Abstract:
:Using an 18 week two-stage protocol we have compared the tumour-promoting properties of a range of phorbol and daphnane esters on female CD-1 mice. The induction of epidermal hyperplasia in this mouse strain by these compounds has also been assessed by comparison with the standard phorbol ester, 12-O-tetradecanoylphorbol-13-O-acetate (TPA). Two compounds, sapintoxin D (SAP D) and thymeleatoxin A (TA) (a daphnane structurally related to the second-stage promoter mezerein) were shown to be second-stage promoters using 5 nmol TPA as a first-stage promoter and 0.2 mumol 7,12-dimethylbenz[a]anthracene (DMBA) as initiator. Both compounds at a dose of 17 nmol were hyperplasiogenic. Two further derivatives, sapintoxin C (SAP C) and 4 alpha-sapinine (alpha-SAP) were inactive as promoters and hyperplastic agents. 4 alpha-sapinine, which prevents in vitro stimulation of protein kinase C (PKC), by 12-O-tetradecanoylphorbol-13-O-acetate (TPA) failed to inhibit significantly TPA-induced promotion and hyperplasia at a dose of 20 nmol and 100 nmol respectively. Sapintoxin A (SAP A), a potent activator of PKC, was neither a complete nor second-stage promoter at doses of up to 20 nmol. A series of in vivo and in vitro experiments which were carried out to determine the metabolic fate of this compound under experimental conditions showed that SAP A was not metabolized to any significant extent up to 48 h. When SAP A was co-administered with sub-hyperplastic doses of the calcium ionophore A23187 (5 micrograms and 10 micrograms) tumours appeared in a dose-dependent manner. This combination was also hyperplasiogenic in mouse skin. SAP A may be a useful probe for studying the involvement of PKC isozymes in tumour promotion and cell proliferation.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Brooks G,Evans AT,Aitken A,Evans FJdoi
10.1093/carcin/10.2.283subject
Has Abstractpub_date
1989-02-01 00:00:00pages
283-8issue
2eissn
0143-3334issn
1460-2180journal_volume
10pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Styrene oxide was reacted with guanosine in fully aqueous conditions, and the products were purified by cation exchange chromatography and h.p.l.c. The products were characterized by u.v. and mass spectra, loss of tritium from the C-8 position and dissociation properties. About 90% of the total product was identified,...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.5.601
更新日期:1984-05-01 00:00:00
abstract::Crude plasma membranes were prepared from the liver of control rats or of rats submitted to an initiation by diethyl-nitrosamine and selection with 2-acetylaminofluorene and carbon tetrachloride (group IS) or of rats submitted to an initiation-selection protocol followed by a promotion with phenobarbital (group IS PB)...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.4.659
更新日期:1991-04-01 00:00:00
abstract::Acrylamide is an alkylating agent which reacts very slowly in direct reactions with DNA and is negative in the Ames test, but is carcinogenic in mice and rats. In order to explain the cancer-initiating properties of acrylamide we have studied DNA adduct formation in vitro with a metabolizing system and in vivo in mice...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.5.1161
更新日期:1995-05-01 00:00:00
abstract::The steroid hormone 1,25-dihydroxyvitamin D(3), [1,25(OH)(2)D(3), calcitriol], the active metabolite of vitamin D, exerts pleiotropic antitumor effects against several malignancies. However, the clinical use of this hormone is limited by hypercalcemia. 25-Hydroxyvitamin D(3), the prohormone of 1,25(OH)(2)D(3), is hydr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh086
更新日期:2004-06-01 00:00:00
abstract::Mutations in codon 61 of the Ha-ras proto-oncogene have been shown to occur with a high frequency in both spontaneous and carcinogen-induced liver tumours of the B6C3F1 mouse. In the present study we analysed the frequency and pattern of Ha-ras mutations in liver tumours in a different strain of mice, namely the CF1 m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.10.1875
更新日期:1990-10-01 00:00:00
abstract::Thioredoxin reductases (TrxRs) catalyse the NADPH-dependent reduction of thioredoxin and play an important role in multiple cellular events related to carcinogenesis including cell proliferation, apoptosis and cell signaling. We have used human hepatoma HepG2 cells to examine the regulation of TrxRs by isothiocyanate ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.3.497
更新日期:2003-03-01 00:00:00
abstract::A large number of chemical carcinogens require metabolic activation before they are biologically active. The metabolism of a few of these compounds has been investigated in cultured human tissues and cells and the metabolism has been compared with the metabolism in organs from experimental animals in which the compoun...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/11.5.707
