Prolonged maternal separation attenuates BDNF-ERK signaling correlated with spine formation in the hippocampus during early brain development.

abstract：:Cell and tissue concentrations of NO2- and NO3- are important indicators of nitric oxide synthase activity and crucial in the regulation of many metabolic functions, as well as in nonenzymatic nitric oxide release. We adapted the capillary electrophoresis technique to quantify NO2- and NO3- levels in single identified...

journal_title：Journal of neurochemistry

authors： Cruz L,Moroz LL,Gillette R,Sweedler JV

更新日期：1997-07-01 00:00:00

abstract：:Glycine and GABA are likely co-transmitters in the spinal cord. Their possible interactions in presynaptic terminals have, however, not been investigated. We studied the effects of glycine on GABA release using superfused mouse spinal cord synaptosomes. Glycine concentration dependently elicited [(3)H]GABA release whi...

journal_title：Journal of neurochemistry

authors： Raiteri L,Raiteri M,Bonanno G

更新日期：2001-03-01 00:00:00

abstract：:Progression of α-synuclein inclusion pathology may occur through cycles of release and uptake of α-synuclein aggregates, which induce additional intracellular α-synuclein inclusion pathology. This process may explain (i) the presence of α-synuclein inclusion pathology in grafted cells in human brains, and (ii) the slo...

journal_title：Journal of neurochemistry

authors： Sacino AN,Brooks MM,Chakrabarty P,Saha K,Khoshbouei H,Golde TE,Giasson BI

更新日期：2017-02-01 00:00:00

abstract：:The effect of hypoglycaemic, hypoxic, and ischaemic conditions on high-affinity neurotransmitter transport was studied in the human astrocytoma clone D384 and the human neuroblastoma clone SH-SY5Y. Both cell lines expressed a sodium-dependent glutamate/aspartate transporter. Km values for D-[3H]aspartate uptake were 6...

journal_title：Journal of neurochemistry

authors： O'Neill CM,Ball SG,Vaughan PF

更新日期：1994-08-01 00:00:00

abstract：:The 17-kDa endogenous brain protein glia maturation factor (GMF) was transfected into C6 rat glioma cells using a replication-defective human adenovirus vector. The cells overexpressed GMF but did not secrete the protein into the medium. Transfection with GMF led to the activation of the transcription factor nuclear f...

journal_title：Journal of neurochemistry

authors： Lim R,Zaheer A,Yorek MA,Darby CJ,Oberley LW

更新日期：2000-02-01 00:00:00

abstract：:Here, we examined whether amyloid-beta (Abeta) protein participates in cell death and retinal function using three types of transgenic (Tg) mice in vivo [human mutant amyloid precursor protein (APP) Tg (Tg 2576) mice, mutant presenilin-1 (PS-1) knock-in mice, and APP/PS-1 double Tg mice]. ELISA revealed that the insol...

journal_title：Journal of neurochemistry

authors： Shimazawa M,Inokuchi Y,Okuno T,Nakajima Y,Sakaguchi G,Kato A,Oku H,Sugiyama T,Kudo T,Ikeda T,Takeda M,Hara H

更新日期：2008-10-01 00:00:00

abstract：:N-Methyl-D-aspartate (NMDA) receptors mediate increases in intracellular calcium that can be modulated by protein kinase C (PKC). As PKC modulation of NMDA receptors in neurons is complex, we studied the effects of PKC activation on recombinant NMDA receptor-mediated calcium rises in a nonneuronal mammalian cell line,...

journal_title：Journal of neurochemistry

authors： Grant ER,Bacskai BJ,Anegawa NJ,Pleasure DE,Lynch DR

更新日期：1998-10-01 00:00:00

abstract：:We have identified and cataloged 54 genes that exhibit predominant expression in the striatum. Our hypothesis is that such mRNA molecules are likely to encode proteins that are preferentially associated with particular physiological processes intrinsic to striatal neurons, and therefore might contribute to the regiona...

journal_title：Journal of neurochemistry

authors： Desplats PA,Kass KE,Gilmartin T,Stanwood GD,Woodward EL,Head SR,Sutcliffe JG,Thomas EA

更新日期：2006-02-01 00:00:00

abstract：:We report the isolation of cDNA clones encoding the somatic form of the E1 alpha subunit of the pyruvate dehydrogenase complex of rat. The deduced amino acid sequence has 99.5, 98, and 97% identity, respectively, with the orthologous proteins of mouse, human, and pig and 98.5% identity with a rat E1 alpha sequence rep...

journal_title：Journal of neurochemistry

authors： Cullingford TE,Clark JB,Phillips IR

更新日期：1994-05-01 00:00:00

abstract：:Huntington's disease and six other neurodegenerative diseases are associated with abnormal gene products containing expanded polyglutamine (poly-Q; Qn) domains (n > or = 40). In the present work, we show that glutathione S-transferase (GST) fusion proteins containing a small, physiological-length poly-Q domain (GSTQ10...

