Abstract:
:IS1 is one of the smallest transposable elements found in bacteria (768 bp). It contains eight overlapping open-reading-frames (ORFs) greater than 50 codons, designated insA to insG and insB'. To determine which of the ORFs actually code for proteins involved in transposition, we have introduced amber codons into each ORF by site-directed mutagenesis which make neutral changes in the overlapping ORFs. Each mutant IS1 was then tested for its ability to mediate cointegrate formation in Su+ and Su- backgrounds. The mutant elements were also tested for trans-complementation in an IS1-free Salmonella background. Our results show that the products of the insA and insB genes are the only ones essential for cointegrate formation. We suggest that other ORFs may, however, encode accessory proteins.
journal_name
Geneticsjournal_title
Geneticsauthors
Jakowec M,Prentki P,Chandler M,Galas DJsubject
Has Abstractpub_date
1988-09-01 00:00:00pages
47-55issue
1eissn
0016-6731issn
1943-2631journal_volume
120pub_type
杂志文章相关文献
GENETICS文献大全abstract::An association was found earlier between the Ep(1) gene locus coding for an endopeptidase and the endosperm color gene Y(1) on chromosome 6 of Zea mays. By employing primary trisomics we have unequivocally placed the Ep(1) gene on chromosome 6, closely linked to the Y(1) locus. Additionally we describe new alleles at ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-08-01 00:00:00
abstract::A previously employed method that uses the composition of noncoding DNA as the basis of a test for selection between synonymous codons in plastid genes is reevaluated. The test requires the assumption that in the absence of selective differences between synonymous codons the composition of silent sites in coding seque...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Arabidopsis seedlings repair UV-induced DNA damage via light-dependent and independent pathways. The mechanism of the "dark repair" pathway is still unknown. To determine the number of genes required for dark repair and to investigate the substrate-specificity of this process we isolated mutants with enhanced sensitiv...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Transposable element activity is repressed in the germline in animals by PIWI-interacting RNAs (piRNAs), a class of small RNAs produced by genomic loci mostly composed of TE sequences. The mechanism of induction of piRNA production by these loci is still enigmatic. We have shown that, in Drosophila melanogaster, a clu...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.180307
更新日期:2015-12-01 00:00:00
abstract::We have amplified and sequenced the gene encoding Esterase-P (Est-P) in 10 strains of Drosophila melanogaster. Three premature termination codons occur in the coding region of the gene in two strains. This observation, together with other indirect evidence, leads us to propose that Est-P may be a pseudogene in D. mela...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::The conflicts between science and morals which still continue to arise despite the apparent hegemony of atheistic scientism over traditional Judeo-Christianity in the twentieth century reflect a basic contradiction in the metaphysical foundation of Western lives. As was set forth by Machiavelli, the contradiction inhe...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-09-01 00:00:00
abstract::We describe allelic variation at 28 gene loci in natural populations of D. willistoni. Seventy samples were studied from localities extending from Mexico and Florida, through Central America, the West Indies, and tropical South America, down to South Brazil. At least several hundred, and often several thousand, genome...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-01-01 00:00:00
abstract::Allozyme and inversion data from natural populations of Drosophila melanogaster from the eastern United States were analyzed to determine whether the clines at allozyme loci are due to nonrandom associations with common cosmopolitan inversions. All inversions show strong clines. Clines were large and significant for h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::One crossover point between a pair of homologous chromosomes in meiosis appears to interfere with occurrence of another in the neighborhood. It has been revealed that Drosophila and Neurospora, in spite of their large difference in the frequency of crossover points, show very similar plots of coincidence-a measure of ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::The Genetics Society of America's (GSA) Edward Novitski Prize is awarded to researchers who have solved challenging problems in genetics through experiments that demonstrate exceptional creativity and ingenuity. Job Dekker of the University of Massachusetts Medical School has been selected for the 2018 award in recogn...
