Abstract:
BACKGROUND:Platelet-rich fibrin (PRF) has been widely used in oral implantology and other fields, but benefits of the fresh PRF (FPRF (fresh platelet-rich fibrin)) were consequently limited because of its short-term application. Thus, a protocol for the combination of PRF and lyophilization comes up in the present study to address the issue of PRF storage and delayed clinical application, which has little been reported in this field at home and abroad by now. PURPOSE:The aim of the present study was to evaluate the applicability of lyophilized platelet-rich fibrin (Ly-PRF) used as the scaffold material for craniofacial tissue regeneration and to compare its biochemical properties with commonly used fresh PRF. MATERIALS AND METHODS:Two volunteers with both genders were selected as the source of PRF and Ly-PRF samples. Macro- and micro-scopic appearance evaluation as well as immunohistochemical comparison were performed on PRF samples before and after freeze-drying at -196°C. The second experimental phase was to observe clinical performance when fresh and lyophilized PRF were applied in guided bone regeneration (GBR) operations in 39 patients losing teeth in the anterior maxillary region who required an oral implantation followed by labial bone grafting. RESULTS:The conventional histological and transmission electron microscopy images showed the microstructure of Ly-PRF, which resembled a mesh containing apparently irregularly shaped platelets with less alpha-granule than fresh PRF in micro and a translucent membrane with less elasticity than fresh PRF in macro. Simultaneous immunohistological staining results showed positive expression of PDGF-BB, IL-1, IL-4, TNF, TGF-β1 in both fresh and lyophilized PRF, while the expression of PDGF-BB, IL-1, TNF, TGF-β1 has no statistical difference between them (P > .05) but that of IL-4 in Ly-PRF is statistically higher than in fresh PRF (P < .05). When applied in GBR operations, there were no significant differences between Ly-PRF and FPRF in factors of histological and clinical evaluations (i.e., color, swelling, bleeding of the mucosa, pain leveland, and remodeling of hard tissue) performed 3 days, 7 days, and 4 months after the surgery (P > .05). CONCLUSIONS:This study strongly supports that lyophilization at -196°C does not largely influence the expression of bioactive factors, the microstructure of fibrinogen or the clinical effects of PRF.
journal_name
Clin Implant Dent Relat Resjournal_title
Clinical implant dentistry and related researchauthors
Zhang J,Qi X,Luo X,Li D,Wang H,Li Tdoi
10.1111/cid.12473subject
Has Abstractpub_date
2017-06-01 00:00:00pages
466-477issue
3eissn
1523-0899issn
1708-8208journal_volume
19pub_type
杂志文章abstract:PURPOSE:To develop numerical and experimental methods for investigating the formation of micro-gaps and the change in contact area at the implant-abutment interface of two different connector designs under oblique cyclic loading. MATERIALS AND METHODS:Samples (n = 10 per group) of two-piece implant systems with the co...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12792
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:Although increase of misfit has been reported when associating implant and abutment from different manufacturers, Procera custom abutment has been universally used in clinical practice. PURPOSE:The purpose of this investigation was to compare the vertical gap of zirconia Procera® abutment associated with im...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/j.1708-8208.2010.00323.x
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:The technical development of digital processing allows the production of anatomically full-contoured implant-supported restorations. PURPOSE:The aim of this prospective clinical trial was to analyze the treatment concept of monolithic lithium disilicate (LS2) single-unit restorations in a complete digital w...
journal_title:Clinical implant dentistry and related research
pub_type: 临床试验,杂志文章
doi:10.1111/cid.12472
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Immediate/early loading protocols are becoming frequently used in implant dentistry, but the prerequisites for achieving good results and the limitations of such protocols are not fully known. Moreover, the terminology used in immediate/early loading is still confusing. PURPOSE:The purpose of this article i...
