Abstract:
:Valsartan (VAL), an antagonist of angiotensin II receptor type 1, has antihypertensive and multiple cardiovascular protective effects. The pleiotropic functions of VAL are related to the increased synthesis and biological activity of intravascular nitric oxide (NO). In this study, the role and mechanisms of VAL in the synthesis of NO were examined in human umbilical vein endothelial cells (HUVECs). Ten µmol/L of VAL was used to treat EA.hy926 cells for 30 minutes, 1, 3, 6, 12, and 24 hours, and three concentrations of VAL (i.e., 10, 1, and 0.1 µmol/L) were used to treat EA.hy926 cells for 24 hours. The cells were divided into five groups: control, VAL, VAL + Compound C (adenosine monophosphate-activated protein kinase [AMPK] inhibitor, 1 µmol/L), VAL + LY294002 (Akt [protein kinase B] inhibitor, 10 µmol/L), and VAL + L-nitro-arginine methyl ester (L-NAME, endothelial NO synthase [eNOS] inhibitor, 500 µmol/L) groups. The NO content in the VAL-treated HUVEC line (EA.hy926) was detected using the nitrate reductase method, and western blot was used to detect the phosphorylation of Akt, AMPK, and eNOS, as well as the changes in total protein levels. VAL increased NO synthesis in EA.hy926 cells in time- and dose-dependent manners (p < 0.05) and the intracellular phosphorylation levels of Akt, AMPK, and eNOS at the corresponding time points. LY294002, Compound C, and L-NAME could inhibit the VAL-promoted NO synthesis. VAL activated Akt, AMPK, and eNOS, thus promoting NO synthesis and playing a protective role in endothelial cells. These results partially explained the mechanisms underlying the cardiovascular protective effects of VAL.
journal_name
Bosn J Basic Med Scijournal_title
Bosnian journal of basic medical sciencesauthors
Zhao Y,Wang L,He S,Wang X,Shi Wdoi
10.17305/bjbms.2017.1319subject
Has Abstractpub_date
2017-05-20 00:00:00pages
132-137issue
2eissn
1512-8601issn
1840-4812journal_volume
17pub_type
杂志文章abstract::Kidney transplantation (Ktx) is generally performed during end stage renal disease due to a loss of the kidneys' ability to filter wastes from the circulatory system. Acute graft-versus-host disease (GVHD) after Ktx is a life-threatening complication that progresses to organ failure, systemic complications, and death....
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2011.2594
更新日期:2011-05-01 00:00:00
abstract::Early posttraumatic epilepsies (EPTE) are epileptic attacks that appear in first seven days after brain injury, with incidence of 3-5%. Predictors for development of EPTE are: impressive skull fracture with rupture of dura, intracranial haemorrhage, neurogical deficit (brain contusion), and posttraumatic amnesia longe...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2005.3234
更新日期:2005-11-01 00:00:00
abstract::Primary skin melanoma and skin cancers have been more prevalent in the previous decades and therefore have become a very significant public health problem. Dermatologists of the Skin and Venereal Diseases Clinic of the University of Sarajevo Clinics Centre initiated the first public preventive action called "Days of F...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2861
更新日期:2009-02-01 00:00:00
abstract::One of the most frequent hereditary causes of thrombophilia is, without a doubt, resistance to Activated Protein C (APC-resistance), which is a consequence of point mutation in gene coding for coagulation Factor V (Factor V Leiden) in 90-95% of cases. The aim of this paper was to determine prevalence of APC-resistance...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2789
更新日期:2009-11-01 00:00:00
abstract::A decision for operative versus nonoperative management of thoracolumbar fractures should NEVER be based solely on one factor. Only after a thorough physical, neurological, and spinal examination, and an assessment of a patient's prior activity, social and educational background and patient's expectations, one should ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:10.17305/bjbms.2005.3279
更新日期:2005-05-01 00:00:00
abstract::Growth hormone is essential for body growth but it also modulates metabolic pathways as well as neural, reproductive, immune, cardiovascular, and pulmonary functions. Numerous beneficial effects of growth hormone have led to its expanded therapeutic use in both children and adults. There are several officially approve...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:10.17305/bjbms.2004.3365
更新日期:2004-10-01 00:00:00
abstract::The aim of this study is to compare two major urological procedures in terms of patient exposure to radiation. We evaluated 175 patients, that were subjected to retrograde ureteroscopy (URS) and extracorporeal shock waves lithotripsy (ESWL) for lumbar or pelvic ureteral lithiasis, at two urological departments. The C-...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2014.4.99
