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Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte.

Abstract：

OBJECTIVE:To quantify cytoplasmic maturity on the basis of intracytoplasmic sperm injection (ICSI) injection funnel manifestation and to evaluate influence factors of the latter. DESIGN:Prospective study. SETTING:Private fertility center. PATIENT(S):A total of 31 patients with good ovarian response. INTERVENTION(S):Mature and immature oocytes were injected intracytoplasmatically. Formation and persistence of an injection funnel was documented and measured. MAIN OUTCOME MEASURE(S):ICSI funnel size, persistence of injection funnel, rates of degeneration and fertilization, embryo quality. RESULT(S):Funnel volume in germinal vesicle stage oocytes (prophase I [PI]) was significantly smaller than that of metaphase I (MI) and MII oocytes. Immature eggs (PI, MI) almost never showed a persistent funnel 2-4 minutes after ICSI, whereas in MII eggs the funnel was still observable in 35% (117/334) of the cases. Uni- and multivariate analysis revealed that pipette type and stimulation protocol significantly influenced appearance of injection funnel. Funnel volume in oocytes that fertilized regularly was significantly higher compared with three-polar body and degenerated oocytes. CONCLUSION(S):Oocyte maturation within the follicle is closely associated with a remarkable change in cytoplasm viscosity from an aqueous to a more viscous subtype. Precise evaluation of the injection funnel may help to explain deviations from expected ICSI outcome and could also assist in optimizing controlled ovarian hyperstimulation.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Krause I,Pohler U,Grosse S,Shebl O,Petek E,Chandra A,Ebner T

doi

10.1016/j.fertnstert.2016.06.015

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

1101-1106

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)61344-4

journal_volume

106

pub_type

杂志文章

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