Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states.

abstract：:Cranial neural crest (CNC) cells give rise to bone, cartilage, tendons, and ligaments of the vertebrate craniofacial musculoskeletal complex, as well as regulate mesoderm-derived craniofacial muscle development through cell-cell interactions. Using the mouse soft palate as a model, we performed an unbiased single-cell...

journal_title：eLife

authors： Han X,Feng J,Guo T,Loh YE,Yuan Y,Ho TV,Cho CK,Li J,Jing J,Janeckova E,He J,Pei F,Bi J,Song B,Chai Y

更新日期：2021-01-22 00:00:00

abstract：:Previously, we identified long repeat sequences that are frequently associated with genome rearrangements, including copy number variation (CNV), in many diverse isolates of the human fungal pathogen Candida albicans (Todd et al., 2019). Here, we describe the rapid acquisition of novel, high copy number CNVs during ad...

journal_title：eLife

authors： Todd RT,Selmecki A

更新日期：2020-07-20 00:00:00

abstract：:We have previously demonstrated that checkpoint kinase 2 (CHK2) is a critical negative regulator of androgen receptor (AR) transcriptional activity, prostate cancer (PCa) cell growth, and androgen sensitivity. We have now uncovered that the AR directly interacts with CHK2 and ionizing radiation (IR) increases this int...

journal_title：eLife

authors： Ta HQ,Dworak N,Ivey ML,Roller DG,Gioeli D

更新日期：2020-06-24 00:00:00

abstract：:Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...

journal_title：eLife

authors： Baldi S,Bolognesi A,Meinema AC,Barral Y

更新日期：2017-12-28 00:00:00

abstract：:Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocy...

journal_title：eLife

authors： Manzoni G,Marinach C,Topçu S,Briquet S,Grand M,Tolle M,Gransagne M,Lescar J,Andolina C,Franetich JF,Zeisel MB,Huby T,Rubinstein E,Snounou G,Mazier D,Nosten F,Baumert TF,Silvie O

更新日期：2017-05-16 00:00:00

abstract：:Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1 adipocytes with specific inhibitors of the...

journal_title：eLife

authors： Mowers J,Uhm M,Reilly SM,Simon J,Leto D,Chiang SH,Chang L,Saltiel AR

更新日期：2013-12-24 00:00:00

abstract：:It is widely argued that personalized instruction based on individual differences in learning styles or genetic predispositions could improve learning outcomes. However, this proposition has resisted clear demonstration in human studies, where it is difficult to control experience and quantify outcomes. Here, we take ...

journal_title：eLife

authors： Mets DG,Brainard MS

更新日期：2019-09-17 00:00:00

abstract：:Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system. Current methods do not enable precise engineering of complex genotypes that can be easily tracked in a mixed ...

journal_title：eLife

authors： Bak RO,Dever DP,Reinisch A,Cruz Hernandez D,Majeti R,Porteus MH

更新日期：2017-09-28 00:00:00

abstract：:Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators, and biological effects of terrein production were studied in the fun...

journal_title：eLife

authors： Gressler M,Meyer F,Heine D,Hortschansky P,Hertweck C,Brock M

更新日期：2015-07-14 00:00:00

abstract：:Adhesion molecules hold cells together but also couple cell membranes to a contractile actomyosin network, which limits the expansion of cell contacts. Despite their fundamental role in tissue morphogenesis and tissue homeostasis, how adhesion molecules control cell shapes and cell patterns in tissues remains unclear....

journal_title：eLife

authors： Chan EH,Chavadimane Shivakumar P,Clément R,Laugier E,Lenne PF

更新日期：2017-05-24 00:00:00

abstract：:A polymorphism in the autophagy gene Atg16l1 is associated with susceptibility to inflammatory bowel disease (IBD); however, it remains unclear how autophagy contributes to intestinal immune homeostasis. Here, we demonstrate that autophagy is essential for maintenance of balanced CD4(+) T cell responses in the intesti...

