Glutamate synthase MoGlt1-mediated glutamate homeostasis is important for autophagy, virulence and conidiation in the rice blast fungus.

Abstract:

:Glutamate homeostasis plays a vital role in central nitrogen metabolism and coordinates several key metabolic functions. However, its function in fungal pathogenesis and development has not been investigated in detail. In this study, we identified and characterized a glutamate synthase gene MoGLT1 in the rice blast fungus Magnaporthe oryzae that was important to glutamate homeostasis. MoGLT1 was constitutively expressed, but showed the highest expression level in appressoria. Deletion of MoGLT1 resulted in a significant reduction in conidiation and virulence. The ΔMoglt1 mutants were defective in appressorial penetration and the differentiation and spread of invasive hyphae in penetrated plant cells. The addition of exogenous glutamic acid partially rescued the defects of the ΔMoglt1 mutants in conidiation and plant infection. Assays for MoAtg8 expression and localization showed that the ΔMoglt1 mutants were defective in autophagy. The ΔMoglt1 mutants were delayed in the mobilization of glycogens and lipid bodies from conidia to developing appressoria. Taken together, our results show that glutamate synthase MoGlt1-mediated glutamate homeostasis is important for pathogenesis and development in the rice blast fungus, possibly via the regulation of autophagy.

journal_name

Mol Plant Pathol

authors

Zhou W,Shi W,Xu XW,Li ZG,Yin CF,Peng JB,Pan S,Chen XL,Zhao WS,Zhang Y,Yang J,Peng YL

doi

10.1111/mpp.12541

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

564-578

issue

3

eissn

1464-6722

issn

1364-3703

journal_volume

19

pub_type

杂志文章
  • Sugar homeostasis mediated by cell wall invertase GRAIN INCOMPLETE FILLING 1 (GIF1) plays a role in pre-existing and induced defence in rice.

    abstract::Sugar metabolism and sugar signalling are not only critical for plant growth and development, but are also important for stress responses. However, how sugar homeostasis is involved in plant defence against pathogen attack in the model crop rice remains largely unknown. In this study, we observed that the grains of gi...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12078

    authors: Sun L,Yang DL,Kong Y,Chen Y,Li XZ,Zeng LJ,Li Q,Wang ET,He ZH

    更新日期:2014-02-01 00:00:00

  • Green leaf volatiles and jasmonic acid enhance susceptibility to anthracnose diseases caused by Colletotrichum graminicola in maize.

    abstract::Colletotrichum graminicola is a hemibiotrophic fungus that causes anthracnose leaf blight (ALB) and anthracnose stalk rot (ASR) in maize. Despite substantial economic losses caused by these diseases, the defence mechanisms against this pathogen remain poorly understood. Several hormones are suggested to aid in defence...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12924

    authors: Gorman Z,Christensen SA,Yan Y,He Y,Borrego E,Kolomiets MV

    更新日期:2020-05-01 00:00:00

  • The siderophore biosynthetic gene SID1, but not the ferroxidase gene FET3, is required for full Fusarium graminearum virulence.

    abstract:SUMMARY:To acquire iron from plant hosts, fungal pathogens have evolved at least two pathways for iron uptake. One system is hinged on the secretion and subsequent uptake of low-molecular-weight iron chelators termed siderophores, while the other uses cell-surface reductases to solubilize ferric iron by reducing it to ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00401.x

    authors: Greenshields DL,Liu G,Feng J,Selvaraj G,Wei Y

    更新日期:2007-07-01 00:00:00

  • Abscisic acid deficiency leads to rapid activation of tomato defence responses upon infection with Erwinia chrysanthemi.

    abstract::In addition to the important role of abscisic acid (ABA) in abiotic stress signalling, basal and high ABA levels appear to have a negative effect on disease resistance. Using the ABA-deficient sitiens tomato (Solanum lycopersicum) mutant and different application methods of exogenous ABA, we demonstrated the influence...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00437.x

    authors: Asselbergh B,Achuo AE,Höfte M,Van Gijsegem F

    更新日期:2008-01-01 00:00:00

  • Involvement of salicylic acid, ethylene and jasmonic acid signalling pathways in the susceptibility of tomato to Fusarium oxysporum.

    abstract::Phytohormones, such as salicylic acid (SA), ethylene (ET) and jasmonic acid (JA), play key roles in plant defence following pathogen attack. The involvement of these hormones in susceptibility following Fusarium oxysporum (Fo) infection has mostly been studied in Arabidopsis thaliana. However, Fo causes vascular wilt ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12559

    authors: Di X,Gomila J,Takken FLW

    更新日期:2017-09-01 00:00:00

  • Scab resistance in 'Geneva' apple is conditioned by a resistance gene cluster with complex genetic control.

