'Candidatus Phytoplasma cirsii', a novel taxon from creeping thistle [Cirsium arvense (L.) Scop].

Abstract:

:Creeping thistle [Cirsium arvense (L.) Scop.] and dahlia (Dahlia sp.) plants showing typical symptoms of phytoplasma infection including yellowing, stunting, inflorescence and proliferation, were sampled; the presence of phytoplasma was confirmed by standard PCR using universal primers. RFLP analysis allowed classification of the detected phytoplasma strains CirYS, CirYS1 and DahlP within the 16SrXI group, the unique restriction profile F2nR2 fragment obtained in silico by iPhyClassifier indicated that they belong to the new 16SrXI-E subgroup. Genetic analysis of the 16S rRNA gene revealed that the studied strains shared less than 97.5% similarity with all of the previously described 'Candidatus Phytoplasma' species. The closest relatives are 'Candidatus Phytoplasma cynodontis' and 'Candidatus Phytoplasma oryzae' with 96.8% and 96.6% similarity. All strains studied bear three specific regions in the 16S rRNA gene, discriminating them from the other phytoplasma species. Phylogenetic analysis of the 16S rRNA and secA genes confirmed this specificity, as the creeping thistle and dahlia phytoplasma strains clustered in a distinguishable lineage group. The uniqueness of the genetic analysis agrees with the biological characterization of the studied phytoplasma strains, their host range, and geographical distribution. The strains only infect dicotyledonous plants in Europe, contrary to their closest relatives. Based on their unique properties, it could be concluded that the studied phytoplasma strains represent a discrete group that is proposed as a novel taxon 'Candidatus Phytoplasma cirsii', with strain CirYS as a reference strain.

authors

Šafárˇová D,Zemánek T,Válová P,Navrátil M

doi

10.1099/ijsem.0.000937

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

1745-1753

issue

4

eissn

1466-5026

issn

1466-5034

journal_volume

66

pub_type

杂志文章
  • Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil.

    abstract::Forty strains isolated from soil taken from a hay meadow were assigned to the genus Dactylosporangium on the basis of colonial properties. 16S rRNA gene sequence analysis showed that the isolates formed a group that was most closely related to the type strain of Dactylosporangium aurantiacum, but well separated from o...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.016451-0

    authors: Kim BY,Stach JEM,Weon HY,Kwon SW,Goodfellow M

    更新日期:2010-08-01 00:00:00

  • Thalassomonas eurytherma sp. nov., a marine proteobacterium.

    abstract::Two Gram-staining-negative, aerobic, rod-shaped bacterial strains, designated Za6a-12(T) and Za6a-17, were isolated from seawater of the East China Sea. Cells of Za6a-12(T) and Za6a-17 were approximately 1.5-2.0 µm×0.5-0.7 µm and motile by a single polar flagellum. Strains grew optimally at pH 7.5-8.0, 28 °C, and in t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.058255-0

    authors: Sun C,Huo YY,Liu JJ,Pan J,Qi YZ,Zhang XQ,Zhang Y,Zheng G,Wu M

    更新日期:2014-06-01 00:00:00

  • A proposal for further integration of the cyanobacteria under the Bacteriological Code.

    abstract::This taxonomic note reviews the present status of the nomenclature of the cyanobacteria under the Bacteriological Code. No more than 13 names of cyanobacterial species have been proposed so far in the International Journal of Systematic and Evolutionary Microbiology (IJSEM)/International Journal of Systematic Bacterio...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.03008-0

    authors: Oren A

    更新日期:2004-09-01 00:00:00

  • Borrelia kurtenbachii sp. nov., a widely distributed member of the Borrelia burgdorferi sensu lato species complex in North America.

    abstract::Lyme borreliosis group spirochaetes are parasitic bacteria transmitted by vector ticks of the genus Ixodes and distributed mainly between 40° and 60° northern latitudes. Since Borrelia burgdorferi sensu stricto (hereinafter, B. burgdorferi) was described in the north-eastern USA during the early 1980s, an increasing d...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.054593-0

    authors: Margos G,Piesman J,Lane RS,Ogden NH,Sing A,Straubinger RK,Fingerle V

    更新日期:2014-01-01 00:00:00

  • Paenibacillus marchantiophytorum sp. nov., isolated from the liverwort Herbertus sendtneri.