journal_title:Genetics
pub_type: 传,历史文章,杂志文章
doi:10.1534/genetics.118.301641
更新日期:2018-11-01 00:00:00
abstract::Several multilocus models that incorporate both preferential mating and viability selection are studied. Specifically, a class of symmetric heterozygosity models are considered that assign individuals to phenotypic classes according to which loci are in heterozygous state regardless of the actual allelic content. Othe...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::This paper studies the properties of a new class of demographic parameters for age-structured populations and analyzes the effect of natural selection on these parameters. Two new demographic variables are introduced: the entropy of a population and the reproductive potential. The entropy of a population measures the ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::The Caenorhabditis elegans gene gld-1 is essential for oocyte development; in gld-1 (null) hermaphrodites, a tumor forms where oogenesis would normally occur. We use genetic epistasis analysis to demonstrate that tumor formation is dependent on the sexual fate of the germline. When the germline sex determination pathw...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::The hitchhiking model of population genetics predicts that an allele favored by Darwinian selection can replace haplotypes from the same locus previously established at a neutral mutation-drift equilibrium. This process, known as "selective sweep," was studied by comparing molecular variation between the polymorphic I...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::DNA double-strand breaks (DSBs) are repaired by homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ in yeast chromosomes has been observed only when HR is blocked, as in rad52 mutants or in the absence of a homologous repair template. We detected yKu70p-dependent imprecise NHEJ at a frequency of a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::In multigene families, variation among loci and alleles can contribute to trait evolution. We explored patterns of functional and genetic variation in six duplicated Arabidopsis thaliana trypsin inhibitor (ATTI) loci. We demonstrate significant variation in constitutive and herbivore-induced transcription among ATTI l...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.3.1419
更新日期:2004-03-01 00:00:00
abstract::I reexamine the use of isolation by distance models as a basis for the estimation of demographic parameters from measures of population subdivision. To that aim, I first provide results for values of F-statistics in one-dimensional models and coalescence times in two-dimensional models, and make more precise earlier r...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Population structure parameters commonly used for diploid species are reexamined for the particular case of tetrasomic inheritance (autotetraploid species). Recurrence equations that describe the evolution of identity probabilities for neutral genes in an "island model" of population structure are derived assuming tet...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Interactions between Drosophila C virus (DCV) and its natural host, Drosophila melanogaster, were investigated using 15 geographical population samples infected by intraabdominal inoculation. These strains derived from natural populations of D. melanogaster differed in susceptibility to the DCVc. One strain was "parti...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The existence of temporally stable frequency clines for In(2L)t in natural populations of Drosophila melanogaster suggests a role for selection in the maintenance of this polymorphism. We have collected nucleotide polymorphism data from the proximal breakpoint junction regions of In(2L)t to infer its evolutionary hist...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::In the yeast Saccharomyces cerevisiae ectopic recombination has been shown to occur at high frequencies for artificially created repeats, but at relatively low frequencies for a natural family of repeated sequences, the Ty family. Little is known about the mechanism(s) that prevent recombination between repeated seque...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::The genetics of phenotypic variation in inbred mice has for nearly a century provided a primary weapon in the medical research arsenal. A catalog of the genetic variation among inbred mouse strains, however, is required to enable powerful positional cloning and association techniques. A recent whole-genome resequencin...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.115014
更新日期:2010-07-01 00:00:00
abstract::Conspicuous differences in floral morphology are partly responsible for reproductive isolation between two sympatric species of monkeyflower because of their effect on visitation of the flowers by different pollinators. Mimulus lewisii flowers are visited primarily by bumblebees, whereas M. cardinalis flowers are visi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::During segmentation of vertebrate embryos, somites form in accordance with a periodic pattern established by the segmentation clock. In the zebrafish (Danio rerio), the segmentation clock includes six hairy/enhancer of split-related (her/hes) genes, five of which oscillate due to negative autofeedback. The nonoscillat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.163642
更新日期:2014-06-01 00:00:00
abstract::High-throughput sequencing and bioinformatic techniques have enhanced classical genetic analysis and are essential methods for geneticists. Tsukamoto and colleagues use numerous genomic and bioinformatics methods to explore the role of ribonucleoprotein complexes in regulating oocyte meiotic maturation, which is the t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301139
更新日期:2018-07-01 00:00:00
abstract::Fisher's geometric model was originally introduced to argue that complex adaptations must occur in small steps because of pleiotropic constraints. When supplemented with the assumption of additivity of mutational effects on phenotypic traits, it provides a simple mechanism for the emergence of genotypic epistasis from...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.199497
更新日期:2017-06-01 00:00:00
abstract::Mutations were induced in synchronous Chinese hamster cells by bromodeoxyuridine (BUdR) incorporated into cells for one-hour periods in the cell cycle. There was a very pronounced temporal dependence during the first half of the DNA synthesis period for the induction of damage leading to 6-thioguanine (6TG) and ouabai...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::A correlation between diversity levels and rates of recombination is predicted both by models of positive selection, such as hitchhiking associated with the rapid fixation of advantageous mutations, and by models of purifying selection against strongly deleterious mutations (commonly referred to as "background selecti...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::C. elegans spermatogenesis employs lysosome-related fibrous body-membranous organelles (FB-MOs) for transport of many cellular components. Previous work showed that spe-10 mutants contain FB-MOs that prematurely disassemble, resulting in defective transport of FB components into developing spermatids. Consequently, sp...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.047340
更新日期:2006-01-01 00:00:00
abstract::An analysis of the effects of spontaneous mutations affecting age-specific mortality was conducted using 29 lines of Drosophila melanogaster that had accumulated spontaneous mutations for 19 generations. Divergence among the lines was used to estimate the mutational variance for weekly mortality rates and the covarian...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00