Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate.

Abstract:

:A strain of a novel non-chromogenic mycobacterium was isolated from synovial tissue from a 68-year-old female with bursitis of her right elbow. The slowly growing strain had a unique PCR-restriction enzyme analysis (PRA) profile of the hsp65 gene and 16S rRNA gene sequence in comparison with other mycobacterium species. The most closely related species, as determined by 16S rRNA gene sequence analysis, are Mycobacterium malmoense, Mycobacterium marinum, Mycobacterium ulcerans and members of the Mycobacterium tuberculosis complex. The HPLC and biochemical profiles resembled those of Mycobacterium gastri, although differences were noted in the peak-height ratio of the HPLC pattern and the nitrate and pyrazinamidase tests. On the basis of PRA, HPLC, biochemical and 16S rRNA gene sequence analyses, the name Mycobacterium lacus sp. nov. is proposed for this potential pathogen. The type strain is strain NRCM 00-255(T) (= ATCC BAA-323(T) = DSM 44577(T)).

authors

Turenne C,Chedore P,Wolfe J,Jamieson F,Broukhanski G,May K,Kabani A

doi

10.1099/00207713-52-6-2135

keywords:

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

2135-2140

issue

Pt 6

eissn

1466-5026

issn

1466-5034

journal_volume

52

pub_type

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