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Comparison of two hard keratinous substrates submitted to the action of a keratinase using an experimental design.

Abstract：

:The influence of temperature, pH, keratinase concentration, substrate concentration and incubation time on the soluble proteins released by a new keratinase from Doratomyces microsporus was studied with a second-order experimental design. Only 15 or 18 spectrophotometric analyses were required to determine the optimal experimental conditions for this keratinase on nail and hoof. This study was carried out by measuring, according to Smith's method, the concentration of soluble proteins released by the enzyme on two substrates: nails and sheep hooves. Results give optimum conditions for the keratinase to release the soluble proteins: pH 8.2, keratinase concentration 0.14% (weight of keratinase lyophilisate/final volume) and substrate concentration 5% (weight of nail powder/final volume) for nails; temperature 38.8 degrees C, pH 9, substrate concentration 5% (weight of hoof powder/final volume) and a 5 h 55 min incubation time for hooves.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Vignardet C,Guillaume YC,Michel L,Friedrich J,Millet J

doi

10.1016/s0378-5173(01)00749-9

keywords:

subject

Has Abstract

pub_date

2001-08-14 00:00:00

pages

115-22

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378517301007499

journal_volume

224

pub_type

杂志文章

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