当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF PHARMACEUTICS期刊下所有文献 > Stability of luciferase plasmid entrapped in cationic bilayer vesicles.

Stability of luciferase plasmid entrapped in cationic bilayer vesicles.

Abstract：

:Characteristics and physical stability of luciferase plasmid (pLuc) entrapped in cationic bilayer vesicles prepared from various molar ratios of amphiphiles (DPPC, Tween61 or Span60), cholesterol (Chol) and cationic charge lipid (DDAB) were investigated. The cationic liposomes were composed of DPPC/Chol/DDAB in the molar ratio of 7:2:1. The cationic (Tween61 or Span60) niosomes were composed of Tween61/Chol/DDAB or Span60/Chol/DDAB in the molar ratio of 1:1:0.05. The maximum loading of pLuc was 15.29, 22.70, and 18.92 microg/mg of the total lipids or surfactants of liposomes, Tween61 and Span60 niosomes, respectively. The morphology of the vesicles showing multilamellar structure was characterized by transmission electron microscope (TEM). The particle sizes of the vesicles in nanosize range (160-850 nm) were determined by Photon Correlation Spectroscopy (PCS). Gel electrophoresis and gel documentation were modified to determine the entrapment efficiency of pLuc in cationic bilayer vesicles. The cationic bilayer vesicles gave the pLuc entrapment efficiency of 100%. The pLuc entrapped in cationic liposomes exhibited higher stability than pLuc in solution and pLuc entrapped in cationic Tween61 or Span60 niosomes, when stored at 4, 30 and 50 degrees C for 8 weeks. After 8 weeks at 4 degrees C, pLuc contents remained in cationic liposomes was 2 and 3 times higher than cationic Span60 and Tween61 niosomes, respectively. After 3 weeks, 50 and 2% of pLuc was remained in cationic liposomes at 30 and 50 degrees C respectively, whereas all pLuc in cationic Span 60 and Tween61 niosomes were degraded within 2 and 1 week, respectively. At 30 and 50 degrees C, pLuc in an aqueous solution or in bilayer vesicular formulations were not in a stable supercoil form. This study has indicated that the stability of pLuc can be enhanced by entrapping in cationic liposomes more than in niosomes. Higher temperature with increase storage time can affect the stability of pLuc even entrapped in bilayer vesicles.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Manosroi A,Thathang K,Werner RG,Schubert R,Manosroi J

doi

10.1016/j.ijpharm.2008.01.001

subject

Has Abstract

pub_date

2008-05-22 00:00:00

pages

291-9

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378-5173(08)00010-0

journal_volume

356

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF PHARMACEUTICS文献大全
  • Liposome surface charge influence on skin penetration behaviour.

    abstract：:Vesicular systems have shown their ability to increase dermal and transdermal drug delivery. Their mechanism of drug transport into and through the skin has been investigated but remains a much debated question. Several researchers have outlined that drug penetration can be influenced by modifying the surface charge o...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.03.049

    authors： Gillet A,Compère P,Lecomte F,Hubert P,Ducat E,Evrard B,Piel G

    更新日期：2011-06-15 00:00:00

  • Multiscale study on the enhancing effect and mechanism of borneolum on transdermal permeation of drugs with different log P values and molecular sizes.

    abstract：:D-borneolum is commonly used as a permeation enhancer in Traditional Chinese Medicine (TCM) formulas for transdermal application. Additionally, two other sources of borneolums were recorded in the 2015 edition of the Chinese Pharmacopoeia (ChP), including L-borneolum and borneolum syntheticum. To guide the selection a...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119225

    authors： Yang C,Guo S,Wu X,Yang P,Han L,Dai X,Shi X

    更新日期：2020-04-30 00:00:00

  • pH sensitive alginate-guar gum hydrogel for the controlled delivery of protein drugs.

    abstract：:Design of a pH sensitive alginate-guar gum hydrogel crosslinked with glutaraldehyde was done for the controlled delivery of protein drugs. Alginate is a non-toxic polysaccharide with favorable pH sensitive properties for intestinal delivery of protein drugs. Drug leaching during hydrogel preparation and rapid dissolut...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2006.11.009

    authors： George M,Abraham TE

    更新日期：2007-04-20 00:00:00

  • Understanding Flow Properties of Mannitol Powder at a Range of Temperature and Humidity.

    abstract：:Inadequate flowability of powders in industries during handling can cause many problems. For example, lack of flow from hoppers, poor tablet weight consistency, and low production rate in tableting. Many factors are known to commonly affect flow properties of powders, such as temperature, humidity and conditioning dur...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2021.120244

    authors： Salehi H,Karde V,Hajmohammadi H,Dissanayake S,Larsson SH,Y Y Heng J,Bradley M