更新日期:2014-10-18 00:00:00
abstract:BACKGROUND AND PURPOSE:Clinical research of drugs is a researching step subsequent to the preclinical studies in experimental animals. The aim of our research was to evaluate animal model of wound healing process after the burn inducement and effects of the ointment containing natural plants on the process of burn heal...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2003.3495
更新日期:2003-11-01 00:00:00
abstract::Most acute promyelocytic leukemia (APL) are characterized by reciprocal translocations t(15;17)(q22;21), which results in the fusion of PML gene at 15q22 with RARα gene at 17q21. However, several complex variant translocations also have been reported. Here we report a 62-year-old man with typical morphology and clinic...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:10.17305/bjbms.2020.5106
更新日期:2020-10-09 00:00:00
abstract::The analgesic benefit of melatonin and vitamin C as primary or adjuvant agents has been reported in various studies; however, their analgesic effects in the treatment of postoperative pain remain unclear. Thus, we aimed to evaluate the effect of single preoperative dose of oral melatonin or vitamin C administration on...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,随机对照试验
doi:10.17305/bjbms.2019.4379
更新日期:2020-02-05 00:00:00
abstract::After lung cancer colorectal cancer (Cc) is ranked the second, as a cause of cancer-related death. The purpose of this study was to analyze the Cc cases in our material with respect to all prognostic values including histological type and grade, vascular invasion, perineural invasion, and tumor border features. There ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2851
更新日期:2009-02-01 00:00:00
abstract::The aim of our study was to examine NPHS1, NPHS2, WT1 and LAMB2 mutations, previously reported in two thirds of patients with nephrotic syndrome with onset before the age of one year old. Genomic DNA samples from Polish children (n=33) with Steroid-Resistant Nephrotic Syndrome (SRNS) due to focal segmental glomerulosc...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2014.2270
更新日期:2014-05-01 00:00:00
abstract::Atherosclerosis is a pathological condition that begins in early childhood, but clinically the disease manifests in older age. The aim of work was to determine frequency of atherosclerosis risk factors in healthy school children. Cross-sectional study included 214 children in mean age 10,99+/-2,52 years, within range ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2803
更新日期:2009-08-01 00:00:00
abstract::Number of hemodialysis patients each day is increasing. The quality of their lives is largely determined by the quality of hemodialysis treatment. One of the most important factors is the type of applied blood approach. The type of blood approach in the most case is artery venous fistula, permanent, temporary catheter...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2009.2776
更新日期:2009-11-01 00:00:00
abstract::Innocent heart murmurs are hearth murmurs that occur in patients with a normal heart structure. They do not represent a disease of the heart and vascular system, and should not be treated as such. Iron-deficiency anaemia often causes, along with other symptoms, systolic heart murmurs and tachycardia. It appears in chi...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2005.3269
更新日期:2005-08-01 00:00:00
abstract::Tranexamic acid (TXA) reduces blood loss and transfusion rates in unilateral total knee arthroplasty (TKA), but there is limited data regarding its efficacy in bilateral TKA. This study reports the impact TXA has on clinical outcomes and hospital cost of care in simultaneous, primary bilateral TKA. The 449 patients we...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2020.5060
更新日期:2020-10-27 00:00:00
abstract::There is still debate whether sintethic graft (polytetrafluoroethylene or Dacron) is equivalent to vein as bypass graft material for the above-knee femoropopliteal bypass. Therefore, we performed prospective randomized trial to compare vein with polytetrafluoroethylene/dacron for femoropopliteal bypasses with the dist...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,随机对照试验
doi:10.17305/bjbms.2008.2899
更新日期:2008-11-01 00:00:00
abstract::Spermatogenesis is a tightly controlled, multi-step process in which mature spermatozoa are produced. Disruption of regulatory mechanisms in spermatogenesis can lead to male infertility, various diseases of male reproductive system, or even cancer. The spermatogenic impairment in infertile men can be associated with d...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:10.17305/bjbms.2018.3477
更新日期:2019-02-12 00:00:00
