Abstract:
:Fission yeast phosphate homeostasis entails transcriptional induction of genes encoding phosphate-mobilizing proteins under conditions of phosphate starvation. Transcription factor Pho7, a member of the Zn2Cys6 family of fungal transcription regulators, is the central player in the starvation response. The DNA binding sites in the promoters of phosphate-responsive genes have not been defined, nor have any structure-function relationships been established for the Pho7 protein. Here we narrow this knowledge gap by (i) delineating an autonomous DNA-binding domain (DBD) within Pho7 that includes the Zn2Cys6 module, (ii) deploying recombinant Pho7 DBD in DNase I footprinting and electrophoretic mobility shift assays (EMSAs) to map the Pho7 recognition sites in the promoters of the phosphate-regulated pho1 and tgp1 genes to a 12-nucleotide sequence motif [5'-TCG(G/C)(A/T)xxTTxAA], (iii) independently identifying the same motif as a Pho7 recognition element via in silico analysis of available genome-wide ChIP-seq data, (iv) affirming that mutations in the two Pho7 recognition sites in the pho1 promoter efface pho1 expression in vivo, and (v) establishing that the zinc-binding cysteines and a pair of conserved arginines in the DBD are essential for Pho7 activity in vivoIMPORTANCE Fungi respond to phosphate starvation by inducing the transcription of a set of phosphate acquisition genes that comprise a phosphate regulon. Pho7, a member of the Zn2Cys6 family of fungal transcription regulators, is the central player in the phosphate starvation response in fission yeast. The present study identifies a 12-nucleotide Pho7 DNA binding motif [5'-TCG(G/C)(A/T)xxTTxAA] in the promoters of phosphate-regulated genes, pinpoints DNA and protein features important for Pho7 binding to DNA, and correlates them with Pho7-dependent gene expression in vivo The results highlight distinctive properties of Pho7 vis-a-vis other fungal zinc binuclear cluster transcription factors as well as the divergent cast of transcription factors deployed for phosphate homeostasis in fission yeast versus budding yeast.
journal_name
mBiojournal_title
mBioauthors
Schwer B,Sanchez AM,Garg A,Chatterjee D,Shuman Sdoi
10.1128/mBio.01218-17subject
Has Abstractpub_date
2017-08-15 00:00:00issue
4issn
2150-7511pii
mBio.01218-17journal_volume
8pub_type
杂志文章相关文献
mBio文献大全abstract::Epsilon toxin (ETX), a pore-forming toxin produced by type B and D strains of Clostridium perfringens, mediates severe enterotoxemia in livestock and possibly plays a role in human disease. During enterotoxemia, the nearly inactive ETX prototoxin is produced in the intestines but then must be activated by proteolytic ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01994-14
更新日期:2014-10-21 00:00:00
abstract:UNLABELLED:Methicillin-resistant Staphylococcus aureus (MRSA) strains are leading causes of hospital-acquired infections in the United States, and clonal cluster 5 (CC5) is the predominant lineage responsible for these infections. Since 2002, there have been 12 cases of vancomycin-resistant S. aureus (VRSA) infection i...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00112-12
更新日期:2012-05-22 00:00:00
abstract:UNLABELLED:Human noroviruses (HuNoVs) cause significant morbidity and mortality worldwide. However, despite substantial efforts, a small-animal model for HuNoV has not been described to date. Since "humanized" mice have been successfully used to study human-tropic pathogens in the past, we challenged BALB/c mice defici...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00450-13
更新日期:2013-07-16 00:00:00
abstract::Yellow fever virus (YFV) is an arthropod-borne flavivirus, infecting ~200,000 people worldwide annually and causing about 30,000 deaths. The live attenuated vaccine strain, YFV-17D, has significantly contributed in controlling the global burden of yellow fever worldwide. However, the viral and host contributions to YF...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00819-17
更新日期:2017-08-15 00:00:00
abstract::Mitochondrial function adapts to cellular demands and is affected by the ability of the organelle to undergo fusion and fission in response to physiological and nonphysiological cues. We previously showed that infection with the human bacterial pathogen Listeria monocytogenes elicits transient mitochondrial fission an...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03171-19
更新日期:2020-02-04 00:00:00
abstract::Here, we investigate a monoclonal antibody, Z2B3, isolated from an H7N9-infected patient, that exhibited cross-reactivity to both N9 (group 2) and a broad range of seasonal and avian N1 (group 1) proteins but lost activity to the N1 with the substitution K432E. This substitution exists in 99.25% of seasonal influenza ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02315-20
更新日期:2020-10-06 00:00:00