journal_title:Clinical implant dentistry and related research
pub_type: 共识发展会议,杂志文章,评审
doi:10.1111/j.1708-8208.2003.tb00183.x
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Intrasocket reactive soft tissue can be used for primary closure during augmentation of infected extraction sites exhibiting severe bone loss prior to implant placement. The present study evaluated the histological characteristics of the initially used intrasocket reactive soft tissue, the overlying soft tis...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/j.1708-8208.2010.00281.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Although many studies have been published on single implants, long-term data remain scarce. PURPOSE:To evaluate immediately restored single implants after at least 8 years of follow-up in terms of buccal bone, soft tissue alterations, aesthetic ratings, and patient-reported outcomes. MATERIALS AND METHODS:...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12613
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Conventional wisdom states that reactive soft tissue consisting of granulation tissue and long junctional epithelium in compromised sites should be curetted completely, while others approve of its preservation in implant surgeries. PURPOSE:To evaluate the presence of reactive soft tissue as wound closure fo...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12845
更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:It is postulated that peri-implant sulcular fluid (PISF) levels of advanced glycation end products (AGEs) are higher with high glycemic levels. PURPOSE:In the present clinico-biochemical study, we explored the clinical and radiographic peri-implant parameters and levels of AGEs among prediabetic, type 2 dia...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12607
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:The aim of this preclinical study was to analyze and compare different grafting techniques with respect to volume stability after wound closure. MATERIALS AND METHODS:Four different grafting techniques were evaluated in vitro for volume stability in a one-wall horizontal defect configuration. Group 1: guide...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12728
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Bone allografts have shown satisfactory clinical results in alveolar ridge reconstructions. However, the process of incorporation and the resorption rates of these grafts are not yet fully understood. PURPOSE:The aim of this study was to use computed tomography (CT), histology, and histomorphometry to asses...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章,随机对照试验
doi:10.1111/cid.12108
更新日期:2015-04-01 00:00:00
abstract:PURPOSE:This prospective study was performed to evaluate the outcomes of XiVE® S plus implants (Dentsply Friadent, Mannheim, Germany) following conventional restoration with bar structures and overdentures in the edentulous mandible. MATERIALS AND METHODS:A total of 39 patients were treated with four interforaminal im...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/j.1708-8208.2011.00380.x
更新日期:2013-08-01 00:00:00
abstract:UNLABELLED:Osteonecrosis of the jaw (ONJ) is a severe complication of bisphosphonate treatment. PURPOSE:A detailed characterization of sampled peri-necrotic jawbone from bisphosphonate-treated patients was performed at tissue and cellular level (histological analyses and gene expression). MATERIALS AND METHODS:Alveol...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12307
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Bone morphogenetic protein-2 (BMP2) has been successfully utilized in dentistry to promote new bone formation because of its osteoinductive ability to recruit mesenchymal progenitor cells and induce their differentiation to bone-forming osteoblasts. Recently, novel biphasic calcium phosphate scaffolds have b...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12384
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Data on risk factors and complications after long-term implant treatment is limited. The aims were to evaluate the role of various fixation modes and to analyze complications and risks that affect long-term use of implant-supported partial fixed dental prostheses. MATERIALS AND METHODS:Fifty partially edent...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12587
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Reactive oxygen species contribute to periodontal tissue homeostasis under control of anti-oxidative responses. Disruption in this balance induces severe inflammation and extended tissue degradation. PURPOSE:Aim of this study was to identify the expression levels of nuclear factor, erythroid 2 like 2 (NFE2L...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12619
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Elicitation of the relationship of periodontopathogens and pro-inflammatory cytokines to bone resorption and formation is significant to a growing body of research known as osteoimmunology. It is essential that clinically healthy peri-implant and periodontal sites are studied to contribute comparison data fo...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/j.1708-8208.2007.00076.x
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND:There have been few studies investigating the long-term outcome of early-loaded implants with segmented bridgeworks on fully edentulous maxillae. PURPOSE:To evaluate the long-term predictability of early-loaded Straumann implant-supported fixed segmented bridgeworks in edentulous maxillae. MATERIAL AND MET...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12420
