Abstract：
:When deaths occur in water, soft tissue decomposes after a temperature-dependent period, making DNA identification dependent on bone. This study examined the effects of water on bone DNA purity and quantity, and determined the best of three extraction methods for isolating DNA. The organic phenol-chloroform method consistently extracted DNA approximating the accepted 260/280 purity value (~1.8); ChargeSwitch® gDNA Plant Kit and DNeasy Blood and Tissue Kit produced fair and unacceptable values, respectively. The purity value for humerus and rib samples was consistent across accumulated degree days (ADD). Significant differences in quantification among extraction methods and between bone types were identified. Ribs and ChargeSwitch® gDNA Plant Kit samples produced the lowest mean Ct values of the bone types and the extraction methods, respectively. Therefore, this study proposes that magnetic bead technology extraction methods and ribs be considered when processing bones that have been submerged in water for any length of time.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Cartozzo C,Singh B,Boone E,Simmons Tdoi
10.1111/1556-4029.13792subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1830-1835issue
6eissn
0022-1198issn
1556-4029journal_volume
63pub_type
杂志文章abstract：:The 1970s have yielded many situations in which psychiatrists have been summoned by agents of the state for help in resolving terrorist-related crises. This paper tries to examine the evolving role of forensic psychiatrists as they participate in such situations and reviews some of the functions involved in open commu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Human ecchinococcosis also known as hydatid disease is a zoonotic infection caused by the tapeworm Ecchinococcus with 2-3 Million cases worldwide. We hereby report a 6 years period study of Sudden death due to hydatidosis aiming to analyze the epidemiological criteria, death circumstances, and autopsy observations att...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12172
更新日期：2013-09-01 00:00:00
abstract：:This research tests the accuracy of age-at-death estimation from the sternal end of the fourth rib. Age was estimated using the Işcan and Loth casts and written descriptions. The correlation results indicate that there are significant differences in the observed versus actual ages (r = 0.75329, p < 0.001) and that the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01415.x
更新日期：2010-09-01 00:00:00
abstract：:In 2012, the Harris County Institute of Forensic Sciences began prospectively collecting injury data from pediatric autopsies. These data and associated case information from 635 pediatric cases are archived in the Infant Injury Database (IID). This paper introduces the IID to the forensic community and demonstrates i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14120
更新日期：2019-11-01 00:00:00
abstract：:The U.S. agricultural sector is vulnerable to intentionally introduced microbial threats because of its wide and open distribution and economic importance. To investigate such events, forensically valid assays for plant pathogen detection are needed. In this work, real-time PCR assays were developed for three model pl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12321
更新日期：2014-03-01 00:00:00
abstract：:Urea nitrogen, glucose, sodium, potassium, and chloride were measured in common vitreous humor samples using a variety of instruments. There was found to be variation in values obtained by the different procedures for each of these constituents. The variation in electrolyte values between the different procedures can ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:DNA profiling using STRs on the 310 and 3100 Genetic Analyzers routinely generates electropherograms that are analyzed with the GeneScan software available from the instrument's manufacturer, Applied Biosystems. Users have been able to choose from three different smoothing options that have been known to result in sig...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:A 48-year-old woman was found dead on a chair in her living room. She had received dialysis every day because of chronic renal failure for the past 15 years. On a table beside her, there was a mirror and 10-mL syringe on a napkin. A stopper was out of place in a portion of a three-way blood access tube established in ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01471.x
更新日期：2010-11-01 00:00:00
abstract：:A basilar skull fracture that extends along both petrous bones is generally considered to be a response to impacts to the lateral aspects of the head. This generalization is not warranted as such a fracture often results from impacts to any point around the base of the skull or to the chin. These facts were appreciate...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:The use of certain fluorescing vapor-phase chemicals for the development of latent fingerprints is described. Some advantages of these chemicals over other methods that use fluorescers are discussed. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC-4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty-one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identifica...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01356.x
更新日期：2010-05-01 00:00:00
abstract：:In the summer of 1984, police in Pinellas County, Florida, confiscated six identically colored imported Asian skulls (in a shipping case) from a private citizen. In May 1988, in nearby Hillsborough County, police confiscated a very similar skull from another private citizen, who allegedly had found it in an abandoned ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:We report the identification of acetone (0.45 mg/mL) and isopropanol (0.17 mg/mL) but without the presence of ethanol in a blood sample from a man suspected of driving under the influence of alcohol. A preliminary breath screening test with an electrochemical instrument (Alcolmeter S-L2) was positive and an evidential...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:For the past 10 years, medical imaging techniques have been increasingly applied to forensic investigations. To obtain histological and toxicological information, tissue and liquid samples are required. In this article, we describe the development of a low-cost, secure, and reliable approach for a telematic add-on for...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01990.x
