Abstract:
BACKGROUND:SLIT-ROBO families of proteins mediate axon pathfinding and their expression is not solely confined to nervous system. Aberrant expression of SLIT-ROBO genes was repeatedly shown in a wide variety of cancers, yet data about their collective behavior in hepatocellular carcinoma (HCC) is missing. Hence, we quantified SLIT-ROBO transcripts in HCC cell lines, and in normal and tumor tissues from liver. METHODS:Expression of SLIT-ROBO family members was quantified by real-time qRT-PCR in 14 HCC cell lines, 8 normal and 35 tumor tissues from the liver. ANOVA and Pearson's correlation analyses were performed in R environment, and different clinicopathological subgroups were pairwise compared in Minitab. Gene expression matrices of cell lines and tissues were analyzed by Mantel's association test. RESULTS:Genewise hierarchical clustering revealed two subgroups with coordinate expression pattern in both the HCC cell lines and tissues: ROBO1, ROBO2, SLIT1 in one cluster, and ROBO4, SLIT2, SLIT3 in the other, respectively. Moreover, SLIT-ROBO expression predicted AFP-dependent subgrouping of HCC cell lines, but not that of liver tissues. ROBO1 and ROBO2 were significantly up-regulated, whereas SLIT3 was significantly down-regulated in cell lines with high-AFP background. When compared to normal liver tissue, ROBO1 was found to be significantly overexpressed, while ROBO4 was down-regulated in HCC. We also observed that ROBO1 and SLIT2 differentiated histopathological subgroups of liver tissues depending on both tumor staging and differentiation status. However, ROBO4 could discriminate poorly differentiated HCC from other subgroups. CONCLUSION:The present study is the first in comprehensive and quantitative evaluation of SLIT-ROBO family gene expression in HCC, and suggests that the expression of SLIT-ROBO genes is regulated in hepatocarcinogenesis. Our results implicate that SLIT-ROBO transcription profile is bi-modular in nature, and that each module shows intrinsic variability. We also provide quantitative evidence for potential use of ROBO1, ROBO4 and SLIT2 for prediction of tumor stage and differentiation status.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Avci ME,Konu O,Yagci Tdoi
10.1186/1471-2407-8-392subject
Has Abstractpub_date
2008-12-29 00:00:00pages
392issn
1471-2407pii
1471-2407-8-392journal_volume
8pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:The Michigan Prevention Research Center, the University of Michigan Schools of Nursing, Public Health, and Medicine, and the Michigan Department of Community Health propose a multidisciplinary academic-clinical practice three-year project to increase breast cancer screening among young breast cancer survivor...
journal_title:BMC cancer
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2407-13-97
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:For gastric cancer patients, surgical resection with en-bloc lymphadenectomy is the cornerstone of curative treatment. Open gastrectomy has long been the preferred surgical approach worldwide. However, this procedure is associated with considerable morbidity. Several meta-analyses have shown an advantage in ...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-015-1551-z
更新日期:2015-07-29 00:00:00
abstract:BACKGROUND:Recently, a phase II clinical trial in hepatocellular carcinoma (HCC) has suggested that the combination of sorafenib and 5-fluorouracil (5-FU) is feasible and side effects are manageable. However, preclinical experimental data explaining the interaction mechanism(s) are lacking. Our objective is to investig...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-363
更新日期:2013-07-29 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are short, non-coding RNAs (~22 nt) that play important roles in the pathogenesis of human diseases by negatively regulating gene expression. Although miR-196a has been implicated in several other cancers, its role in non-small cell lung cancer (NSCLC) is unknown. The aim of the present st...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-348
更新日期:2012-08-09 00:00:00
abstract:BACKGROUND:To investigate the implications of prophylactic intraoperative Hyperthermic Intraperitoneal Chemotherapy (HIPEC) with D2 radical gastrectomy for locally advanced Gastric Cancer (AGC) in a randomized case control study. METHOD:Eighty consecutive patients with locally AGC were randomly separated into 2 groups...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6125-z
更新日期:2019-09-18 00:00:00
abstract:BACKGROUND:Serine-threonine kinases of the Raf family (A-Raf, B-Raf, C-Raf) are central players in cellular signal transduction, and thus often causally involved in the development of cancer when mutated or over-expressed. Therefore these proteins are potential targets for immunotherapy and a possible basis for vaccine...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-5-15