    更新日期：2021-01-20 00:00:00

  • Peptide ligand-mediated liposome distribution and targeting to EGFR expressing tumor in vivo.

    abstract：:Epidermal growth factor receptor (EGFR) is an important anti-cancer therapy target that is applicable to many cancer types. We had previously reported the screening and discovery of a novel peptide ligand against EGFR named GE11. It was shown to bind to EGFR competitively with EGF and mediate gene delivery to cancer c...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.07.012

    authors： Song S,Liu D,Peng J,Sun Y,Li Z,Gu JR,Xu Y

    更新日期：2008-11-03 00:00:00

  • Development and evaluation of penciclovir-loaded solid lipid nanoparticles for topical delivery.

    abstract：:The objective of this investigation was to develop solid lipid nanoparticles (SLNs) of penciclovir and evaluate the potential of SLNs as the carrier of penciclovir for topical delivery. Penciclovir-loaded SLNs were prepared by a double (W/O/W) emulsion technique. The SLNs presented spherical with the mean diameter of ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.01.014

    authors： Lv Q,Yu A,Xi Y,Li H,Song Z,Cui J,Cao F,Zhai G

    更新日期：2009-05-08 00:00:00

  • GSH responsive nanomedicines self-assembled from small molecule prodrug alleviate the toxicity of cardiac glycosides as potent cancer drugs.

    abstract：:Cardiac glycosides (CGs) have been used to treat cancer for hundreds of years. However, the narrow therapeutic window and system toxicity have hindered their wide clinical applications. Herein, the small molecule prodrug strategy and nanotechnology were integrated into one drug delivery system with enhanced therapeuti...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118980

    authors： Zhang H,Zhu Y,Sun C,Xie Y,Adu-Frimpong M,Deng W,Yu J,Xu X,Han Z,Qi G

    更新日期：2020-02-15 00:00:00

  • Particle engineering of materials for oral inhalation by dry powder inhalers. II-Sodium cromoglicate.

    abstract：:Sodium cromoglicate is an antiasthmatic and antiallergenic drug used in inhalation therapy and commonly administered by a dry powder inhaler. In the present study we sought to examine the feasibility of producing nanoporous microparticles (NPMPs) of this hydrophilic material by adaptation of a spray drying process pre...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.11.040

    authors： Nolan LM,Li J,Tajber L,Corrigan OI,Healy AM

    更新日期：2011-02-28 00:00:00

  • Tableting process optimisation with the application of fuzzy models.

    abstract：:A quality-by-design (QbD) principle, including process analytical technology, is becoming the principal idea in drug development and manufacturing. The implementation of QbD into product development and manufacturing requires larger resources, both human and financial, however, large-scale production can be establishe...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.01.023

    authors： Belic A,Skrjanc I,Bozic DZ,Vrecer F

    更新日期：2010-04-15 00:00:00

  • Maintaining a frozen shipping environment for Phase I clinical trial distribution.

    abstract：:The need for stringent temperature control provides significant challenges to pharmaceutical distributors operating in all sectors of the industry. Products with a frozen storage label requirement can be significantly problematic. This study aimed to provide evidence of robust and reproducible frozen shipment arrangem...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2007.06.008

    authors： Elliott MA,Halbert GW

    更新日期：2008-01-04 00:00:00

  • Formation, characterization and pH dependence of rifampicin: heptakis(2,6-di-O-methyl)-β-cyclodextrin complexes.

    abstract：:Rifampicin (Rif) is a broad spectrum antibiotic used as a first line agent in the treatment of mycobacterial infections. However, its low solubility and reduced stability in water limit its bioavailability, thus requiring the use of complex formulations. Here, we present a systematic study of Rif in complex with a met...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.06.015

    authors： Angiolini L,Agnes M,Cohen B,Yannakopoulou K,Douhal A

    更新日期：2017-10-15 00:00:00

  • A new expanded solubility parameter approach.

    abstract：:The partial or Hansen solubility parameters (HSP) are important properties of the various substances and very useful tools for the selection of their solvents or the prediction of their behaviour in numerous applications. Their design and evaluation relies on the basic rule of "similarity matching" for solubility. The...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.01.001

    authors： Stefanis E,Panayiotou C

    更新日期：2012-04-15 00:00:00

  • Optimization of PLG microspheres for tailored drug release.

    abstract：:Here we explore the opportunity to design and then produce tailored release of therapeutic drugs from microcapsules. By use of "building blocks," formed from well characterized microcapsule populations, an inverse design algorithm has been developed that provides an optimal (in a least squares sense) combination of bu...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.09.010

    authors： Berchane NS,Jebrail FF,Andrews MJ

    更新日期：2010-01-04 00:00:00

  • A system for the production and delivery of monodisperse salbutamol aerosols to the lungs.

