Abstract:
:The present study aimed to investigate the effect of glutathione S-transferase A1 (GSTA1) on lung cancer cell viability, invasion and adhesion in the presence of nicotine in vitro. Furthermore, the effect of GSTA1 on the epithelial-mesenchymal transition (EMT), a process strongly associated with lung cancer metastasis, was examined. Human lung carcinoma A549 cells were treated with various concentrations of nicotine (0.01, 0.1, 1 and 10 µM) and levels of GSTA1 mRNA and protein were measured by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. To knock down GSTA1 expression, GSTA1-small interfering RNA was transfected into A549 cells. Cell viability, invasion and adhesion abilities were determined by MTT, Transwell-Matrigel invasion and cell adhesion assays, respectively. The expression of the epithelial cell markers E-cadherin and keratin, and the mesenchymal cell markers vimentin and N-cadherin in A549 cells were examined by western blot analysis. The current study indicated that the expression of GSTA1 was increased in A549 cells following nicotine treatment. GSTA1 suppression inhibited the viability, invasion and adhesion of lung cancer cells. In addition, the increase in lung cancer cell viability, invasion and adhesion by nicotine was suppressed following GSTA1 knockdown. Furthermore, GSTA1 affected the expression of EMT markers in nicotine-treated or untreated lung cancer cells. Thus the present study demonstrates that GSTA1 promotes lung cancer cell invasion and adhesion and mediates the effect of nicotine on lung cancer cell metastasis in vitro. Furthermore, the results demonstrated that GSTA1 exerts its effect on lung cancer cell metastasis by promoting the EMT.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Wang W,Liu F,Wang C,Wang C,Tang Y,Jiang Zdoi
10.3892/etm.2017.4663subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1783-1788issue
2eissn
1792-0981issn
1792-1015pii
ETM-0-0-4663journal_volume
14pub_type
杂志文章abstract::Gilbert syndrome (GS) is a hereditary unconjugated hyperbilirubinemia that results from mutations in the bilirubin uridine diphosphate-glucuronosyltransferase (UGT1A1) gene. To the best of our knowledge, there are currently no reports that focus on patients with systemic lupus erythematosus (SLE) coexisting with GS. T...
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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doi:10.3892/etm.2015.2432
更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
doi:10.3892/etm.2019.7972
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4584
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2016.3044
更新日期:2016-04-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9069
更新日期:2020-10-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.2063
更新日期:2015-01-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6166
更新日期:2018-07-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8193
更新日期:2020-01-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8008
更新日期:2019-12-01 00:00:00
abstract::Asthma is one of the diseases that demonstrates a wide range of variation in its clinical expression, in addition to an important heterogeneity in the pathophysiological mechanisms present in each case. The ever-increasing knowledge of the molecular signalling routes and the development of the Bio Immune(G)ene Medicin...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6019
更新日期:2018-06-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章,评审
doi:10.3892/etm.2020.8731
更新日期:2020-07-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4843
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5481
更新日期:2018-01-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9015
更新日期:2020-10-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6292
更新日期:2018-08-01 00:00:00
abstract::3-Hydroxy-3-methylglutaric aciduria (3-HMG, OMIN 246450) is a rare autosomal recessive metabolic disorder caused by a deficiency of 3-hydroxy-3-methylglutaryl-CoA lyase, a key enzyme in leucine metabolism and ketone body synthesis. Acute episodes of 3-HMG may be triggered by fasting or infection, and symptoms include ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2016.3243
更新日期:2016-06-01 00:00:00
abstract::The aim of this study was to investigate the surgical methods and efficacies for cervicothoracolumbar spinal schwannoma (CSS). A total of 52 patients who had undergone treatment for schwannoma were retrospectively analyzed. Two methods were employed for the surgical resection of the thoracic and lumbar schwannomas: Ty...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2015.2803
更新日期:2015-12-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2015.2261
更新日期:2015-04-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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更新日期:2018-04-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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更新日期:2020-03-01 00:00:00
abstract::Aortic valve (AoV) stenosis is the third most common cardiovascular disease. The pathogenesis of AoV stenosis is associated with an inflammatory process where MMPs serve important roles. The aim of the present study was to determine the association between matrix metalloproteinases (MMPs), tissue inhibitors of metallo...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.7254
更新日期:2019-04-01 00:00:00