更新日期:1990-05-01 00:00:00
abstract::We investigated, in a cloned hamster tracheal epithelial cell line HTE-B, the effects of inhibitors of DNA topoisomerase, novobiocin and nalidixic acid; of DNA polymerase, 1-beta-arabinofuranosylcytosine (ara-C) and 2',3'-dideoxythymidine; of ribonucleotide reductase, hydroxyurea; and of poly(ADP-ribose)synthetase, 3-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.2.187
更新日期:1984-02-01 00:00:00
abstract::Alterations of epigenetic modifications are promising targets for cancer therapy, and several epigenetic drugs are now being clinically utilized. At the same time, individual epigenetic modifications have physiological functions in normal cells, and cancer cell specificity is considered difficult to achieve using a dr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu238
更新日期:2015-02-01 00:00:00
abstract::An in vivo method for assessment of DNA adduct formation and unscheduled DNA synthesis (UDS) in the esophagus of rats was devised. Small ventral incisions were made in the neck and upper abdomen regions of 6 week old F344 rats and ligation of the esophagus with thread at the two extreme ends performed to make an esoph...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.2.235
更新日期:1990-02-01 00:00:00
abstract::We have studied the influence of genetic polymorphisms in the xenobiotic-metabolizing genes GSTM1, GSTP1, GSTT1, EPHX1, NAT1 and NAT2 and the folate-metabolizing genes MTR and MTHFR on the frequencies of cells with chromosomal aberrations (CAs) in peripheral lymphocytes of Norwegian men. Log-linear Poisson regression ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq246
更新日期:2011-03-01 00:00:00
abstract::Isolated trout liver cells were treated with lysolecithin to produce an in situ system for characterizing DNA repair in teleosts. In this preparation, the integrity of the plasma membrane is altered, nuclei remain intact, and the concentrations of dNTPs and nucleotide analogs, which normally do not penetrate intact pl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.6.995
更新日期:1989-06-01 00:00:00
abstract::To test the hypothesis that polymorphic variants of antioxidant genes modify the risk of pancreatic cancer, we examined seven single-nucleotide polymorphisms (SNPs) of genes coding for superoxide dismutase (SOD) 2, glutathione S-transferase alpha 4 (GSTA4), catalase and glutathione peroxidase in 575 patients with panc...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp310
更新日期:2010-04-01 00:00:00
abstract::Male Sprague-Dawley rats were fed a cube diet containing 2.51 mmol/kg 3'-hydroxymethyl-N,N-dimethyl-4-aminoazobenzene (3'-CH2OH-DAB) for a period of 1 or 3 months. (This is the molar equivalent of 0.06% 3'-Me-DAB in the diet). The oral administration of 3'-CH2OH-DAB for 3 months resulted in a high incidence of liver t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.6.533
更新日期:1980-06-01 00:00:00
abstract::Previous studies from our laboratory demonstrated that diallyl disulfide (DADS), an oil-soluble allyl sulfur compound found in processed garlic, markedly suppressed p34(cdc2) kinase activity and induced a G(2)/M phase arrest in cultured human colon tumor (HCT-15) cells. The present studies reveal that suppression of p...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::CXCL5 is a member of the CXC-type chemokine family that may play a role in carcinogenesis and cancer progression. This study investigates the biological function and clinical significance of CXCL5 in intrahepatic cholangiocarcinoma (ICC). We demonstrated that CXCL5 was overexpressed in ICC cell lines and tumor samples...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt397
更新日期:2014-03-01 00:00:00
abstract::An antibody (anti-EH) specific for microsomal epoxide hydrolase (mEH) from rhesus monkey liver has been used to test the immunochemical relationship between human liver mEH and the serum EH levels in human patients with hepatocellular carcinoma (HCC). Immunoblots of separated rhesus monkey and human liver microsomal p...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.2.343
更新日期:1989-02-01 00:00:00
abstract::A growing body of evidence from human and animal cancer cytogenetics studies indicates that aneuploidy is an important chromosome change in carcinogenesis. To understand the role of this genetic phenomenon during the first steps of an experimental cancer model, molecular and cellular techniques were combined. A sequen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.8.1825
更新日期:1995-08-01 00:00:00