    abstract::A Gram-stain-positive, aerobic, motile, rod-shaped, endospore-forming bacterium, designated strain R55T, was isolated from the liverwort Herbertus sendtneri growing at Gawalong glacier, Tibet, and characterized by using a polyphasic taxonomic approach. The major fatty acids of strain R55T were anteiso-C15 : 0, C16 : 0...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000789

    authors: Guo GN,Zhou X,Chen ZL,Yang ZW,Li XD,Li YH

    更新日期:2016-02-01 00:00:00

  • Micromonospora sediminis sp. nov., isolated from mangrove sediment.

    abstract::The novel actinomycete, strain CH3-3T, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus Micromonospora. It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose and rh...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001175

    authors: Phongsopitanun W,Kudo T,Ohkuma M,Pittayakhajonwut P,Suwanborirux K,Tanasupawat S

    更新日期:2016-08-01 00:00:00

  • Pseudoxanthobacter liyangensis sp. nov., isolated from dichlorodiphenyltrichloroethane-contaminated soil.

    abstract::An aerobic, Gram-stain negative, pale, rod-shaped, nitrogen-fixing bacterial strain, DDT-3(T), was isolated from dichlorodiphenyltrichloroethane-contaminated soil in Liyang, PR China. Strain DDT-3(T) grew at temperatures ranging from 20 to 40 °C (optimum 30-37 °C) and a pH of between 5.0 and 10.0 (optimum pH 7.0-8.0)....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.056507-0

    authors: Liu XM,Chen K,Meng C,Zhang L,Zhu JC,Huang X,Li SP,Jiang JD

    更新日期:2014-10-01 00:00:00

  • Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses.

    abstract::Four bacterial strains, designated ST18(T), HM244, HM250 and DI49, were isolated from the fresh faeces of four thoroughbred horses in Japan. Cells were Gram-positive, strictly anaerobic, catalase-negative, non-spore-forming, non-motile rods that occurred in chains. They were placed in the same subcluster based on 16S ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65543-0

    authors: Morita H,Shiratori C,Murakami M,Takami H,Toh H,Kato Y,Nakajima F,Takagi M,Akita H,Masaoka T,Hattori M

    更新日期:2008-12-01 00:00:00

  • Rhizomicrobium electricum sp. nov., a facultatively anaerobic, fermentative, prosthecate bacterium isolated from a cellulose-fed microbial fuel cell.

    abstract::A facultatively anaerobic, prosthecate bacterium, strain Mfc52(T), was isolated from a microbial fuel cell inoculated with soil and fed with cellulose as the sole fuel. Cells were Gram-negative, non-spore-forming, straight or slightly curved rods, and some of them had one or two polar prosthecae (stalks). Cells reprod...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.023580-0

    authors: Kodama Y,Watanabe K

    更新日期:2011-08-01 00:00:00

  • The cpcB-cpcA locus as a tool for the genetic characterization of the genus Arthrospira (Cyanobacteria): evidence for horizontal transfer.

    abstract::To investigate the genetic diversity of the genus Arthrospira and to compare it with other cyanobacteria, sequences of 670 nt from the phycocyanin operon were determined for 23 natural, cultivated or commercial strains of Arthrospira and compared with sequences from 20 other non-Arthrospira cyanobacterial strains. The...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-3-861

    authors: Manen JF,Falquet J

    更新日期:2002-05-01 00:00:00

  • Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon.

    abstract::A novel acidothermophilic archaeon, strain Ar-4(T), was isolated from a sulfuric hot spring in Tengchong, Yunnan, China. Cells of strain Ar-4(T) were Gram-staining-negative, irregular cocci and motile by means of flagella. Strain Ar-4(T) grew over a temperature range of 55-75 °C (optimum, 65 °C), a pH range of 2.5-5.5...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.026591-0

    authors: Liu LJ,You XY,Guo X,Liu SJ,Jiang CY

    更新日期:2011-10-01 00:00:00

  • Moniliella byzovii sp. nov., a chlamydospore-forming black yeast isolated from flowers.

    abstract::Yeasts of the genus Moniliella were isolated from 651 flower samples collected in Vietnam, using an enrichment medium containing 50 % glucose. Species of the genus Moniliella were found in 5 % of the samples and 54 strains were isolated. The strains were identified based on D1/D2 LSU rRNA gene sequences as M. megachil...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.049767-0

    authors: Thanh VN,Hien DD,Thom TT

    更新日期:2013-03-01 00:00:00

  • Polyphasic characterization of xanthomonads pathogenic to members of the Anacardiaceae and their relatedness to species of Xanthomonas.

