Abstract：
BACKGROUND AND AIMS:The mechanisms that drive atherosclerotic plaque progression and destabilization in humans remain largely unknown. Laboratory models are needed to study these mechanisms under controlled conditions. The aim of this study was to establish a new ex vivo model of human atherosclerotic plaques that preserves the main cell types in plaques and the extracellular components in the context of native cytoarchitecture. METHODS:Atherosclerotic plaques from carotid arteries of 28 patients undergoing carotid endarterectomy were dissected and cultured. At various time-points, samples were collected and analysed histologically. After enzymatic digestion, single cells were analysed with flow cytometry. Moreover, tissue cytokine production was evaluated. RESULTS:We optimised the plaque dissection protocol by cutting plaques into circular segments that we cultured on collagen rafts at the medium-air interface, thus keeping them well oxygenated. With this technique, the relative presence of T and B lymphocytes did not change significantly during culture, and the sizes of lymphocyte subsets remained stable after day 4 of culture. Macrophages, smooth muscle cells, and fibroblasts with collagen fibres, as well as T and B lymphocyte subsets and CD16 natural killer cells, remained largely preserved for 19 days of culture, with a continuous production of inflammatory cytokines and chemokines. CONCLUSIONS:Our new model of ex vivo human atherosclerotic plaques, which preserves the main subsets of immune cells in the context of tissue cytoarchitecture, may be used to investigate important aspects of atherogenesis, in particular, the functions of immune cells under controlled laboratory conditions.
journal_name
Atherosclerosisjournal_title
Atherosclerosisauthors
Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis Ldoi
10.1016/j.atherosclerosis.2017.10.003subject
Has Abstractpub_date
2017-12-01 00:00:00pages
90-98eissn
0021-9150issn
1879-1484pii
S0021-9150(17)31323-0journal_volume
267pub_type
杂志文章相关文献
ATHEROSCLEROSIS文献大全abstract：OBJECTIVE:The plasma level of soluble CD40 ligand (sCD40L), which induces pro-inflammatory and pro-atherogenic responses, is known to be elevated in atherosclerotic patients. In this study, we investigated the mechanism of sCD40L release from human platelets, focusing on the involvement of thromboxane (TX) A(2). METHO...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2009.10.024
更新日期：2010-04-01 00:00:00
abstract：:In previous works, it has been evidenced that lipoperoxidative injury to macrophages caused by oxidatively modified low-density lipoprotein (O-LDL) plays an important role in foam cell formation, and that PSK, a protein bound polysaccharide extracted from the class Basidiomycetes Coriolus Versicolor, can protect macro...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(96)05835-2
更新日期：1996-08-02 00:00:00
abstract：:When smoke from single cigarettes containing [4-14C]cholesterol or beta-[4-14C]sitosterol was delivered to the lungs of Rhesus macaques, plasma contained radiolabeled sterols up to 50 days later. Since cholesterol, as well as plant sterols (campesterol, stigmasterol and beta-sitosterol), are normally present in cigare...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(86)90082-1
更新日期：1986-04-01 00:00:00
abstract：RATIONALE:Obstructive sleep apnea results in nocturnal intermittent hypoxia (IH) as a main trigger for cardiovascular morbidity, including atherosclerosis. IH induces hemodynamic, hormono-metabolic and also immuno-inflammatory alterations that could differentially contribute to atherosclerosis. Our study aimed at exami...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2011.07.122
更新日期：2011-12-01 00:00:00
abstract：:Oxidation of low density lipoprotein (LDL) is implicated in the pathogenesis of atherosclerosis. In this study the susceptibility to oxidation of LDL (from patients with atherosclerosis) is related to the progression of the disease. LDL were isolated from 37 patients with demonstrable atherosclerotic plaques. The susc...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(94)05401-4
更新日期：1995-01-06 00:00:00
abstract：:Preclinical and clinical data on atorvastatin, a new 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, indicate that it has superior activity in treating a variety of dyslipidemic disorders characterized by elevations in low-density lipoprotein cholesterol (LDL-C) and/or triglycerides. Results for p...
journal_title：Atherosclerosis
pub_type： 杂志文章,评审
doi：10.1016/s0021-9150(97)06066-8
更新日期：1997-05-01 00:00:00
abstract：OBJECTIVE:To investigate the statin eligibility and the predictabilities for cardiovascular disease between AHA/ACC and ATPIII guidelines, comparing those results to concomitant coronary artery calcium scores (CACS) in a large cohort of Korean individuals who met statin-eligibility criteria. METHODS:Among 19,920 parti...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2015.03.006
更新日期：2015-05-01 00:00:00
abstract：:We have previously reported that mouse peritoneal macrophages have three types of modified low density lipoprotein (LDL) receptors. One is specific for acetylated LDL (Ac-LDL), the second is for oxidized LDL (Ox-LDL), and the third recognizes both (Arai, H. et al. (1989) Biochem. Biophys. Res. Commun. 159, 1375-1382)....
