Abstract:
:Semaphorin 3A (SEMA3A), a secretory protein, is a founding member of the semaphorin family and functions in both the biological behavior of tumor cells and the modulation of tumor-associated macrophages. However, the role of SEMA3A in hepatocellular carcinoma (HCC) is still not well established. In the present study, we investigated the expression levels of SEMA3A in 80 HCC tissues and cell lines, using RT-qPCR, western blotting and immunohistochemistry. Expression profile analysis revealed that SEMA3A was significantly overexpressed in human HCC patients and positively correlated with the metastatic potential of HCC cells. Lentiviral transfection into PLC/PRF/5 and HCCLM3 cells was performed to stably upregulate and downregulate the expression of SEMA3A in HCC cells. Cell Counting Kit-8 (CCK-8), wound-healing and invasion assays revealed that SEMA3A promoted the proliferation and migration of HCC cells in vitro. Proteome profiler antibody microarray analysis revealed that overexpression of SEMA3A in HCC cells induced a significant increase in the expression levels of gelsolin-like capping protein (CapG), galectin-3, enolase 2 and epithelial cell adhesion molecule (EpCAM). Furthermore, the upregulation of SEMA3A in HCC cells promoted tumor growth and progression in an HCC mouse model. These results indicate that SEMA3A enhances CapG, galectin-3, enolase 2 and EpCAM expression to promote HCC progression and is a potential therapeutic target for HCC.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Li X,Chen Q,Yin D,Shi S,Yu L,Zhou S,Chen E,Zhou Z,Shi Y,Fan J,Zhou J,Dai Zdoi
10.3892/or.2017.5616subject
Has Abstractpub_date
2017-06-01 00:00:00pages
3313-3320issue
6eissn
1021-335Xissn
1791-2431journal_volume
37pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Garlic and its constituents are reported to have a preventive effect against colorectal cancer in animal models. Aged garlic extract (AGE), which is produced by natural extraction from fresh garlic for more than 10 months in aqueous ethanol, also has reputed chemopreventive effects on colon carcinogenesis, but has nev...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3705
更新日期:2015-03-01 00:00:00
abstract::The majority of patients with hormone receptor-positive metastatic breast cancer die from disease progression despite different types of anti-hormonal treatments. Preclinical studies have indicated that resistance to anti-hormonal therapies may be the result of an activated NF-κB si...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1562
更新日期:2012-03-01 00:00:00
abstract::A growing number of studies suggest that the hepatitis B virus X protein (HBx) enhances the protein stability of the hypoxia-inducible factor-1α (HIF-1α). However, the relationship between hepatitis B virus (HBV), HBx and hypoxia-inducible factor-2α (HIF-2α) has not yet been fully elucidated. Immunohistochemical analy...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4480
更新日期:2016-03-01 00:00:00
abstract::The Houttuynia cordata Thunb (HCT) extract has been used as a traditional Chinese herb medicine and as well as an effective drug for treating allergic inflammation for thousands of years. In this study, we investigated the anti-cancer activity of HCT and its molecular mechanisms in the human colon adenocarcinoma cell ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000535
更新日期:2009-11-01 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) are the most common genetic variation in mammalian cells with prognostic potential. Anillin‑actin binding protein (ANLN) has been identified as being involved in PI3K/PTEN signaling, which is critical in cell life/death control, and kinase insert domain receptor (KDR) encodes a k...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7332
更新日期:2019-12-01 00:00:00
abstract::Burkitt's lymphoma (BL) is a fast growing cancer of the human lymphatic system, and an extremely invasive B-cell non-Hodgkin's lymphoma. We explored the mechanism of apoptosis in Raji cells associated with the post-transcriptional regulation factors. To confirm that the predicted microRNA-520a (miR-520a) is matched wi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4975
更新日期:2016-09-01 00:00:00
abstract::Mice are particularly susceptible to carcinogens when exposure starts early in life. We evaluated the expression of stem cell antigen-1 (Sca-1) gene in the lung of variously aged CD-1 mice, either untreated or exposed to environmental cigarette smoke (ECS) and/or to a light source. Sca-1 expression progressively decre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000458
更新日期:2009-09-01 00:00:00