journal_title：Journal of neurochemistry

authors： Cooper AJ,Sheu KF,Burke JR,Onodera O,Strittmatter WJ,Roses AD,Blass JP

更新日期：1997-07-01 00:00:00

abstract：:Previous studies have purified from brain a Ca2+/calmodulin-dependent protein kinase II (designated CaM-kinase II) that phosphorylates synapsin I, a synaptic vesicle-associated phosphoprotein. CaM-kinase II is composed of a major Mr 50K polypeptide and a minor Mr 60K polypeptide; both bind calmodulin and are phosphory...

journal_title：Journal of neurochemistry

authors： Shields SM,Vernon PJ,Kelly PT

更新日期：1984-12-01 00:00:00

abstract：:Fragile X syndrome (FXS) is a developmental disorder caused by the loss of Fragile X Mental Retardation 1 (FMR1) gene function because of a CGG repeat expansion (> 200 repeats) in the gene. The molecular mechanism(s) linking loss of FMR1 function to the molecular pathology and cognitive/behavioral disability remain un...

journal_title：Journal of neurochemistry

authors： Wang X,Snape M,Klann E,Stone JG,Singh A,Petersen RB,Castellani RJ,Casadesus G,Smith MA,Zhu X

更新日期：2012-05-01 00:00:00

abstract：:We have previously shown that prolonged exposure to neurotrophins induces oxidative neuronal death. In the present study, we further examined the cascades involved in neurotrophin-4/5 (NT-4/5)-induced neuronal death. Exposure of mature cortical cultures for 48 h to NT-4/5 induced neuronal death through TrkB activation...

journal_title：Journal of neurochemistry

authors： Choi SY,Hwang JJ,Koh JY

更新日期：2004-02-01 00:00:00

abstract：:We identified, by affinity chromatography, two putative binding proteins for the presynaptic snake venom toxin taipoxin. We have previously characterized one of these proteins [neuronal pentraxin (NP)] as a neuronally secreted protein with homology to acute-phase proteins. Here we report the identification of the seco...

journal_title：Journal of neurochemistry

authors： Dodds D,Schlimgen AK,Lu SY,Perin MS

更新日期：1995-05-01 00:00:00

abstract：:In a study employing mouse brain synaptosomes and synaptosomal sonicates, the complete metabolic machinery was found to be present for transport of arginine into synaptosomes, its conversion to ornithine, and the formation from the latter of glutamic acid, gamma-aminobutyric acid, and proline. The results show that a ...

journal_title：Journal of neurochemistry

authors： Johnson JL,Roberts E

更新日期：1984-04-01 00:00:00

abstract：:The deficits in Alzheimer disease (AD) stem at least partly from neurotoxic beta-amyloid peptides generated from the amyloid precursor protein (APP). APP may also be cleaved intracellularly at Asp664 to yield a second neurotoxic peptide, C31. Previously, we showed that cleavage of APP at the C-terminus is required for...

journal_title：Journal of neurochemistry

authors： Nguyen TV,Galvan V,Huang W,Banwait S,Tang H,Zhang J,Bredesen DE

更新日期：2008-02-01 00:00:00

abstract：:The effect of inhibition and down-regulation of protein kinase C (PKC) subtypes alpha, epsilon, and zeta on noradrenaline (NA) secretion from human SH-SY5Y neuroblastoma cells was investigated. The PKC inhibitor Ro 31-7549 inhibited carbachol-evoked NA release (IC(50) 0.6 microM) but not 100 mM (K+)-evoked release. In...

journal_title：Journal of neurochemistry

authors： Turner NA,Walker JH,Ball SG,Vaughan PF

更新日期：1996-06-01 00:00:00

abstract：:Chronic alcohol exposure induces lasting behavioral changes, tolerance, and dependence. This results, at least partially, from neural adaptations at a cellular level. Previous genome-wide gene expression studies using pooled human brain samples showed that alcohol abuse causes widespread changes in the pattern of gene...

journal_title：Journal of neurochemistry

authors： Liu J,Lewohl JM,Dodd PR,Randall PK,Harris RA,Mayfield RD

更新日期：2004-09-01 00:00:00

abstract：:Induction of the prodynorphin gene occurs in a tissue-specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP-responsive element 2 (DynCRE2), Dyn...

journal_title：Journal of neurochemistry

authors： Carrión AM,Mellström B,Luckman SM,Naranjo JR

更新日期：1998-03-01 00:00:00

abstract：:Exposure to excessive levels of light induces photoreceptor apoptosis and can be a causative factor in age-related macular degeneration (AMD). However, the cellular events that mediate this apoptotic response are poorly understood. Here, we investigated the roles of endoplasmic reticulum (ER) stress in light-induced c...

