A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytes.

abstract：:There are two competing hypotheses for the origin of megaliths in Europe. The conventional view from the late 19th and early 20th centuries was of a single-source diffusion of megaliths in Europe from the Near East through the Mediterranean and along the Atlantic coast. Following early radiocarbon dating in the 1970s,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz Paulsson B

更新日期：2019-02-26 00:00:00

abstract：:Intact DNA molecules extracted from HSV-1 (herpes simplex virus 1, human herpes virus 1) strain MP virions have a molecular weight of approximately 97 X 10(6), but cleavage with the HinIII restriction enzyme yields fourteen fragments with summed molecular weights of 160 X 10(6). Six "major" fragments occur once in eve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hayward GS,Jacob RJ,Wadsworth SC,Roizman B

更新日期：1975-11-01 00:00:00

abstract：:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxin that causes parkinsonism in humans and nonhuman animals, and its use has led to greater understanding of the pathogenesis of Parkinson's disease. However, its molecular targets have not been defined. We show that mice lacking the gene for poly(ADP-rib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mandir AS,Przedborski S,Jackson-Lewis V,Wang ZQ,Simbulan-Rosenthal CM,Smulson ME,Hoffman BE,Guastella DB,Dawson VL,Dawson TM

更新日期：1999-05-11 00:00:00

abstract：:beta-Endorphin, an opiate-like peptide, has potent antinociceptive properties when it is administered directly into the brain and assayed in the the tail-flick, hot-plate, and writhing tests in mice and in the wet shake test in rats. On a molar basis, beta-endorphin is 18 to 33 times more potent than morphine and its ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loh HH,Tseng LF,Wei E,Li CH

更新日期：1976-08-01 00:00:00

abstract：:The sigma-N (sigmaN) subunit of the bacterial RNA polymerase is a sequence specific DNA-binding protein. The RNA polymerase holoenzyme formed with sigmaN binds to promoters in an inactive form and only initiates transcription when activated by enhancer-binding positive control proteins. We now provide evidence to show...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cannon WV,Chaney MK,Wang X,Buck M

更新日期：1997-05-13 00:00:00

abstract：:When various wild strains of Penicillium lanosum and Aspergillus niger were placed in the same mild laboratory environment, their frequencies of new spontaneous mutations were clearly related to whether they had been isolated from a region of high or low microclimatic stress. In the mild environment, the total frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamb BC,Mandaokar S,Bahsoun B,Grishkan I,Nevo E

更新日期：2008-04-15 00:00:00

abstract：:Sera from 30 patients with astrocytoma were tested for antibody reacting with cell surface antigens of cultured autologous astrocytoma cells. Ten percent of the patients had antibody detectable by mixed hemadsorption assays, approximately 50% by immune adherence and protein A assays, and 100% by anti-C3-mixed hemadsor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pfreundschuh M,Shiku H,Takahashi T,Ueda R,Ransohoff J,Oettgen HF,Old LJ

更新日期：1978-10-01 00:00:00

abstract：:Generating human single-nucleotide polymorphisms (SNPs) is no longer a rate-limiting step for genetic studies of disease. The number of SNPs in public databases already exceeds 200,000, and the total is expected to exceed 1,000,000 within a year. Rather, progress is limited by the inability to genotype large numbers o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirschhorn JN,Sklar P,Lindblad-Toh K,Lim YM,Ruiz-Gutierrez M,Bolk S,Langhorst B,Schaffner S,Winchester E,Lander ES

更新日期：2000-10-24 00:00:00

abstract：:The nusB5 mutant of Escherichia coli was originally selected for reduced ability to support the antitermination of transcription that is mediated by the gene N product of bacteriophage lambda. By analyzing pulse-labeled RNA with an RNA.DNA filter hybridization technique, we have shown that, in the nusB5 mutant, the ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharrock RA,Gourse RL,Nomura M

更新日期：1985-08-01 00:00:00

abstract：:Recently, p53 was demonstrated to affect the expression of the insulin-like growth factor 1 receptor (IGF-1R), a receptor tyrosine kinase that plays a crucial role in growth and survival of cancer cells. However, the underlying mechanisms for interaction between p53 and IGF-1R are still not fully understood. One of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Girnita L,Girnita A,Larsson O