abstract::Status epilepticus (SE) is defined as continuous seizure activity lasting more than 5 minutes. It results in neuronal cell death, mediated by endoplasmic reticulum (ER) stress response. Previously, metformin demonstrated neuroprotective effects in primary cortical neurons. In this study, we analyzed the effect of metf...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2017.2044
更新日期:2018-02-20 00:00:00
abstract::Dishevelled family proteins (DVL1, DVL2, and DVL3) are cytoplasmic proteins that are involved in canonical and non-canonical Wnt signaling pathway during embryonic development. The role of DVL proteins in the placental tissue remains mostly unknown. In the current study, we explored the role of Dishevelled proteins in...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2020.5422
更新日期:2020-12-26 00:00:00
abstract::Nod-like receptor protein 3 (NLRP3) inflammasome has been implicated in alcoholic liver disease. Chronic alcohol consumption enhances gut permeability and causes microbial translocation. The present study explored the activation of the NLRP3 inflammasome by Escherichia coli RNA in hepatic stellate cells (HSCs), and th...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2016.699
更新日期:2016-01-14 00:00:00
abstract::Depression is among the most common of chronic health problems. WHO report predicts that depression will be the leading cause of disability in the industrial world by the year 2020. To be successful, treatment for the patients suffering from depression must be continued until complete recovery, but most patients do no...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2002.3575
更新日期:2002-12-01 00:00:00
abstract::This is the first study performed in population from Bosnia & Herzegovina (BH), in which we analysed a significance of genetic variations in drug-metabolising enzyme, cytochrome P450 (CYP), in pathogenesis of Type 2 diabetes. We have determined allele frequencies for CYP2C9*2, CYP2C19*2, and CYP2D6*4 in diabetic patie...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2010.2662
更新日期:2010-11-01 00:00:00
abstract::C-reactive protein (CRP) is an important predictor of cardiovascular events and plays a role in vascular inflammation and vessel damage. The aim of this study was to investigate the effect of pentameric CRP (pCRP) and monomeric CRP (mCRP) on the production of atherosclerosis-re-lated factors in cultured human coronary...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2020.4711
更新日期:2020-11-02 00:00:00
abstract::The search for bone reconstruction materials and methods is an ongoing challenge. The aim of this review is to systemically search the available literature concerning the clinical performance of eggshell as a substitute material in guided bone regeneration in oral surgery. Five databases (PubMed, Cochrane, Web of Scie...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:10.17305/bjbms.2020.4888
更新日期:2020-11-02 00:00:00
abstract::The new criteria in detection of ARVC/D produced more subtle noninvasive parameters that raised sensitivity in diagnosis. Since heart rate dynamics have prognostic significance for the progression of a disease and for mortality, the main objective was to explore its value in disclosing risk for serious arrhythmias. Ou...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2013.2419
更新日期:2013-02-01 00:00:00
abstract::Epileptic psychoses as the most complex psychopathological phenomena represent unexplored states for prognosis. Clinical trials, conveyed in order to found risk factors still are not consistent in their conclusions. By this research results of all biological, clinical, psychological and social, as well as demographic ...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2007.3069
更新日期:2007-05-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) are involved in a range of biological processes, such as cellular differentiation, migration, apoptosis, invasion, proliferation, and transcriptional regulation. The aberrant expression of lncRNAs plays a significant role in several cancer types. Aurora kinases are increasingly expressed...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2019.4155
更新日期:2020-02-05 00:00:00
abstract::The aim of this study was to assess echocardiographic changes in female patients with untreated dysfunctional thyroid states and whether the therapy aimed to normalize the thyroid dysfunction could lead to improvement in cardiac systolic and diastolic function. The study included 90 female subjects who performed contr...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2010.2704
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Carcinoembryonic antigen (CEA) is used as a tumour marker in breast cancer (BC). In order to assess diagnostic value of CEA in BC we examined its serum levels and frequencies of its increase in breast cancer patients (BCP), and compared them to those in controls. We also determined CEA in patient...
journal_title:Bosnian journal of basic medical sciences
pub_type: 杂志文章
doi:10.17305/bjbms.2003.3552
更新日期:2003-05-01 00:00:00