Abstract:
:Chromosomal locations and amounts of 5-methylcytosine-rich chromosome regions were detected in the karyotypes of 13 bird species by indirect immunofluorescence using a monoclonal anti-5-methylcytosine antibody. These species belong to 7 orders and 10 families of modern (Neognathae) and primitive (Palaeognathae) birds and are characterized by macro- and microchromosomes as well as ZW sex chromosomes. In all 13 species, the hypermethylated chromosome segments are confined to constitutive heterochromatin. The chromosomal locations of hypermethylated DNA regions in the karyotypes are constant and species-specific. There is no general rule with regard to the distribution of these hypermethylated chromosome regions in the genomes of birds. In most instances, hypermethylated segments are located in the centromeric regions of chromosomes, but in the sex chromosomes, these can also be found in telomeric and interstitial postitions. In most of the species studied, the centromeric heterochromatin in many, if not all, of the microchromosomes is hypermethylated. However, in one species, the only detectable hypermethylated heterochromatic regions are located in one pair of macroautosomes and in the Z sex chromosome, but none of the microchromosomes contains visible quantities of 5-methylcytosine. The analysis of 5-methylcytosine-rich chromosome regions can be very helpful for the comparative cytogenetics of closely related species or subspecies. It also reflects the dynamic evolutionary process operating in the highly repetitive DNA of eukaryotic chromosomes.
journal_name
Cytogenet Genome Resjournal_title
Cytogenetic and genome researchauthors
Schmid M,Steinlein Cdoi
10.1159/000464268subject
Has Abstractpub_date
2017-01-01 00:00:00pages
216-227issue
4eissn
1424-8581issn
1424-859Xpii
000464268journal_volume
151pub_type
杂志文章abstract::The common fragile sites (CFSs) are large regions of profound genomic instability found in all individuals. A number of the CFSs have been found to span genes that extend over large genomic regions (>700 kb). The expression of these genes is frequently abrogated in a number of different cancers and several of them hav...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000138886
更新日期:2008-01-01 00:00:00
abstract::In rye each B chromosome (B) represents 5.5% of the diploid A genome. Rye Bs have several nuclear to whole plant effects although they seem to bear no genes except for the ones that lead to their maintenance within a population. In this context, and considering that rye Bs are enriched in repetitive non-coding regions...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000079306
更新日期:2004-01-01 00:00:00
abstract::As chromatin structures, telomeres undergo epigenetic regulation of their maintenance and function. In plants, these processes are likely of a higher complexity than in animals or yeasts, as exemplified by methylation of cytosines in plant telomeric DNA or reversible developmental regulation of plant telomerase. We hi...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000360775
更新日期:2014-01-01 00:00:00
abstract::Human papillomaviruses (HPV) are responsible for the development of almost all cervical cancers. HPV is also found in 85% of anal cancer and in 50% of penile, vulvar, and vaginal cancers, and they are increasingly found in a subset of head and neck cancers, i.e., oropharyngeal squamous cell carcinomas (OPSCC). The mod...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000458166
更新日期:2016-01-01 00:00:00
abstract::Hordeum chilense Roem. et Schult. has a number of characteristics interesting for breeding: high crossability with other Triticeae, resistance to biotic and abiotic stresses and high variability for quality traits such as endosperm storage proteins or carotenoid content. xTritordeum, the amphiploids between H. chilens...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000082423
更新日期:2005-01-01 00:00:00
abstract::Chromosomal mutagen sensitivity is a common feature of cells from patients with different kinds of cancer. A portion of breast cancer patients also shows an elevated sensitivity to the induction of chromosome damage in cells exposed to ionizing radiation or chemical mutagens. Segregation analysis in families of patien...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000077511
更新日期:2004-01-01 00:00:00
abstract::To obtain an estimate of the variation in common fragile sites (CFSs) among individuals, aphidicolin (APC)-induced chromosomal breakage data were analyzed for 20 karyotypically normal adult humans. As it is specifically designed to meet the analytical requirements for considering fragile sites as presence/absence char...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000073411
