Abstract:
:A novel acidothermophilic archaeon, strain Ar-4(T), was isolated from a sulfuric hot spring in Tengchong, Yunnan, China. Cells of strain Ar-4(T) were Gram-staining-negative, irregular cocci and motile by means of flagella. Strain Ar-4(T) grew over a temperature range of 55-75 °C (optimum, 65 °C), a pH range of 2.5-5.5 (optimum, pH 3.5) and a NaCl concentration range of 0-1 % (w/v). The novel strain was aerobic and facultatively chemolithoautotrophic. The strain could extract metal ions from sulfidic ore. It was also able to oxidize reduced sulfur compounds. In addition, it was able to use heterogeneous organic materials for organotrophic growth. The main cellular lipids were calditoglycerocaldarchaeol (CGTE) and caldarchaeol (DGTE). The DNA G+C content of the strain was 40.2 mol%. Analysis of 16S rRNA gene sequences showed that strain Ar-4(T) was phylogenetically related to members of the genus Metallosphaera and had sequence similarities of 97.7 %, 97.0 % and 96.8 % with Metallosphaera hakonensis DSM 7519(T), Metallosphaera sedula DSM 5348(T) and Metallosphaera prunae DSM 10039(T), respectively. Strain Ar-4(T) showed DNA-DNA relatedness values of 47.5 %, 30.8 % and 29.1 % with M. hakonensis DSM 7519(T), M. sedula DSM 5348(T) and M. prunae DSM 10039(T), respectively. The differences in cell motility, the temperature and pH ranges for growth, the ability to utilize carbon sources, the DNA G+C content, and the low DNA-DNA relatedness values distinguished strain Ar-4(T) from recognized species of the genus Metallosphaera. On the basis of these results, it was concluded that strain Ar-4(T) represents a novel species of the genus Metallosphaera, for which the name Metallosphaera cuprina is proposed. The type strain is Ar-4(T) ( = JCM 15769(T) = CGMCC 1.7082(T)).
journal_name
Int J Syst Evol Microbiolauthors
Liu LJ,You XY,Guo X,Liu SJ,Jiang CYdoi
10.1099/ijs.0.026591-0subject
Has Abstractpub_date
2011-10-01 00:00:00pages
2395-2400issue
Pt 10eissn
1466-5026issn
1466-5034journal_volume
61pub_type
杂志文章abstract::A recent taxonomic study confirmed the synonymy of Rhodococcus equi (Magnusson 1923) Goodfellow and Alderson 1977 and Corynebacterium hoagii (Morse 1912) Eberson 1918. As a result, both R. equi and C. hoagii were reclassified as Rhodococcus hoagii comb. nov. in application of the principle of priority of the Prokaryot...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.004090
更新日期:2020-05-01 00:00:00
abstract::This listing of names of prokaryotes published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol41 (1991), p. 185]. The names given herein ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001168
更新日期:2016-08-01 00:00:00
abstract::A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach. Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003718
更新日期:2020-01-01 00:00:00
abstract::The taxonomic status of six strains received as Streptomyces hygroscopicus was established using a polyphasic approach. The organisms had phenotypic and morphological properties consistent with their classification in the genus Streptomyces. Almost complete 16S rRNA gene sequences of the strains were determined and al...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65408-0
更新日期:2008-06-01 00:00:00
abstract::A Gram-positive-staining, filamentous bacterial strain that developed cylindrical sporangia containing four oval- to rod-shaped spores at the ends of short sporangiophores on branched aerial mycelium was isolated from tropical rainforest soil near a hot spring. The cell-wall peptidoglycan contained meso-diaminopimelic...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.003228-0
更新日期:2009-05-01 00:00:00
abstract::Actinomycete strain YIM 90002T (= CCTCC 99003T = CCRC 16284T) was isolated from a soil sample collected from a salt lake in the west of China. The aerial mycelium of this organism is well developed but not fragmented and, at maturity, forms short chains of spores. Spores in short chains are oval- to rod-shaped and hav...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-2-357
更新日期:2001-03-01 00:00:00
abstract::A novel aerobic, non-motile, Gram-negative bacterium, designated AS06/20a(T), was isolated from coastal waters of the Adriatic Sea and subjected to polyphasic taxonomic analysis. Cells were rod-shaped and formed non-pigmented punctiform colonies on agar plates. The novel isolate grew heterotrophically on a variety of ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65446-0
