Abstract:
:Seven spore-forming, nitrogen-fixing bacterial isolates from spruce forest humus in Finland were studied using the polyphasic approach. PCR amplification of 16S rRNA gene fragment with specific primers showed that the isolates were members of Paenibacillus. Levels of 16S rDNA similarity between the isolates were 97.3-100.0% and those between the isolates and other Paenibacillus species were 90.3-96.5%. The highest similarities were observed with Paenibacillus azotofixans and Paenibacillus durus. Ribotyping with EcoRI and PvuII restriction showed a high diversity in the Paenibacillus species and distinguished the isolates from these closely related species. The main whole-cell fatty acids were anteiso-C15:0 (33-48%), straight-chain C14:0 (7-21%) and C16:0 (9-20%), and iso-C15:0 (6-15%). Electron microscopy revealed a unique striped morphology of the spore surfaces. Based on phylogenetic inference and phenotypic and chemotaxonomic characteristics, these isolates are proposed as a new species, Paenibacillus borealis sp. nov., the type strain of which is KK19T (= DSM 13188T = CCUG 43137T).
journal_name
Int J Syst Evol Microbiolauthors
Elo S,Suominen I,Kämpfer P,Juhanoja J,Salkinoja-Salonen M,Haahtela Kdoi
10.1099/00207713-51-2-535keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
535-545issue
Pt 2eissn
1466-5026issn
1466-5034journal_volume
51pub_type
杂志文章abstract::A Gram-positive actinobacterium, strain IV-75(T), was isolated by using R2A agar from the ultrapure water system of a power plant in Hungary. The strain exhibited a rod-coccus cell cycle, and was strictly aerobic, non-motile, catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis revealed that strain ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.032672-0
更新日期:2012-03-01 00:00:00
abstract::Strain CCP-7T, isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain CCP-7T formed a phylogenetic lineage in the genus Sphingomonas. Strain CCP-7T was mo...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003455
更新日期:2019-07-01 00:00:00
abstract::The National Antimicrobial Resistance Monitoring System Laboratory at the Centers for Disease Control and Prevention (CDC) isolated two enterococcus-like strains that were referred to the CDC Streptococcus Laboratory for further identification. The isolates were recovered from human stool samples collected on differen...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64103-0
更新日期:2006-07-01 00:00:00
abstract::Genome comparisons based on average nucleotide identity (ANI) values of four strains currently classified as Polynucleobacter necessarius subsp. asymbioticus resulted in ANI values of 75.7-78.4 %, suggesting that each of those strains represents a separate species. The species P. necessarius was proposed by Heckmann a...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001073
更新日期:2016-08-01 00:00:00
abstract::A non-motile, straight-rod-shaped, Gram-stain-positive and facultative anaerobic bacterium (i.e., strain G1T) was isolated from production waters from an Algerian oilfield. Growth was observed in the presence of 0.3-3.5 % (w/v) NaCl, at 20-50 °C and at pH 6.0-9.0. Results of phylogenetic analyses based on 16S rRNA gen...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.004434
更新日期:2020-12-01 00:00:00
abstract::A pink-pigmented, non-motile, coccoid bacterial strain, designated G3-6-20(T), was isolated from a soil sample collected in the Grove Mountains, East Antarctica. This strain was resistant to UV irradiation (810 J m(-2)) and slightly more sensitive to desiccation as compared with Deinococcus radiodurans. Phylogenetic a...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.066324-0
更新日期:2015-02-01 00:00:00
abstract::A novel bacterium, designated strain 5YN1-3(T), was isolated from wetland peat collected from Yongneup, Korea. The bacterium was facultatively anaerobic, Gram-negative, yellow-coloured, rod-shaped, mesophilic and motile with one polar flagellum. The strain grew optimally at 30 degrees C, at pH 6.0-9.0 and with 0-1 % N...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.002840-0
更新日期:2009-05-01 00:00:00
abstract::In the course of a taxonomic study describing novel species of the genus Enterobacter it was found that the 16S rRNA gene sequence of the type strain of Enterobacter siamensis, obtained both directly from the authors of the publication on Enterobacter siamensis and from the Korean Collection for Type Cultures (C2361T ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000777