abstract::Numerous human chemoprevention studies have demonstrated that non-steroidal anti-inflammatory drugs (NSAIDs) possess chemopreventive effects against a variety of malignant tumors. However, there have been many clinical studies on aspirin, but not ibuprofen, even though ibuprofen is one of the most clinically and safel...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2713
更新日期:2013-11-01 00:00:00
abstract::Galla Rhois is a commonly used medicine in East Asia for the treatment of several diseases. However, the effects of Galla Rhois on the metastasis of colorectal cancer (CRC) and the underlying molecular mechanisms have not been studied. We investigated the anti‑metastatic properties of Galla Rhois water extract (GRWE) ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6812
更新日期:2019-01-01 00:00:00
abstract::Despite the established importance of angiogenesis in the pathogenesis of solid tumours, there is still no consensus on how this is best measured or which method is the most appropriate in the determination of prognosis. Here we review the pros and cons of current methods of assessing angiogenesis, both clinical and i...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.13.5.931
更新日期:2005-05-01 00:00:00
abstract::Highly malignant gliomas are characterized by pronounced intra‑ and intertumoral heterogeneity. On the genetic level, this heterogeneity may be caused by spontaneous mutation events, but recent studies have reported distinct mutational signatures that may be caused by an enzyme family with cytidine desaminase activity...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6698
更新日期:2018-11-01 00:00:00
abstract::To study the role of the chemokine receptor CCR7 in the metastatic process, a murine CCR7 gene was transduced in two mammary cancer cell lines with different origins and molecular features; TS/A, derived from a spontaneous mammary cancer of BALB/c strain, and N202.1A, derived from a HER-2/neu transgenic mammary cancer...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-08-01 00:00:00
abstract::Mammary stem cells (MSCs) are the progenitor population for human breast epithelia. MSCs give rise during mammary gland development to estrogen receptor (ER)-negative basal cells and the ER- luminal progenitor (LP) population which maintains ER+ and ER- luminal cells. The MSC population is expanded and tumorigenic in ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4003
更新日期:2015-07-01 00:00:00
abstract::Muscle atrophy F-Box (MAFbx)/atrogin-1 and muscle ring-finger-1 (MuRF-1) have been identified as two muscle-specific E3 ubiquitin ligases that are highly expressed in skeletal muscle during muscle atrophy. However, the role of muscle-specific E3 ubiquitin ligases during the process of muscle atrophy of cancer cachexia...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3845
更新日期:2015-05-01 00:00:00
abstract::Breast cancer is the most common malignancy in women, and metastasis is the leading cause of death in breast cancer patients. Previous studies have shown that epithelial‑mesenchymal transition (EMT) is involved in the metastasis of breast cancer, but the metabolic reprogramming and regulation mechanisms involved in th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7661
更新日期:2020-09-01 00:00:00
abstract::Progestin and adipoQ receptor family member III (PAQR3) is a regulator that negatively modulates the Ras/Raf/MEK/ERK signaling cascade and the GPCR Gβγ subunit signaling pathway. The role of PAQR3 in hepatocellular carcinoma (HCC) has not been elucidated. The present study investigated the expression of PAQR3 and its ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3532
更新日期:2014-12-01 00:00:00
abstract::The blood‑brain barrier (BBB) constitutes an efficient organization of tight junctions that limits the delivery of tumor to the brain. The principal tight junction protein in BBB is claudin‑5 (CLDN5), but its mechanism of action remains largely unknown. Long non‑coding RNAs (lncRNAs) are aberrantly expressed in many c...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6208
更新日期:2018-03-01 00:00:00
abstract::It has been revealed that the induction of epithelial‑mesenchymal transition (EMT) is associated with drug resistance, leading to tumor recurrence and metastasis. Recent studies have shown that chemotherapeutic agents, besides their therapeutic effects, can induce EMT and enhance invasive and metastatic properties of ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5726
更新日期:2017-08-01 00:00:00
abstract::The usefulness of diagnostic ultrasound in gene transfer was investigated. The hepatocellular carcinoma cell lines PRL/PRF/5 and Hep3B, and the pancreatic carcinoma Panc-1 cells were transfected with lipofectin or irradiated with a linear probe with a frequency of 8 MHz at a mechani...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1643
