新日期：1994-10-28 00:00:00

  • X-ray crystal structure of the rotavirus inner capsid particle at 3.8 A resolution.

    abstract：:The rotavirus inner capsid particle, known as the "double-layered particle" (DLP), is the "payload" delivered into a cell in the process of viral infection. Its inner and outer protein layers, composed of viral protein (VP) 2 and VP6, respectively, package the 11 segments of the double-stranded RNA (dsRNA) of the vira...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.01.055

    authors： McClain B,Settembre E,Temple BR,Bellamy AR,Harrison SC

    更新日期：2010-03-26 00:00:00

  • Isolation and characterization of conditional alleles of the yeast SIR2 gene.

    abstract：:Sir2 is a protein deacetylase that mediates transcriptional silencing at the HM loci, telomeres, and rDNA repeats in yeast. To identify functionally significant regions of the Sir2 protein, we have characterized two types of mutations. First, we used random mutagenesis to create temperature-sensitive alleles of the SI...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.01.044

    authors： Hickman M,McCullough K,Woike A,Raducha-Grace L,Rozario T,Dula ML,Anderson E,Margalit D,Holmes SG

    更新日期：2007-04-13 00:00:00

  • Identification of elements essential for transcription in Brugia malayi promoters.

    abstract：:Little is known concerning promoter structure in the filarial parasites. Recently, transient transfection methods have been developed for the human filarial parasite Brugia malayi. These methods have been employed to localize the promoter for the 70kDa heat shock protein (BmHSP70) to a region extending 394nt upstream ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.08.014

    authors： Higazi TB,Deoliveira A,Katholi CR,Shu L,Barchue J,Lisanby M,Unnasch TR

    更新日期：2005-10-14 00:00:00

  • Evolution of function in protein superfamilies, from a structural perspective.

    abstract：:The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as defined by the CATH protein structure classification, by way of the Enzyme Commission (EC) scheme. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4513

    authors： Todd AE,Orengo CA,Thornton JM

    更新日期：2001-04-06 00:00:00

  • C--H...O hydrogen bond involving proline residues in alpha-helices.

    abstract：:Despite proline being assumed to be a helix-breaker, a large number of alpha-helices are found to contain Pro in globular as well as membrane proteins. Proline has no free NH group and therefore cannot form the conventional intra-helical NH.O=C hydrogen bond. An analysis of known protein structures has shown that the ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2199

    authors： Chakrabarti P,Chakrabarti S

    更新日期：1998-12-11 00:00:00

  • Conformational changes in the multidrug transporter EmrE associated with substrate binding.

    abstract：:EmrE is a bacterial multidrug transporter of the small multidrug resistance family, which extrudes large hydrophobic cations such as tetraphenylphosphonium (TPP(+)) out of the cell by a proton antiport mechanism. Binding measurements were performed on purified EmrE solubilized in dodecylmaltoside to determine the stoi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00895-7

    authors： Tate CG,Ubarretxena-Belandia I,Baldwin JM

    更新日期：2003-09-05 00:00:00

  • Mapping of the docking of SecA onto the chaperone SecB by site-directed spin labeling: insight into the mechanism of ligand transfer during protein export.

    abstract：:Export of protein into the periplasm of Escherichia coli via the general secretory system is achieved by action of a ternary complex comprising the polypeptide ligand, the chaperone SecB and SecA, a peripheral component of the membrane translocon, which is itself an ATPase. The unfolded ligand is captured initially by...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.08.022

    authors： Crane JM,Mao C,Lilly AA,Smith VF,Suo Y,Hubbell WL,Randall LL

    更新日期：2005-10-21 00:00:00

  • Structure Determination of the Nuclear Pore Complex with Three-Dimensional Cryo electron Microscopy.

    abstract：:Determining the structure of the nuclear pore complex (NPC) imposes an enormous challenge due to its size, intricate composition and membrane-embedded nature. In vertebrates, about 1000 protein building blocks assemble into a 110-MDa complex that fuses the inner and outer membranes of a cell's nucleus. Here, we review...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2016.01.004

    authors： von Appen A,Beck M

    更新日期：2016-05-22 00:00:00

  • Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses.

    abstract：:A detailed phylogenetic analysis for mammalian members of the family Herpesviridae, based on molecular sequences is reported. Sets of encoded amino acid sequences were collected for eight well conserved genes that are common to mammalian herpesviruses. Phylogenetic trees were inferred from alignments of these sequence...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0152

    authors： McGeoch DJ,Cook S,Dolan A,Jamieson FE,Telford EA

    更新日期：1995-03-31 00:00:00

  • Multiple roles of prolyl residues in structure and folding.

