A novel bispecific antibody, BiSS, with potent anti-cancer activities.

Abstract:

:One of the most active fields in cancer immunotherapy is the study of bispecific antibodies, which engage immune cells to kill cancer cells. However, a variety of issues are associated with most of current bispecific antibody formats. In this study, we present a novel bispecific antibody, BiSS (Bispecific antibody with Single domain, Single domain antibodies), which was constructed by linking 2 single domain antibodies, anti-CEA and anti-CD16, in tandem. Unlike most other bispecific antibodies, the BiSS antibody can be expressed and purified from E.coli in large quantities. By recruiting natural killer cells (NK cells) to CEA-positive cancer cells, BiSS led to cancer cell death in vitro. In xenograft models, the BiSS protein blocked cancer progression. The data suggested that the single domain-based bispecific antibody BiSS was functional and can be potentially applied to a broad range of immunotherapies.

journal_name

Cancer Biol Ther

journal_title

Cancer biology & therapy

authors

Dong B,Zhou C,He P,Li J,Chen S,Miao J,Li Q,Wang Z

doi

10.1080/15384047.2016.1139266

subject

Has Abstract

pub_date

2016-04-02 00:00:00

pages

364-70

issue

4

eissn

1538-4047

issn

1555-8576

journal_volume

17

pub_type

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