Mechano-redox control of integrin de-adhesion.

abstract：:Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive. Using published and new datasets, we profile a time course of mature...

journal_title：eLife

authors： Zhou L,Lim MYT,Kaur P,Saj A,Bortolamiol-Becet D,Gopal V,Tolwinski N,Tucker-Kellogg G,Okamura K

更新日期：2018-07-19 00:00:00

abstract：:Using a common analysis pipeline to compare data from three major lineages of complex eukaryotes reveals that transcription seems to evolve at a common rate. ...

journal_title：eLife

authors： Odom DT

更新日期：2016-02-11 00:00:00

abstract：:How Mycobacterium tuberculosis (Mtb) rewires macrophage energy metabolism to facilitate survival is poorly characterized. Here, we used extracellular flux analysis to simultaneously measure the rates of glycolysis and respiration in real time. Mtb infection induced a quiescent energy phenotype in human monocyte-derive...

journal_title：eLife

authors： Cumming BM,Addicott KW,Adamson JH,Steyn AJ

更新日期：2018-11-16 00:00:00

abstract：:Microbial fitness screens are a key technique in functional genomics. We present an all-in-one solution, pyphe, for automating and improving data analysis pipelines associated with large-scale fitness screens, including image acquisition and quantification, data normalisation, and statistical analysis. Pyphe is versat...

journal_title：eLife

authors： Kamrad S,Rodríguez-López M,Cotobal C,Correia-Melo C,Ralser M,Bähler J

更新日期：2020-06-16 00:00:00

abstract：:Minor and major spliceosomes control splicing of distinct intron types and are thought to act largely independent of one another. SR proteins are essential splicing regulators mostly connected to the major spliceosome. Here, we show that Srsf10 expression is controlled through an autoregulated minor intron, tightly co...

journal_title：eLife

authors： Meinke S,Goldammer G,Weber AI,Tarabykin V,Neumann A,Preussner M,Heyd F

更新日期：2020-04-27 00:00:00

abstract：:Armor plate changes in sticklebacks are a classic example of repeated adaptive evolution. Previous studies identified ectodysplasin (EDA) gene as the major locus controlling recurrent plate loss in freshwater fish, though the causative DNA alterations were not known. Here we show that freshwater EDA alleles have cis-a...

journal_title：eLife

authors： O'Brown NM,Summers BR,Jones FC,Brady SD,Kingsley DM

更新日期：2015-01-28 00:00:00

abstract：:Being exposed to cannabinoids in the womb has different consequences for male and female rats. ...

journal_title：eLife

authors： Musardo S,Bellone C

更新日期：2018-09-28 00:00:00

abstract：:Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host inv...

journal_title：eLife

authors： Gronnier J,Crowet JM,Habenstein B,Nasir MN,Bayle V,Hosy E,Platre MP,Gouguet P,Raffaele S,Martinez D,Grelard A,Loquet A,Simon-Plas F,Gerbeau-Pissot P,Der C,Bayer EM,Jaillais Y,Deleu M,Germain V,Lins L,Mongrand S

更新日期：2017-07-31 00:00:00

abstract：:hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Usin...

journal_title：eLife

authors： Krotee P,Rodriguez JA,Sawaya MR,Cascio D,Reyes FE,Shi D,Hattne J,Nannenga BL,Oskarsson ME,Philipp S,Griner S,Jiang L,Glabe CG,Westermark GT,Gonen T,Eisenberg DS

更新日期：2017-01-03 00:00:00

abstract：:Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well u...

journal_title：eLife

authors： Leypunskiy E,Lin J,Yoo H,Lee U,Dinner AR,Rust MJ

更新日期：2017-07-07 00:00:00

abstract：:Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental mechanism of this lattice structure. We found that this structure emerged early during axon...

