Abstract:
OBJECTIVES:Recently, novel biphasic calcium phosphate (BCP) scaffolds have emerged as a new class of bone grafts with osteoinductive potential demonstrating the ability to form ectopic bone in extra-skeletal sites. The aim of the present study was to perform an osteogenic gene array to target possible genes responsible for eliciting the changes in cell expression responsible for inducing osteoblast differentiation. MATERIALS AND METHODS:Human MG63 osteoblast-like cells were seeded for 24 h on tissue culture plastic or osteoinductive BCP particles and analyzed for upregulated genes using an osteogenesis super-array. Osteoblast-related genes including those transcribed during bone mineralization, bone metabolism, cell growth and differentiation, as well as gene products representing extracellular matrix molecules, transcription factors, and cell adhesion molecules were investigated. RESULTS:An upregulation of genes transcribing biglycan (1.7-fold), bone morphogenetic proteins 1, 2, 4, 6, and 7 (1.5-2.1-fold), various collagen isoforms including 1a1, 1a2, 2a1, and 5a1 (1.73-2.72-fold), colony stimulating factor 2 (2.59-fold), fibroblast growth factor receptor 2 (1.79-fold), fibronectin (2.56-fold), integrin alpha 1, 2, and 3 (1.82-2.24-fold), SOX9 (2.75-fold), transforming growth factor beta receptor 2 (1.72-fold), vitamin D (1.89-fold), and vascular endothelial growth factor A and B (2.00, 1.75-fold) were all significantly (p < 0.05) increased on BCP particles when compared to control tissue culture plastic. CONCLUSION:In summary, a number of activated genes were involved in bone formation following osteoblast attachment to BCP particles. The involvement of key chondrogenic genes hints that bone grafts capable of spontaneously inducing ectopic bone formation may implicate endochondral ossification. Further investigations in the triggered pathways involved in the process of ectopic bone formation are necessary to understand the key inductive properties of these novel osteoinductive BCP particles. CLINICAL RELEVANCE:Novel osteoinductive BCP particles demonstrate a wide range of significant increases over several key molecules implicated in osteogenesis that may be implicated in their ability to form ectopic bone formation.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Miron RJ,Shuang Y,Bosshardt DD,Caballé-Serrano J,Chandad F,Zhang Ydoi
10.1007/s00784-016-1825-0subject
Has Abstractpub_date
2017-04-01 00:00:00pages
801-808issue
3eissn
1432-6981issn
1436-3771pii
10.1007/s00784-016-1825-0journal_volume
21pub_type
杂志文章abstract:OBJECTIVES:The aim of the present study was to determine the prevalence of permanent first molar (P1M) extraction among Turkish adolescents and young adult subpopulation, and to investigate the effects of P1M extraction on development of the third molars (3Ms) in the same quadrant. MATERIALS AND METHODS:A retrospectiv...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1121-1
更新日期:2014-01-01 00:00:00
abstract::Resin-bonded fixed partial dentures (RBFPD) are used as a minimal invasive, tooth-preventing alternative for replacing anterior teeth. Zirconia cantilever restorations were supposed to show sufficient strength for a clinical application. The aim of this investigation was to determine the fracture characteristics of ca...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0254-8
更新日期:2009-12-01 00:00:00
abstract::To present the safety profile, the early healing phase and the clinical outcomes at 24 weeks following treatment of human intrabony defects with open flap debridement (OFD) alone or with OFD and rhGDF-5 adsorbed onto a particulate β-tricalcium phosphate (β-TCP) carrier. Twenty chronic periodontitis patients, each with...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-011-0610-3
更新日期:2012-08-01 00:00:00
abstract::The goal of the study was to develop a validated manual preparation process that conforms to the requirements of validation guidelines. Twelve dental transmission devices from various manufacturers (turbines, handpieces, and contra-angle handpieces) were artificially contaminated with bovine hemoglobin for the test. T...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-010-0425-7
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to investigate whether the genetic variations which could regulate inflammatory responses were associated with the risk of peri-implantitis. MATERIALS AND METHODS:We evaluated three genetic variants including tumor necrosis factor-alpha (TNF-α) - 308G/A, interleukin-1 alpha (IL...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02968-z
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:Lithium disilicate can easily be machined by CAM techniques in its metasilicate status. Due to slightly inferior material properties, the material is not yet recommended for three-unit fixed dental prosthesis (FDP) as it is the case for the press material up to the second bicuspid. Therefore, the aim of this...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1191-8
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVE:Aggregatibacter actinomycetemcomitans, specially its highly leucotoxic strain (JP2 clone), represents an etiological factor for the onset and progression of aggressive types of periodontitis. The aims of this investigation were to investigate the most relevant periodontal pathogens in the subgingival microbio...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1653-7
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to examine the influence of experience and gender on the radiographic detection of proximal caries lesions and on therapeutic decisions. MATERIALS AND METHODS:A survey of 220 examiners (students and employees at three universities and dentists in the field) was taken using a standa...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2078-2
更新日期:2017-12-01 00:00:00
abstract::The aim of this study was to determine the prevalence of dental erosion in young Icelandic adults (19-22 years old) and patients with gastroesophageal reflux disease (GERD), in relation to their soft drink consumption and gastroesophageal reflux. Eighty subjects (40 males and 40 females), comprising 57 young adults (m...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-003-0252-1
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVES:To assess how to control, detect, and treat secondary caries. This review serves to inform a joint ORCA/EFCD consensus process. METHODS:Systematic and non-systematic reviews were performed or consulted and narratively synthesized. RESULTS:Secondary (or recurrent) caries is defined as a lesion associated wi...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-020-03268-7
更新日期:2020-05-01 00:00:00
abstract::Peoples in Iraq face a mixture of health hazards associated with poverty. Oral cancer is a major public health issue worldwide; it remains a highly lethal and disfiguring disease. It is primarily a disease of epithelial origin. A total of 1,425 cases of histologically diagnosed squamous cell carcinoma collected from t...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-007-0141-0