    abstract::Apple scab, caused by the fungal pathogen Venturia inaequalis, is one of the most severe diseases of apple worldwide. It is the most studied plant-pathogen interaction involving a woody species using modern genetic, genomic, proteomic and bioinformatic approaches in both species. Although 'Geneva' apple was recognized...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12269

    authors: Bastiaanse H,Bassett HC,Kirk C,Gardiner SE,Deng C,Groenworld R,Chagné D,Bus VG

    更新日期:2016-02-01 00:00:00

  • The PCR amplification and characterization of entire Pseudomonas syringae hrp/hrc clusters.

    abstract::SUMMARY We describe the use of degenerate primers to amplify the core hrp/hrc region of P. syringae pv. phaseolicola NPS3121. 18 826 bp were amplified using long-range PCR, digested and shotgun sequenced. Sequence analysis finds a hypervariable region between the hrpU and hrpC operons, and indications of positive sele...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2004.00212.x

    authors: Gropp SJ,Guttman DS

    更新日期:2004-03-01 00:00:00

  • Structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode Globodera rostochiensis.

    abstract::SUMMARY The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expres...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00394.x

    authors: Kudla U,Milac AL,Qin L,Overmars H,Roze E,Holterman M,Petrescu AJ,Goverse A,Bakker J,Helder J,Smant G

    更新日期:2007-05-01 00:00:00

  • Functional analysis of an extracellular catalase of Botrytis cinerea.

    abstract::Summary There is evidence that the necrotrophic fungal pathogen Botrytis cinerea is exposed to oxidative processes within plant tissues. The pathogen itself also generates active oxygen species and H(2)O(2) as pathogenicity factors. Our aim was to study how the pathogen may defend itself against cellular damage caused...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2002.00114.x

    authors: Schouten A,Tenberge KB,Vermeer J,Stewart J,Wagemakers L,Williamson B,van Kan JA

    更新日期:2002-07-01 00:00:00

  • The tobacco Ntann12 gene, encoding an annexin, is induced upon Rhodoccocus fascians infection and during leafy gall development.

    abstract::SUMMARY Annexins are calcium-binding proteins that have been associated in plants with different biological processes such as responses to abiotic stress and early nodulation stages. Until now, the implication of annexins during plant-pathogen interactions has not been reported. Here, a novel plant annexin gene induce...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2007.00385.x

    authors: Vandeputte O,Lowe YO,Burssens S,VAN Raemdonck D,Hutin D,Boniver D,Geelen D,El Jaziri M,Baucher M

    更新日期:2007-03-01 00:00:00

  • Chloroplast-associated metabolic functions influence the susceptibility of maize to Ustilago maydis.

    abstract::Biotrophic fungal pathogens must evade or suppress plant defence responses to establish a compatible interaction in living host tissue. In addition, metabolic changes during disease reflect both the impact of nutrient acquisition by the fungus to support proliferation and the integration of metabolism with the plant d...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12485

    authors: Kretschmer M,Croll D,Kronstad JW

    更新日期:2017-12-01 00:00:00

  • Microbe-associated molecular pattern (MAMP) signatures, synergy, size and charge: influences on perception or mobility and host defence responses.

    abstract::Triggering of defences by microbes has mainly been investigated using single elicitors or microbe-associated molecular patterns (MAMPs), but MAMPs are released in planta as complex mixtures together with endogenous oligogalacturonan (OGA) elicitor. We investigated the early responses in Arabidopsis of calcium influx a...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2009.00537.x

    authors: Aslam SN,Erbs G,Morrissey KL,Newman MA,Chinchilla D,Boller T,Molinaro A,Jackson RW,Cooper RM

    更新日期:2009-05-01 00:00:00

  • Detecting and differentiating Acremonium implicatum: developing a PCR-based method for an endophytic fungus associated with the genus Brachiaria.

    abstract::SUMMARY Brachiaria is a pan-tropical genus of grasses with about 100 species. The fungus Acremonium implicatum can develop an endophytic association that is mutually beneficial with Brachiaria species. We developed a polymerase chain reaction (PCR)-based method by first amplifying DNA from A. implicatum isolates using...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2003.00157.x

    authors: Kelemu S,Dongyi H,Guixiu H,Takayama Y

    更新日期:2003-03-01 00:00:00

  • Bacterial and plant natriuretic peptides improve plant defence responses against pathogens.