journal_title：Journal of neurochemistry

authors： Nakanishi T,Shimazawa M,Sugitani S,Kudo T,Imai S,Inokuchi Y,Tsuruma K,Hara H

更新日期：2013-04-01 00:00:00

abstract：:The distribution of basal and of H2O2-stimulated cyclooxygenase activity in the primary fractions of rat brain homogenates and in the subfractions of crude mitochondrial fraction was studied. For comparison, the localization of H2O2-generating monoamine oxidase (MAO) as well as that of the mitochondrial marker succina...

journal_title：Journal of neurochemistry

authors： Seregi A,Serfözö P,Mergl Z

更新日期：1983-02-01 00:00:00

abstract：:We investigated the regulatory pathways responsible for agonist-induced internalization and down-regulation of G(q) protein-coupled histamine H(1)-receptors in Chinese hamster ovary cells. Histamine-induced internalization and down-regulation of H(1)-receptors were detected as the loss of [(3)H]mepyramine binding site...

journal_title：Journal of neurochemistry

authors： Hishinuma S,Komazaki H,Fukui H,Shoji M

更新日期：2010-05-01 00:00:00

abstract：:Epilepsy is a serious neurological condition exhibiting complex pathology and deserving of more serious attention. More than 30% of people with epilepsy are not responsive to more than 20 anti-epileptic drugs currently available, reflecting an unmet clinical need for novel therapeutic strategies. Not much is known abo...

journal_title：Journal of neurochemistry

authors： Paudel YN,Semple BD,Jones NC,Othman I,Shaikh MF

更新日期：2019-12-01 00:00:00

abstract：:The mechanisms by which chronic nicotine self-administration augments hypothalamo-pituitary-adrenal (HPA) responses to stress are only partially understood. Nicotine self-administration alters neuropeptide expression in corticotropin-releasing factor (CRF) neurons within paraventricular nucleus (PVN) and increases PVN...

journal_title：Journal of neurochemistry

authors： Yu G,Chen H,Wu X,Matta SG,Sharp BM

更新日期：2010-05-01 00:00:00

abstract：:Cell culture studies have shown that the Alzheimer amyloid precursor protein (APP) is secreted after full-length APP is cleaved by a putative secretase at the Lys16-Leu17 bond (secretase cleavage I) of the amyloid peptide sequence. Because this cleavage event is incompatible with amyloid production, it has been assume...

journal_title：Journal of neurochemistry

authors： Anderson JP,Chen Y,Kim KS,Robakis NK

更新日期：1992-12-01 00:00:00

abstract：:Acetyltransferase enzymatic activity was detected and measured in homogenates obtained from intact nerve fibers and their saparate cellular components, in the tropical squid Sepioteuthis sepioidea. The levels of acetylcholine synthesis were determined in pooled samples of whole stellar nerve, intact giant nerve fiber,...

journal_title：Journal of neurochemistry

authors： Heumann R,Villegas J,Herzfeld DW

更新日期：1981-02-01 00:00:00

abstract：:Mitochondrial respiratory function, assessed from the rate of oxygen uptake by homogenates of rat brain subregions, was examined after 30 min of forebrain ischemia and at recirculation periods of up to 48 h. Ischemia-sensitive regions which develop extensive neuronal loss during the recirculation period (dorsal-latera...

journal_title：Journal of neurochemistry

authors： Sims NR,Pulsinelli WA

更新日期：1987-11-01 00:00:00

abstract：:The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis. In this study, we address regulation of CHT function by plasma membrane cholesterol. We...

journal_title：Journal of neurochemistry

authors： Cuddy LK,Winick-Ng W,Rylett RJ

更新日期：2014-03-01 00:00:00

abstract：UNLABELLED:J. Neurochem. (2012) 121, 597-606. ABSTRACT:In cultured rat neocortical interneurons, we have studied the effect of long-term application of NMDA or AMPA on the surface density of the NMDA (GluN) receptor subunits GluN1 and GluN2B. Stimulation of Ca(2+) -permeable AMPA (GluA) receptors located on the intern...

journal_title：Journal of neurochemistry

authors： Meyer DK,Lindemeyer AK,Wilmes T,Sobottka H,Nörenberg W

更新日期：2012-05-01 00:00:00

abstract：:This study examined changes in composition and concanavalin A (Con A) binding of axonally transported glycoproteins and their pronase-generated glycopeptides in regenerating garfish olfactory nerve. A previous study had demonstrated a regeneration-related increase in the proportion of [3H]glucosamine label in lower-mo...

journal_title：Journal of neurochemistry

authors： Cole GJ,Elam JS

更新日期：1983-09-01 00:00:00