Bisphenol A stimulates differentiation of rat stem Leydig cells in vivo and in vitro.

abstract：:The identification of XY females carrying a duplication of a region of the X chromosome (Xp21) led to the hypothesis that a double dose of a gene in the duplicated region causes sex reversal (DSS; dosage sensitive sex reversal). A gene isolated from this region, named DAX-1 (DSS-AHC critical region on the X), encodes ...

journal_title：Molecular and cellular endocrinology

authors： Parma P,Pailhoux E,Puissant C,Cotinot C

更新日期：1997-11-30 00:00:00

abstract：:Pituitary and plasma pools from postmenopausal women and plasma pools from women at midcycle were fractionated by electrofocusing in sucrose density gradients. The biological LH activity was determined in each of the electrofocusing fractions by the use of an in vitro bioassay method. A heterogeneous profile of LH act...

journal_title：Molecular and cellular endocrinology

authors： Robertson DM,Van Damme MP,Diczfalusy E

更新日期：1977-11-01 00:00:00

abstract：:Rat adipocytes in primary culture have been used to study the intracellular processing of growth hormone (GH) receptors. Pretreatment of adipocytes with 20 micrograms/ml cycloheximide resulted in a rapid decline (t1/2 approximately 45 min) of the 125I-human growth hormone (hGH) binding capacity of the cells. This decl...

journal_title：Molecular and cellular endocrinology

authors： Roupas P,Herington AC

更新日期：1988-05-01 00:00:00

abstract：:The endocrine function of the ovary is dependent upon the ovarian follicle, which on a cellular basis consists of an oocyte surrounded by adjacent somatic cells responsible for generating sex steroid hormones and maintenance of hormonal stasis with the hypothalamic-pituitary axis. As females age, both fertility and th...

journal_title：Molecular and cellular endocrinology

authors： Truman AM,Tilly JL,Woods DC

更新日期：2017-04-15 00:00:00

abstract：:Immature rat testes contain a specific binding protein for testosterone (T) and 5alpha-dihyrotestosterone (DHT) with physico-chemical properties similar to the cytoplasmic androgen receptors in the epididymis and ventral prostate but different from the testicular androgen-binding protein (ABP). Like the androgen recep...

journal_title：Molecular and cellular endocrinology

authors： McLean WS,Smith AA,Hansson V,Naess O,Nayfeh SN,French FS

更新日期：1976-03-01 00:00:00

abstract：:Porcine Leydig cells in primary culture were incubated to equilibrium with increasing doses of either porcine luteinizing hormone (pLH) or human chorionic gonadotropin (hCG). Then, free and total high affinity gonadotropin receptors on the cell surface were dosed with [125I]hCG as tracer. In isotonic high salt medium,...

journal_title：Molecular and cellular endocrinology

authors： Genty N,Salesse R,Jallal B,Garnier J

更新日期：1991-04-01 00:00:00

abstract：:The mechanisms of cytokine-induced beta-cell death are poorly characterised. In rat insulin-producing RINm5F cells, the combination of interleukin-1beta, interferon-gamma and tumour necrosis factor-alpha presently induced disruption of the mitochondrial membrane potential (Deltapsi(m)) as demonstrated by reduced JC-1 ...

journal_title：Molecular and cellular endocrinology

authors： Barbu A,Welsh N,Saldeen J

更新日期：2002-04-25 00:00:00

abstract：:PTH/PTHrP receptor gene expression was evaluated in situ in avian epiphyseal growth plates taken from normal, rachitic and tibial dyschondroplasia (TD) afflicted chicks induced by thiram or by genetic selection. In the normal growth plates, PTH/PTHrP receptor gene expression was localized to the maturation zone as dem...

journal_title：Molecular and cellular endocrinology

authors： Ben-Bassat S,Genina O,Lavelin I,Leach RM,Pines M

更新日期：1999-03-25 00:00:00

abstract：:The expression of the three genes encoding the components C1, C2 and C3 of prostatic binding protein (PBP) is under androgen control and restricted to the rat ventral prostate. The SstI-PvuII fragment of the first intron of the C3(1) gene displays two binding sites for ubiquitous transcription factors and one for a ti...

journal_title：Molecular and cellular endocrinology

authors： Celis L,Claessens F,Peeters B,Heyns W,Verhoeven G,Rombauts W

更新日期：1993-08-01 00:00:00

abstract：:Single-chain gonadotropin analogs had been constructed for the purpose of structure-function studies and analog design. Incorporation of a spacer derived from the carboxyl terminal peptide (CTP) of the choriogonadotropin (CG) beta subunit between the tethered subunit domains of the human gonadotropins is beneficial fo...