    abstract：:To explore the ways that proline residues may influence the conformational options of a polypeptide backbone, we have characterized Pro-->Ala mutants of cellular retinoic acid-binding protein I (CRABP I). While all three Xaa-Pro bonds are in the trans conformation in the native protein and the equilibrium stability of...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4002

    authors： Eyles SJ,Gierasch LM

    更新日期：2000-08-18 00:00:00

  • Effect of single DNA lesions on in vitro replication with DNA polymerase III holoenzyme. Comparison with other polymerases.

    abstract：:In the present work, we have studied in vitro replication of N-2-acetylaminofluorene (AAF) or cis-diamminedichloroplatinum II (cis-DDP) single modified DNA templates. We used the holoenzyme (pol III HE) or the alpha subunit of DNA polymerase III, which is involved in SOS mutagenesis, and other DNA polymerases in order...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1125

    authors： Belguise-Valladier P,Maki H,Sekiguchi M,Fuchs RP

    更新日期：1994-02-11 00:00:00

  • The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions.

    abstract：:An essential step of pre-mRNA spliceosome assembly is the interaction between the snRNPs U4/U6 and U5, to form the [U4/U6.U5] tri-snRNP. While the tri-snRNP protein Prp6p appears to play an important role for tri-snRNP formation in yeast, little is known about the interactions that connect the two snRNP particles in h...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3685

    authors： Makarov EM,Makarova OV,Achsel T,Lührmann R

    更新日期：2000-05-12 00:00:00

  • Transcription from the rha operon psr promoter.

    abstract：:S1 nuclease mapping experiments performed with RNA extracted from cell lines that were unable to metabolize L-rhamnose demonstrated that L-rhamnose and not a metabolite was the inducer of the L-rhamnose operons of Escherichia coli. In vitro transcription studies showed that purified RhaR activates transcription from t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90003-5

    authors： Tobin JF,Schleif RF

    更新日期：1990-01-05 00:00:00

  • Selectional and mutational scope of peptides sequestering the Jun-Fos coiled-coil domain.

    abstract：:The activator protein-1 (AP-1) complex plays a crucial role in numerous pathways, and its ability to induce tumorigenesis is well documented. Thus, AP-1 represents an interesting therapeutic target. We selected peptides from phage display and compared their ability to disrupt the cFos/cJun interaction to a previously ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.04.030

    authors： Hagemann UB,Mason JM,Müller KM,Arndt KM

    更新日期：2008-08-01 00:00:00

  • High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds.

    abstract：:Proteinaceous serine proteinase inhibitors are widespread throughout the plant kingdom where they play an important role in protection against pests and pathogens. Here, we describe the isolation and characterisation of a novel 14 amino acid residue cyclic peptide from sunflower seeds, which is a potent inhibitor of t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2891

    authors： Luckett S,Garcia RS,Barker JJ,Konarev AV,Shewry PR,Clarke AR,Brady RL

    更新日期：1999-07-09 00:00:00

  • Linkage of protonation and anion binding to the folding of Sac7d.

    abstract：:The temperature, pH, and salt dependence of the folding of recombinant Sac7d from the hyperthermophile Sulfolobus acidocaldarius is mapped using multi-dimensional differential scanning calorimetry (DSC) and folding progress surfaces followed by circular dichroism. Linkage relations are derived to explain the observed ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1500

    authors： McCrary BS,Bedell J,Edmondson SP,Shriver JW

    更新日期：1998-02-13 00:00:00

  • Probing myosin head structure with monoclonal antibodies.

    abstract：:Monoclonal antibodies that react with defined regions of the heavy and light chains of chicken skeletal muscle myosin have been used to provide a correlation between the primary and the tertiary structures of the head. Electron microscopy of rotary shadowed antibody-myosin complexes shows that the sites for three epit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(86)80009-2

    authors： Winkelmann DA,Lowey S

    更新日期：1986-04-20 00:00:00

  • Translation elongation factor EF-Tu modulates filament formation of actin-like MreB protein in vitro.

    abstract：:EF-Tu has been shown to interact with actin-like protein MreB and to affect its localization in Escherichia coli and in Bacillus subtilis cells. We have purified YFP-MreB in an active form, which forms filaments on glass slides in vitro and was active in dynamic light-scattering assays, polymerizing in milliseconds af...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2015.01.025

    authors： Defeu Soufo HJ,Reimold C,Breddermann H,Mannherz HG,Graumann PL

    更新日期：2015-04-24 00:00:00

  • Transferrin, is a mixed chelate-protein ternary complex involved in the mechanism of iron uptake by serum-transferrin in vitro?

    abstract：:Iron uptake by transferrin from triacetohydroxamatoFe(III) (Fe(AHA)3) in the presence of bicarbonate has been investigated between pH 7 and 8.2. The protein transits from the opened apo- to the closed holoform by several steps with the accumulation of at least three kinetic intermediates. All these steps are accompani...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3238

    authors： Pakdaman R,Abdallah FB,El Hage Chahine JM

    更新日期：1999-11-12 00:00:00