更新日期:2008-10-01 00:00:00
abstract::A taxonomic study was carried out on a Gram-stain-negative bacterium, namely strain ANRC-JH13T, isolated from a sediment sample collected at Jasper beach, adjacent to Fildes Peninsula, Antarctica. Cells of strain ANRC-JH13T were non-spore-forming rods and motile by the way of flagellum. Strain ANRC-JH13T was facultati...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003359
更新日期:2019-06-01 00:00:00
abstract::The taxonomic position of three bacterial strains, Asr22-19T, NBRC 101035 and NBRC 101041, isolated from shellfish in Japan, was determined by using a polyphasic taxonomic approach. The strains were facultatively anaerobic, motile by gliding and Gram-staining-negative slender rods. Their major respiratory quinone was ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.016113-0
更新日期:2010-08-01 00:00:00
abstract::A Gram-stain-negative, oxidase-negative, catalase-positive, facultative anaerobe, designated XJ16T, was isolated from a marine solar saltern on the coast of Weihai, China. Cells of strain XJ16T were long and rod-shaped. The colonies were ochre in colour and were able to reduce nitrate to nitrite. Optimal growth occurr...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001718
更新日期:2017-04-01 00:00:00
abstract::A Gram-positive, non-motile, rod- or coccoid-shaped Microbacterium-like bacterium, designated strain DS-66(T), was isolated from soil of Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-66(T) grew optimally at 30 degrees C and pH 6.5-7.0 in the presence of 0.5-1...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007591-0
更新日期:2009-07-01 00:00:00
abstract::The fluorescent amplified fragment length polymorphism (FAFLP) groups A5 (21 isolates), A8 (6 isolates) and A23 (3 isolates) distinguished in an earlier paper (Thompson et al., Syst Appl Microbiol 24, 520-538, 2001) were examined in more depth. These three groups were phylogenetically related to Vibrio tubiashii, but ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.02447-0
更新日期:2003-01-01 00:00:00
abstract::A Gram-stain-positive, ovoid, lactic acid bacterium, strain LMG 27676T, was isolated from a spoiled sous-vide-cooked rutabaga. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc kimchii and Leuconostoc miyukkimchii as the nearest neighbours (99.1 and 98.8...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.000305
更新日期:2015-08-01 00:00:00
abstract::A novel, mesophilic, Gram-negative bacterium was isolated from an anaerobic digestor for municipal wastewater. The bacterium degraded adipate in the presence of sulfate, sulfite, thiosulfate and elemental sulfur. (E)-2-Hexenedioate accumulated transiently in the degradation of adipate. (E)-2-Hexenedioate, (E)-3-hexene...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-50-2-639
更新日期:2000-03-01 00:00:00
abstract::One Gram-stain-negative, aerobic, motile bacterial strain, 3-7T, was isolated from symptomatic canker bark tissue of Populus × euramericana. 16S rRNA gene sequence data revealed that the novel isolate shared highest similarity with Sphingomonas panacis DCY99T (98.8 %), and <96.5 % sequence similarity with all other sp...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003841
更新日期:2020-02-01 00:00:00
abstract::A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium designated strain C605018/01/1T isolated from a milk sample collected from the udder of a cow at post mortem. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacte...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.004230
更新日期:2020-07-01 00:00:00
abstract::Recent examination of the relationships of the dry necrosis-inducing (necrogenic) erwinias using 16S rDNA sequences demonstrated that these bacteria comprise a polyphyletic group and, therefore, have been subdivided into three distinct genera, Erwinia, Brenneria and Pectobacterium, with the classical 'amylovora' group...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-50-6-2057
更新日期:2000-11-01 00:00:00
abstract::Strain 2327T, first cultured from vertebral abscesses of green iguanas (Iguana iguana) collected in Florida, USA, was readily distinguished from all previously described mollicutes by 16S rRNA gene sequence comparisons. Strain 2327T lacks a cell wall, ferments glucose, does not hydrolyse arginine, aesculin or urea and...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.63852-0
更新日期:2006-04-01 00:00:00