    abstract::Plant natriuretic peptides (PNPs) have been implicated in the regulation of ions and water homeostasis, and their participation in the plant immune response has also been proposed. Xanthomonas citri ssp. citri contains a gene encoding a PNP-like protein (XacPNP) which has no homologues in other bacteria. XacPNP mimics...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12560

    authors: Ficarra FA,Grandellis C,Garavaglia BS,Gottig N,Ottado J

    更新日期:2018-04-01 00:00:00

  • The Botrytis cinerea cerato-platanin BcSpl1 is a potent inducer of systemic acquired resistance (SAR) in tobacco and generates a wave of salicylic acid expanding from the site of application.

    abstract::Systemic acquired resistance (SAR) is a potent plant defence system that, in response to a first contact with a plant pathogen, prepares the whole plant for subsequent attacks, so that it becomes more resistant to the same and to other pathogens. BcSpl1, a cerato-platanin family protein abundantly secreted by Botrytis...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2012.00842.x

    authors: Frías M,Brito N,González C

    更新日期:2013-02-01 00:00:00

  • Surface polysaccharides and quorum sensing are involved in the attachment and survival of Xanthomonas albilineans on sugarcane leaves.

    abstract::Xanthomonas albilineans, the causal agent of sugarcane leaf scald, is a bacterial plant pathogen that is mainly spread by infected cuttings and contaminated harvesting tools. However, some strains of this pathogen are known to be spread by aerial means and are able to colonize the phyllosphere of sugarcane before ente...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12276

    authors: Mensi I,Daugrois JH,Pieretti I,Gargani D,Fleites LA,Noell J,Bonnot F,Gabriel DW,Rott P

    更新日期:2016-02-01 00:00:00

  • Olive anthracnose: a yield- and oil quality-degrading disease caused by several species of Colletotrichum that differ in virulence, host preference and geographical distribution.

    abstract::Olive anthracnose causes fruit rot leading to its drop or mummification, resulting in yield losses and the degradation of oil quality. TAXONOMY AND DISTRIBUTION:The disease is caused by diverse species of Colletotrichum, mostly clustering in the C. acutatum species complex. Colletotrichum nymphaeae and C. godetiae ar...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12676

    authors: Talhinhas P,Loureiro A,Oliveira H

    更新日期:2018-03-08 00:00:00

  • Pathogenic fungi: leading or led by ambient pH?

    abstract::SUMMARY Pathogenic fungi have successfully attacked a wide range of hosts, which has forced them into ambient-adaptation. pH is one of the major ambient traits affecting the activity of pathogenicity factors secreted by the pathogen, hence, a pH sensing-response system was developed to enable the pathogen to tailor it...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1046/j.1364-3703.2003.00196.x

    authors: Prusky D,Yakoby N

    更新日期:2003-11-01 00:00:00

  • Signalling requirements for Erwinia amylovora-induced disease resistance, callose deposition and cell growth in the non-host Arabidopsis thaliana.

    abstract::Erwinia amylovora is the causal agent of the fire blight disease in some plants of the Rosaceae family. The non-host plant Arabidopsis serves as a powerful system for the dissection of mechanisms of resistance to E. amylovora. Although not yet known to mount gene-for-gene resistance to E. amylovora, we found that Arab...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12588

    authors: Hamdoun S,Gao M,Gill M,Kwon A,Norelli JL,Lu H

    更新日期:2018-05-01 00:00:00

  • Mutual interplay between phytopathogenic powdery mildew fungi and other microorganisms.

    abstract::Powdery mildew is a common and widespread plant disease of considerable agronomic relevance. It is caused by obligate biotrophic fungal pathogens which, in most cases, epiphytically colonize aboveground plant tissues. The disease has been typically studied as a binary interaction of the fungal pathogen with its plant ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/mpp.12771

    authors: Panstruga R,Kuhn H

    更新日期:2019-04-01 00:00:00

  • The synthetic cationic lipid diC14 activates a sector of the Arabidopsis defence network requiring endogenous signalling components.

    abstract::Natural and synthetic elicitors have contributed significantly to the study of plant immunity. Pathogen-derived proteins and carbohydrates that bind to immune receptors, allow the fine dissection of certain defence pathways. Lipids of a different nature that act as defence elicitors, have also been studied, but their ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12252

    authors: Cambiagno DA,Lonez C,Ruysschaert JM,Alvarez ME

    更新日期:2015-12-01 00:00:00

  • Analysis of polymorphism and transcription of the effector gene Avr1b in Phytophthora sojae isolates from China virulent to Rps1b.