journal_title：eLife

authors： Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

更新日期：2016-02-24 00:00:00

abstract：:Many postdoctoral researchers apply for faculty positions knowing relatively little about the hiring process or what is needed to secure a job offer. To address this lack of knowledge about the hiring process we conducted a survey of applicants for faculty positions: the survey ran between May 2018 and May 2019, and r...

journal_title：eLife

authors： Fernandes JD,Sarabipour S,Smith CT,Niemi NM,Jadavji NM,Kozik AJ,Holehouse AS,Pejaver V,Symmons O,Bisson Filho AW,Haage A

更新日期：2020-06-12 00:00:00

abstract：:Striatal dysfunction plays an important role in dystonia, but the striatal cell types that contribute to abnormal movements are poorly defined. We demonstrate that conditional deletion of the DYT1 dystonia protein torsinA in embryonic progenitors of forebrain cholinergic and GABAergic neurons causes dystonic-like twis...

journal_title：eLife

authors： Pappas SS,Darr K,Holley SM,Cepeda C,Mabrouk OS,Wong JM,LeWitt TM,Paudel R,Houlden H,Kennedy RT,Levine MS,Dauer WT

更新日期：2015-06-08 00:00:00

abstract：:Two gene variants provide different levels of protection against sleeping sickness, but this comes with an increased risk of developing chronic kidney disease. ...

journal_title：eLife

authors： Kruzel-Davila E,Skorecki K

更新日期：2017-07-03 00:00:00

abstract：:The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively ...

journal_title：eLife

authors： He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

更新日期：2015-12-08 00:00:00

abstract：:Lymph nodes (LNs) are a common site of metastasis in solid cancers, and cutaneous melanomas show inherent properties of LN colonization. However, interactions between LN stroma and pioneer metastatic cells during metastatic colonization remain largely uncharacterized. Here we studied mice implanted with GFP-expressing...

journal_title：eLife

authors： Singh R,Choi BK

更新日期：2019-12-24 00:00:00

abstract：:Nerve tissue contains a high density of chemical synapses, about 1 per µm3 in the mammalian cerebral cortex. Thus, even for small blocks of nerve tissue, dense connectomic mapping requires the identification of millions to billions of synapses. While the focus of connectomic data analysis has been on neurite reconstru...

journal_title：eLife

authors： Staffler B,Berning M,Boergens KM,Gour A,Smagt PV,Helmstaedter M

更新日期：2017-07-14 00:00:00

abstract：:Journals are exploring new approaches to peer review in order to reduce bias, increase transparency and respond to author preferences. Funders are also getting involved. ...

journal_title：eLife

authors： Rodgers P

更新日期：2017-09-29 00:00:00

abstract：:Habit formation is a behavioral adaptation that automates routine actions. Habitual behavior correlates with broad reconfigurations of dorsolateral striatal (DLS) circuit properties that increase gain and shift pathway timing. The mechanism(s) for these circuit adaptations are unknown and could be responsible for habi...

journal_title：eLife

authors： O'Hare JK,Li H,Kim N,Gaidis E,Ade K,Beck J,Yin H,Calakos N

更新日期：2017-09-05 00:00:00

abstract：:The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. These neurons differ ...

journal_title：eLife

authors： Nagaeva E,Zubarev I,Bengtsson Gonzales C,Forss M,Nikouei K,de Miguel E,Elsilä L,Linden AM,Hjerling-Leffler J,Augustine GJ,Korpi ER

更新日期：2020-08-04 00:00:00

abstract：:Ageing is a progressive decline of intrinsic physiological functions. We examined the impact of ageing on the ultrastructure and function of mitochondria in mouse and fruit flies (Drosophila melanogaster) by electron cryo-tomography and respirometry. We discovered distinct age-related changes in both model organisms. ...

