Abstract：
:Colorectal cancer has a relatively low sensitivity to paclitaxel. The purpose of this study was to investigate the role of connexin 43 (Cx43), which is a structural component of gap junctional communication (GJC), in paclitaxel cytotoxicity in colorectal cancer cells. Three colorectal cancer cell lines (HCT106, HCT116 and LoVo) were transfected with Cx43 and used to examine paclitaxel cytotoxicity. A western blot assay was used to confirm Cx43 expression in transfected cell lines as well as the expression of several proteins that are associated with paclitaxel cytotoxicity. A parachute dye-coupling assay was used to measure GJC function. An MTT assay was used to analyze the viability of paclitaxel-treated cells. Cx43 expression level and GJC function were significantly upregulated by the transfection (P<0.05). The viability of transfected cells was significantly inhibited compared with that of untransfected cells when treated with paclitaxel (20 or 80 nM) at high culture density but not at low culture density (P<0.05). Cx43 transfection significantly increased the mitotic arrest, tubulin polymerization and apoptosis effects of paclitaxel (P<0.05). It was also found that paclitaxel had an inhibitory effect on GJC function after 12 h of treatment in LoVo cells (P<0.05). These results indicate that Cx43 may serve as a target of paclitaxel chemotherapy for colorectal cancer.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Wang S,Zhang S,Zhao Z,Zhang C,Yang X,Wang Ydoi
10.3892/etm.2017.4589subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1212-1218issue
2eissn
1792-0981issn
1792-1015pii
ETM-0-0-4589journal_volume
14pub_type
杂志文章abstract：:In the present study, the predictive value of glycosylated hemoglobin (HbA1c), microalbuminuria (24 h mAlb) and serum cystatin C (Cys-C) levels on the outcome of pregnancy in patients with gestational diabetes mellitus (GDM) was investigated. Samples of 144 females with GDM and 117 normal pregnant females as controls ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.8336
更新日期：2020-02-01 00:00:00
abstract：:The Chinese population are at an increased risk of autoimmune hepatitis (AIH). The aims of this study were to determine the demographic and clinical features of AIH in China. A total of 83 patients with AIH diagnosed by the revised scoring system were re-analyzed, and the clinical presentations among the different age...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2013.1363
更新日期：2014-01-01 00:00:00
abstract：:A previous study by our group showed that a 44-amino-acid fragment of pigment epithelium-derived factor (PEDF) facilitated corneal epithelial wound healing. In the present study this fragment was shortened to obtain peptides of 18, 20 and 29 amino acids in length, and their promoting effects on the healing of full-thi...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5134
更新日期：2017-11-01 00:00:00
abstract：:The aim of the present study was to determine the protective effects of resveratrol on a rat model of osteoporosis and examine the associated mechanisms of its action. Rats were randomized into the following groups: Control, osteoporosis, osteoporosis + low-dose resveratrol, osteoporosis + middle-dose resveratrol and ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5147
更新日期：2017-11-01 00:00:00
abstract：:The present study aimed to investigate the practicability and clinical value of applying laparotomy biliary lithotomy forceps to laparoscopic bile duct exploration (LCBDE) for the surgical treatment of incarcerated calculi. A total of 63 patients were diagnosed with cholecystolithiasis and choledocholithiasis. The pre...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3618
更新日期：2016-10-01 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti-cancer effects, in addition to protecting and supporting the immune system. The pres...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3152
更新日期：2016-05-01 00:00:00
abstract：:To investigate the pharmacological mechanism of Guizhi Fuling Wan (GFW) in the treatment of uterine fibroids, a network pharmacology approach was used. Information on GFW compounds was collected from traditional Chinese medicine (TCM) databases, and input into PharmMapper to identify the compound targets. Genes associ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5170
更新日期：2017-11-01 00:00:00
abstract：:We previously reported that cyclin E (CCNE1) amplification is strongly associated with resistance to treatment in serous ovarian cancer by high-resolution oligonucleotide copy number analysis. Dysregulation of cell cycle control has been implicated as the key event in human oncogenesis, and aberrant expression of G1-S...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2011.194
