Abstract：
:Body fluids detected on commonly found crime scene substrates require extraction, purification and quantitation of DNA prior to amplification and generation of short tandem repeat (STR) DNA profiles. In this research Y-STR profiles were generated via direct amplification of blood and saliva deposited on 12 different substrates. These included cigarette butts, straws, grass, leaves, woodchips and seven different types of fabric. After depositing either 0.1 μL of blood or 0.5 μL of saliva, each substrate containing the dry body fluid stain was punched using a Harris 1.2 mm micro-punch. Each of these punched substrates, a total of 720 samples, containing minute amount of blood or saliva was either amplified directly without any pre-treatment, or was treated with one of the four washing reagents or buffer. In each of these five experimental groups the substrates containing the body fluid remained in the amplification reagent during the thermal cycling process. Each sample was amplified with the three direct Y-STR amplification kits; AmpFℓSTR(®) Yfiler(®) Direct, Yfiler(®) Plus Amplification Kits and the PowerPlex(®) Y23 System. Complete and concordant Y-STR profiles were successfully obtained from most of these 12 challenging crime scene objects when the stains were analyzed by at least one of the five experimental groups. The reagents and buffer were interchangeable among the three amplification kits, however, pre-treatment with these solutions did not appear to enhance the quality or the number of the full profiles generated with direct amplification. This study demonstrates that blood and saliva deposited on these simulated crime scene objects can be amplified directly.
journal_name
J Forensic Leg Medjournal_title
Journal of forensic and legal medicineauthors
Dargay A,Roy Rdoi
10.1016/j.jflm.2016.01.012subject
Has Abstractpub_date
2016-04-01 00:00:00pages
50-60eissn
1752-928Xissn
1878-7487pii
S1752-928X(16)00013-5journal_volume
39pub_type
杂志文章abstract：:We report a fatal case of streptococcal and meningococcal meningitis in a previously healthy 2-year-old child, a simultaneous co-infection of both pathogens that is poorly reported in the reviewed literature. The lack of a clinical diagnosis in addition to the medico-legal aspects arising from possible professional li...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2013.04.004
更新日期：2013-08-01 00:00:00
abstract：:Measurements of hand bones length have been shown to be sexually dimorphic in many nationalities. The aim of this study is to assess the accuracy of sex determination from the length of all metacarpals and phalanges of right and left hands using X-ray radiographs and to develop a discriminant formula that can be used ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2012.04.020
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abstract：:A comparative study was made investigating whether emergency room physicians, emergency room nurses, forensic physicians, and interns are competent in describing, recognising and determining the possible cause of injuries. The injury assessment scores varied from good--adequate--fail and remained blank in various part...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jcfm.2006.06.029
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abstract：:A case of poisoning with highly lipid soluble organophosphate compound, fenthion is reported in which cholinergic crisis recurred upto 25 days following a suicide attempt. Subcutaneous injection of fenthion in the antecubital fossa by the patient produced massive swelling, cellulitis and compartment syndrome of the le...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2007.07.008
更新日期：2008-05-01 00:00:00
abstract：:The number of people dependent on crack-cocaine in the UK has increased substantially in recent years. Some crack-cocaine users develop coarsening changes in the appearance of their hands after prolonged use of the drug. These changes have most often been recognized in females and include: (i) Perniosis with cold, num...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jcfm.2006.01.013
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abstract：:Air weapons whether rifles or pistols are, potentially, lethal weapons. The UK legislation is complex and yet little known to the public. Hunting with air weapons and the laws controlling those animals that are permitted to be shot with air weapons is even more labyrinthine due to the legal power limitations on the po...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2009.11.003
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abstract：:Magnetic resonance imaging (MRI) for forensic age estimation is among the research issues since it does not lead to radiation exposure. In this study, the ossification stage of the proximal humeral epiphysis was determined retrospectively in 178 male and 109 female individuals in the 12 to 30-year age group using 3.0 ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2020.102095
更新日期：2021-01-01 00:00:00
abstract：:Colchicum autumnale is commonly known as autumn crocus, and as 'gowri gedde' in the southern region of Karnataka State in South India. It contains an alkaloid called colchicine, which blocks the cell division by inhibiting mitosis. We present a sporadic case of suicidal plant poisoning wherein a 24-year-old man consum...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2011.04.008
更新日期：2011-08-01 00:00:00
abstract：:A 5-year-old boy with a chromosome-9 abnormality and multiple external and visceral malformations was found in cardiopulmonary arrest during a regular visit to the hospital; he did not respond to cardiopulmonary resuscitation and died. An odontoid process fracture and calcification and fibrosis of the muscles around t...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2018.11.011
更新日期：2019-02-01 00:00:00
abstract：INTRODUCTION:There are several studies about M1 type vehicle-pedestrian collision injury pattern, and based on them, there has been several changes in automobiles for pedestrian protection. However, the lack of sufficient studies about injury pattern in motorbikes-pedestrian collisions leads to a lack of optimization d...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2016.07.009
更新日期：2016-10-01 00:00:00
abstract：:Adipocere has a long history of frightening and fascinating mankind, from so called "incorruptible saints" to the famous "iceman" and its formation on human remains has long been known and feared. Adipocere formation replaces the putrefactive changes, when the body lies buried in shallow, moist, clay, soiled grave or ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章,评审
doi：10.1016/j.jflm.2009.07.004
更新日期：2009-11-01 00:00:00
