Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species.

Abstract:

:Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H2O2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H2O2. We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of α-synuclein and pathophysiological H2O2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.

journal_name

R Soc Open Sci

authors

Needham LM,Weber J,Fyfe JWB,Kabia OM,Do DT,Klimont E,Zhang Y,Rodrigues M,Dobson CM,Ghandi S,Bohndiek SE,Snaddon TN,Lee SF

doi

10.1098/rsos.171399

subject

Has Abstract

pub_date

2018-02-07 00:00:00

pages

171399

issue

2

issn

2054-5703

pii

rsos171399

journal_volume

5

pub_type

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