更新日期：2011-03-01 00:00:00
abstract：:The aim of the present study was to evaluate the thymuses of non-thymomatous myasthenia gravis (MG) patients by computed tomography (CT) for differentiating lymphoid follicular hyperplasia (LFH) thymus from normal/involuted thymus in order to assist surgeons in determining whether a non-thymomatous MG patient requires...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6948
更新日期：2019-01-01 00:00:00
abstract：:The present study aimed to investigate the expression of complement component 1, q subcomponent-binding protein (C1QBP) in colon cancer cells, and identify proteins that interact with C1QBP. Total proteins were extracted from both the tumor and normal tissues of 22 patients with colon cancer and analyzed using liquid ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4249
更新日期：2017-05-01 00:00:00
abstract：:Scarred uterus is caused by cesarean section surgery, and this condition affects further gestation and delivery in patients. Previous evidence suggested that resveratrol, a polyphenol compound, presents beneficial outcomes for patients with scarred uterus. Therefore, the aim of the present study was to analyze the eff...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6126
更新日期：2018-06-01 00:00:00
abstract：:The aim of the present study was to investigate the expression profiles of cyclooxygenase (COX)-2 and microRNA (miRNA)-143 in the tumor tissue and blood samples of patients with osteosarcoma, and their involvement in the disease pathogenesis. Tumor tissue and blood samples were obtained from 46 patients with osteosarc...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2015.2420
更新日期：2015-06-01 00:00:00
abstract：:The present study analyzed the ultrasonic features of papillary thyroid microcarcinoma (PTMC) and papillary thyroid non-microcarcinoma (non-PTMC) with the aim of improving the diagnostic value of ultrasonography. The ultrasonic features of 328 patients with papillary thyroid carcinoma (PTC), as confirmed by pathology,...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.1910
更新日期：2014-10-01 00:00:00
abstract：:Chronic obstructive pulmonary disease (COPD) has become a significant public health risk. Long non-coding RNAs (lncRNAs) have been identified as important factors involved in the proliferation, apoptosis and inflammatory cytokine expression of lung cells. Peripheral blood samples from 66 subjects (18 non-smokers, 24 s...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9492
更新日期：2021-01-01 00:00:00
abstract：:The present study aimed to compare the clinical outcome of patients with coronary artery disease (CAD) who underwent a revascularization using conventional coronary angiography or a physiologically guided revascularization with Fractional Flow Reserve (FFR). Furthermore, outcomes in FFR guided percutaneous coronary in...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.7156
更新日期：2019-03-01 00:00:00
abstract：:In the present study, we studied the influence of serum high-molecular weight adiponectin (HMWA) levels in patients with pregnancy-induced hypertension (PIH) on the occurrence of eclampsia in secondary pregnancy and its related mechanisms. In total, 130 patients who were diagnosed with PIH for the first time were sele...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5112
更新日期：2017-11-01 00:00:00
abstract：:The aim of the present study was to investigate whether modified Huangqi Chifeng decoction (MHCD) could be an effective treatment against Doxorubicin-induced nephrosis in rats and whether it regulates autophagy via the phosphoinositide-3 kinase/mammalian target of rapamycin (PI3K/mTOR) signaling pathway. A total of 40...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6492
更新日期：2018-09-01 00:00:00
abstract：:Numerous studies have demonstrated that neuronal cell death occurs via extrinsic (death receptors) and intrinsic (mitochondria) pathways. Radiation induces caspase activation fundamentally via the mitochondrial pathway. To investigate the role of caspase, a cell permeable pan-caspase inhibitor, z-VAD-fmk [N-benzyloxyc...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2013.1419
更新日期：2014-02-01 00:00:00
abstract：:The aim of the present study was to investigate the association between polymorphism in the serotonin transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR) in the promoter region of the 5-HTT gene and the pathogenesis of postpartum depression (PPD). Blood samples were collected from 120 female patients with PP...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.2043