journal_title：eLife

authors： Brandt T,Mourier A,Tain LS,Partridge L,Larsson NG,Kühlbrandt W

更新日期：2017-07-12 00:00:00

abstract：:TMEM16F is activated by elevated intracellular Ca2+, and functions as a small-conductance ion channel and as a phospholipid scramblase. In contrast to its paralogs, the TMEM16A/B calcium-activated chloride channels, mouse TMEM16F has been reported as a cation-, anion-, or non-selective ion channel, without a definite ...

journal_title：eLife

authors： Ye W,Han TW,He M,Jan YN,Jan LY

更新日期：2019-07-18 00:00:00

abstract：:The ellipsoid body (EB) in the Drosophila brain is a central complex (CX) substructure that harbors circumferentially laminated ring (R) neuron axons and mediates multifaceted sensory integration and motor coordination functions. However, what regulates R axon lamination and how lamination affects R neuron function re...

journal_title：eLife

authors： Xie X,Tabuchi M,Brown MP,Mitchell SP,Wu MN,Kolodkin AL

更新日期：2017-06-20 00:00:00

abstract：:Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...

journal_title：eLife

authors： Budzik JM,Swaney DL,Jimenez-Morales D,Johnson JR,Garelis NE,Repasy T,Roberts AW,Popov LM,Parry TJ,Pratt D,Ideker T,Krogan NJ,Cox JS

更新日期：2020-01-17 00:00:00

abstract：:Uromodulin is the most abundant protein in the urine. It is exclusively produced by renal epithelial cells and it plays key roles in kidney function and disease. Uromodulin mainly exerts its function as an extracellular matrix whose assembly depends on a conserved, specific proteolytic cleavage leading to conformation...

journal_title：eLife

authors： Brunati M,Perucca S,Han L,Cattaneo A,Consolato F,Andolfo A,Schaeffer C,Olinger E,Peng J,Santambrogio S,Perrier R,Li S,Bokhove M,Bachi A,Hummler E,Devuyst O,Wu Q,Jovine L,Rampoldi L

更新日期：2015-12-17 00:00:00

abstract：:Most cellular features have a range of states, but understanding the mechanisms responsible for interspecific divergence is a challenge for evolutionary cell biology. Models are developed for the distribution of mean phenotypes likely to evolve under the joint forces of mutation and genetic drift in the face of consta...

journal_title：eLife

authors： Lynch M

更新日期：2018-06-21 00:00:00

abstract：:How proteins harness mechanical force to control function is a significant biological question. Here we describe a human cell surface receptor that couples ligand binding and force to trigger a chemical event which controls the adhesive properties of the receptor. Our studies of the secreted platelet oxidoreductase, E...

journal_title：eLife

authors： Passam F,Chiu J,Ju L,Pijning A,Jahan Z,Mor-Cohen R,Yeheskel A,Kolšek K,Thärichen L,Aponte-Santamaría C,Gräter F,Hogg PJ

更新日期：2018-06-22 00:00:00

abstract：:Previously, we showed that serum and monocytes from patients with CF exhibit an enhanced NLRP3-inflammasome signature with increased IL-18, IL-1β, caspase-1 activity and ASC speck release (Scambler et al. eLife 2019). Here we show that CFTR modulators down regulate this exaggerated proinflammatory response following L...

journal_title：eLife

authors： Jarosz-Griffiths HH,Scambler T,Wong CH,Lara-Reyna S,Holbrook J,Martinon F,Savic S,Whitaker P,Etherington C,Spoletini G,Clifton I,Mehta A,McDermott MF,Peckham D

更新日期：2020-03-02 00:00:00

abstract：:Researchers have identified two genes-follistatin and activin-as having an important role in the ability of certain flatworms to identify wounds that require the production of new tissue. ...

journal_title：eLife

authors： Sánchez Alvarado A

更新日期：2013-09-10 00:00:00

abstract：:How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Ou...

journal_title：eLife

authors： Mallam AL,Sidote DJ,Lambowitz AM

更新日期：2014-12-12 00:00:00