当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF PHARMACEUTICS期刊下所有文献 > Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro.

Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro.

Abstract：

:Development of novel anticancer formulations is a priority challenge in biomedicine. However, in vitro models based on monolayer cultures (2D) which are currently used for cytotoxicity tests leave much to be desired. More and more attention is focusing on 3D in vitro systems which can better mimic solid tumors. The aim of the study was to develop a novel one-step highly reproducible technique for multicellular tumor spheroid (MTS) formation using synthetic cyclic RGD-peptides, and to demonstrate availability of the spheroids as 3D in vitro model for antitumor drug testing. Cell self-assembly effect induced by addition of both linear and cyclic RGD-peptides directly to monolayer cultures was studied for 12 cell lines of various origins, including tumor cells (e.i. U-87 MG, MCF-7, M-3, HCT-116) and normal cells, in particular L-929, BNL.CL2, HepG2. Cyclo-RGDfK and its modification with triphenylphosphonium cation (TPP), namely cyclo-RGDfK(TPP) in a range of 10-100μM were found to induce spheroid formation. The obtained spheroids were unimodal with mean sizes in a range of 60-120μm depending on cell line and serum content in culture medium. The spheroids were used as 3D in vitro model, in order to evaluate cytotoxicity effects of antitumor drugs (doxorubicin, curcumin, temozolomide). The developed technique could be proposed as a promising tool for in vitro test of novel antitumor drugs.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Akasov R,Zaytseva-Zotova D,Burov S,Leko M,Dontenwill M,Chiper M,Vandamme T,Markvicheva E

doi

10.1016/j.ijpharm.2016.04.005

subject

Has Abstract

pub_date

2016-06-15 00:00:00

pages

148-57

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378-5173(16)30280-0

journal_volume

506

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF PHARMACEUTICS文献大全
  • Using thin film freezing to minimize excipients in inhalable tacrolimus dry powder formulations.

    abstract：:We investigated the feasibility of preparing high-potency tacrolimus dry powder for inhalation using thin film freezing (TFF). We found that using ultra-rapid freezing can increase drug loading up to 95% while maintaining good aerosol performance. Drug loading affected the specific surface area and moisture sorption o...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119490

    authors： Sahakijpijarn S,Moon C,Ma X,Su Y,Koleng JJ,Dolocan A,Williams RO 3rd

    更新日期：2020-08-30 00:00:00

  • Predicting the solubility-permeability interplay when using cyclodextrins in solubility-enabling formulations: model validation.

    abstract：:Although the extraordinary solubility advantage afforded by cyclodextrins has led to their widespread use as pharmaceutical solubilizers, several reports have emerged that cyclodextrins may also reduce the apparent permeability of the drug. With the purpose to investigate this solubility-permeability interplay, we hav...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.03.017

    authors： Miller JM,Dahan A

    更新日期：2012-07-01 00:00:00

  • Stereolithographic (SLA) 3D printing of oral modified-release dosage forms.

    abstract：:The aim of this work was to evaluate the suitability of stereolithography (SLA) to fabricate drug-loaded tablets with modified-release characteristics. The SLA printer creates solid objects by using a laser beam to photopolymerise monomers. In this work polyethylene glycol diacrylate (PEGDA) was used as a monomer and ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2016.03.016

    authors： Wang J,Goyanes A,Gaisford S,Basit AW

    更新日期：2016-04-30 00:00:00

  • General procedure to aid the development of continuous pharmaceutical processes using multivariate statistical modeling - an industrial case study.

    abstract：:Streamlining the manufacturing process has been recognized as a key issue to reduce production costs and improve safety in pharmaceutical manufacturing. Although data available from earlier developmental stages are often sparse and unstructured, they can be very useful to improve the understanding about the process un...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2013.01.018

    authors： Tomba E,De Martin M,Facco P,Robertson J,Zomer S,Bezzo F,Barolo M

    更新日期：2013-02-28 00:00:00

  • Synbiotic loaded chitosan-Ca-alginate microparticles reduces inflammation in the TNBS model of rat colitis.

    abstract：:New therapeutic strategies against inflammatory bowel disease (IBD) consider the usage of probiotics, prebiotics and synbiotics as beneficial for the intestinal microbial balance. Limitations of such an approach are addressed into difference in survival, persistence, colonization and variable effects among different p...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.05.049

    authors： Ivanovska TP,Mladenovska K,Zhivikj Z,Pavlova MJ,Gjurovski I,Ristoski T,Petrushevska-Tozi L