abstract：:This study aims to provide descriptive data regarding male victims of sexual assault seen at the Centre for Victims of Sexual Assault in Copenhagen, Denmark. All 55 male victims attending the center in the time period of March 2001 until December 2010 underwent a standardized data collection. Data included information...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2016.06.007
更新日期：2016-10-01 00:00:00
abstract：:In the daily practice of external post-mortem examinations and dealing with surviving dependents, striking failures can be observed regularly, pointing to an increased demand for training of the various professional groups involved. In this study, the experience gained from using simulation mannequins and actors for p...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2020.102102
更新日期：2021-01-01 00:00:00
abstract：:This paper examines the problems faced by forensic physicians assessing overweight victim/survivors of sexual assault, using sample cases and reviewing the literature. This under-researched area is a minefield of practical difficulties and unanswered questions, with the need for solutions becoming more pressing as ove...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2012.07.013
更新日期：2013-05-01 00:00:00
abstract：:A man, who had a medical history of surgical neck clipping 30 years previously, died of traffic accident. The medico-legal autopsy showed subarachnoid hemorrhage and ruptured aneurysm on the lateral side of the clip. Microscopic examination showed the aneurysm was not to be regeneration, but a new de novo aneurysm. We...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2013.12.015
更新日期：2014-02-01 00:00:00
abstract：:The aim of this study was to compare the accuracy of Demirjian's four dental development methods for forensic age assessment in a South Australian population. The sample comprised orthopantomograms (OPGs) of 408 sub-adult individuals (211 male; 197 female) with an age range of 4.9-14.5 years. The OPGs were obtained fr...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2013.07.002
更新日期：2013-10-01 00:00:00
abstract：:Sudden cardiac deaths constitute a major health problem. Most cases are attributed to cardiomyopathies, coronary artery diseases and functional dysregulations. Sudden death in an adult due to a primitive cardiac tumor is a rare occurrence. In the following, we present a case of an adult male who died from an undiagnos...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2010.08.009
更新日期：2010-11-01 00:00:00
abstract：:Handprint in the scene of the crime is one of the most valuable clues in identification of the assailant. There are numerous studies on estimation of stature from direct measures of hand dimensions, but using a handprint instead, there is little research on it. So this study tried to focus on handprint as a tool used ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2015.05.007
更新日期：2015-08-01 00:00:00
abstract：:We report for the first time the lack of therapeutic effects after the switch from a brand formulation of allopurinol to a generic one. A 56-year-old man, with a 5 years history of well-treated gout arthropathy with allopurinol (Zyloric(®) 300 mg/die), developed acute gout arthropathy after the switch from the brand f...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2013.02.007
更新日期：2013-07-01 00:00:00
abstract：AIM:Infantile cerebral palsy (CP) severely affects the survival and quality of life of infants. CP is typically caused by multiple factors, leading to causal uncertainty of the role of medical errors in CP and resulting in frequent medical disputes. No relevant research exists on risk management and malpractice liabili...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2018.11.010
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abstract：:A case is presented of a fatal environmental accidental injuries of lightning. A pedestrian was struck by lightning. The macroscopic and microscopic lightning injuries are reviewed. ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2008.07.009
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abstract：INTRODUCTION:Effectiveness and safety assessment for kinetic energy (KE) bullets is of importance, considering numerous reports about excessive damage cases of KE bullets in recent years. Early study of KE impacting targets mainly focused on threshold penetration issue, but did not involve cavity evolution of target an...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2019.07.009
更新日期：2019-10-01 00:00:00
abstract：:To explore the CT characteristics of orbital blowout fracture, we reviewed 76 cases with orbital blowout fracture and analyzed their clinical forensic characteristics. The missed diagnosis rate of cranial CT was 26.3%, and plain X-ray was 47.4%. The orbital CT examination has advantages in diagnosing orbital blowout f...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2007.12.010
更新日期：2009-01-01 00:00:00
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journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2011.09.006
更新日期：2012-02-01 00:00:00
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journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2020.102007
更新日期：2020-08-01 00:00:00
abstract：:The antioxidant properties of the black and green tea are well known. The latent bloodstains are detectable by luminol. The bloodstains also can be cover up by drinks and foods containing the antioxidants; thus their presence can cause a decrease of the luminol light emission (false-negative results). The aim of this ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2013.03.027
更新日期：2013-08-01 00:00:00
abstract：:Medical examinations are dependent on combining communication with professional competence. In the development of a global multicultural community with the use of multiple languages, doctors have become increasingly dependent on language facilitation such as interpreting and translation. Despite professional studies, ...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2014.04.019
更新日期：2014-07-01 00:00:00
abstract：:Sexual violence is defined as any sexual act forced upon a person who did not give his or her consent. Our objective is to investigate the socio-demographic features, clinical correlates, criminal behaviour characteristics, and the level of penal responsibility of sexual offenders who were referred to forensic psychia...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2015.01.003
更新日期：2015-04-01 00:00:00
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journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2014.06.004
更新日期：2014-08-01 00:00:00
abstract：UNLABELLED:Falanga torture involves repetitive blunt trauma to the soles of the feet and typically leaves few detectable changes. Reduced elasticity in the heel pads has been reported as characteristic sequelae and palpatory testing of heel pad elasticity is therefore part of medicolegal assessment of alleged torture v...
journal_title：Journal of forensic and legal medicine
pub_type： 杂志文章
doi：10.1016/j.jflm.2008.03.003
更新日期：2008-10-01 00:00:00