journal_title：eLife

authors： Zhong G,He J,Zhou R,Lorenzo D,Babcock HP,Bennett V,Zhuang X

更新日期：2014-12-23 00:00:00

abstract：:Tissue elongation and its control by spatiotemporal signals is a major developmental question. Currently, it is thought that Drosophila ovarian follicular epithelium elongation requires the planar polarization of the basal domain cytoskeleton and of the extra-cellular matrix, associated with a dynamic process of rotat...

journal_title：eLife

authors： Alégot H,Pouchin P,Bardot O,Mirouse V

更新日期：2018-02-08 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is believed to confer protection against Plasmodium falciparum malaria, but the precise nature of the protective effecthas proved difficult to define as G6PD deficiency has multiple allelic variants with different effects in males and females, and it has heterogeneou...

journal_title：eLife

authors： Clarke GM,Rockett K,Kivinen K,Hubbart C,Jeffreys AE,Rowlands K,Jallow M,Conway DJ,Bojang KA,Pinder M,Usen S,Sisay-Joof F,Sirugo G,Toure O,Thera MA,Konate S,Sissoko S,Niangaly A,Poudiougou B,Mangano VD,Bougouma EC

更新日期：2017-01-09 00:00:00

abstract：:Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...

journal_title：eLife

authors： Spletter ML,Barz C,Yeroslaviz A,Zhang X,Lemke SB,Bonnard A,Brunner E,Cardone G,Basler K,Habermann BH,Schnorrer F

更新日期：2018-05-30 00:00:00

abstract：:Unfolded protein responses (UPRs) safeguard cellular function during proteotoxic stress and aging. In a previous paper (Lehrbach and Ruvkun, 2016) we showed that the ER-associated SKN-1A/Nrf1 transcription factor activates proteasome subunit expression in response to proteasome dysfunction, but it was not established ...

journal_title：eLife

authors： Lehrbach NJ,Ruvkun G

更新日期：2019-04-11 00:00:00

abstract：:From bacteria to mammals, different phospholipid species are segregated between the inner and outer leaflets of the plasma membrane by ATP-dependent lipid transporters. Disruption of this asymmetry by ATP-independent phospholipid scrambling is important in cellular signaling, but its mechanism remains incompletely und...

journal_title：eLife

authors： Jiang T,Yu K,Hartzell HC,Tajkhorshid E

更新日期：2017-09-16 00:00:00

abstract：:Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haph...

journal_title：eLife

authors： Ireland WT,Beeler SM,Flores-Bautista E,McCarty NS,Röschinger T,Belliveau NM,Sweredoski MJ,Moradian A,Kinney JB,Phillips R

更新日期：2020-09-21 00:00:00

abstract：:Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) exp...

journal_title：eLife

authors： Bao C,Loerch S,Ling C,Korostelev AA,Grigorieff N,Ermolenko DN

更新日期：2020-05-19 00:00:00

abstract：:Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1 adipocytes with specific inhibitors of the...

journal_title：eLife

authors： Mowers J,Uhm M,Reilly SM,Simon J,Leto D,Chiang SH,Chang L,Saltiel AR

更新日期：2013-12-24 00:00:00

abstract：:The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...

journal_title：eLife

authors： Crouse RB,Kim K,Batchelor HM,Girardi EM,Kamaletdinova R,Chan J,Rajebhosale P,Pittenger ST,Role LW,Talmage DA,Jing M,Li Y,Gao XB,Mineur YS,Picciotto MR

更新日期：2020-09-18 00:00:00

abstract：:The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. These neurons differ ...

journal_title：eLife

authors： Nagaeva E,Zubarev I,Bengtsson Gonzales C,Forss M,Nikouei K,de Miguel E,Elsilä L,Linden AM,Hjerling-Leffler J,Augustine GJ,Korpi ER

更新日期：2020-08-04 00:00:00

abstract：:An analysis of worldwide human genetic variation reveals the footprints of ancient changes in genomic mutation processes. ...

journal_title：eLife

authors： Scally A

更新日期：2017-05-17 00:00:00

abstract：:The human insular cortex is a heterogeneous brain structure which plays an integrative role in guiding behavior. The cytoarchitectonic organization of the human insula has been investigated over the last century using postmortem brains but there has been little progress in noninvasive in vivo mapping of its microstruc...