更新日期：2003-07-08 00:00:00

abstract：:To identify proteins that regulate the transcriptional activity of c-Jun, we have used the yeast two-hybrid screen to detect mammalian polypeptides that might interact functionally with the N-terminal segment of c-Jun, a known regulatory region. Among the proteins identified is a short form of Stat3 (designated Stat3 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaefer TS,Sanders LK,Nathans D

更新日期：1995-09-26 00:00:00

abstract：:Membrane-mediated particle interactions depend both on the properties of the particles themselves and the membrane environment in which they are suspended. Experiments have shown that chiral rod-like inclusions dissolved in a colloidal membrane of opposite handedness assemble into colloidal rafts, which are finite-siz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller JM,Joshi C,Sharma P,Baskaran A,Baskaran A,Grason GM,Hagan MF,Dogic Z

更新日期：2019-08-06 00:00:00

abstract：:The development of protein subunit vaccines to combat some of the world's deadliest pathogens such as a malaria parasite, Plasmodium falciparum, is stalled, due in part to the inability to induce and sustain high-titer antibody responses. Here, we show the induction of persistent, high-titer antibody responses to reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Y,Przysiecki C,Flanagan E,Bello-Irizarry SN,Ionescu R,Muratova O,Dobrescu G,Lambert L,Keister D,Rippeon Y,Long CA,Shi L,Caulfield M,Shaw A,Saul A,Shiver J,Miller LH

更新日期：2006-11-28 00:00:00

abstract：:Stress-induced structural remodeling in the adult hippocampus, involving debranching and shortening of dendrites and suppression of neurogenesis, provides a cellular basis for understanding the impairment of neural plasticity in the human hippocampus in depressive illness. Accordingly, reversal of structural remodelin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Czéh B,Michaelis T,Watanabe T,Frahm J,de Biurrun G,van Kampen M,Bartolomucci A,Fuchs E

更新日期：2001-10-23 00:00:00

abstract：:A synthetic polymer has been prepared which contains dodecyl groups (to bind small substrate molecules) and methyleneimidazole side chains (as nucleophilic catalytic sites) linked to a polyethyleneimine framework. This macromolecule, with a high local concentration of binding and catalytic groups, catalyzes the hydrol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klotz IM,Royer GP,Scarpa IS

更新日期：1971-02-01 00:00:00

abstract：:The DEMETER (DME) DNA glycosylase initiates active DNA demethylation via the base-excision repair pathway and is vital for reproduction in Arabidopsis thaliana DME-mediated DNA demethylation is preferentially targeted to small, AT-rich, and nucleosome-depleted euchromatic transposable elements, influencing expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park JS,Frost JM,Park K,Ohr H,Park GT,Kim S,Eom H,Lee I,Brooks JS,Fischer RL,Choi Y

更新日期：2017-02-21 00:00:00

abstract：:Nucleotide excision repair (NER) prevents skin cancer by eliminating highly genotoxic cyclobutane pyrimidine dimers (CPDs) induced in DNA by the UVB component of sunlight. NER consists of two distinct but overlapping subpathways, i.e., global NER, which removes CPD from the genome overall, and transcription-coupled NE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mathonnet G,Leger C,Desnoyers J,Drouin R,Therrien JP,Drobetsky EA

更新日期：2003-06-10 00:00:00

abstract：:A randomized double-blind controlled trial was conducted to determine the efficacy of passive immunotherapy in the treatment of symptomatic human immunodeficiency virus (HIV) infection. This trial included 86 symptomatic patients randomized to receive plasma rich in anti-HIV-1 antibody or standard seronegative plasma....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vittecoq D,Chevret S,Morand-Joubert L,Heshmati F,Audat F,Bary M,Dusautoir T,Bismuth A,Viard JP,Barré-Sinoussi F

更新日期：1995-02-14 00:00:00

abstract：:Conventional "bulk" PCR often yields inefficient and nonuniform amplification of complex templates in DNA libraries, introducing unwanted biases. Amplification of single DNA molecules encapsulated in a myriad of emulsion droplets (emulsion PCR, ePCR) allows the mitigation of this problem. Different ePCR regimes were e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terekhov SS,Eliseev IE,Ovchinnikova LA,Kabilov MR,Prjibelski AD,Tupikin AE,Smirnov IV,Belogurov AA Jr,Severinov KV,Lomakin YA,Altman S,Gabibov AG

