Abstract:
:We investigated the cytotoxic interactions of romidepsin, a histone deacetylase inhibitor, and lenalidomide, an immunomodulatory agent, in a T-cell lymphoma preclinical model. Hut-78 and Karpas-299 cells were treated with romidepsin and lenalidomide alone and in combination. The interaction between romidepsin and lenalidomide was evaluated by the Chou-Talalay method, and cell viability and clonogenicity were also evaluated. Apoptosis, reactive oxygen species (ROS) levels, and cell cycle distribution were determined by flow cytometry. ER stress, caspase activation, and the AKT, MAPK/ERK, and STAT-3 pathways were analyzed by Western blot. Combination treatment with romidepsin and lenalidomide had a synergistic effect in Hut-78 cells and an additive effect in Karpas-299 cells at 24 hours and did not decrease the viability of normal peripheral blood mononuclear cells. This drug combination induced apoptosis, increased ROS production, and activated caspase-8, -9, -3 and PARP. Apoptosis was associated with increased hallmarks of ER stress and activation of UPR sensors and was mediated by dephosphorylation of the AKT, MAPK/ERK, and STAT3 pathways.The combination of romidepsin and lenalidomide shows promise as a possible treatment for T-cell lymphoma. This work provides a basis for further studies.
journal_name
Cancer Biol Therjournal_title
Cancer biology & therapyauthors
Cosenza M,Civallero M,Fiorcari S,Pozzi S,Marcheselli L,Bari A,Ferri P,Sacchi Sdoi
10.1080/15384047.2016.1219820subject
Has Abstractpub_date
2016-10-02 00:00:00pages
1094-1106issue
10eissn
1538-4047issn
1555-8576journal_volume
17pub_type
杂志文章abstract::Bruton's tyrosine kinase (BTK) is a non-receptor tyrosine kinase that has mainly been studied in haematopoietic cells. We have investigated whether BTK is a potential therapeutic target in prostate cancer. We find that BTK is expressed in prostate cells, with the alternate BTK-C isoform predominantly expressed in pros...
journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.1080/15384047.2015.1078023
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journal_title:Cancer biology & therapy
pub_type: 杂志文章,评审
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journal_title:Cancer biology & therapy
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doi:10.4161/cbt.8.20.9804
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journal_title:Cancer biology & therapy
pub_type: 杂志文章,评审
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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更新日期:2007-03-01 00:00:00
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.20840
更新日期:2012-08-01 00:00:00
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/15384047.2014.955992
更新日期:2014-01-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.24599
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.10.6.12532
更新日期:2010-09-15 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.1080/15384047.2015.1070988
更新日期:2015-01-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.1080/15384047.2016.1139266
更新日期:2016-04-02 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.8.19.9650
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journal_title:Cancer biology & therapy
pub_type: 杂志文章,多中心研究
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.9.5.10887
更新日期:2010-03-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.4.10.2022
更新日期:2005-10-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.4.12.2183
更新日期:2005-12-01 00:00:00
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journal_title:Cancer biology & therapy
pub_type: 杂志文章
doi:10.4161/cbt.12.3.15949
更新日期:2011-08-01 00:00:00