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(93)90098-f
更新日期：1993-06-01 00:00:00
abstract：BACKGROUND AND AIMS:Exercise training (ET) helps treat atherosclerosis. However, many patients stop regular ET for various reasons. The effect of detraining on atherosclerosis is not well studied. We examined the effects of ET vs. short-term detraining on atheromatous matrix-metalloproteinase (MMP) activity in preexist...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2020.02.022
更新日期：2020-04-01 00:00:00
abstract：BACKGROUND AND AIM:To determine the incremental diagnostic value of epicardial adipose tissue (EAT) volume in addition to the coronary artery calcium (CAC) score for detecting hemodynamic significant coronary artery disease (CAD). METHODS AND RESULTS:122 patients (mean age 61 ± 10 years, 61% male) without a previous c...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2015.07.005
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND:CETP inhibitors block the transfer of cholesteryl ester from HDL-C to VLDL-C and LDL-C, thereby raising HDL-C and lowering LDL-C. In this study, we explored the effect of CETP inhibitors on hepatic LDL receptor (LDLR) and PCSK9 expression and further elucidated the underlying regulatory mechanism. RESULTS:W...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2014.05.931
更新日期：2014-08-01 00:00:00
abstract：:This study assessed the efficacy of rosuvastatin for reducing plasma low-density lipoprotein (LDL) cholesterol after 18 weeks of open-label, forced titration in patients with homozygous familial hypercholesterolaemia (hoFH) and compared the efficacy of rosuvastatin 80 mg and atorvastatin 80 mg. Forty-four patients age...
journal_title：Atherosclerosis
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.atherosclerosis.2007.06.028
更新日期：2008-03-01 00:00:00
abstract：:The effects of lovastatin therapy on concentrations and compositions of lipoproteins were examined in 14 patients with heterozygous familial hypercholesterolemia. The drug lowered plasma levels of cholesterol in total plasma, very low density + intermediate density lipoproteins (VLDL + IDL) and low density lipoprotein...
journal_title：Atherosclerosis
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0021-9150(90)90079-x
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:Given the role of estrogen in the regulation of lipid metabolism, we screened for functional polymorphisms in the estrogen receptor alpha (ER*), and examined for their influence on serum cholesterol. METHODS AND RESULTS:We identified a novel C>T polymorphism (ERNE-145), with a minor allele frequency of 41%. ...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2008.02.012
更新日期：2008-08-01 00:00:00
abstract：:Interferon gamma (IFN-gamma) is an immunomodulatory and anti-microbial cytokine, which has a variety of proatherogenic effects. It has been reported that IFN-gamma can down-regulate ABCA1 expression. However, its mechanism is elusive. In the present study, we have investigated the effect of IFN-gamma on ABCA1 expressi...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2008.07.029
更新日期：2009-04-01 00:00:00
abstract：:Rabbits fed a commercial chow diet containing 0.5% cholesterol and 14% coconut oil developed more severe hyperlipidemia and atherosclerosis than rabbits fed the same diet containing olive oil in place of coconut oil. Average plasma cholesterol was twice as high in the coconut oil/cholesterol-fed rabbits than in olive ...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(88)90142-6
更新日期：1988-06-01 00:00:00
abstract：BACKGROUND:It is unclear whether individuals experience specific patterns of morbidity prior to premature death from cardiovascular disease (CVD). METHODS:We examined morbidity levels in the 6 years leading up to death from CVD in 37,397 men and 113,198 women under 65 years of age from the Finnish Public Sector study,...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2010.04.013
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:The prolactin regulatory element binding (PREB) protein is a transcriptional factor that regulates prolactin promoter activity in rat anterior pituitary. It is expressed not only in the anterior pituitary but also in the cardiovascular system, including in human umbilical vascular endothelial cells (HUVECs). ...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2009.03.051
更新日期：2009-11-01 00:00:00
abstract：:The insertion/deletion (I/D) polymorphism of the ACE gene may be involved in structural arterial changes. Aim of the present study was to assess the relationship between the ACE I/D gene and vessel wall stiffness among older adults. The study was conducted within the Rotterdam study, a population-based cohort study in...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2004.01.012
更新日期：2004-05-01 00:00:00
abstract：BACKGROUND AND AIMS:Cell adhesion molecules (CAMs) are implicated in the initiation and progression of atherosclerosis, but their association with risk of type 2 diabetes remains inconsistent. This meta-analysis aimed to quantify this association with dose-response analysis in the general population without type 2 diab...