更新日期:2005-02-09 00:00:00
abstract:BACKGROUND:Cetuximab combined with chemotherapy is one of the first-line treatments of metastatic colorectal cancer. Although disease progression inevitably occurs, rechallenge and maintenance therapies using cetuximab-based regimens may be beneficial, particularly for patients with wild-type (WT) KRAS. CASE PRESENTAT...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3133-8
更新日期:2017-02-14 00:00:00
abstract:BACKGROUND:Gene-gene and gene-environment interactions involved in the metabolism of carcinogens may increase the risk of cancer. Our objective was to measure the interactions between common polymorphisms of P450 (CYP1A2, CYP1B1, CYP2E1), GSTM1 and T1, SULT1A1 and cigarette smoking in colorectal cancer (CRC). METHODS:...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-7-115
更新日期:2007-06-30 00:00:00
abstract:BACKGROUND:BRCA1 and BRCA2 mutations explain approximately one-fifth of the inherited susceptibility in high-risk Finnish hereditary breast and ovarian cancer (HBOC) families. EMSY is located in the breast cancer-associated chromosomal region 11q13. The EMSY gene encodes a BRCA2-interacting protein that has been implic...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3488-x
更新日期:2017-07-24 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene expression regulation and have been described as key regulators of carcinogenesis. Aberrant miRNA expression has been frequently reported in sporadic breast cancers, but few studies have focused on profiling hereditary breast ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-6640-y
更新日期:2020-02-22 00:00:00
abstract:BACKGROUND:We studied the cases of single and multiple HPV infection and analyzed the correlation with negative cases, and preneoplastic and neoplastic lesions of the uterine cervix with the aim of making a contribution to the prognostic factor under discussion. METHODS:Nine hundred nine women undergoing second level ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06946-7
更新日期:2020-05-19 00:00:00
abstract::Following publication of the original article [1], the authors reported the family name of the second author was incorrectly published. ...
journal_title:BMC cancer
pub_type: 已发布勘误
doi:10.1186/s12885-019-6048-8
更新日期:2019-08-29 00:00:00
abstract:BACKGROUND:Endometrial cancer, one of the most common malignant tumors, is a serious threat to women's health. Endometrial hyperplasia is a precursor of endometrial cancer. S100 calcium binding protein P (S100P) has been found to play important roles in many types of cancer. The present study aimed to investigate the e...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07350-x
更新日期:2020-09-03 00:00:00
abstract:BACKGROUND:Patients undergone kidney transplantation present higher risk of Urothelial Carcinoma (UC) development and represent a subgroup of special interest. To date, vinflunine is the only drug approved in Europe for the treatment of advanced UC after failure of platinum-based chemotherapy. However, to our knowledge...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2666-6
更新日期:2016-08-12 00:00:00
abstract:BACKGROUND:It is well known that osteopontin (OPN) plays an important role in tumor progression and that a high OPN expression level in several tumor entities correlates with poor prognosis in cancer patients. However, little is known about the prognostic relevance of the OPN mRNA splice variants. METHODS:We analyzed ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-131
更新日期:2012-04-02 00:00:00
abstract:BACKGROUND:Computational intelligence methods, including non-linear classification algorithms, can be used in medical research and practice as a decision making tool. This study aimed to evaluate the usefulness of artificial intelligence models for 5-year overall survival prediction in patients with cervical cancer tre...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3806-3
更新日期:2017-12-12 00:00:00
abstract:BACKGROUND:Patients with hepatocellular carcinoma waiting for liver transplantation are commonly treated with locoregional treatments, such as TACE and ablation, to prevent tumor progression and dropout and to improve long-term outcome after transplantation. We wanted to prospectively assess feasibility of systemic ant...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章
doi:10.1186/s12885-019-5760-8
更新日期:2019-06-11 00:00:00
abstract:BACKGROUND:Recent data in esophageal cancer suggests the variant allele of a single-nucleotide polymorphism (SNP) in XRCC1 may be associated with resistance to radiochemotherapy. However, this SNP has not been assessed in a histologically homogeneous clinical trial cohort that has been treated with a uniform approach. ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-176
更新日期:2011-05-17 00:00:00