    更新日期：2017-07-15 00:00:00

  • Interactions between a poorly soluble cationic drug and sodium dodecyl sulfate in dissolution medium and their impact on in vitro dissolution behavior.

    abstract：:In the pharmaceutical industry, in vitro dissolution testing ofsolid oral dosage forms is a very important tool for drug development and quality control. However, ion-pairing interaction between the ionic drugand surfactants in dissolution medium often occurs, resulting in inconsistent and incomplete drug release. The...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.10.063

    authors： Huang Z,Parikh S,Fish WP

    更新日期：2018-01-15 00:00:00

  • Innovative pMDI formulations of spray-dried nanoparticles for efficient pulmonary drug delivery.

    abstract：:For drug delivery to the lungs, the aerodynamic size of drug particles plays a predominant role in determining the sites of deposition in the airway, and the particles with the size less than 2μm are highly expected as they will be preferably delivered to the ideal site of alveolar regions. In this paper, a novel plat...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.07.040

    authors： Li HY,Xu EY

    更新日期：2017-09-15 00:00:00

  • Amorphous drug dispersions with mono- and diacyl lecithin: On molecular categorization of their feasibility and UV dissolution imaging.

    abstract：:There is a growing interest in drug-phospholipid complexes and similar formulations that are mostly solid dispersions with high drug load. This study aims to explore the feasibility of such phospholipid-based solid dispersions as well as to characterize them. A particular aim was to compare monoacyl phosphatidylcholin...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.06.039

    authors： Gautschi N,Van Hoogevest P,Kuentz M

    更新日期：2015-08-01 00:00:00

  • Impact of ring size and drug loading on the pharmacokinetics of a combination dapivirine-darunavir vaginal ring in cynomolgus macaques.

    abstract：:This work investigates the impact of vaginal ring size and drug loading on the in vitro release, safety, ease of fit, and pharmacokinetics in cynomolgus macaques of matrix-type silicone elastomer vaginal rings containing a combination of the non-nucleoside reverse transcriptase inhibitor dapivirine and the protease in...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.08.051

    authors： Murphy DJ,Desjardins D,Boyd P,Dereuddre-Bosquet N,Stimmer L,Caldwell A,Le Grand R,Kelly C,van Roey J,Malcolm RK

    更新日期：2018-10-25 00:00:00

  • Gene transfer by histidylated lipopolyplexes: A dehydration method allowing preservation of their physicochemical parameters and transfection efficiency.

    abstract：:Lipid-Polycation-DNA complexes (LPD) is a promising non-viral system for nucleic acids delivery. Usually, LPD are prepared just before their use. In the present work, we have examined whether dehydration of a new type of LPD (named LPD100) might be a storage option. LPD100 comprises PEGylated histidylated polylysine/p...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.04.009

    authors： Perche F,Lambert O,Berchel M,Jaffrès PA,Pichon C,Midoux P

    更新日期：2012-02-14 00:00:00

  • "Sponge-like" dressings based on biopolymers for the delivery of platelet lysate to skin chronic wounds.

    abstract：:The aim of the present work was the development of sponge-like dressings, obtained by freeze-drying, based on chitosan glutamate and sodium hyaluronate for platelet lysate (PL) delivery to chronic skin wounds. A first phase of the research focused on the choice of the best dressing composition to obtain formulations e...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.07.056

    authors： Rossi S,Faccendini A,Bonferoni MC,Ferrari F,Sandri G,Del Fante C,Perotti C,Caramella CM

    更新日期：2013-01-20 00:00:00

  • Gastroretentive drug delivery system of DA-6034, a new flavonoid derivative, for the treatment of gastritis.

    abstract：:A gastroretentive drug delivery system of DA-6034, a new synthetic flavonoid derivative, for the treatment of gastritis was developed by using effervescent floating matrix system (EFMS). The therapeutic limitations of DA-6034 caused by its low solubility in acidic conditions were overcome by using the EFMS, which was ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2007.12.042

    authors： Jang SW,Lee JW,Park SH,Kim JH,Yoo M,Na DH,Lee KC

    更新日期：2008-05-22 00:00:00

  • Tumor selectivity of stealth multi-functionalized superparamagnetic iron oxide nanoparticles.

    abstract：:Superparamagnetic iron oxide nanoparticles (SPIO-NPs) have traditionally been used as MRI contrast agent for disease imaging via passive targeting. However, there has been an increasing interest in the development of SPIO-NPs to cellular-specific targeting for imaging and drug delivery currently. The objective of our ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.10.038

    authors： Fan C,Gao W,Chen Z,Fan H,Li M,Deng F,Chen Z

    更新日期：2011-02-14 00:00:00

  • Effects of polyamidoamine (PAMAM) dendrimers on the nasal absorption of poorly absorbable drugs in rats.