    abstract：:An aerosol system is described for the generation and delivery of measured doses of monodisperse therapeutic drug particles to the human lungs. The system comprises a spinning top aerosol generator (STAG), aerosol chamber and inhalation control unit. Monodisperse aerosols allow drug particle size effects to be studied...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(03)00032-2

    authors： Biddiscombe MF,Usmani OS,Barnes PJ

    更新日期：2003-03-26 00:00:00

  • New studies on leachables in commercial scale protein drug filling lines using stir bar sorptive extraction coupled with TD-GC-MS and UPLC/QTOF-MS/MS analytics.

    abstract：:The increasing application of Single-Use Systems (SUSs) in pharmaceutical manufacturing lines poses a potential risk of polymer-related impurities leaching into the process stream and persisting through the manufacturing process. To minimize any potential toxicity and impairment to the product's quality, safety thresh...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.11.033

    authors： Scherer N,Marcseková K,Posset T,Winter G

    更新日期：2019-01-30 00:00:00

  • Approaches to determine the enthalpy of crystallisation, and amorphous content, of lactose from isothermal calorimetric data.

    abstract：:Amorphous lactose will crystallise rapidly if its glass transition temperature is reduced below its storage temperature. This is readily achieved by storing samples at ambient temperature and a relative humidity (RH) of greater than 50%. If the sample is monitored in an isothermal microcalorimeter as it crystallises, ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2004.07.016

    authors： Dilworth SE,Buckton G,Gaisford S,Ramos R

    更新日期：2004-10-13 00:00:00

  • Retention and distribution of two 99mTc-DTPA labelled vaginal dosage forms.

    abstract：UNLABELLED:To objectively evaluate the performance of new vaginal dosage forms, it is important to determine their time of residence and their distribution. This paper describes the in vivo characteristics of a reference and test product in this situation. METHOD:A randomised cross-over study was performed in the same...

    journal_title：International journal of pharmaceutics

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.ijpharm.2003.11.006

    authors： Chatterton BE,Penglis S,Kovacs JC,Presnell B,Hunt B

    更新日期：2004-03-01 00:00:00

  • Lectin-coated PLGA microparticles: thermoresponsive release and in vitro evidence for enhanced cell interaction.

    abstract：:PLGA-microparticles with 4.7 μm in diameter were prepared by the double emulsion technique and loaded with 1.7 μg fluorescein/mg PLGA mimicking a hydrophilic API. In an effort to further elucidate the release and bioadhesive characteristics of lectin-grafted formulations in vitro, the particles were coated with wheat ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.07.025

    authors： Wang XY,Koller R,Wirth M,Gabor F

    更新日期：2012-10-15 00:00:00

  • A miniaturized in vitro release method for investigating drug-release mechanisms.

    abstract：:We have evaluated a miniaturized in vitro method, based on the μDISS Profiler™ technique that enables on-line monitoring of drug release from a 21 μl sample with 10 ml of release medium. Four model drugs in eight clinically used formulations, including both solid and non-solid drug delivery systems, were investigated....

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.03.076

    authors： Ahnfelt E,Sjögren E,Axén N,Lennernäs H

    更新日期：2015-01-01 00:00:00

  • Danshen extract does not alter pharmacokinetics of docetaxel and clopidogrel, reflecting its negligible potential in P-glycoprotein- and cytochrome P4503A-mediated herb-drug interactions.

    abstract：:Danshen (Salvia miltiorrhiza) contains tanshinones, which inhibit P-glycoprotein (P-gp) and the cytochrome P450 (CYP) system. In the present study, we evaluated the possible pharmacokinetic interactions of Danshen extract with docetaxel and clopidogrel in rats. Docetaxel (5 mg/kg intravenously and 40 mg/kg orally) or ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.03.031

    authors： Lee JH,Shin YJ,Kim HJ,Oh JH,Jang YP,Lee YJ

    更新日期：2011-05-30 00:00:00

  • Aerosolized semifluorinated alkanes as excipients are suitable for inhalative drug delivery--a pilot study.

    abstract：:Semifluorinated alkanes (SFAs) have been described as potential excipients for pulmonary drug delivery, but proof of their efficacy is still lacking. We tested whether SFA formulations with the test drug ibuprofen can be nebulised and evaluated their pharmacokinetics. Physico-chemical properties of five different ibup...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.10.051

    authors： Tsagogiorgas C,Jung T,Krebs J,Theisinger B,Beck G,Yard BA,Quintel M

    更新日期：2012-01-17 00:00:00

  • Comparative scintigraphic assessment of the intragastric distribution and residence of cholestyramine, Carbopol 934P and sucralfate.

    abstract：:It has been demonstrated that orally administered cholestyramine is distributed throughout the stomach and provides prolonged gastric residence via mucoadhesion. Gamma scintigraphy was used to compare the gastric emptying and residence of this resin with two formulations known to exhibit retentive or bioadhesive prope...