更新日期:2003-01-01 00:00:00
abstract::Osteosarcomas are primary tumors of bone that most often develop in adolescents. They are characterized by complex genomic changes including amplifications, deletions, and translocations. The chromosome region 17p11.2p12 is frequently amplified in human high grade osteosarcomas (25% of cases), suggesting the presence ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000451046
更新日期:2016-01-01 00:00:00
abstract::Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc11...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000443625
更新日期:2015-01-01 00:00:00
abstract::Chromosomes in hymenopteran insects cannot currently be analysed in adult individuals. The only available cytogenetic techniques need to be performed in larvae. Here we develop and implement a SCAR (Sequence Characterized Amplified Region) marker, associated with B chromosomes in the bee Partamona helleri, which has p...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000097430
更新日期:2007-01-01 00:00:00
abstract::Chromosome homologies in reptiles have been investigated extensively by gene mapping and chromosome painting. Relative chromosome size can be estimated roughly from conventional karyotypes, but chromosome GC content cannot be evaluated by any of these approaches. However, GC content can be obtained by whole-genome seq...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000497265
更新日期:2019-01-01 00:00:00
abstract::X chromosome inactivation and DNA methylation are reviewed, with emphasis on the contributions of Susumu Ohno and the predictions made in my 1975 paper (Riggs, 1975), in which I proposed the "maintenance methylase" model for somatic inheritance of methylation patterns and suggested that DNA methylation would be involv...
journal_title:Cytogenetic and genome research
pub_type: 传,历史文章,杂志文章,评审
doi:10.1159/000071569
更新日期:2002-01-01 00:00:00
abstract::A 17-year-old girl presented with a distinct phenotype mainly featuring craniofacial dysmorphism, including a disproportioned large, round, elongated face; hypertelorism; deep-set eyes with short palpebral fissures; obesity (BMI 37), and a neuropsychiatric disorder with high-functioning autism. Postnatal conventional ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000481331
更新日期:2017-01-01 00:00:00
abstract::A single female with 206 chromosomes and another 26 females with 156 chromosomes identified as Prussian carp, Carassius gibelio, and 5 individuals with 100 chromosomes identified as crucian carp, C. carassius, were sampled during field survey in one locality in the upper Elbe River. To identify the origin of females w...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000350689
更新日期:2013-01-01 00:00:00
abstract::The occurrence of interchromosomal effects (ICE) in reciprocal translocation carriers still remains contradictory in the human literature. We used the pig as an animal model to investigate whether the structure of the reciprocal translocations as well as the size and/or type of the chromosomes not involved in the rear...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000245920
更新日期:2009-01-01 00:00:00
abstract::A comparative cytogenetic analysis was carried out on four Hylinae tree frogs from Brazil ((Aparasphenodon brunoi, Corythomantis greeningi, Osteocephalus langsdorffii, and Scinax fuscovarius) using Giemsa staining, BrdU replication banding, Ag-NOR staining, C-banding, DAPI and CMA(3) fluorochrome staining, and fluores...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076304
更新日期:2003-01-01 00:00:00
abstract::Sex-determining mechanisms (SDMs) set an individual's sexual fate by its genotype (genotypic sex determination, GSD) or environmental factors like temperature (temperature- dependent sex determination, TSD), as in turtles where the GSD "trigger" remains unknown. SDMs co-evolve with turtle chromosome number, perhaps be...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000497302
更新日期:2019-01-01 00:00:00
abstract::We have identified LIM-kinase (LIMK1 and LIMK2), the only known catalytic protein among LIM-family molecules. Both LIMK1 and LIMK2 phosphorylate (inactivate) cofilin, an actin depolymerizing factor, and induce actin cytoskeleton reorganization. We as well as others concurrently demonstrated that LIMK activation was re...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000076815
更新日期:2003-01-01 00:00:00
abstract::We describe 7 cases of abnormal karyotypes involving chromosomes Y and 15 in Ethiopian Beta Israel patients: 46,XX, der(15)t(Y;15)(q12;p12) and 46,XY,der(15)t(Y;15)(q12;p12). Six cases were incidentally found in amniocentesis performed for various indications; the indication for karyotyping in 1 case was recurrent abo...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000336201
