Abstract：
:Proteins are the primary effectors of cellular function, including cellular metabolism, structural dynamics, and information processing. However, quantitative characterization of proteins at the single-cell level is challenging due to the tiny amount of protein available. Here, we present Abseq, a method to detect and quantitate proteins in single cells at ultrahigh throughput. Like flow and mass cytometry, Abseq uses specific antibodies to detect epitopes of interest; however, unlike these methods, antibodies are labeled with sequence tags that can be read out with microfluidic barcoding and DNA sequencing. We demonstrate this novel approach by characterizing surface proteins of different cell types at the single-cell level and distinguishing between the cells by their protein expression profiles. DNA-tagged antibodies provide multiple advantages for profiling proteins in single cells, including the ability to amplify low-abundance tags to make them detectable with sequencing, to use molecular indices for quantitative results, and essentially limitless multiplexing.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Shahi P,Kim SC,Haliburton JR,Gartner ZJ,Abate ARdoi
10.1038/srep44447subject
Has Abstractpub_date
2017-03-14 00:00:00pages
44447issn
2045-2322pii
srep44447journal_volume
7pub_type
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更新日期：2016-06-30 00:00:00
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pub_type： 杂志文章
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更新日期：2014-01-14 00:00:00
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更新日期：2017-03-15 00:00:00
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pub_type： 杂志文章,meta分析,评审
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pub_type： 已发布勘误
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pub_type： 杂志文章
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