更新日期：2020-11-03 00:00:00

abstract：:Interactions between swimming cells and surfaces are essential to many microbiological processes, from bacterial biofilm formation to human fertilization. However, despite their fundamental importance, relatively little is known about the physical mechanisms that govern the scattering of flagellated or ciliated cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kantsler V,Dunkel J,Polin M,Goldstein RE

更新日期：2013-01-22 00:00:00

abstract：:The evolution of sensory systems has let mammals develop complicated tactile end organs to enable sophisticated sensory tasks, including social interaction, environmental exploration, and tactile discrimination. The Merkel disc, a main type of tactile end organ consisting of Merkel cells (MCs) and Aβ-afferent endings,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang W,Kanda H,Ikeda R,Ling J,DeBerry JJ,Gu JG

更新日期：2016-09-13 00:00:00

abstract：:Oligodeoxyribonucleotides with site-specific modifications have been used as substrates for Escherichia coli DNA polymerase I holoenzyme and Klenow fragment. Modifications included the bulky guanine-8-aminofluorene adduct and a guanine oxidation product resembling the product of photosensitized DNA oxidation. By a com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Connor D,Stöhrer G

更新日期：1985-04-01 00:00:00

abstract：:An oligoribonucleotide (a 27-mer) that mimics the sarcin/ricin (S/R) domain of Escherichia coli 23S rRNA binds elongation factor EF-G; the Kd is 6.9 microM, whereas for binding to ribosomes it is 0.7 microM. Binding saturates when EF-G and the S/R RNA are equimolar; at saturation 70% of the input RNA is in complexes w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Munishkin A,Wool IG

更新日期：1997-11-11 00:00:00

abstract：:While recent experiments revealed that some pioneer transcription factors (TFs) can bind to their target DNA sequences inside a nucleosome, the binding dynamics of their target recognitions are poorly understood. Here we used the latest coarse-grained models and molecular dynamics simulations to study the nucleosome-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan C,Takada S

更新日期：2020-08-25 00:00:00

abstract：:A sequence-specific single-stranded DNA-binding protein from the trypanosomatid protozoan Crithidia fasciculata binds to a sequence of 12 nucleotides located at the origin of replication of kinetoplast DNA minicircles. This sequence, termed the universal minicircle sequence (UMS), is conserved in the kinetoplast DNA m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzfati Y,Abeliovich H,Kapeller I,Shlomai J

更新日期：1992-08-01 00:00:00

abstract：:Ectopic activation of the Wnt signaling pathway is highly oncogenic for many human tissues. Here, we show that ectopic Wnt signaling increases the effective stem cell activity in mouse mammary glands in vivo. Furthermore, Wnt effectors induce the accumulation of mouse mammary epithelial progenitors (assayed by Hoechst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu BY,McDermott SP,Khwaja SS,Alexander CM

更新日期：2004-03-23 00:00:00

abstract：:Bacteria use membrane-integral sensor histidine kinases (HK) to perceive stimuli and transduce signals from the environment to the cytosol. Information on how the signal is transmitted across the membrane by HKs is still scarce. Combining both liquid- and solid-state NMR, we demonstrate that structural rearrangements ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salvi M,Schomburg B,Giller K,Graf S,Unden G,Becker S,Lange A,Griesinger C

更新日期：2017-03-21 00:00:00

abstract：:Origins of DNA replication in Schizosaccharomyces pombe lack a specific consensus sequence analogous to the Saccharomyces cerevisiae autonomously replicating sequence (ARS) consensus, raising the question of how they are recognized by the replication machinery. Because all well characterized S. pombe origins are locat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai J,Chuang RY,Kelly TJ

更新日期：2005-01-11 00:00:00

abstract：:Visual receptive fields (RFs) were mapped inside and outside the cortical representation of the optic disk in the striate cortex (area V1) of anesthetized and paralyzed Cebus monkeys. Unexpectedly, most cells were found to be binocularly driven, and the RFs mapped with contralateral-eye stimulation progressed in a top...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fiorani Júnior M,Rosa MG,Gattass R,Rocha-Miranda CE

更新日期：1992-09-15 00:00:00

abstract：:Functional reconstitution of membrane proteins within lipid bilayers is crucial for understanding their biological function in living cells. While this strategy has been extensively used with liposomes, reconstitution of membrane proteins in lipidic cubic mesophases presents significant challenges related to the struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Speziale C,Salvati Manni L,Manatschal C,Landau EM,Mezzenga R

更新日期：2016-07-05 00:00:00