abstract:BACKGROUND:Neoadjuvant systemic therapy (NST) is increasingly used in the treatment of breast cancer, yet it is clear that there is significant geographical variation in its use in the UK. This study aimed to examine stated practice across UK breast units, in terms of indications for use, radiological monitoring, patho...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07757-6
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:Salivary gland cancer (SGC) is one of the common malignancies of the head and neck area. It develops in the minor and major salivary glands and sometimes metastasizes to other organs, particularly to the lungs. Inhibitors of differentiation (Id) proteins are negative regulators of basic helix-loop-helix tran...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-141
更新日期:2013-03-22 00:00:00
abstract:BACKGROUND:Mucosal melanomas represent about 1% of all melanoma cases and classically have a worse prognosis than cutaneous melanomas. Due to the rarity of mucosal melanomas, only limited clinical studies with metastatic mucosal melanoma are available. Mucosal melanomas most commonly contain mutations in the gene CKIT,...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-516
更新日期:2014-07-16 00:00:00
abstract:BACKGROUND:Less than 1% of all breast cancer cases are found in men, who reportedly have inferior outcomes compared with matched women patients. Ethnic differences may also affect their prognosis. Here, we investigated overall survival (OS) and major prognostic factors for male breast cancer (MBC) in a cohort of Egypti...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-227
更新日期:2014-03-28 00:00:00
abstract:BACKGROUND:Abnormal glucose and lipids levels may impact survival after breast cancer (BC) diagnosis, but their association to other causes of mortality such as cardiovascular (CV) disease may result in a competing risk problem. METHODS:We assessed serum glucose, triglycerides (TG) and total cholesterol (TC) measured ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1928-z
更新日期:2015-11-17 00:00:00
abstract:BACKGROUND:To assess the efficacy of salvage radiotherapy (RT) for persistent or rising PSA after radical prostatectomy and to determine prognostic factors identifying patients who may benefit from salvage RT. METHODS:Between 1990 and 2003, 59 patients underwent RT for PSA recurrence after radical prostatectomy. Patie...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-26
更新日期:2008-01-29 00:00:00
abstract:BACKGROUND:Our previous cDNA array results indicated KIAA0101 as one of the differentially expressed genes in human hepatocellular carcinoma (HCC) as compared with non-cancerous liver. However, it is necessary to study its expression at protein level in HCC and its biological function for HCC cell growth. METHOD:Weste...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-6-109
更新日期:2006-04-29 00:00:00
abstract:BACKGROUND:It is well known that women suffer from negative consequences following breast cancer (BC) treatment and that their largely varying needs for rehabilitation are often unmet. Up to 43% of these women are at risk of developing chronic distress requiring complex interventions; however, how to early identify and...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06815-3
更新日期:2020-05-29 00:00:00
abstract:BACKGROUND:Kaiso has been identified as a new member of the POZ-zinc finger family of transcription factors that are implicated in development and cancer. Although controversy still exists, Kaiso is supposed to be involved in human cancer. However, there is limited information regarding the clinical significance of cyt...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-9-178
更新日期:2009-06-09 00:00:00
abstract:BACKGROUND:The feasibility of submandibular gland (SMG) preservation in oral squamous cell carcinoma (SCC) has occasionally been analyzed, but the differences in survival associated with the presence or absence of SMG preservation remain unknown. We aimed to prospectively evaluate the oncologic results of SMG preservat...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07534-5
更新日期:2020-10-27 00:00:00
abstract:BACKGROUND:Tumor recurrence and metastasis are the most common reason for treatment failure. Metastasis-associate in colon cancer-1 (MACC1) has been identified as a metastatic and prognostic biomarker for colorectal cancer and other solid tumors. Aldehyde dehydrogenase 1 (ALDH1), a marker of cancer stem cells, is also ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2903-z
更新日期:2016-11-10 00:00:00
abstract:BACKGROUND:Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer that is thought to be a precursor to most invasive and metastatic breast cancers. Understanding the mechanisms regulating the invasive transition of DCIS is critical in order to better understand how some types of DCIS become invasive. W...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1007-5
更新日期:2015-01-21 00:00:00