更新日期:2016-01-01 00:00:00
abstract::Two Gram-stain-negative, oxidase-negative, catalase-positive, aerobic and coccus-shaped bacterial strains, KSY3-6T and JSH6-18, were isolated from soil in South Korea. Strains KSY3-6T and JSH6-18 showed high resistance to gamma-ray and UVC irradiation. The 16S rRNA gene sequences of strains KSY3-6T and JSH6-18 showed ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001473
更新日期:2016-12-01 00:00:00
abstract::A novel, Gram-stain-negative, facultatively anaerobic, halophilic bacterium, designated strain Q1UT, was isolated from a sediment sample collected from Qinghai Lake, PR China. The cells of the strain were short rod-shaped (0.2-0.3×0.6-2.5 µm) and non-motile. Strain Q1UT formed yellowish colonies and grew at temperatur...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001413
更新日期:2016-11-01 00:00:00
abstract::A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110T, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China. Cells were aerobic, motile with peritrichous flagella and rod-shaped. The strain grew at 15-37 °C (optimum, 28 °C), at 0-3 %(w...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003328
更新日期:2019-05-01 00:00:00
abstract::A Gram-negative bacterium, designated strain EMB71(T), was isolated from activated sludge used for enhanced biological phosphorus removal in a sequencing batch reactor. The cells of the isolate were facultatively aerobic, motile rods with single polar flagella. Growth was observed to occur at 15-35 degrees C (optimall...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64629-0
更新日期:2007-05-01 00:00:00
abstract::Eight strains of symbiotic bacteria from root nodules of local races of cowpea (Vigna unguiculata) and Bambara groundnut (Vigna subterranea) grown on subsistence farmers' fields in the Kavango region, Namibia, were previously characterized and identified as a novel group within the genus Bradyrhizobium. To clarify the...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000666
更新日期:2015-12-01 00:00:00
abstract::Pectobacterium isolates SS95T, SS54 and SS56 were collected from a potato field in the Chiniot district in the plains of the Punjab province, Pakistan. Sequencing of the gapA barcode revealed that these strains belong to a novel phylogenetic group separated from P.ectobacterium wasabiae and Pectobacterium parmentieri ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003029
更新日期:2018-11-01 00:00:00
abstract::A novel actinobacterium, designated strain NEAU-QY2T, was isolated from the leaves of Sonchus oleraceus L. specimen, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism formed single spores with rough surfaces on subs...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000668
更新日期:2015-12-01 00:00:00
abstract::A facultatively anaerobic, alkaliphilic, spore-forming, Gram-positive-staining rod, designated Y1(T), was isolated under strictly anaerobic conditions from sediment of a soda lake in Jilin province, China. The strain was not dependent on Na(+) but was highly halotolerant and grew optimally in medium JY with 0.5 M Na(+...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.018259-0
更新日期:2010-11-01 00:00:00
abstract::Five strains of the filamentous bacterium 'Nostocoida limicola' III were successfully isolated into pure culture from samples of activated sludge biomass from five plants in Australia. 16S rRNA gene sequence analyses showed that all isolates were members of the Planctomycetales, most closely related to Isosphaera pall...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-1-195
更新日期:2001-01-01 00:00:00
abstract::A Gram-stain-positive, aerobic bacterium, designated strain BZ41(T), was isolated from hydrocarbon-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ41(T) was related to members of the genus Agromyces and showed highest similarity with the type strain of Agromyces ramosus (...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.017186-0
更新日期:2010-10-01 00:00:00
abstract::The taxonomic position of Sphingobacterium antarcticum has been revised by means of 16S rRNA gene sequences, DNA-DNA hybridization, and phenotypic and chemotaxonomic characteristics. All data previously reported, as well as the results of the present phylogenetic analysis, support that Sphingobacterium antarcticum is ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.054965-0