journal_title：Atherosclerosis
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.atherosclerosis.2019.06.908
更新日期：2019-08-01 00:00:00
abstract：OBJECTIVE:Interruption of the enterohepatic circulation of bile acids induces hepatic bile acid synthesis, increases hepatic cholesterol demand, and increases clearance of apoB-containing lipoproteins in plasma. Based on these effects, bile acid sequestrants have been used for many years to treat hypercholesterolemia a...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2013.05.017
更新日期：2013-08-01 00:00:00
abstract：:We investigated the effects of estrogen and simvastatin, administered both alone and in combination, on the plasma lipid levels and lipoprotein-related enzymes in 45 postmenopausal women with type IIa hypercholesterolemia. They received 0.625 mg conjugated equine estrogen (n=15), 5 mg simvastatin (n=15), or the combin...
journal_title：Atherosclerosis
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0021-9150(99)00350-0
更新日期：2000-05-01 00:00:00
abstract：:It has been reported that total cholesterol (Chol) response to probucol is greater in familial hypercholesterolemic (FH) patients with apo E4 than in those without apo E4. We further examined the effect of probucol on plasma triglyceride (TG) and lipoprotein-Chol levels as well as total Chol levels in heterozygous FH ...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(90)90007-6
更新日期：1990-09-01 00:00:00
abstract：:In response to homocysteine induced toxicity in human umbilical vein endothelial cells, minimal changes in the concentration of cellular protein thiols but substantial changes in the concentration of intracellular soluble thiols were observed. The latter correlated closely with changes in cellular glutathione levels. ...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(95)05730-7
更新日期：1996-05-01 00:00:00
abstract：:The relationship of ischaemic heart disease (IHD) to seasonal and latitude variation has prompted speculation that exposure to the ultraviolet component of solar radiation may reduce IHD risk. This hypothesis was partially tested by exposing 14 post-myocardial infarction patients to a 6 week course of artificial whole...
journal_title：Atherosclerosis
pub_type： 临床试验,杂志文章
doi：10.1016/0021-9150(94)90142-2
更新日期：1994-05-01 00:00:00
abstract：:Using a simple, standardized denaturing gradient gel electrophoresis (DGGE) based mutation screening technique, a novel G-to-A mutation in the last base of the intron 12 splice acceptor site of the LDL receptor gene was found in 2 Danish families with familial hypercholesterolemia (FH). The mutation is shown to result...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/s0021-9150(96)05967-9
更新日期：1997-01-03 00:00:00
abstract：:We measured 381 genomewide markers and performed genetic linkage analyses in search of loci influencing coronary artery calcification (CAC), a measure of atherosclerosis determined by electron beam computed tomography, in 948 non-Hispanic white siblings (mean age [+/-standard deviation] = 59.6 +/- 9.9 years; 73.7% hyp...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2005.06.010
更新日期：2006-04-01 00:00:00
abstract：:Known cytochrome P450-dependent oxygenase inhibitor ketoconazole (5-50 microM) blocked the murine macrophage-mediated modification of human low density lipoprotein (LDL) as measured by production of thiobarbituric acid-reactive substance, stimulation of [125I]LDL degradation in a fresh set of macrophages and LDL elect...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(94)05456-s
更新日期：1995-04-07 00:00:00
abstract：BACKGROUND AND PURPOSE:It has not been fully determined whether non-high-density lipoprotein cholesterol (non-HDLC) levels are involved in vascular events, especially stroke, in general Asian populations. We evaluated the association between non-HDLC levels and the risk of type-specific cardiovascular disease in a pros...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2014.01.005
更新日期：2014-04-01 00:00:00
abstract：:Acute myocardial infarction is associated with profound alterations in the plasma lipoprotein profile. The mechanism of these alterations is not clear, and both cholesterol biosynthesis up- and downregulation could possibly be a consequence of acute myocardial infarction. We determined plasma lipids, lipoproteins, apo...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/s0021-9150(98)00242-1
更新日期：1999-02-01 00:00:00
