Abstract：
:Cell and tissue concentrations of NO2- and NO3- are important indicators of nitric oxide synthase activity and crucial in the regulation of many metabolic functions, as well as in nonenzymatic nitric oxide release. We adapted the capillary electrophoresis technique to quantify NO2- and NO3- levels in single identified buccal neurons and ganglia in the opisthobranch mollusc Pleurobranchaea californica, a model system for the study of the chemistry of neuron function. Neurons were injected into a 75-microm separation capillary and the NO2- and NO3- were separated electrophoretically from other anions and detected by direct ultraviolet absorbance. The limits of detection for NO2- and NO3- were <200 fmol (<4 microM in the neurons under study). The NO2- and NO3- levels in individual neurons varied from 2 mM (NO2-) and 12 mM (NO3-) in neurons histochemically positive for NADPH-diaphorase activity down to undetectable levels in many NADPH-diaphorase-negative cells. These results affirm the correspondence of histochemical NADPH-diaphorase activity and nitric oxide synthase in molluscan neurons. NO2- was not detected in whole ganglion homogenates or in hemolymph, whereas hemolymph NO3- averaged 1.8 +/- 0.2 x 10(-3) M. Hemolymph NO3- in Pleurobranchaea was appreciably higher than values measured for the freshwater pulmonate Lymnaea stagnalis (3.2 +/- 0.2 x 10(-5) M) and for another opisthobranch, Aplysia californica (3.6 +/- 0.7 x 10(-4) M). Capillary electrophoresis methods provide utility and convenience for monitoring NO2-/NO3- levels in single cells and small amounts of tissue.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Cruz L,Moroz LL,Gillette R,Sweedler JVdoi
10.1046/j.1471-4159.1997.69010110.xsubject
Has Abstractpub_date
1997-07-01 00:00:00pages
110-5issue
1eissn
0022-3042issn
1471-4159journal_volume
69pub_type
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1991-10-01 00:00:00
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更新日期：2018-12-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：1990-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-03-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1981.tb00491.x
更新日期：1981-08-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1996.67062292.x
更新日期：1996-12-01 00:00:00
abstract：:Rat retina tissue contains relatively high amounts of GD3 in relation to ganglio-series gangliosides even in the adult stages. This was attributed in part to an activity ratio between the enzyme that converts GM3 to GD3 [sialytransferase II (ST-II)] and the enzyme that converts GM3 to GM2 [N-acetylgalactosaminyltransf...
journal_title：Journal of neurochemistry
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更新日期：2001-04-01 00:00:00
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更新日期：2020-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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abstract：:Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1991-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2000-08-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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