更新日期：2012-03-01 00:00:00
abstract：:A procedure for the multisystem analysis of bloodstains using the simultaneous separation of the enzymes glyoxalase I, esterase D, and phosphoglucomutase has been developed. The amount of bloodstain required has therefore been reduced threefold without any loss in resolution and sensitivity. Bloodstains at least seven...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:A case is presented of a 53-year-old white male who successfully committed suicide by stabbing himself in the abdomen and chest, after which he was driven 11 km (seven miles) to a church without anybody noticing his wounds. In the church he finished his task by stabbing himself through the calvarium. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：:Suspects in shooting investigations in Chicago are routinely transported in department vehicles and detained in department facilities prior to gunshot residue (GSR) evidence collection. The GSR test results are used to associate the suspect with primary exposure to GSR. The potential for these vehicles and facilities ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00457.x
更新日期：2007-07-01 00:00:00
abstract：:The isolation, amplification, and characterization of human DNA from hematophagous (blood feeding) and necrophagous (carrion feeding) arthropods have been advanced significantly by the development of polymerase chain reaction (PCR) DNA sequencing methodologies. Historically, DNA technology has been successfully utiliz...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Self-embedding behavior (SEB) is the repeated insertion of sharp objects, such as needles or pins, into the soft tissues of abdomen, limbs, and other body parts. In this study, two cases of SEB were reported and the scientific worldwide literature reviewed. Thirty-two cases of SEB were identified through systematic se...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：10.1111/1556-4029.13359
更新日期：2017-07-01 00:00:00
abstract：:This study has shown that the combination of simple techniques with the use of multivariate statistics offers the potential for the comparative analysis of soil samples. Five samples were obtained from each of twelve state parks across New Jersey in both the summer and fall seasons. Each sample was examined using part...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12375
更新日期：2014-05-01 00:00:00
abstract：:Most false aneurysms of the heart represent contained ventricular free wall ruptures after myocardial infarction. Post-traumatic aneurysms also may follow penetrating or non-penetrating trauma to the chest. Regardless of the origin of the false aneurysm there is a propensity for aneurysm rupture. We report a patient w...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:A rapid and precise method for the isolation and identification of dihydrocodeine from urine is reported. The narcotic is isolated from urine using Sep-Pak C18 cartridges for cleanup, requiring less than 30 min for preparation. Identification is performed by gas chromatography/mass spectrometry. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:The RSID-saliva test and the SALIgAE-saliva test are two recently developed forensic saliva detection kits. In this study, we compared the sensitivity and the specificity of the two test kits with the Phadebas amylase test by analyzing amylases from various sources including human, animals, plants, and micro-organism....
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00814.x
更新日期：2008-09-01 00:00:00
abstract：:Owing to the scanty evidence on usefulness of information of appearance of gray hair for age estimation, this study was conducted to estimate age based on the appearance of gray hair on different body sites in a sample of autopsy cases in Sri Lanka. A descriptive cross-sectional study was conducted in Teaching Hospita...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13375
更新日期：2017-07-01 00:00:00
abstract：:Many studies have been published describing numerous techniques to determine the aging of individuals from dental tissues. However, few case reports demonstrate how such techniques can be employed by the forensic odontologist undertaking casework. Indeed, many of the techniques are highly complex and utilize equipment...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:Human fetal skeletal elements of different gestational ages were screened with multiple mesh sizes (6.4 mm [1/4 inch], 3.2 mm [1/8 inch], 2.0 mm, and 1.0 mm) to determine their recovery rates. All remains were previously macerated, and no significantly damaged elements were used. The 6.4 mm mesh allowed a large loss o...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12922
更新日期：2016-01-01 00:00:00
abstract：:Reported herein is the suicidal asphyxiation of a young man due to exhaustion of oxygen in the interior of a sealed automobile into which the exhaust emissions were diverted. His blood carboxyhemaglobin concentration was less than 5% saturation. The car was equipped with a catalytic converter and when tested, the exha...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00224.x
更新日期：2006-09-01 00:00:00
abstract：:Blood samples of two cases were analyzed preliminarily by a classical spectrophotometric method (VIS) and by an automated headspace gas chromatographic method with nitrogen-phosphorus detector (HS-GC/NPD). In the former, hydrogen cyanide (HCN) was quantitatively determined by measuring the absorbance of chromophores f...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00570.x
更新日期：2007-11-01 00:00:00
abstract：POPULATION:The distribution of allele frequencies have been analyzed at two short tandem repeat (STR) loci FES and D2S1328 among five anthropologically distinct ethnic groups of India namely Ezhavas, Nairs, Arayas, Vishwakarma and Muslims. Muslims are religio-ethnic group while other populations mentioned above belong ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:DNA evidence holds an important position in criminal investigations and proceedings. The polymerase chain reaction (PCR) is often utilized to amplify polymorphic regions of DNA which are subsequently typed to produce distinct genotypes. The sensitivity of PCR-based techniques provides a major advantage over other DNA ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