    abstract：:The absorption enhancing effects of polyamidoamine (PAMAM) dendrimers with various concentrations and generations on the nasal absorption of fluorescein isothiocyanate-labeled dextran with an average molecular weight of 4400 (FD4) were initially studied in rats. PAMAM dendrimers with different generations improved the...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.04.021

    authors： Dong Z,Katsumi H,Sakane T,Yamamoto A

    更新日期：2010-06-30 00:00:00

  • Stability of luciferase plasmid entrapped in cationic bilayer vesicles.

    abstract：:Characteristics and physical stability of luciferase plasmid (pLuc) entrapped in cationic bilayer vesicles prepared from various molar ratios of amphiphiles (DPPC, Tween61 or Span60), cholesterol (Chol) and cationic charge lipid (DDAB) were investigated. The cationic liposomes were composed of DPPC/Chol/DDAB in the mo...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.01.001

    authors： Manosroi A,Thathang K,Werner RG,Schubert R,Manosroi J

    更新日期：2008-05-22 00:00:00

  • Controlled release injectable liposomal gel of ibuprofen for epidural analgesia.

    abstract：:The epidural administration is used commonly in the treatment of pain. Nonsteroidal anti-inflammatory drugs, especially ibuprofen, would have potential in epidural use. Like many epidurally useful drugs it, however, has a short duration of action, which is a limiting factor. To improve epidural pain treatment, a long-...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(00)00376-8

    authors： Paavola A,Kilpeläinen I,Yliruusi J,Rosenberg P

    更新日期：2000-04-10 00:00:00

  • BCNU-loaded poly(D, L-lactide-co-glycolide) wafer and antitumor activity against XF-498 human CNS tumor cells in vitro.

    abstract：:Implantable polymeric device that can release chemotherapeutic agent directly into central nervous system (CNS) has had an impact on malignant glioma therapy. The purpose of our study was to develop an implantable polymeric device, which can release intact 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) for long-term peri...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(02)00543-4

    authors： Seong H,An TK,Khang G,Choi SU,Lee CO,Lee HB

    更新日期：2003-01-30 00:00:00

  • Tablet capping predictions of model materials using multivariate approach.

    abstract：:The present study demonstrated the prediction of predominant root causes of capping behavior as a function of the powder rheological and the mechanical behavior of Acetaminophen (APAP) and Ibuprofen (IBU). The authors analyzed powder rheological properties for powder blend permeability, pressure drop, and cohesion. Th...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118548

    authors： Basim P,Haware RV,Dave RH

    更新日期：2019-10-05 00:00:00

  • Development of machine learning models of β-cyclodextrin and sulfobutylether-β-cyclodextrin complexation free energies.

    abstract：:A new set of 142 experimentally determined complexation constants between sulfobutylether-β-cyclodextrin and diverse organic guest molecules, and 78 observations reported in literature, were used for the development of the QSPR models by the two machine learning regression methods - Cubist and Random Forest. Similar m...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.03.065

    authors： Merzlikine A,Abramov YA,Kowsz SJ,Thomas VH,Mano T

    更新日期：2011-10-14 00:00:00

  • An approach for chemical stability during melt extrusion of a drug substance with a high melting point.

    abstract：:Poorly water-soluble drug substances that exhibit high melting points are difficult to process by melt extrusion due to chemical instability at high temperatures required for processing. The purpose of this study was to extrude meloxicam (melting point 255°C) by optimizing processing parameters and formulation composi...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.03.070

    authors： Haser A,Huang S,Listro T,White D,Zhang F

    更新日期：2017-05-30 00:00:00

  • Design and evaluation of an aerosol infection chamber for small animals.

    abstract：:In this report, we describe the design of an aerosol exposure chamber to reproducibly produce uniformly distributed clouds of droplet nuclei. The device can deliver desired number of bacilli (20-2000) in lungs of mice. All safety measures to handle infectious bacteria have been incorporated in the design and it is con...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(03)00023-1

    authors： Bhaskar S,Upadhyay P

    更新日期：2003-04-14 00:00:00

  • Pluronic gels for nasal delivery of Vitamin B12. Part I: preformulation study.

