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Conserved mechanism of cell-wall synthase regulation revealed by the identification of a new PBP activator in Pseudomonas aeruginosa.

Abstract：

:Penicillin-binding proteins (PBPs) are synthases required to build the essential peptidoglycan (PG) cell wall surrounding most bacterial cells. The mechanisms regulating the activity of these enzymes to control PG synthesis remain surprisingly poorly defined given their status as key antibiotic targets. Several years ago, the outer-membrane lipoprotein EcLpoB was identified as a critical activator of Escherichia coli PBP1b (EcPBP1b), one of the major PG synthases of this organism. Activation of EcPBP1b is mediated through the association of EcLpoB with a regulatory domain on EcPBP1b called UB2H. Notably, Pseudomonas aeruginosa also encodes PBP1b (PaPBP1b), which possesses a UB2H domain, but this bacterium lacks an identifiable LpoB homolog. We therefore searched for potential PaPBP1b activators and identified a lipoprotein unrelated to LpoB that is required for the in vivo activity of PaPBP1b. We named this protein LpoP and found that it interacts directly with PaPBP1b in vitro and is conserved in many Gram-negative species. Importantly, we also demonstrated that PaLpoP-PaPBP1b as well as an equivalent protein pair from Acinetobacter baylyi can fully substitute for EcLpoB-EcPBP1b in E. coli for PG synthesis. Furthermore, we show that amino acid changes in PaPBP1b that bypass the PaLpoP requirement map to similar locations in the protein as changes promoting EcLpoB bypass in EcPBP1b. Overall, our results indicate that, although different Gram-negative bacteria activate their PBP1b synthases with distinct lipoproteins, they stimulate the activity of these important drug targets using a conserved mechanism.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Greene NG,Fumeaux C,Bernhardt TG

doi

10.1073/pnas.1717925115

subject

Has Abstract

pub_date

2018-03-20 00:00:00

pages

3150-3155

issue

12

eissn

0027-8424

issn

1091-6490

pii

1717925115

journal_volume

115

pub_type

杂志文章

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