abstract::In order to explore a possibility that the custom of drinking green tea infusion is efficacious for reducing the carcinogenic risk of environmental exposure to pentachlorophenol (PCP), we examined the effects in a hepato- and cholangiocarcinogenesis model in mice exposed to diethylnitrosamine (DEN). In the first exper...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg053
更新日期:2003-06-01 00:00:00
abstract::Rats received a choline-deficient diet containing 0.1% (w/w) DL-ethionine (CDE) for 4, 10, 14 or 22 weeks. A separate group was treated for 4 weeks with CDE and then received a normal diet for 4 weeks. The L and M2 isoenzymes of pyruvate kinase were immunocytochemically demonstrated in liver sections. L-PK expression ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.1.99
更新日期:1998-01-01 00:00:00
abstract::This study was conducted to investigate the modifying effect of glutathione S-transferase (GST) M1 and T1 polymorphisms on aflatoxin-induced hepatocarcinogenesis among chronic hepatitis B virus surface antigen (HBsAg) carriers. A total of 79 HBsAg-positive cases of hepatocellular carcinoma (HCC) diagnosed between 1991...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.8.1289
更新日期:2001-08-01 00:00:00
abstract::Cancer health disparities remain stubbornly entrenched in the US health care system. The Affordable Care Act was legislation to target these disparities in health outcomes. Expanded access to health care, reduction in tobacco use, uptake of other preventive measures and cancer screening, and improved cancer therapies ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa121
更新日期:2020-11-13 00:00:00
abstract::In light of clinical and biological evidence that bile constituents exert preventive effects against colorectal cancer, we evaluated the influence of oral bilirubin and sodium taurocholate (NaTC) on intestinal tumor formation in APC(Min/+) mice. Mice received bilirubin and/or bovine serum albumin (BSA) and NaTC in the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq050
更新日期:2010-06-01 00:00:00
abstract::Copenhagen (Cop) rats are completely resistant to the chemical induction of mammary adenocarcinomas, but their susceptibility to hepatocarcinogenesis is virtually unknown. Rat liver is a well-characterized and easily manipulated tissue in which to study carcinogenesis. Therefore, if Cop rats are resistant to hepatocar...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.9.1745
更新日期:1997-09-01 00:00:00
abstract::Prior work has demonstrated that food restriction of mice markedly suppresses 12-O-tetradecanoylphorbol-13-acetate (TPA) promotion of skin papillomas and adrenalectomy prior to initiating food restriction completely reverses the tumor inhibitory effect of underfeeding. In the present experiment the effect of food rest...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.2.209
更新日期:1996-02-01 00:00:00
abstract::When exogenous [(3)H]retinol (vitamin A) was added to culture medium, normal human epithelial cells from the oral cavity, skin, lung and breast took up and esterified essentially all of the [(3)H]retinol within a few hours. As shown by [(3)H]retinol pulse-chase experiments, normal epithelial cells then slowly hydrolyz...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.11.1925
更新日期:2000-11-01 00:00:00
abstract::N-Nitrosodimethylamine (NDMA) is a potent mutagen and animal carcinogen to which many people are exposed through the consumption of contaminated food and the use of tobacco products. Although the mutational specificity of NDMA has been studied in bacteria, little is known about the specific types of mutations induced ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.5.755
更新日期:1998-05-01 00:00:00
abstract::Carcinogenicity of catechol, a naturally occurring and industrial chemical which has been shown to have strong cell proliferating potential on rat glandular stomach epithelium, was investigated in male and female F344 rats and B6C3F1 mice. Groups of 30 male and female F344 rats and B6C3F1 mice were treated with 0.8% c...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.3.525
更新日期:1993-03-01 00:00:00
abstract::The modulating effects of dietary feeding of two xanthophylls, astaxanthin (AX) and canthaxanthin (CX) during the postinitiation phase on colon carcinogenesis initiated with azoxymethane (AOM) were investigated in male F344 rats. Animals were initiated with AOM by weekly s.c. injections of 15 mg/kg body wt for 3 weeks...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.12.2957
更新日期:1995-12-01 00:00:00
abstract::Lipid peroxidation aldehydes of the 4-hydroxy-alpha, beta-unsaturated type, as well as the tobacco-smoke related alpha, beta-unsaturated aldehyde, acrolein, were highly cytotoxic and decreased the intracellular thiol content in cultured human bronchial fibroblasts after treatment with micromolar concentrations. In com...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.12.1755
更新日期:1985-12-01 00:00:00