更新日期:2014-03-01 00:00:00
abstract::Two yeast strains (FMCC Y-1(T) and FMCC Y-2) were recovered during a survey of the yeast biota associated with fermenting black olives, collected from an olive tree (Olea europaea L. cv. 'Conservolea') orchard in Central Greece. Phylogenetic analysis based on rRNA gene sequences (18S, 26S, and 5.8S-ITS) indicated that...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.012955-0
更新日期:2010-05-01 00:00:00
abstract::A novel anamorphic species of yeast belonging to the genus Candida was isolated from tar in Canada. Morphological and physiological observations, as well as phylogenetic analyses, were performed. Conidiophores were produced, were usually short and had sympodial growth, numerous bud scars and a rachis-like structure. T...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-51-6-2171
更新日期:2001-11-01 00:00:00
abstract::An iron-reducing, moderately thermophilic, acidophilic actinobacterium, strain IC-180(T), isolated from a solfataric field in Hakone, Japan, was subjected to polyphasic taxonomic analysis. Strain IC-180(T) was a motile, short rod-shaped, Gram-positive bacterium that was able to grow at temperatures of 35-58 °C (optima...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.023044-0
更新日期:2011-06-01 00:00:00
abstract::A novel strain, designated MRN461(T), was isolated from a marine sponge in Micronesia. The 16S rRNA gene sequence of the isolate showed 95.6 % similarity with that of 'Microscilla furvescens' IFO (now NBRC) 15994. Phylogenetic analysis revealed that the isolate and 'Microscilla furvescens' IFO 15994 formed a distinct ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.004317-0
更新日期:2009-05-01 00:00:00
abstract::Strain 6GN-30T, a Gram-stain-negative, aerobic, non-spore-forming, rod-shaped, motile bacterium was isolated from Ephedra sinica roots in the Kumtag Desert. On the basis of the 16S rRNA gene sequence, the isolate represented a member of the genus Mesorhizobium of the family Phyllobacteriaceae. The results of a phyloge...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003044
更新日期:2018-11-01 00:00:00
abstract::A methanogenic archaeon, strain HC-2(T), was isolated from a deep diatomaceous shale formation. The strain grew on methanol, monomethylamine, dimethylamine, trimethylamine and dimethylsulphide, but not on acetate, H2/CO2, formate, 2-propanol, 2-butanol or cyclopentanol. Cells were Gram-stain-negative, non-motile, and ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.000072
更新日期:2015-04-01 00:00:00
abstract::A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RA4-7T, was isolated from the gut of an abalone (Haliotis discus hannai) collected from the sea around Jeju island, South Korea, and subjected to a polyphasic taxonomic study. RA4-7T grew optimally at 25 °C, at pH ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001557
更新日期:2016-12-01 00:00:00
abstract::A Gram-stain-negative, aerobic, non-motile, rod-shaped, flexirubin-producing bacterium, designated strain CC-CZW010T, was isolated from the edible sea urchin Tripneustes gratilla in Penghu Island, Taiwan. The isolate grew optimally at pH 7.0 and 30 °C in the presence of 2 % (w/v) NaCl. The most closely related strains...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000524
更新日期:2015-11-01 00:00:00
abstract::A collection of 106 Photobacterium damselae subsp. piscicida strains and 19 Photobacterium damselae subsp. damselae strains, including reference and type strains, were genetically characterized using AFLP. The total genomic DNA of each bacterial strain was digested using restriction endonucleases HindIII and TaqI. Usi...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-50-3-1013
更新日期:2000-05-01 00:00:00
abstract::In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora longicarinata and Sophora microphylla root nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, and the strains from S. longicarinata w...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000430
更新日期:2015-10-01 00:00:00
abstract::A Gram-staining-negative, rod-shaped and non-motile bacterium, designated CL-SS4T, was isolated from crude-oil-contaminated seawater from the west coast of Korea and was investigated by means of a polyphasic taxonomic approach. Strain CL-SS4T grew optimally at 25-30 degrees C, at pH 6.8-7.7 and in the presence of 2-3%...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007708-0
更新日期:2009-08-01 00:00:00