Abstract：
:The aim of this study was to investigate how a selection of formulation variables affects the in vitro transfection efficiency and physicochemical properties (particle size, zetapotential and chitosan-plasmid association) of chitosan-based polyplexes. Experimental designs in combination with multivariate data analysis were applied to reveal the effects of the formulation variables on the responses. The following formulation variables were studied: molecular weight and degree of acetylation of chitosan, pH and ionic strength of the buffer in which chitosan was dissolved, charge ratio of polyplexes, plasmid concentration and inclusion of a coacervation agent in the plasmid solution. The in vitro transfection efficiency in Epithelioma papulosum cyprini (EPC) cells was affected by the polyplex charge ratio, the DNA concentration in the complexes as well as the molecular weight and degree of acetylation of the chitosans. Two favourable formulations were identified in a more thorough investigation. These formulations were made of SC113 (theoretical charge ratio 10) and SC214 (theoretical charge ratio 3). The size of the complexes was affected by the degree of acetylation, concentration of DNA, pH, inclusion of a coacervation agent and the charge ratio. The charge ratio, pH and ionic strength determined the zetapotential of the particles, while the charge ratio was important for the association between the plasmid and chitosan.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Romøren K,Pedersen S,Smistad G,Evensen Ø,Thu BJdoi
10.1016/s0378-5173(03)00301-6keywords:
subject
Has Abstractpub_date
2003-08-11 00:00:00pages
115-27issue
1-2eissn
0378-5173issn
1873-3476pii
S0378517303003016journal_volume
261pub_type
杂志文章abstract：:In vivo drug release from solid lipid nanoparticles (SLN) takes place by diffusion and degradation of the lipid matrix. SLN with different degree of crystallinity were prepared to study the effect of crystallinity on the degradation velocity. These SLN were produced by using glycerides with different length of fatty a...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/s0378-5173(02)00035-2
更新日期：2002-04-26 00:00:00
abstract：:A physiologically pertinent in vitro model is urgently needed for probing interactions between inhaled drugs and the organic cation transporters (OCT) in the bronchial epithelium. This study evaluated OCT expression, functionality, inhibition by common inhaled drugs and impact on formoterol transepithelial transport i...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2009-02-23 00:00:00
abstract：:One of the new strategies to improve cancer chemotherapy is based on new drug delivery systems, like the polyethylene glycol-coated superparamagnetic iron oxide nanoparticles (PEG-SPION, thereafter called PS). In this study, PS are loaded with doxorubicin (DOX) anticancer drug, using a pre-formed DOX-Fe(2+) complex re...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2011.06.010
更新日期：2012-02-14 00:00:00
abstract：:The aim of this study was to generate a new type of nanoparticles made of chitosan (CS) and carboxymethyl-beta-cyclodextrin (CM-beta-CD) and to evaluate their potential for the association and delivery of macromolecular drugs. CS and CM-beta-CD or mixtures of CM-beta-CD/tripolyphosphate (TPP) were processed to nanopar...
journal_title：International journal of pharmaceutics
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更新日期：2007-08-01 00:00:00
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/s0378-5173(02)00588-4
更新日期：2003-01-30 00:00:00
abstract：:Dextran nanobubbles were prepared with a dextran shell and a perfluoropentan core in which oxygen was stored. To increase the stability polyvinylpirrolidone was also added to the formulation as stabilizing agent. Rhodamine B was used as fluorescent marker to obtain fluorescent nanobubbles. The nanobubble formulations ...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2009.07.010
更新日期：2009-11-03 00:00:00
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journal_title：International journal of pharmaceutics
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更新日期：2013-11-01 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/s0378-5173(03)00234-5
更新日期：2003-07-09 00:00:00
abstract：:Polyamidoamine-polylysine graft copolymers (PAMAM-g-PLL) were prepared by ring-opening polymerization of benzyloxycarbonyl lysine N-carboxyanhydride (Lys(Z)-NCA) initiated with primary amine of generation 4 polyamidoamine (PAMAM G4) and subsequent deprotection of polyamidoamine-poly-(benzyloxycarbonyl lysine) copolyme...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2011.08.036
更新日期：2011-11-28 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
doi：10.1016/j.ijpharm.2018.10.011
更新日期：2018-12-01 00:00:00
abstract：:The aim of the current study is to explore the potential of PEG-30-dipolyhydroxystearate (Cithrol(®) DPHS) and Soluplus(®) as ingredients in novel semisolid self-nanoemulsifying drug delivery systems (SNEDDS). Semisolid SNEDDS consisting of Cithrol(®) DPHS, Capmul(®) MCM and Kolliphor(®) HS 15 were successfully prepar...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2014.10.063
更新日期：2014-12-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2014.04.027
更新日期：2014-07-01 00:00:00
abstract：:The objective of the present study was to synthesize and investigate the transgene expression efficacy of quaternized derivatives of aminoglycoside polymers in different cancer cell lines. A series of glycidyltrimethylammonium chloride (GTMAC) derivatives of aminoglycoside polymers (GTMAC-AM polymers), containing vary...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2015.04.026
更新日期：2015-07-15 00:00:00
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abstract：:The purpose of present study was to assess the enhancing effect of resveratrol (Res) on the absorption of bestatin and clarify the related molecular mechanism. Res facilitated bestatin absorption by down-regulating both protein and gene levels of multidrug resistance 1 (Mdr1) and Multidrug resistance-associated protei...
journal_title：International journal of pharmaceutics
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abstract：:A novel thiomer derivative of glycol chitosan (GCS) was synthesized by coupling with thioglycolic acid (TGA) and evaluated for the pulmonary delivery of peptides. Nanoparticles (NPs) based on GCS and GCS-TGA were obtained by the ionic gelation method and demonstrated a particle size in the range of 0.23-0.33 microm wi...
journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
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更新日期：2018-09-05 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2006.06.044
更新日期：2006-10-31 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2007.07.004
更新日期：2008-02-04 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2004.07.041
更新日期：2005-03-03 00:00:00
abstract：:The objective of this study was the development of different solid formulations, such as wafers and films, for buccal administration of ondansetron, a selective and potent antagonist of 5-hydroxytryptamine 3 receptors used in children for the treatment of nausea and vomiting. Wafers and films have been prepared drying...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2020-04-30 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2014-10-20 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/s0378-5173(02)00539-2
更新日期：2003-01-02 00:00:00
abstract：:Recently, we synthesized novel amphiphilic gelatin-oleic acid (GO) conjugate to prepare self-assembled nanoparticles for drug delivery. The aim of this study was to investigate pharmaceutical potentialities of self-assembled GO nanoparticles for solubility enhancement and modified release of poorly water-soluble drugs...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2013.07.025
更新日期：2013-10-15 00:00:00
abstract：:In this paper, a fast forming nano-composite hydrogel was developed for potential application in ocular drug delivery. The optical transmission (OT) as well as rheological properties of nano-composite hydrogel was characterized. The developed nano-composite hydrogel given a high diclofenac micelles loading and provide...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
doi：10.1016/j.ijpharm.2013.03.024
更新日期：2013-05-01 00:00:00
abstract：:The pharmacokinetics of acetaminophen have been well studied in different populations, especially in Caucasians. However, limited studies on acetaminophen pharmacokinetics have been conducted in the native Chinese and few such data have been reported in the English language literature. Previous published studies sugge...
journal_title：International journal of pharmaceutics
pub_type： 临床试验,杂志文章
doi：10.1016/s0378-5173(01)00712-8
更新日期：2001-07-17 00:00:00