更新日期:2012-01-01 00:00:00
abstract::Interspaced repetitive DNA elements and segmental duplications have been extensively analyzed in fishes through physical chromosome mapping methods, providing a better comprehension of the structure and organization of the genome of this group. In order to contribute to this scenario, a sequence integration study of d...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000353845
更新日期:2013-01-01 00:00:00
abstract::In an attempt to analyze the organization of repetitive DNAs in the amphibian genome, 7 microsatellite motifs and a 5S rDNA sequence were synthesized and mapped in the karyotypes of 5 Amolops species. The results revealed nonrandom distribution of the microsatellite repeats, usually in the heterochromatic regions, as ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000499416
更新日期:2019-01-01 00:00:00
abstract::Scanning electron microscopy (SEM) proves to be an appropriate technique for imaging chromatin organization in meiosis I and II of rye (Secale cereale) down to a resolution of a few nanometers. It could be shown for the first time that organization of basic structural elements (coiled and parallel fibers, chromomeres)...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078021
更新日期:2004-01-01 00:00:00
abstract::We analyzed genetic changes in condylomas (four cases), vulvar intraepithelial neoplasia I-III (VIN I-III, eleven cases), and primary vulvar squamous cell carcinomas (VSCC, ten cases) by high-resolution comparative genomic hybridization (HR-CGH) and flowcytometry. All samples were also human papilloma virus (HPV)-geno...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000078559
更新日期:2004-01-01 00:00:00
abstract::The Hodgkin cell line U-HO1 was established from a malignant pleural effusion of a 23-year-old male patient during the end stage of refractory nodular sclerosing classical Hodgkin lymphoma (cHL). Since its establishment in 2005, U-HO1 has maintained stable characteristics in vitro and has a doubling time of about 4 da...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000112062
更新日期:2007-01-01 00:00:00
abstract::Sperm chromosome abnormalities cut across a number of areas relevant to ICC XV. The association between increased levels of sperm aneuploidy (usually disomy) and male infertility has implications for the sessions on reproduction, sex chromosomes, aneuploidy and meiosis and was, to the best of our knowledge, first repo...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000079575
更新日期:2004-01-01 00:00:00
abstract::Huntington's disease (HD) is a genetically dominant neurodegenerative condition caused by an unique mutation in the disease gene huntingtin. Although the Huntington protein (Htt) is ubiquitously expressed, expansion of the polyglutamine tract in Htt leads to the progressive loss of specific neuronal subpopulations in ...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章,评审
doi:10.1159/000072864
更新日期:2003-01-01 00:00:00
abstract::Gross chromosomal rearrangements and aneuploidy are among the most common somatic genomic abnormalities that occur during cancer initiation and progression, in particular in human solid tumor carcinogenesis. The loss of large chromosomal regions as consequence of gross rearrangements (e.g. deletions, monosomies, unbal...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000072844
更新日期:2003-01-01 00:00:00
abstract::Phyllotines are sigmodontine rodents endemic to South America with broad genetic variability, Robertsonian polymorphisms being the most frequent. Moreover, this taxon includes a species with multiple sex chromosomes, which is infrequent in mammals. However, molecular cytogenetic techniques have never been applied to p...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000444602
更新日期:2015-01-01 00:00:00
abstract::Peroxisomes are cell organelles that perform multiple functions in the metabolism of lipids and of reactive oxygen species. They are present in most eukaryotic cells. However, they are believed to be absent in spermatozoa and they have never been described in male germ cells. We have used the immortalized germ cell li...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000076825
更新日期:2003-01-01 00:00:00
abstract::A 41-year-old Asian woman with bilateral renal angiomyolipomas (AML) was incidentally identified to have a balanced translocation, 46,XX,t(11;12)(p15.4;q15). She had no other features or family history to suggest a diagnosis of tuberous sclerosis. Her healthy daughter had the same translocation and no renal AML at the...
journal_title:Cytogenetic and genome research
pub_type: 杂志文章
doi:10.1159/000479463
更新日期:2017-01-01 00:00:00