Abstract:
:The Houttuynia cordata Thunb (HCT) extract has been used as a traditional Chinese herb medicine and as well as an effective drug for treating allergic inflammation for thousands of years. In this study, we investigated the anti-cancer activity of HCT and its molecular mechanisms in the human colon adenocarcinoma cell line HT-29. HCT inhibited HT-29 cell viability in a dose- and time-dependent manner by MTT assay. Treatment with 450 microg/ml of HCT for 48 and 72 h led to DNA damage and apoptosis by DAPI staining and comet assay. HCT increased reactive oxygen species production and decreased the levels of mitochondria membrane potential (MMP) in HT-29 cells by flow cytometry analysis. HCT caused the release of cytochrome c, Apaf-1, pro-caspase-9 and AIF from mitochondria via a decrease of the MMP. The decrease of MMP was then associated with a decrease in the ratio of Bax/Bcl-2 and activation of caspase-9 and -3 by Western blotting and caspase activity assay. Caspase-9 and -3 inhibitors almost completely suppressed HCT-induced caspase-9 and -3 activities. Our results demonstrated that the HCT-induced apoptosis in human colon adenocarcinoma cell line HT-29 might be related to a mitochondrial-dependent pathway.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Tang YJ,Yang JS,Lin CF,Shyu WC,Tsuzuki M,Lu CC,Chen YF,Lai KCdoi
10.3892/or_00000535subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1051-6issue
5eissn
1021-335Xissn
1791-2431journal_volume
22pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Recent studies have shown that miRNAs have potent abilities to activate gene expression by targeting promoter elements, a phenomenon known as RNA activation (RNAa). In the present study, we identified a new endogenous miR-3619-5p which was decreased in prostate cancer tissues and cells compared to corresponding normal...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5250
更新日期:2017-01-01 00:00:00
abstract::In humans, four tropomyosin genes (TPM1, TPM2, TPM3, and TPM4) are known to produce a multitude of isoforms via alternate splicing and/or using alternate promoters. Expression of tropomyosin has been shown to be modulated at both the transcription and the translational levels. Tropomyosins are known to make up some of...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4732
更新日期:2016-06-01 00:00:00
abstract::Trans-3,4',5-trihydroxystilbene (resveratrol) is a grape polyphenol present in various plants, food products, red wine and grapes. Resveratrol has anti-inflammatory, anticarcinogenic, anti-oxidant and anti-aging properties. Matrix metalloproteinases (MMPs) are key enzymes involved i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2151
更新日期:2013-02-01 00:00:00
abstract::Tubulin is a major component of microtubules. Class III beta-tubulin (beta III) is a neuron-associated beta-tubulin isotype and expressed in the normal central and peripheral nervous systems. According to a previous study, beta III is not expressed in normal skin and squamous cell carcinoma. However, its expression ha...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000494
更新日期:2009-10-01 00:00:00
abstract::Protocadherin-10 (PCDH10), a member of non-clustered protocadherin family which plays important roles in calcium-dependent cell-cell signal transduction and adhesion. PCDH10 functions as a tumor suppressor gene and its expression is downregulated by promoter methylation in many malignances. In the present study, we ex...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4793
更新日期:2016-07-01 00:00:00
abstract::Oral lichen planus (OLP) is a chronic inflammatory disease that may transform to oral squamous cell carcinoma (OSCC), while its carcinogenesis mechanisms are not entirely clear. This study was designed to identify the important genes involved in the malignant transformation of OLP to OSCC. After RNA-sequencing, the di...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5487
更新日期:2017-04-01 00:00:00
abstract::We compared effects of a high fat diet and a carcinogen on cellular elements of the spleen and mammary gland tumors in rats. Animals were fed a 15% olive-oil diet and a group of them were exposed to a carcinogen, dimethylbenz(a)anthacene (DMBA), in two doses of 10 mg/rat. Results of the experiments were evaluated afte...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.2.435
更新日期:2001-03-01 00:00:00
abstract::An arsenical compound, As2O3 has been reported to be effective for treating acute leukemia and induce apoptosis in many different tumor cell types. In this study we designed a novel arsenical compound, As4O6, and compared its ability to induce cell growth inhibition as well as gene expression profiles along with As2O3...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Fluoroquinolones affect the proliferation and apoptotic cell death of several human malignancies. Therefore, we investigated whether new 6-aminoquinolone derivatives, initially synthesized as anti-HIV agents, could affect the proliferation and apoptotic cell death of human prostate cancer cell lines. PC3 and LNCaP cel...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Tumor invasion and migration are major causes of mortality in patients with cervical carcinoma. Tumors under hypoxic conditions are more invasive and have a higher metastasic activity. Lysyl oxidase (LOX) is a hypoxia-responsive gene. LOX has been shown to be essential for hypoxia-induced metastasis in breast cancer. ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2146
更新日期:2013-02-01 00:00:00
abstract::Emodin is an active ingredient derived from root and rhizome of Rheum palmatum L and many studies have reported that it exhibits anticancer effects in a number of human tumors. However, there is little information demonstrating the possible effects of emodin on the proliferation and apoptosis of hepatocellular carcino...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4861
更新日期:2016-08-01 00:00:00
abstract::Extracellular matrix remodeling crucial to tumorigenesis involves proteolytic enzymes, primarily matrix metalloproteinases (MMPs). MMP production is stimulated by multiple factors, including the extracellular matrix metallo-proteinase inducer EMMPRIN/CD147. Overexpression of EMMPRIN...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1474
更新日期:2012-01-01 00:00:00
