Abstract:
OBJECTIVES:Eosinophilic granulomatosis with polyangiitis (EGPA) is associated with an inflammation and the presence of antineutrophil cytoplasmic antibodies (ANCA). Thus, we investigated the impact of ANCAs and eosinophilic inflammation on neutrophil activation and extracellular traps (NETs) formation. METHODS:We recruited 29 patients in the remission of EGPA (17 ANCA-negative and 12 ANCA-positive, including 7 p-ANCA-positive and 5 c-ANCA-positive patients). Healthy donors' neutrophils were stimulated with EGPA patients' serum. NETs formation was assessed by immunofluorescence and scanning electron microscopy. RESULTS:EGPA patients presented enhanced ability to generate NETs compared to healthy subjects (20.3±8.2% vs. 2.7±1.5%, p=0.0036). However, there were no differences in NETs formation between ANCA-positive and ANCA-negative patients (23±11.2% vs. 17±6.1%, p=0.15). There was also no correlation between NETs generation and the amount of circulating DNA in EGPA patients. Among ANCA-positive patients, p-ANCA-positives showed the highest percentage of NETs as compared to cANCA-positive and ANCA-negative patients (27.3±10.3% vs. 17.8±10.5% and vs. 17±6.1%, both p<0.01, respectively). Eosinophils number correlated with the percentage of NETs in the whole EGPA group (r=0.53, p=0.039), but we failed to observe the correlation with an eosinophil cationic protein (r=0.49, p=0.058). CONCLUSIONS:EGPA patients' serum has the ability to induce NETosis with no regard to the ANCA status in contrast to other vasculitides, where p-ANCA were considered as the main factor. Interestingly, NETs formation in EGPA patients connected with the number of eosinophils might be of major relevance. Further studies are required to assess which eosinophil-derived factors might be responsible for the neutrophils activation in EGPA patients.
journal_name
Clin Exp Rheumatoljournal_title
Clinical and experimental rheumatologyauthors
Natorska J,Ząbczyk M,Siudut J,Krawiec P,Mastalerz L,Undas Asubject
Has Abstractpub_date
2017-03-01 00:00:00pages
27-32issue
1eissn
0392-856Xissn
1593-098Xpii
10651journal_volume
35 Suppl 103pub_type
杂志文章abstract::The threshold for pharmacological intervention for osteoporosis remains controversial. Tools predicting the future risk of new fractures are increasingly used to establish a convenient individual risk/benefit ratio for a long term treatment. FRAX® is likely to become the most widely used tool for assessing fracture ri...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
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abstract:OBJECTIVES:To provide a detailed description of the ultrasound (US) scanning protocol to measure the muscle thickness in the forearm and to test its feasibility and interobserver reliability. METHODS:Four rheumatologists trained in musculoskeletal US carried out the examinations in 45 subjects (30 consecutively enroll...
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journal_title:Clinical and experimental rheumatology
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doi:
更新日期:1998-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2004-11-01 00:00:00
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journal_title:Clinical and experimental rheumatology
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
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doi:
更新日期:2020-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1997-05-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
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journal_title:Clinical and experimental rheumatology
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doi:
更新日期:2000-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2014-05-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2012-03-01 00:00:00
abstract::A similar disease severity among men and women in Brasil, a high frequency of gastrointestinal involvement in China, Japan and USA, a low frequency of pathergy positivity in Japan and USA underline the ethnic variations reported in recent studies. Polymorphisms pertaining both to innate and adaptive immunity in genome...
journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,评审
doi:
更新日期:2012-05-01 00:00:00
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doi:
更新日期:2020-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
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doi:
更新日期:2018-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
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doi:
更新日期:2013-07-01 00:00:00
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journal_title:Clinical and experimental rheumatology
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doi:
更新日期:2009-03-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,多中心研究
doi:
更新日期:2020-09-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
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journal_title:Clinical and experimental rheumatology
pub_type: 杂志文章,多中心研究
doi:
更新日期:2014-07-01 00:00:00