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Peri-implant mucositis is very common and, when left untreated, can progress to the more serious condition of peri-implantitis. Therefore, early diagnosis, adequate treatment and, in particular, adherence to a peri-implant supportive therapy are extremely important for the management of peri-implant mucositi...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12833
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Recently, the present team reported the 1-year data of one-stage surgery and mainly early loading performed in edentulous mandibles using 750 turned Brånemark System implants in 152 patients. PURPOSE:The aim of the present investigation was to retrospectively evaluate the 1-year results of the same treatmen...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/j.1708-8208.2007.00060.x
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:It is hypothesized that peri-implant conditions are worse with increasing severity of obesity, because systemic low-grade inflammatory marker (C-reactive protein [CRP]) is higher in severe form of obese individuals. PURPOSE:The aim of the cross-sectional retrospective study was to compare clinical and radio...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12556
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:There is a need for more studies investigating the outcome of implant-supported rehabilitations in patients with systemic disorders. MATERIALS AND METHODS:This retrospective clinical study included 721 patients with systemic disorders or smoking habits (422 females; 299 males), with an average age of 51 yea...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12346
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:The study aims to determine if midline mandibular dental implants pose a risk for the midline lingual canal (MLC). MATERIALS AND METHODS:Cone beam computed tomography was used to scan 122 mandibles (31 black males; 28 black females; 32 white males and 31 white females). Midsagittal sections in the reconstructe...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12080
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:It is postulated that clinical and radiographic peri-implant parameters are worse and levels of interleukin (IL)-1β and matrix metalloproteinase (MMP)-9 in the peri-implant sulcular fluid (PISF) are higher in cigarette-smokers (CS) and smokeless-tobacco users (STU) compared with nontobacco user (NTU). PURPO...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12575
更新日期:2018-02-01 00:00:00
abstract:PURPOSE:To prospectively assess the outcome of immediate rehabilitation of extremely atrophic mandibles by a full-arch fixed bridge anchored to four implants. MATERIAL AND METHODS:Twenty patients with edentulous mandibles were included in the study. Each patient received a full-arch fixed bridge supported by two axial...
journal_title:Clinical implant dentistry and related research
pub_type: 临床试验,杂志文章
doi:10.1111/j.1708-8208.2009.00265.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:In the Literature, there are several studies demonstrating that infraposition happens also in adult patients. PURPOSE:To conduct a retrospective evaluation of infraocclusion of implant-retained crowns in the anterior maxilla of adult patients and of the patient awareness and perception of the problem. MATE...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12790
更新日期:2019-08-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate a possible biomarker for bisphosphonate-related osteonecrosis of the jaw (BRONJ) in an animal model. MATERIALS AND METHODS:Forty-eight Sprague-Dawley rats were randomly divided into the bisphosphonate group (n = 36), who were injected once a week with zoledronic acid, a...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12297
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:A real-time surgical navigation system potentially increases the accuracy when used for quad-zygomatic implant placement. PURPOSE:To evaluate the accuracy of a real-time surgical navigation system when used for quad zygomatic implant placement. MATERIALS AND METHODS:Patients with severely atrophic maxillae...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12475
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Bovine-derived grafting materials are frequently used in a variety of bone augmentation techniques. The aim of this paper is to assess the unique safety issue of bovine-derived grafting materials that is rarely addressed in dental literature: risk of bovine spongiform encephalopathy (BSE). METHODS:The valid...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12391
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Association between long-term structural changes of grafted bone and outcomes of the implants is not clear. PURPOSE:Morphometrical measurements and implant success were analyzed in subjects of particulate cellular bone and marrow (PCBM) reconstruction of the jawbone. MATERIALS AND METHODS:Subjects were 30 ...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/cid.12723
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:By using the guided tissue regeneration concept it is possible to augment bone, beyond the skeletal envelope, provided certain biologic, surgical, and barrier-related demands are met. Among barrier-related factors of importance are the surface properties. PURPOSE:The aim of this study was to evaluate whethe...
journal_title:Clinical implant dentistry and related research
pub_type: 杂志文章
doi:10.1111/j.1708-8208.1999.tb00090.x
更新日期:1999-01-01 00:00:00