abstract::Frequent frameshift mutations in the DNA mismatch repair genes hMSH3, and hMSH6, have been reported in colorectal and endometrial cancers with microsatellite instability, however, it is unclear whether they are similarly altered in ovarian endometrioid carcinoma. In this study, we examined frequency of frameshift muta...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::The aim of this study was to look for a new medicine in diagnosing and treating of glioblastoma. Radioiodine-labeled anti-epidermal growth factor receptor (EGFR) binding nanoparticles were constructed. In vitro cell-binding assays were confirmed by confocal microscopy and flow cytometry. Cell cytotoxicity assays were ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5937
更新日期:2017-11-01 00:00:00
abstract::Aquaporins (AQPs) are a family of small, integral membrane proteins that have been shown to play an important role in tumor development and metastasis. Several studies have demonstrated that expression of AQP3 contributes to the enhanced migration of epithelial cells and is related to differentiation, metastasis and v...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2144
更新日期:2013-02-01 00:00:00
abstract::We have previously reported a high prevalence of hypothyroidism among patients with uveal melanoma. The objectives of the present study were to determine if a similar pattern of thyroid pathology exists among patients with cutaneous melanoma as well. To address this question, the medical records of all patients regist...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::Recently, gene fusions between the androgen responsive gene TMPRSS2 and members of the ETS-family of DNA-binding transcription factor genes were found in prostate cancer. Recurrent fusions were identified between the 5'-noncoding region of TMPRSS2 and ERG, or less frequently ETV1 or ETV4, resulting in overexpression o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significa...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4500
更新日期:2016-03-01 00:00:00
abstract::Human Pinx1 protein, associated with shelterin proteins, is widely revealed as a haploinsufficient tumor suppressor. Growing evidence has manifested the deregulation of PinX1 in distinct cancers. Nonetheless, the loss status of PinX1 and its diagnostic, prognostic and clinicopathological significance in Basal-like bre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5696
更新日期:2017-07-01 00:00:00
abstract::Ubiquitin specific protease 22 (USP22) is an oncogene that is upregulated in many cancer types, and aberrant expression of USP22 correlates with clinical outcome. However, its potential functional impact in epithelial ovarian cancer (EOC) has not been determined. Here, we report that USP22 was upregulated in EOC speci...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3580
更新日期:2015-01-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases, capable of degrading all the molecular components of extracellular matrix. MMPs have been shown to play critical roles in tumor cell invasion and metastasis. We verified the activity of MMPs in the sera ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1864
更新日期:2012-09-01 00:00:00
abstract::Elimination of leukoplakia is one of the strategies for prevention of oral cancer. However, the efficacy of reducing malignant transformation for the treated leukoplakia has rarely been reported despite a number of studies addressing its malignant transformation to oral cancer after intervention. The obstacle to do so...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:
更新日期:2003-11-01 00:00:00
abstract::The objective of the study was to investigate the dynamic changes of cortisol and soluble TNF-alpha p-55 and p-75 receptors in serum of advanced cancer patients during 24 h. The examined group consisted of 42 patients suffering from advanced gastrointestinal neoplasms (colorectal, gastric and pancreatic cancer). Serum...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.1.207
更新日期:2001-01-01 00:00:00
abstract::The estrogen receptor (ER) expression and HER2 amplification are important factors in determining the prognosis and therapy of breast cancer. Interactions between the two signaling pathways for example resulted in ERalpha-dependent regulation of HER2 expression in breast cancer cells. In this study, we investigated to...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-03-01 00:00:00
abstract::Increasing evidence suggests that microRNAs are involved in human carcinogenesis as tumor suppressors or oncogenes. A growing number of reports has shown that an interest has been sparked in aberrant microRNA expression and how they can be used to treat human diseases, including cancer. However, their precise biologic...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000834
更新日期:2010-07-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most commonly diagnosed cancer worldwide. It has poor clinical outcome due to intrinsic or acquired drug resistance. Deregulation of both apoptosis and autophagy contributes to chemotherapy resistance and disease progression. A new member of the inhibitors of ...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2019.7082
更新日期:2019-05-01 00:00:00
abstract::The purpose of this study was to define gene expression profile changes in colorectal tumors in order to identify target genes involved in neoplastic progression. cDNA microarray analysis was used to detect differences in gene expression profiles between colon tumor samples obtained from 20 patients in different tumor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::Breast cancer is one of the most malignant diseases world-wide and it ranks the first among female cancers. Masses of intrinsic and extrinsic factors, especially the inflammatory factors can lead to breast cancer. Aberrant activation and accumulation of key molecules can lead to inflammation associated carcinogenesis....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5393
更新日期:2017-03-01 00:00:00
abstract::The aim of the present study was to identify potential prognostic microRNA (miRNA) biomarkers for colon adenocarcinoma (COAD) prognostic prediction using the dataset of The Cancer Genome Atlas (TCGA). The genome‑wide miRNA sequencing dataset and corresponding COAD clinical information were downloaded from TCGA. Progno...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6607
更新日期:2018-10-01 00:00:00
abstract::We investigated a phase III randomized trial to compare efficacy and tolerance of CDDP + 5-FU to CDDP + VP16, both given intravenously in patients with unresectable advanced head and neck cancer. The 197 eligible patients were paired off successively on the basis of tumor sites and UICC stage. Comparisons were made th...
journal_title:Oncology reports
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2005-09-01 00:00:00