abstract:UNLABELLED:Despite long-term investment, influenza continues to be a significant worldwide problem. The cornerstone of protection remains vaccination, and approved vaccines seek to elicit a hemagglutination inhibition (HAI) titer of ≥1:40 as the primary correlate of protection. However, recent poor vaccine performance ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00417-16
更新日期:2016-04-19 00:00:00
abstract::Mucormycosis, caused by Rhizopus species, is a life-threatening fungal infection that occurs in patients immunocompromised by diabetic ketoacidosis (DKA), cytotoxic chemotherapy, immunosuppressive therapy, hematologic malignancies, or severe trauma. Inhaled Rhizopus spores cause pulmonary infections in patients with h...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01087-20
更新日期:2020-06-02 00:00:00
abstract::Nineteen Thermococcus kodakarensis strains have been constructed, each of which synthesizes a different His(6)-tagged protein known or predicted to be a component of the archaeal DNA replication machinery. Using the His(6)-tagged proteins, stable complexes assembled in vivo have been isolated directly from clarified c...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00221-10
更新日期:2010-10-26 00:00:00
abstract:UNLABELLED:Carbonate chimneys at the Lost City hydrothermal field are coated in biofilms dominated by a single phylotype of archaea known as Lost City Methanosarcinales. In this study, we have detected surprising physiological complexity in single-species biofilms, which is typically indicative of multispecies biofilm ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00127-11
更新日期:2011-09-01 00:00:00
abstract::All enterococci produce a complex polysaccharide called the enterococcal polysaccharide antigen (EPA). This polymer is required for normal cell growth and division and for resistance to cephalosporins and plays a critical role in host-pathogen interaction. The EPA contributes to host colonization and is essential for ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00277-20
更新日期:2020-04-28 00:00:00
abstract:UNLABELLED:Human cathepsin W (CtsW) is a cysteine protease, which was identified in a genome-wide RNA interference (RNAi) screen to be required for influenza A virus (IAV) replication. In this study, we show that reducing the levels of expression of CtsW reduces viral titers for different subtypes of IAV, and we map th...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00297-15
更新日期:2015-06-09 00:00:00
abstract:UNLABELLED:Fungal cell walls are predominantly composed of glucans, mannans, and chitin. Recognition of these glycans by the innate immune system is a critical component of host defenses against the mycoses. Complement, an important arm of innate immunity, plays a significant role in fungal pathogenesis, especially the...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00178-11
更新日期:2011-08-30 00:00:00
abstract:UNLABELLED:Mammalian lipopolysaccharide (LPS) binding proteins (LBPs) occur mainly in extracellular fluids and promote LPS delivery to specific host cell receptors. The function of LBPs has been studied principally in the context of host defense; the possible role of LBPs in nonpathogenic host-microbe interactions has ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01193-15
更新日期:2015-10-13 00:00:00
abstract:UNLABELLED:Clostridium perfringens enterotoxin (CPE) contributes to several important human gastrointestinal (GI) diseases. This toxin and its derivatives are also being explored for translational applications, i.e., cancer therapy or drug delivery. Some, but not all, members of the 24-member claudin (Cldn) family of m...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00594-12
更新日期:2013-01-15 00:00:00
abstract::Ocean viruses are ubiquitous and abundant and play important roles in global biogeochemical cycles by means of their mortality, horizontal gene transfer, and manipulation of host metabolism. However, the obstacles involved in linking viruses to their hosts in a high-throughput manner bottlenecks our ability to underst...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00373-12
更新日期:2012-10-30 00:00:00
abstract::Infection of host cells by the respiratory syncytial virus (RSV) is characterized by the formation of spherical cytoplasmic inclusion bodies (IBs). These structures, which concentrate all the proteins of the polymerase complex as well as some cellular proteins, were initially considered aggresomes formed by viral dead...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01202-20
更新日期:2020-09-22 00:00:00
abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of three subunits, namely, NF-YA, NF-YB, and NF-YC, which are conserved throughout evolution. In higher eukaryotes, NF-Y plays important roles in several cellular processes (development, cell cycle regulation, apoptosis, and response to growth, ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00737-19