    abstract::We have used amplified fragment length polymorphism (AFLP), multilocus sequence analysis (MLSA) and DNA-DNA hybridization for genotypic classification of Xanthomonas pathovars associated with the plant family Anacardiaceae. AFLP and MLSA results showed congruent phylogenetic relationships of the pathovar mangiferaeind...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65453-0

    authors: Ah-You N,Gagnevin L,Grimont PA,Brisse S,Nesme X,Chiroleu F,Bui Thi Ngoc L,Jouen E,Lefeuvre P,Vernière C,Pruvost O

    更新日期:2009-02-01 00:00:00

  • Herminiimonas glaciei sp. nov., a novel ultramicrobacterium from 3042 m deep Greenland glacial ice.

    abstract::A Gram-negative ultramicrobacterium (designated strain UMB49(T)) was isolated from a 120,000-year-old, 3,042 m deep Greenland glacier ice core using a 0.2 mum filtration enrichment procedure. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this strain belonged to the genus Herminiimonas of the famil...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.001685-0

    authors: Loveland-Curtze J,Miteva VI,Brenchley JE

    更新日期:2009-06-01 00:00:00

  • Erythrobacter aquimixticola sp. nov., isolated from the junction between the ocean and a freshwater spring.

    abstract::A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated JSSK-14T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-14T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002055

    authors: Park S,Jung YT,Choi SJ,Yoon JH

    更新日期:2017-08-01 00:00:00

  • Methanofollis ethanolicus sp. nov., an ethanol-utilizing methanogen isolated from a lotus field.

    abstract::A novel methane-producing archaeon, designated strain HASU(T), was isolated from a lotus field. Cells were Gram-negative, non-motile, irregular cocci, 2-3 mum in diameter, and occurred singly. Growth was observed at 15-40 degrees C (optimum, 37 degrees C) and pH 6.5-7.5 (optimum, pH 7.0). The G+C content of the genomi...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.003731-0

    authors: Imachi H,Sakai S,Nagai H,Yamaguchi T,Takai K

    更新日期:2009-04-01 00:00:00

  • Massilia lurida sp. nov., isolated from soil.

    abstract::A bacterial isolate, designated strain D5(T), was isolated from a soil sample collected from the Inner Mongolia Autonomous Region, China, and subjected to a taxonomic investigation using a polyphasic approach. Strain D5(T) was aerobic, Gram-stain-negative, rod-shaped and motile. Strain D5(T) fell within the evolutiona...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.047068-0

    authors: Luo X,Xie Q,Wang J,Pang H,Fan J,Zhang J

    更新日期:2013-06-01 00:00:00

  • Microbacterium hydrothermale sp. nov., an actinobacterium isolated from hydrothermal sediment.

    abstract::A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, strain 0704C9-2(T), was isolated from hydrothermal sediment of the Indian Ocean. The organism grew with 0-5% (w/v) NaCl and at 10-37 °C, with optimal growth occurring with 1% NaCl and at 28-30 °C. Comparative 16S rRNA gene sequence analysis revealed tha...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.061697-0

    authors: Zhang Y,Ren H,Zhang G

    更新日期:2014-10-01 00:00:00

  • Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagu

    abstract::A taxonomic study of a novel marine, heterotrophic, non-gliding, halotolerant and light-pink-pigmented bacterium was carried out using a polyphasic approach. 16S rRNA gene sequence analysis revealed that strain KMM 6241(T) is a member of the phylum Bacteroidetes. Strain KMM 6241(T) formed a cluster with the genera Alg...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65073-0

    authors: Nedashkovskaya OI,Kim SB,Kwon KK,Shin DS,Luo X,Kim SJ,Mikhailov VV

    更新日期:2007-09-01 00:00:00

  • Kineococcus terrestris sp. nov. and Kineococcus aureolus sp. nov., isolated from saline sediment.

    abstract::Two novel actinobacteria, designated YIM 121936T and YIM 121940T, were isolated from alkaline sediment in Yuanjiang, China. The cells of the novel strains were Gram-stain-positive, aerobic, motile, non-spore-forming and coccus-shaped. The two strains both contained meso-diaminopimelic acid as the diagnostic diamino ac...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002380

    authors: Xu FJ,Li QY,Li GD,Chen X,Jiang Y,Jiang CL

    更新日期:2017-11-01 00:00:00

  • Terrimonas pekingensis sp. nov., isolated from bulking sludge, and emended descriptions of the genus Terrimonas, Terrimonas ferruginea, Terrimonas lutea and Terrimonas aquatica.

    abstract::A Gram-negative, strictly aerobic, non-motile and non-spore-forming rod that produced white, viscous colonies, designated QH(T), was isolated from bulking sludge collected from a municipal wastewater treatment plant in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QH(T) belo...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.036848-0

    authors: Jin D,Wang P,Bai Z,Jin B,Yu Z,Wang X,Zhuang G,Zhang H

    更新日期:2013-05-01 00:00:00

  • Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L.