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVE:This study aimed to prospectively observe gustatory and neurosensory alterations following surgical removal of mandibular third molars. MATERIAL AND METHODS:A prospective clinical study was conducted with patients who required mandibular third molar extraction, recruited from the Division of Oral and Maxillo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-02798-5
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:There is a long history of studies into cystic lesions of the jaws. However, there is little data on postoperative bone regeneration. The aim of the study was to calculate the size of the residual bone lesions after cystostomies and cystectomies to predict the postoperative bone healing. MATERIAL AND METHOD...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02826-y
更新日期:2019-11-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2235-7
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:Cell-free DNA (cfDNA) harboring mutations has been found in patients with diseases. Experimental studies have shown that cfDNA can be transmitted, leading to transformations in the host. In the present study, we evaluated whether bone allograft material contains cfDNA and whether this foreign cfDNA can be re...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02867-3
更新日期:2019-12-01 00:00:00
abstract::Airway management in infants with Pierre Robin sequence (PRS) is often challenging. Advancement of the tongue base using mandibular distraction in neonates can avoid tracheotomy and is increasingly propagated. The osteotomies can be performed via intra- and extraoral approaches. Nowadays, for precise bone cutting, pie...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-006-0091-y
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the contamination level of toothbrushes by mutans streptococci (MS) and the efficacy of antimicrobial solutions: cetylpyridinium chloride 0.05 % (CPC; Cepacol™) and chlorhexidine 0.12 % (CHX; Periogard™), to disinfect toothbrushes of preschool-aged children in day-care centers. MATERIAL AND METHO...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-013-1169-y
更新日期:2014-11-01 00:00:00
abstract:OBJECTIVES:The aim of this systematic review was to assess the efficacy of bioactive glass (BG) in bone regeneration for implant site development procedures. MATERIAL AND METHODS:The following specific question was formulated with reference to Population, Intervention, Control, Outcomes (PICO): "In persons that underg...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-014-1376-1
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to establish the prevalence of celiac disease (CD) in patients with erosive and reticular form of oral lichen planus (OLP) compared to healthy controls. MATERIALS AND METHODS:Tissue transglutaminase antibodies (anti-tTG) and antibodies against gliadin (AGA) were measured in the ser...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1288-0
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVES:The present study aims to investigate the relevance of immunohistochemical p53 expression in carcinomas of the oral cavity and of the head and neck region. Long-term clinical and histopathological follow-up findings as well as HPV status are correlated with the results of this examination. MATERIALS AND MET...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0939-x
更新日期:2014-01-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1314-2
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to validate a standardized pragmatic approach to manage new oral anticoagulants (NOACs) in patients who undergo dental extractions. MATERIALS AND METHODS:This prospective case-control study in patients undergoing dental extraction included 26 patients (mean age 76 years, 57% male) ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-2010-1
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:This study aimed to investigate the root canal morphology of mandibular first premolar teeth in a population from southwestern China by micro-computed tomography (micro-CT). MATERIALS AND METHODS:Human mandibular first premolars (115) were selected and prepared for micro-CT analysis with a slice thickness o...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0778-1
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:Reconstruction of large and complex bone segments is a challenging problem facing maxillofacial surgery. The majority of current regenerative approaches rely on extrinsic vascularization, which is deficient after cancer ablation and irradiation. The aim of the study was to investigate the efficacy of intrinsi...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1143-8
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to investigate the effects of fixed functional therapy on oropharyngeal airway dimensions and hyoid bone positions in Class II patients and make comparison with an untreated Class II group. MATERIALS AND METHODS:Eighteen patients (8 girls, 10 boys; mean age 13.62 ± 1.92 years)...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1659-1
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to investigate the relationship between periodontal morphometric parameters and to develop a biotype classification based on the variables examined with the use of cone-beam computed tomography (CBCT). MATERIALS AND METHODS:Forty-two periodontally healthy subjects that underwent a C...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1694-y
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVES:To assess and compare the pharmacological effect of different drugs on pain relief after periodontal surgery. MATERIALS AND METHODS:Five databases were searched up to September 2019. The eligible studies comprised randomized clinical trials, involving only adult individuals that received any periodontal sur...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-020-03401-6
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVE:This study aimed to compare (18)F-fluorodesoxyglucose positron emission tomography/MRI ((18)F-FDG-PET-MRI) fusion images, including diffusion-weighted imaging (DWI), (18)F-FDG-PET/CT, and ultrasound (US) regarding their performance in nodal staging of patients with head and neck squamous cell carcinoma (HNSCC...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1050-z
更新日期:2014-04-01 00:00:00
abstract::From a preventive point of view collection of data concerning carious and non-carious cervical tooth defects is definitely important. Consequently, the prevalence and distribution of different root lesions were studied and correlated with behavioral and biological factors in 50- to 60-year-old German individuals (n = ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050097
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate the effect of early functional loading to plateaued implants on bone formation and implant stability in a dog model. MATERIALS AND METHODS:Early loading (EL), nonloading control, and delayed loading (DL) groups were compared using six beagle dogs under functional lo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0929-z
更新日期:2014-01-01 00:00:00