更新日期:2019-06-18 00:00:00
abstract:UNLABELLED:The C-type lectin receptor Dectin-1 is expressed mainly on myeloid cells mediating the immune response targeting respiratory pathogens such as Aspergillus fumigatus and Mycobacterium tuberculosis. The pulmonary epithelium serves as an important interface for interactions between these pathogens and the respi...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01492-14
更新日期:2014-08-26 00:00:00
abstract:UNLABELLED:A 32-nucleotide (nt) RNA motif located at the 3' end of the transmissible gastroenteritis coronavirus (TGEV) genome was found to specifically interact with the host proteins glutamyl-prolyl-tRNA synthetase (EPRS) and arginyl-tRNA synthetase (RRS). This RNA motif has high homology in sequence and secondary st...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00105-15
更新日期:2015-03-10 00:00:00
abstract::Neisseria meningitidis (the meningococcus) is primarily a commensal of the human oropharynx that sporadically causes septicemia and meningitis. Meningococci adapt to diverse local host conditions differing in nutrient supply, like the nasopharynx, blood, and cerebrospinal fluid, by changing metabolism and protein repe...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02293-16
更新日期:2017-03-21 00:00:00
abstract::Biofilm-associated polymicrobial infections, particularly those involving fungi and bacteria, are responsible for significant morbidity and mortality and tend to be challenging to treat. Candida albicans and Staphylococcus aureus specifically are considered leading opportunistic fungal and bacterial pathogens, respect...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01365-16
更新日期:2016-10-11 00:00:00
abstract::Peptides that are synthesized independently of the ribosome in plants, fungi, and bacteria can have clinically relevant anticancer, antihemochromatosis, and antiviral activities, among many other. Despite their natural origin, discovering new natural products is challenging, and there is a need to expand the chemical ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01474-17
更新日期:2017-10-10 00:00:00
abstract::Bacillus anthracis is a Gram-positive bacillus that under conditions of environmental stress, such as low nutrients, can convert from a vegetative bacillus to a highly durable spore that enables long-term survival. The sporulation process is regulated by a sequential cascade of dedicated transcription factors but requ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02079-18
更新日期:2018-11-06 00:00:00
abstract::The major oxidative stress response in Streptomyces is controlled by the sigma factor SigR and its cognate antisigma factor RsrA, and SigR activity is tightly controlled through multiple mechanisms at both the transcriptional and posttranslational levels. Here we show that sigR has a highly unusual GTC start codon and...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00815-17
更新日期:2017-06-13 00:00:00
abstract::Misincorporation of uracil or spontaneous cytidine deamination is a common mutagenic insult to DNA. Herpesviruses encode a viral uracil-DNA glycosylase (vUNG) and a viral dUTPase (vDUT), each with enzymatic and nonenzymatic functions. However, the coordinated roles of these enzymatic activities in gammaherpesvirus pat...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01831-18
更新日期:2018-10-30 00:00:00
abstract::Peroxisomes are found in essentially all eukaryotic cells and have been described as important hubs in innate sensing and the induction of type III interferons upon viral infection. Nevertheless, it remains poorly investigated how viral pathogens modulate biogenesis or function of peroxisomes to evade innate sensing a...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00967-20
更新日期:2020-05-26 00:00:00
abstract:UNLABELLED:Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host. Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells. A number of pathogens, including C. albic...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01874-14
更新日期:2014-12-02 00:00:00
abstract::Like their cellular hosts, herpesviruses are subject to the regulatory impacts of chromatin assembled on their genomes. Upon infection, these viruses are assembled into domains of chromatin with heterochromatic signatures that suppress viral gene expression or euchromatic characteristics that promote gene expression. ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00098-16
更新日期:2016-02-16 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) resists nearly all β-lactam antibiotics that have a bactericidal activity. However, whether the empirically used β-lactams enhance MRSA pathogenicity in vivo remains unclear. In this study, we showed that a cluster of lipoprotein-like genes (lpl, sa2275 to sa2273 [sa2...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00880-19
更新日期:2019-06-11 00:00:00