更新日期：2015-01-01 00:00:00
abstract：:The aim of the present study was to investigate whether chronic administration of basic fibroblast growth factor (bFGF) following angioplasty in a dog model of atherosclerotic iliac stenosis may restore endothelium function and prevent restenosis (RS). In total, 40 dogs with atherosclerotic stenosis of the right iliac...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.1562
更新日期：2014-05-01 00:00:00
abstract：:The aim of the present study was to determine the prognostic value of peroxisome proliferator-activated receptor-γ (PPAR-γ) and phosphatase and tensin homologue deleted on chromosome ten (PTEN) for bladder cancer. Data were collected from The Cancer Genome Atlas (TCGA), a public database, and were analyzed to assess P...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.7879
更新日期：2019-10-01 00:00:00
abstract：:Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3-kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U251 cells in a logar...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.1756
更新日期：2014-08-01 00:00:00
abstract：:The aim of the present study was to investigate the prevalence rate of hepatitis C virus (HCV) infection in human immunodeficiency virus (HIV)-positive individuals and to study the infection status of HCV RNA in HIV-infected individuals who did not have anti-HCV antibodies in the Guangxi province of China, in order to...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4067
更新日期：2017-03-01 00:00:00
abstract：:The aim of the present study was to investigate the effect of Forkhead family transcription factor P3 (Foxp3) knockdown on the function of cluster of differentiation (CD)4+CD25+ regulatory T cell (Tregs) and the tumor growth of a hepatocellular carcinoma (HCC) mouse model. CD4+CD25+ Tregs and CD4+CD25- T cells were so...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5421
更新日期：2018-01-01 00:00:00
abstract：:Schisandrin B (SchB) is one of the primary active components of Schisandra chinensis (Turcz.) Baill., a traditional Chinese herb that has been used to treat insomnia for hundreds of years. Our previous studies revealed that SchB exerts sedative and hypnotic effects, increasing the content of γ-aminobutyric acid (GABA)...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9271
更新日期：2020-12-01 00:00:00
abstract：:The present study aimed to determine the diagnostic yield of OMOM capsule endoscopy for small bowel diseases in adults. A total of 89 patients, including 45 cases of obscure abdominal pain, 22 of chronic diarrhea, 18 of obscure gastrointestinal bleeding and 4 of obscure anemia were enrolled in the present study. The t...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.5864
更新日期：2018-04-01 00:00:00
abstract：:The aim of the present study was to investigate the effects of urantide on the expression status of C-reactive protein (CRP) and the inflammatory cytokines monocyte chemotactic protein (MCP)-1 and transforming growth factor (TGF)-β in the aortas of rats with atherosclerosis (AS), and to identify its underlying mechani...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.1654
更新日期：2014-06-01 00:00:00
abstract：:The present study aimed to explore the treatment effects of Taohongsiwu decoction on the liver fibrosis in a rat model, as few prior studies have investigated the anti-fibrotic effects of Taohongsiwu decoction. High-performance liquid chromatography was used to measure the concentration of ferulic acid and hydrosafflo...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4625
更新日期：2017-08-01 00:00:00
abstract：:The aim of this study was to evaluate the feasibility of transjugular intrahepatic portosystemic shunts using FLUENCY expanded polytetraf luoroethylene (PTFE)-covered stents and the effect on the patency rate. A total of 114 cirrhotic patients who were treated by transjugular intrahepatic portosystemic stent shunt (TI...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2012.776
更新日期：2013-01-01 00:00:00
abstract：:Chronic venous disorder (CVD) is a complex disease, that affects millions of people worldwide, and due to the fact that in its early stages is often overlooked by healthcare providers and ignored by the patient, the assessment of incidence and prevalence of CVD is difficult to be made. The aim of this project was to a...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.7059
更新日期：2019-02-01 00:00:00