journal_title：Molecular and cellular endocrinology

authors： Braw-Tal R,Pen S,Grinberg M,Nakav S,Ben-Menahem D

更新日期：2006-06-27 00:00:00

abstract：:Specific binding sites for bovine placental lactogen (bPL) and the lactogenic hormone, prolactin, have been detected in endometrial membranes isolated from uteri of mid-pregnant heifers. The specific binding of human growth hormone (hGH) (used to monitor the presence of lactogenic binding sites) and of bPL was increas...

journal_title：Molecular and cellular endocrinology

authors： Galosy SS,Gertler A,Elberg G,Laird DM

更新日期：1991-07-01 00:00:00

abstract：:In order to study the intracellular cAMP response kinetics of Leydig cells to hormones with LH activity, we used MLTC-1 cells transiently expressing a chimeric cAMP-responsive luciferase so that real-time variations of intracellular cAMP concentration could be followed using oxiluciferin luminescence produced from cat...

journal_title：Molecular and cellular endocrinology

authors： Klett D,Meslin P,Relav L,Nguyen TM,Mariot J,Jégot G,Cahoreau C,Combarnous Y

更新日期：2016-10-15 00:00:00

abstract：:Insulin and cAMP stimulate prolactin gene transcription and prolactin-CAT expression in rat pituitary tumor GH cells. Expression of prolactin-CAT construct, pPrl(-173/+75)CAT, is stimulated 10- to 30-fold by either insulin or cAMP. Addition of both insulin and cAMP resulted in an additive 20- to 60-fold stimulation. A...

journal_title：Molecular and cellular endocrinology

authors： Jacob KK,Stanley FM

更新日期：1995-04-01 00:00:00

abstract：:SF-1 (steroidogenic factor-1) (NR5A1) and DAX-1 (dosage-sensitive sex-reversal, adrenal hypoplasia congenital, X chromosome) (NR0B1) are orphan nuclear receptors that are expressed in the adrenal gland, gonads, ventromedial hypothalamus (VMH), and pituitary gonadotrope cells. The function of these genes has been clari...

journal_title：Molecular and cellular endocrinology

authors： Achermann JC,Meeks JJ,Jameson JL

更新日期：2001-12-20 00:00:00

abstract：:Rat ovarian membrane luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor was reconstituted into proteoliposomes. The ability of sodium cholate to extract and reconstitute hCG binding activity was dependent on the protein/detergent ratio. Trypsinization of the LH/hCG receptor containing proteoliposomes i...

journal_title：Molecular and cellular endocrinology

authors： Kolena J,Matejcíková K,Srenkelová G

更新日期：1992-02-01 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. The hormonal regulation of this protein was studied both in vitro and in vivo. In mammary gland explant culture, levels of the MMTV binding protein were maintained onl...

journal_title：Molecular and cellular endocrinology

authors： Bolander FF Jr

更新日期：1991-12-01 00:00:00

abstract：:Recent evidence using GT1-7 cells indicates that GnRH pulsatility depends on exocytotic-release and gene transcription events. To determine whether calcium or DREAM may play a role in linking these processes, we used an L-type Ca(2+)-blocker (nimodipine) and found that not only GnRH gene expression (GnRH-GE) pulse act...

journal_title：Molecular and cellular endocrinology

authors： Leclerc GM,Boockfor FR

更新日期：2007-03-15 00:00:00

abstract：:Presently, a growing interest is focused on the origins of the "Metabolic Syndrome", a cluster of several metabolic disorders linking obesity, dyslipidemia, hypertension and type 2 diabetes mellitus. Clearly, genetic predisposition and deleterious lifestyle, including low physical activity and hypercaloric alimentatio...

journal_title：Molecular and cellular endocrinology

authors： Somm E,Schwitzgebel VM,Vauthay DM,Aubert ML,Hüppi PS

更新日期：2009-05-25 00:00:00

abstract：:Two cAMP-dependent protein kinase activities can be separated from rat thyroid cytosol. Their elution properties during the gel chromatography, as well as their sedimentation coefficients after sucrose-gradient ultracentrifugation and their capacity to dissociate in the presence of histones, suggest they are like the ...

journal_title：Molecular and cellular endocrinology

authors： Delbauffe D,Ohayon R,Pavlovic-Hournac M

更新日期：1979-05-01 00:00:00

abstract：:Isolated islets were either studied immediately after isolation (fresh; F), or were cultured for 6 days at 11 mM glucose (desensitized; D), or were incubated for 2 h at 5.5 mM glucose following D (recovered; R). Glucose-stimulated insulin secretion in D islets was reduced compared with F and R islets. In the presence ...