journal_title：eLife

authors： Menon V,Gallardo G,Pinsk MA,Nguyen VD,Li JR,Cai W,Wassermann D

更新日期：2020-06-04 00:00:00

abstract：:Rats exhibit 'empathy' making them a model to understand the neural underpinnings of such behavior. We show data consistent with these findings, but also that behavior and dopamine (DA) release reflects subjective rather than objective evaluation of appetitive and aversive events that occur to another. We recorded DA ...

journal_title：eLife

authors： Lichtenberg NT,Lee B,Kashtelyan V,Chappa BS,Girma HT,Green EA,Kantor S,Lagowala DA,Myers MA,Potemri D,Pecukonis MG,Tesfay RT,Walters MS,Zhao AC,Blair RJR,Cheer JF,Roesch MR

更新日期：2018-11-28 00:00:00

abstract：:Understanding how genes and experience work in concert to generate phenotypic variability will provide a better understanding of individuality. Here, we considered this in the main olfactory epithelium, a chemosensory structure with over a thousand distinct cell types in mice. We identified a subpopulation of olfactor...

journal_title：eLife

authors： Vihani A,Hu XS,Gundala S,Koyama S,Block E,Matsunami H

更新日期：2020-11-24 00:00:00

abstract：:Interaction between disease-microbiome associations and ageing has not been explored in detail. Here, using age/region-matched sub-sets, we analysed the gut microbiome differences across five major diseases in a multi-cohort dataset constituting more than 2500 individuals from 20 to 89 years old. We show that disease-...

journal_title：eLife

authors： Ghosh TS,Das M,Jeffery IB,O'Toole PW

更新日期：2020-03-11 00:00:00

abstract：:Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize the contribution of damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response t...

journal_title：eLife

authors： Atashpaz S,Samadi Shams S,Gonzalez JM,Sebestyén E,Arghavanifard N,Gnocchi A,Albers E,Minardi S,Faga G,Soffientini P,Allievi E,Cancila V,Bachi A,Fernández-Capetillo Ó,Tripodo C,Ferrari F,López-Contreras AJ,Costanzo V

更新日期：2020-03-12 00:00:00

abstract：:The transcription factor TCF7L1 is an embryonic stem cell signature gene that is upregulated in multiple aggressive cancer types, but its role in skin tumorigenesis has not yet been defined. Here we document TCF7L1 upregulation in skin squamous cell carcinoma (SCC) and demonstrate that TCF7L1 overexpression increases ...

journal_title：eLife

authors： Ku AT,Shaver TM,Rao AS,Howard JM,Rodriguez CN,Miao Q,Garcia G,Le D,Yang D,Borowiak M,Cohen DN,Chitsazzadeh V,Diwan AH,Tsai KY,Nguyen H

更新日期：2017-05-03 00:00:00

abstract：:The >800 human G protein-coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state- and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling make it challenging to understand their structure and function. Here, we devel...

journal_title：eLife

authors： Jones EM,Lubock NB,Venkatakrishnan AJ,Wang J,Tseng AM,Paggi JM,Latorraca NR,Cancilla D,Satyadi M,Davis JE,Babu MM,Dror RO,Kosuri S

更新日期：2020-10-21 00:00:00

abstract：:The membrane-bound transcription factor ATF6α plays a cytoprotective role in the unfolded protein response (UPR), required for cells to survive ER stress. Activation of ATF6α promotes cell survival in cancer models. We used cell-based screens to discover and develop Ceapins, a class of pyrazole amides, that block ATF6...

journal_title：eLife

authors： Gallagher CM,Garri C,Cain EL,Ang KK,Wilson CG,Chen S,Hearn BR,Jaishankar P,Aranda-Diaz A,Arkin MR,Renslo AR,Walter P

更新日期：2016-07-20 00:00:00