更新日期:2012-05-01 00:00:00
abstract::Non‑small cell lung cancer (NSCLC) is the most common type of lung cancer, and numerous oncogenes are associated with this disease. Oxysterol‑binding protein‑related protein 8 (ORP8) is essential for cell growth, migration and the modulation of mitochondrial respiration and morphology. However, the underlying role of ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7517
更新日期:2020-05-01 00:00:00
abstract::CD44 has diverse functions in cell-cell and cell-matrix interactions and may be a determinant of metastatic and invasive behaviour in carcinomas. CD44 variant 6 (CD44v6) has been postulated to be involved in both carcinogenesis and tumor progression. Therefore, we have examined CD44v6 expression in tumors from 57 pati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.11.1.41
更新日期:2004-01-01 00:00:00
abstract::Prostate cancer is a leading cause of cancer-related death among men. Early diagnosis and treatment are successful against prostate cancer, yet the clinical treatment of advanced prostate cancer remains a challenge. Gemcitabine is used to treat a broad spectrum of solid tumors; however, the clinical response of prosta...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3696
更新日期:2015-03-01 00:00:00
abstract::This study was conducted to assess protective effect of an antioxidant protein, sericin, on tumor promotion in the 7,12-dimethylbenz (alpha) anthracene (DMBA)-initiated and 12-O-tetradecanoylphorbol 13-acetate (TPA)-promoted mouse skin tumorigenesis model. In experiment 1, sericin was applied topically to DMBA-initiat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::More than 80% of hepatocellular carcinoma (HCC) arises in HBV based liver cirrhotic patients, suggesting that patients with cirrhosis form the main risk group of HCC. α fetoprotein (AFP) has poor sensitivity for the detection of AFP-negative and/or small tumors in HCC patients. Screening serum markers, along with HCC ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000982
更新日期:2010-11-01 00:00:00
abstract::Ovarian cancer (OC) is one of the most lethal gynecological malignancies in the world. The aim of the present study was to examine the role of microRNA (miR)-134-3p in OC. Reverse transcription-quantitative PCR was used to measure the expression levels of miR-134-3p. Cell Counting Kit-8, TUNEL, flow cytometric and col...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7844
更新日期:2021-01-01 00:00:00
abstract::To elucidate the potential etiological role of RUNX3 in the development of salivary gland adenoid cystic carcinoma (ACC), we analyzed the methylation status of RUNX3 in a series of 114 ACC tissues and 3 ACC cell lines. Results showed that the methylated rate of RUNX3 was 50.9 and 3.5% in the 114 ACC samples and the co...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1282
更新日期:2011-08-01 00:00:00
abstract::EGFR and KRAS mutations are the two most common driver mutations in non-small cell lung cancer (NSCLC). Molecular target-based therapy using small molecules such as gefitinib has been used for inhibiting EGFR with good initial responses; however, drug resistance is common when using a mono-targeting strategy. At prese...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4770
更新日期:2016-07-01 00:00:00
abstract::Cyclin Y (CCNY) is a newly identified PFTK1 interacting protein and has been found to be associated with the proliferation and tumorigenesis of human non-small cell lung cancer. In the present study, we analyzed the expression levels of CCNY in 65 cases of breast cancer (BC) tissues and in four BC cell lines, BT-474, ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5126
更新日期:2016-11-01 00:00:00
abstract::Reactive oxygen species (ROS) can drive the de‑differentiation of tumor cells leading to the process of epithelial-to-mesenchymal transition (EMT) to enhance invasion and metastasis. The invasive and metastatic phenotype of malignant cells is often linked to loss of E-cadherin expression, a hallmark of EMT. Recent stu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3448
更新日期:2014-11-01 00:00:00
abstract::As part of the efforts to drug repurposing, some HIV drugs have recently been identified to exert anticancer effects. Selected nucleoside analogues of nucleosidic reverse-transcriptase inhibitors (NRTIs) have been shown to interfere with RNA transcription of HI viruses as well as with the replication of DNA in cancer ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5243
更新日期:2017-01-01 00:00:00