    journal_title：International journal of pharmaceutics

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0378-5173(00)00600-1

    authors： Jackson SJ,Bush D,Perkins AC

    更新日期：2001-01-05 00:00:00

  • Distribution of nobiletin chitosan-based microemulsions in brain following i.v. injection in mice.

    abstract：:The purpose of this study was to characterize the in vitro properties of a number of chitosan-based microemulsions containing nobiletin and determine its distribution in mice brain following i.v. administration. The phase behavior and properties of chitosan-based microemulsions were investigated in a pseudo-ternary sy...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2007.10.010

    authors： Yao J,Zhou JP,Ping QN,Lu Y,Chen L

    更新日期：2008-03-20 00:00:00

  • Liposome-like nanocapsules of dual drug-tailed betaine for cancer therapy.

    abstract：:A novel dual drug-tailed betaine conjugate amphiphile has been firstly synthesized in which the polar headgroup is derived from glycine betaine and the hydrophobic tails are chlorambucil molecules. The newly prepared conjugate undergoes self-assembly to form stable liposome-like nanocapsules as an effective carrier wi...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.08.006

    authors： Fang S,Niu Y,Zhang W,Zhang Y,Yu L,Zhang Y,Li X

    更新日期：2015-09-30 00:00:00

  • PAMAM dendrimers and model membranes: differential scanning calorimetry studies.

    abstract：:Dendrimers attract much attention as potential drug and gene carriers for intracellular delivery. From this point of view, it is crucial to extend our knowledge about their interactions with membranes. The influence of polyamidoamine (PAMAM) dendrimers on the thermotropic behavior of DPPC multilamellar vesicles and DM...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2005.08.015

    authors： Klajnert B,Epand RM

    更新日期：2005-11-23 00:00:00

  • A Design of Experiment (DoE) approach to optimise spray drying process conditions for the production of trehalose/leucine formulations with application in pulmonary delivery.

    abstract：:The present study evaluates the effect of L-leucine concentration and operating parameters of a laboratory spray dryer on characteristics of trehalose dry powders, with the goal of optimizing production of these powders for inhaled drug delivery. Trehalose/L-leucine mixtures were spray dried from aqueous solution usin...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.03.004

    authors： Focaroli S,Mah PT,Hastedt JE,Gitlin I,Oscarson S,Fahy JV,Healy AM

    更新日期：2019-05-01 00:00:00

  • Novel O-palmitoylscleroglucan-coated liposomes as drug carriers: development, characterization and interaction with leuprolide.

    abstract：:Polysaccharide-coated liposomes have been studied for their potential use for peptide drug delivery by the oral route because they are able to minimize the disruptive influences on peptide drugs of gastrointestinal fluids. The aim of this work was to synthesize and characterize a modified polysaccharide, O-palmitoylsc...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2006.06.040

    authors： Carafa M,Marianecci C,Annibaldi V,Di Stefano A,Sozio P,Santucci E

    更新日期：2006-11-15 00:00:00

  • Dependency of cyclosporine tissue distribution and metabolism on the age and gender of rats after a single intravenous dose.

    abstract：:In a previous study we demonstrated the dependency of cyclosporine (CyA) pharmacokinetics on the age and gender of Wistar rats given 10 mg/kg intravenously. The present study has been conducted under the same experimental conditions (10 mg/kg as a single intravenous dose) to identify the mechanisms behind such differe...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00461-5

    authors： Molpeceres J,Chacón M,Guzmán M,Aberturas MR,Berges L

    更新日期：2000-03-20 00:00:00

  • The influence of formulation variables on in vitro transfection efficiency and physicochemical properties of chitosan-based polyplexes.

    abstract：:The aim of this study was to investigate how a selection of formulation variables affects the in vitro transfection efficiency and physicochemical properties (particle size, zetapotential and chitosan-plasmid association) of chitosan-based polyplexes. Experimental designs in combination with multivariate data analysis...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(03)00301-6

    authors： Romøren K,Pedersen S,Smistad G,Evensen Ø,Thu BJ

    更新日期：2003-08-11 00:00:00

  • Lectin-mediated drug delivery: binding and uptake of BSA-WGA conjugates using the Caco-2 model.

    abstract：:To examine whether the dietary lectin wheat germ agglutinin (WGA) can facilitate binding and uptake of protein drugs due to its cytoadhesive and cytoinvasive properties, conjugates were prepared by covalent coupling of fluorescein-labeled bovine serum albumin (F-BSA) to WGA using divinylsulfone for crosslinking. Incre...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(02)00049-2

    authors： Gabor F,Schwarzbauer A,Wirth M

    更新日期：2002-04-26 00:00:00