    abstract：:Thermoreversible nasal gels of Vitamin B(12) using pluronic PF 127 were aimed to improve absorption and patient compliance. In the present research work, effects of Vitamin B(12) and gel additives, viz. PF concentration, osmolarity, polyethylene glycol (PEG 15000) on thermodynamic properties of phase transitions at ge...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2003.10.005

    authors： Pisal SS,Paradkar AR,Mahadik KR,Kadam SS

    更新日期：2004-02-11 00:00:00

  • Fabrication and in vivo evaluation of ligand appended paclitaxel and artemether loaded lipid nanoparticulate systems for the treatment of NSCLC: A nanoparticle assisted combination oncotherapy.

    abstract：:The aim of present study was to develop folate appended PEGylated solid lipid nanoparticles(SLNs) of paclitaxel(FPS) and artemether(FAS). The SLNs were prepared by employing high pressure homogenization technique. The results of MTT assays revealed better cytotoxicity of FPS when given in combination with FAS on human...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119386

    authors： Khatri H,Chokshi N,Rawal S,Patel BM,Badanthadka M,Patel MM

    更新日期：2020-06-15 00:00:00

  • Comparison of two hard keratinous substrates submitted to the action of a keratinase using an experimental design.

    abstract：:The influence of temperature, pH, keratinase concentration, substrate concentration and incubation time on the soluble proteins released by a new keratinase from Doratomyces microsporus was studied with a second-order experimental design. Only 15 or 18 spectrophotometric analyses were required to determine the optimal...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(01)00749-9

    authors： Vignardet C,Guillaume YC,Michel L,Friedrich J,Millet J

    更新日期：2001-08-14 00:00:00

  • Updates on thermosensitive hydrogel for nasal, ocular and cutaneous delivery.

    abstract：:Thermosensitive hydrogels are in situ gelling systems composed of hydrophilic homopolymers or block copolymers which remain as solutions at room temperature and form gels after administration into the body. Its application in advanced drug delivery has gained significant attention in recent years. The tunable characte...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.01.030

    authors： Wang Q,Zuo Z,Cheung CKC,Leung SSY

    更新日期：2019-03-25 00:00:00

  • Characterization of strain rate sensitivity in pharmaceutical materials using indentation creep analysis.

    abstract：:Understanding how a material's response to stress changes as the stress is applied at different rates is important in predicting performance of pharmaceutical powders during tablet compression. Widely used methods for determining strain rate sensitivity (SRS) are empirically based and can often provide inconsistent or...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.09.006

    authors： Katz JM,Buckner IS

    更新日期：2013-02-14 00:00:00

  • Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: In vitro study with finite and infinite dosage application.

    abstract：:The aim of the present research is to evaluate the influence of different lipid vesicular systems as well as the effect of application mode on skin penetration and deposition behaviors of carboxyfluorescein (hydrophilic model drug) and temoporfin (lipophilic model drug). All of the lipid vesicular systems, including c...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.02.006

    authors： Chen M,Liu X,Fahr A

    更新日期：2011-04-15 00:00:00

  • Validation of fluid bed granulation utilizing artificial neural network.

    abstract：:Three innovative components (an annular gap spray system, a booster bottom and an outlet filter) have been developed by Innojet Technologies to improve fluid bed technology and to reduce the common interference factors (clogging of nozzles and outlet filters, spray loss, spray drying and fluidized bed heterogeneity). ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2004.07.051

    authors： Behzadi SS,Klocker J,Hüttlin H,Wolschann P,Viernstein H

    更新日期：2005-03-03 00:00:00

  • Ciprofloxacin surf-plexes in sub-micron emulsions: a novel approach to improve payload efficiency and antimicrobial efficacy.

    abstract：:The aim of this study was to investigate antimicrobial efficacy and pharmacokinetic profile of ciprofloxacin (CFn) loaded oil-in-water (o/w) submicron emulsion (SE-CFn). This study emphasized on development of hydrophobic ion-pair complexes of CFn with sodium deoxycholate (SDC) [CFn-SDC], which was incorporated in the...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.02.020

    authors： Jain V,Singodia D,Gupta GK,Garg D,Keshava GB,Shukla R,Shukla PK,Mishra PR

    更新日期：2011-05-16 00:00:00

  • DNA nanostructure-based drug delivery nanosystems in cancer therapy.

    abstract：:DNA as a novel biomaterial can be used to fabricate different kinds of DNA nanostructures based on its principle of GC/AT complementary base pairing. Studies have shown that DNA nanostructure is a nice drug carrier to overcome big obstacles existing in cancer therapy such as systemic toxicity and unsatisfied drug effi...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijpharm.2017.09.032

    authors： Wu D,Wang L,Li W,Xu X,Jiang W

    更新日期：2017-11-25 00:00:00