journal_title：Molecular and cellular endocrinology

authors： Laychock SG

更新日期：1995-08-30 00:00:00

abstract：:The antiestrogen tamoxifen has been successfully used to control estrogen receptor (ER) and progesterone receptor positive breast cancer. However, the development of antiestrogen resistance is frequently observed in patients following long term treatment. We have studied the development of antiestrogen resistance in v...

journal_title：Molecular and cellular endocrinology

authors： Jiang SY,Wolf DM,Yingling JM,Chang C,Jordan VC

更新日期：1992-12-01 00:00:00

abstract：:Platelet-derived growth factor (PDGF) is a potent mitogen for several cell types. In addition, PDGF has vasoconstrictive action and shares some signal transduction mechanisms with angiotensin II (AII). In the present study, we have examined the effects of PDGF on basal and AII-induced aldosterone synthesis in freshly ...

journal_title：Molecular and cellular endocrinology

authors： Natarajan R,Nadler J

更新日期：1992-01-01 00:00:00

abstract：:We studied the influence of thyroid hormones and vitamin A status on the regulation of UDP-glucuronosyltransferase (UGT) expression and the glucuronidation of thyroid hormones by UGTs. For this, we used an original model of rats fed with different vitamin A diets and implanted subcutaneously by osmotic minipumps deliv...

journal_title：Molecular and cellular endocrinology

authors： Haberkorn V,Heydel JM,Mounie J,Artur Y,Goudonnet H

更新日期：2002-04-25 00:00:00

abstract：:The peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily. These ligand-activated transcription factors are implicated in the regulation of lipid metabolism and adipocyte differentiation and in the regulation of anti-inflammatory processes. In order to bind to DNA ...

journal_title：Molecular and cellular endocrinology

authors： Gorla-Bajszczak A,Juge-Aubry C,Pernin A,Burger AG,Meier CA

更新日期：1999-01-25 00:00:00

abstract：:In this study, we determined the ligand-dependent activation function domain 2 (AF-2) of the human vitamin D receptor (hVDR) and characterized it using site-directed mutagenesis. A single mutation at glutamic acid-420 (E420Q) and an additional mutation at leucine-417 (L417A-E420Q) eliminated ligand-dependent transcrip...

journal_title：Molecular and cellular endocrinology

authors： Nakajima S,Yamagata M,Sakai N,Ozono K

更新日期：1998-04-30 00:00:00

abstract：:The effect of luteinising hormone (LH) on bovine cervical tissues from three phases of the estrous cycle was studied. It was found that in the luteal phase cervix contained an LH receptor mRNA transcript and the 93 kDa LH receptor protein. Incubation of cervical minces from luteal phase with LH significantly increased...

journal_title：Molecular and cellular endocrinology

authors： Mizrachi D,Shemesh M

更新日期：1999-11-25 00:00:00

abstract：:The effect of thyroid status on the postnatal development of the two molecular forms of Na+,K+-ATPase, distinguished kinetically on the basis of their ouabain sensitivity, was examined in rat brain. Hypothyroidism induced by PTU from day 1 postnatally significantly reduced the Na+,K+-ATPase activity in cerebellum (22-...

journal_title：Molecular and cellular endocrinology

authors： Atterwill CK,Reid J,Athayde CM

更新日期：1985-05-01 00:00:00

abstract：:The juvenoid, methyl farnesoate (MF), is synthesized in the mandibular organs (MOs) of crustaceans, under the control of mandibular organ-inhibiting hormone (MO-IH). Using an in vitro assay to measure synthesis of MF by MOs, the effect of a variety of agents that affect signal transduction pathways was investigated. O...

journal_title：Molecular and cellular endocrinology

authors： Wainwright G,Webster SG,Rees HH

更新日期：1999-08-20 00:00:00

abstract：:The ovarian granulosa cell has previously been shown to be a site of insulin-like growth factor (IGF) I production, reception, and action. It is the objective of this study to characterize in greater detail the soluble IGF binding activity released by this cell type. To this end, use was made of granulosa cells from i...

journal_title：Molecular and cellular endocrinology

authors： Adashi EY,Resnick CE,Hernandez ER,Hurwitz A,Rosenfeld RG

更新日期：1990-12-21 00:00:00

abstract：:The high-density lipoprotein (HDL) pathway in rat adrenocortical cells was studied at the electron microscopic level in vitro via colloidal gold labelling. Steroid hormone assays were performed to confirm that the cells remained intact, viable, responsive to ACTH under the applied conditions, and to reveal the steroid...

journal_title：Molecular and cellular endocrinology

authors： Tóth IE,Szabó D,Bácsy E,Szalay KS,Hesz A,Szollár LG

更新日期：1986-02-01 00:00:00