abstract::Strain 7SM30(T)(,) an aerobic marine, Gram-negative, heterotrophic and yellow- to orange-pigmented bacterium isolated from seawater from Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that the isolate represented a novel lineage within the family Flavobac...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.020875-0
更新日期:2010-12-01 00:00:00
abstract::A yellow-pigmented, Gram-negative, rod-shaped, strictly aerobic bacterium (strain UST050418-085(T)) was isolated from the surface of a marine sponge, Myxilla incrustans, at Friday Harbor, WA, USA. The DNA G+C content of this strain was 34.6 mol%. The predominant fatty acids were i15 : 0, a15 : 0, i15 : 1, i16 : 0, i17...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64345-0
更新日期:2006-08-01 00:00:00
abstract::In 1911, Sir Arnold Theiler isolated and described a parasite that was very similar to Anaplasma marginale but which was more centrally located within the erythrocytes of the host cells, and was much less pathogenic than A. marginale. He named the parasite A. marginale variety centrale. The name Anaplasma centrale, re...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002832
更新日期:2018-08-01 00:00:00
abstract::A novel actinomycete, designated WYY166T, was isolated from the rhizosphere of Suaeda australis Moq. collected in Dongfang, PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on its 16S rRNA gene referred strain WYY166T to the genus Nonomuraea, and...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.004377
更新日期:2020-09-01 00:00:00
abstract::An anaerobic, thermophilic bacterium, strain SET IS-9(T), was isolated from an Icelandic hot spring. Cells of strain SET IS-9(T) are short, slightly curved, motile rods. The strain grows chemolithotrophically on CO, producing equimolar quantities of H(2) and CO(2). It also grows fermentatively on lactate or pyruvate i...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.030288-0
更新日期:2011-10-01 00:00:00
abstract::Two actinomycete strains were isolated from within the fynbos-rich area surrounded by the horseracing track at Kenilworth Racecourse in Cape Town, South Africa. Rapid molecular identification indicated that the isolates belonged to the family Micromonosporaceae. Based on 16S rRNA gene sequence blast analysis, the isol...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.042929-0
更新日期:2013-03-01 00:00:00
abstract::Strain RK1(T), a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium was isolated from a hexachlorocyclohexane (HCH) dumpsite, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RK1(T) belongs to the family Sphingobacteriaceae and showed highest sequence similarity to Parapedobac...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.069104-0
更新日期:2015-01-01 00:00:00
abstract::Strain 7SM29T, an aerobic marine gammaproteobacterium isolated from seawater from Castellón, Spain, was characterized by classical phenotyping, chemotaxonomy and 16S rRNA gene sequence analysis. Strain 7SM29T was found to be closely related to strains in the genus Haliea and to Congregibacter litoralis KT71T, with whi...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.017061-0
更新日期:2010-08-01 00:00:00
abstract::Seven spore-forming, nitrogen-fixing bacterial isolates from spruce forest humus in Finland were studied using the polyphasic approach. PCR amplification of 16S rRNA gene fragment with specific primers showed that the isolates were members of Paenibacillus. Levels of 16S rDNA similarity between the isolates were 97.3-...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-2-535
更新日期:2001-03-01 00:00:00
abstract::A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209T, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002648
更新日期:2018-04-01 00:00:00
abstract::A Gram-negative, rod-shaped, motile and aerobic bacterium, designated strain HJ51(T), was isolated from a seawater sample from the East Sea, near South Korea. The isolate grew slowly at 4 °C, was able to grow at 40 °C, required NaCl and grew optimally at pH 6.5-7.0. The G+C content of the genomic DNA was 41.8 mol%. Th...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.022541-0
更新日期:2011-02-01 00:00:00
abstract::The taxonomic position of the halophilic actinobacterial strain, HS05-03T, isolated from solar saltern soil, was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence of the strain showed that it formed a distinct evolutionary lineage in the genus Actinopolyspora. The organi...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000926
更新日期:2016-04-01 00:00:00