Nocardioides iriomotensis sp. nov., an actinobacterium isolated from forest soil.

Abstract:

:An actinomycete strain, designated IR27-S3(T), was isolated from a forest soil sample collected from Iriomote Island, Okinawa, Japan. Cells of the isolate were Gram-stain-positive, aerobic, non-sporulating, non-motile coccoids or short rods. The strain grew in the presence of 0-7 % (w/v) NaCl, at pH 6-8 and at 12-37 °C, with optimum growth at 30 °C. Chemotaxonomically, the strain contained LL-diaminopimelic acid as the diagnostic diamino acid and MK-8(H₄) as the predominant menaquinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids were iso-C₁₆:₀, C₁₇:₁ cis-9, C₁₅:₀ and iso-C₁₅:₀. The DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence analysis, strain IR27-S3(T) was closely related to Nocardioides mesophilus MSL-22(T) (98.1 % 16S rRNA gene sequence similarity), Marmoricola bigeumensis MSL-05(T) (97.2 %) and Nocardioides jensenii DSM 20641(T) (96.5 %). On the basis of fatty acid analysis, phylogenetic analysis and phenotypic data, the isolate should be classified in a novel species of the genus Nocardioides, for which the name Nocardioides iriomotensis sp. nov. is proposed. The type strain is IR27-S3(T) ( = NBRC 105384(T)  = KACC 14926(T)).

authors

Yamamura H,Ohkubo SY,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Hayakawa M

doi

10.1099/ijs.0.025080-0

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

2205-2209

issue

Pt 9

eissn

1466-5026

issn

1466-5034

journal_volume

61

pub_type

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