    abstract::The effector gene Avr1b-1 of Phytophthora sojae determines the efficacy of the resistance gene Rps1b in soybean. The sequences of the Avr1b-1 locus in 34 Chinese isolates of P. sojae were obtained and analysed by polymerase chain reaction (PCR) and inverse PCR. Four different alleles and a complete deletion mutation o...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2011.00733.x

    authors: Cui L,Yin W,Dong S,Wang Y

    更新日期:2012-02-01 00:00:00

  • A functionally conserved Zn2 Cys6 binuclear cluster transcription factor class regulates necrotrophic effector gene expression and host-specific virulence of two major Pleosporales fungal pathogens of wheat.

    abstract::The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch of wheat (Triticum aestivum). The interaction is mediated by multiple fungal necrotrophic effector-dominant host sensitivity gene interactions. The three best-characterized effector-sensitivity gene systems are SnToxA-Tsn1, SnTox1-Snn1 ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12511

    authors: Rybak K,See PT,Phan HT,Syme RA,Moffat CS,Oliver RP,Tan KC

    更新日期:2017-04-01 00:00:00

  • TfmR, a novel TetR-family transcriptional regulator, modulates the virulence of Xanthomonas citri in response to fatty acids.

    abstract::The type III secretion system (T3SS) is required for Xanthomonas citri subsp. citri (Xcc) virulence by translocating effectors into host cytoplasm to promote disease development. The T3SS is controlled by the master transcriptional regulators HrpG and HrpX. While the function of HrpG and HrpX are well characterized, t...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12786

    authors: Teper D,Zhang Y,Wang N

    更新日期:2019-05-01 00:00:00

  • Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range.

    abstract:TAXONOMY:Soybean mosaic virus (SMV) is a species within the genus Potyvirus, family Potyviridae, which includes almost one-quarter of all known plant RNA viruses affecting agriculturally important plants. The Potyvirus genus is the largest of all genera of plant RNA viruses with 160 species. PARTICLE:The filamentous p...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12644

    authors: Hajimorad MR,Domier LL,Tolin SA,Whitham SA,Saghai Maroof MA

    更新日期:2018-07-01 00:00:00

  • Chloroplast clustering around the nucleus is a general response to pathogen perception in Nicotiana benthamiana.

    abstract::It is increasingly clear that chloroplasts play a central role in plant stress responses. Upon activation of immune responses, chloroplasts are the source of multiple defensive signals, including reactive oxygen species (ROS). Intriguingly, it has been described that chloroplasts establish physical contact with the nu...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12840

    authors: Ding X,Jimenez-Gongora T,Krenz B,Lozano-Duran R

    更新日期:2019-09-01 00:00:00

  • Horizontal gene transfer: sustaining pathogenicity and optimizing host-pathogen interactions.

    abstract::Successful host-pathogen interactions require the presence, maintenance and expression of gene cassettes called 'pathogenicity islands' (PAIs) and 'metabolic islands' (MAIs) in the respective pathogen. The products of these genes confer on the pathogen the means to recognize their host(s) and to efficiently evade host...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1364-3703.2008.00518.x

    authors: Kado CI

    更新日期:2009-01-01 00:00:00

  • Moniliophthora roreri, causal agent of cacao frosty pod rot.

    abstract::Taxonomy: Moniliophthora roreri (Cif.) H.C. Evans et al. ; Phylum Basidiomycota; Class Agaricomycetes; Order Agaricales; Family Marasmiaceae; Genus Moniliophthora. Biology: Moniliophthora roreri attacks Theobroma and Herrania species causing frosty pod rot. Theobroma cacao (cacao) is the host of major economic concern...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12648

    authors: Bailey BA,Evans HC,Phillips-Mora W,Ali SS,Meinhardt LW

    更新日期:2018-07-01 00:00:00

  • Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1.

    abstract::SUMMARY The Bipolaris sorokiniana tolerant 1 (bst1) barley mutant is derived from fast neutron-irradiated seeds of wild-type Bowman(Rph3). The induced mutation was genetically localized to a position on chromosome 5HL distal to the centromere using amplified fragment length polymorphism markers. In addition, the defen...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/j.1364-3703.2009.00555.x

    authors: Persson M,Falk A,Dixelius C

    更新日期:2009-09-01 00:00:00

  • Silencing PsKPP4, a MAP kinase kinase kinase gene, reduces pathogenicity of the stripe rust fungus.

    abstract::Many obligately parasitic pathogens absorb nutrients from host plants via specialized infection structures, called haustoria and infection hyphae, to further colonization and growth in the host plant. In the wheat (Triticum aestivum) stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), the mitogen-activated ...

    journal_title:Molecular plant pathology

    pub_type: 杂志文章

    doi:10.1111/mpp.12731

    authors: Zhu X,Guo J,He F,Zhang Y,Tan C,Yang Q,Huang C,Kang Z,Guo J

    更新日期:2018-12-01 00:00:00