    abstract::An endophytic actinomycete, strain R10-82(T), isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82(T) produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores. The major cellular fatty ac...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.057885-0

    authors: Mingma R,Duangmal K,Trakulnaleamsai S,Thamchaipenet A,Matsumoto A,Takahashi Y

    更新日期:2014-04-01 00:00:00

  • Persicitalea jodogahamensis gen. nov., sp. nov., a marine bacterium of the family 'Flexibacteraceae', isolated from seawater in Japan.

    abstract::An obligately aerobic, Gram-negative, non-motile, pale-pink-pigmented, rod-shaped strain, designated Shu-9-SY12-35C(T), was isolated from seawater in Jodogahama, Iwate, Japan, and was subjected to a polyphasic taxonomic examination. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isol...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64792-0

    authors: Yoon J,Ishikawa S,Kasai H,Yokota A

    更新日期:2007-05-01 00:00:00

  • Ogataea haglerorum sp. nov., a novel member of the species complex, Ogataea (Hansenula) polymorpha.

    abstract::Three strains representing a novel species of the Ogataea clade were isolated by W. T. Starmer and H. J. Phaff from rotting tissue of Opuntia phaeacantha in Arizona, USA. Analyses of the sequences of the D1/D2 LSU rRNA gene, ITS1-5.8S-ITS2, and translation elongation factor-1α (EF-1 α) showed that this novel species b...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002012

    authors: Naumov GI,Naumova ES,Lee CF

    更新日期:2017-07-01 00:00:00

  • Helcococcus sueciensis sp. nov., isolated from a human wound.

    abstract::A previously undescribed, Gram-positive, catalase-negative, coccus-shaped organism that originated from a human wound was subjected to taxonomic study. On the basis of its cellular morphology and the results of biochemical testing, the unknown organism was identified tentatively as a member of the genus Helcococcus, b...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63077-0

    authors: Collins MD,Falsen E,Brownlee K,Lawson PA

    更新日期:2004-09-01 00:00:00

  • Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate.

    abstract::A strain of a novel non-chromogenic mycobacterium was isolated from synovial tissue from a 68-year-old female with bursitis of her right elbow. The slowly growing strain had a unique PCR-restriction enzyme analysis (PRA) profile of the hsp65 gene and 16S rRNA gene sequence in comparison with other mycobacterium specie...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-6-2135

    authors: Turenne C,Chedore P,Wolfe J,Jamieson F,Broukhanski G,May K,Kabani A

    更新日期:2002-11-01 00:00:00

  • Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis.

    abstract::A new Pseudomonas species, for which the name Pseudomonas plecoglossicida is proposed, was isolated from cultured ayu (Plecoglossus altivelis) with bacterial haemorrhagic ascites. The causative agent was similar to Pseudomonas putida biovar A in its phenotypic characteristics and on the basis of 16S rRNA gene sequence...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-1-83

    authors: Nishimori E,Kita-Tsukamoto K,Wakabayashi H

    更新日期:2000-01-01 00:00:00

  • Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador.

    abstract::In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004(T)) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represe...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.047555-0

    authors: James SA,Carvajal Barriga EJ,Barahona PP,Cross K,Bond CJ,Roberts IN

    更新日期:2013-01-01 00:00:00

  • Nocardioides iriomotensis sp. nov., an actinobacterium isolated from forest soil.

    abstract::An actinomycete strain, designated IR27-S3(T), was isolated from a forest soil sample collected from Iriomote Island, Okinawa, Japan. Cells of the isolate were Gram-stain-positive, aerobic, non-sporulating, non-motile coccoids or short rods. The strain grew in the presence of 0-7 % (w/v) NaCl, at pH 6-8 and at 12-37 °...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.025080-0

    authors: Yamamura H,Ohkubo SY,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Hayakawa M

    更新日期:2011-09-01 00:00:00

  • Ulvibacter antarcticus sp. nov., isolated from Antarctic coastal seawater.

    abstract::A seawater bacterium, designated IMCC3101(T), was isolated from Antarctic coastal seawater. The strain was Gram-negative, chemoheterotrophic, obligately aerobic, pigmented dark yellow (flexirubin-type pigments) and devoid of gliding and flagellar motility. On the basis of 16S rRNA gene sequence comparisons, the most c...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65265-0

    authors: Choi TH,Lee HK,Lee K,Cho JC

    更新日期:2007-12-01 00:00:00