Presence of Cryptosporidium parvum and Giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system.

Abstract:

BACKGROUND:Access to clean and safe drinking water that is free from pathogenic protozoan parasites, especially Cryptosporidium parvum and Giardia lamblia that cause gastrointestinal illness in humans, is still an issue in Southeast Asia (SEA). This study is the first attempt to detect the aforementioned protozoan parasites in water samples from countries in SEA, using real-time polymerase chain reaction (qPCR) assays. METHODS:A total of 221 water samples of 10 l each were collected between April and October 2013 from Malaysia (53), Thailand (120), the Philippines (33), and Vietnam (15). A physicochemical analysis was conducted. The water samples were processed in accordance with the US Environmental Protection Agency's methods 1622/1623.1, microscopically observed and subsequently screened using qPCR assays. RESULTS:Cryptosporidium oocysts were detected in treated water samples from the Philippines (1/10), with a concentration of 0.06 ± 0.19 oocyst/L, and untreated water samples from Thailand (25/93), Malaysia (17/44), and the Philippines (11/23), with concentrations ranging from 0.13 ± 0.18 to 0.57 ± 1.41 oocyst/L. Giardia cysts were found in treated water samples from the Philippines (1/10), with a concentration of 0.02 ± 0.06 cyst/L, and in untreated water samples from Thailand (20/93), Vietnam (5/10), Malaysia (22/44), and the Philippines (16/23), with concentrations ranging from 0.12 ± 0.3 to 8.90 ± 19.65 cyst/L. The pathogens C. parvum and G. lamblia were detected using using qPCR assays by targeting the 138-bp fragment and the small subunit gene, respectively. C. parvum was detected in untreated water samples from the Philippines (1/23) and Malaysia (2/44), whilst, G. lamblia detected was detected in treated water samples from the Philippines (1/10) and in untreated water samples from Thailand (21/93), Malaysia (12/44), and the Philippines (17/23). Nitrate concentration was found to have a high positive correlation with (oo)cyst (0.993). CONCLUSION:The presence of (oo)cysts in the water samples means that there is potential risk for zoonotic disease transmission in the studied countries. Detection using qPCR is feasible for quantifying both pathogenic C. parvum and G. lamblia in large water samples.

journal_name

Infect Dis Poverty

authors

Kumar T,Abd Majid MA,Onichandran S,Jaturas N,Andiappan H,Salibay CC,Tabo HA,Tabo N,Dungca JZ,Tangpong J,Phiriyasamith S,Yuttayong B,Polseela R,Do BN,Sawangjaroen N,Tan TC,Lim YA,Nissapatorn V

doi

10.1186/s40249-016-0095-z

subject

Has Abstract

pub_date

2016-01-13 00:00:00

pages

3

eissn

2095-5162

issn

2049-9957

pii

10.1186/s40249-016-0095-z

journal_volume

5

pub_type

杂志文章
  • Malaria over-diagnosis in Cameroon: diagnostic accuracy of Fluorescence and Staining Technologies (FAST) Malaria Stain and LED microscopy versus Giemsa and bright field microscopy validated by polymerase chain reaction.

    abstract:BACKGROUND:Malaria is a major world health issue and its continued burden is due, in part, to difficulties in the diagnosis of the illness. The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests (RDT) for all cases of suspected malaria. In regions where...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-017-0251-0

    authors: Parsel SM,Gustafson SA,Friedlander E,Shnyra AA,Adegbulu AJ,Liu Y,Parrish NM,Jamal SA,Lofthus E,Ayuk L,Awasom C,Henry CJ,McArthur CP

    更新日期:2017-04-04 00:00:00

  • HIV care-seeking behaviour after HIV self-testing among men who have sex with men in Beijing, China: a cross-sectional study.

    abstract:BACKGROUND:Men who have sex with men (MSM) has become the group with the fastest growing HIV epidemic in China. Since many Chinese MSM are conducting HIV self-testing, we aimed to determine the rate of HIV care seeking after self-testing, examine characteristics of "seekers" compared to "non-seekers," and explore facto...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-017-0326-y

    authors: Ren XL,Wu ZY,Mi GD,McGoogan JM,Rou KM,Zhao Y,Zhang N

    更新日期:2017-06-28 00:00:00

  • Cross-strait parasitological research priorities arrived at by historical tracking and advanced dialogue.

    abstract::To further enhance dialogue and promote cross-strait cooperation in the prevention and control of parasitic diseases, this paper reviewed the progress and current challenges in the cross-strait control and research of parasitic infections, based on three cross-strait meetings on parasitological research in the last de...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/2049-9957-3-40

    authors: Shin JW,Chen JX,Zhang DH,Lin WC,Shen B,Ji MJ

    更新日期:2014-12-01 00:00:00

  • Malaria epidemiology in Kobeni department, southeastern Mauritania from 2015 to 2017.

    abstract:BACKGROUND:Plasmodium falciparum malaria is endemic in the southern sahelian zone of Mauritania where intense internal and trans-border human and livestock movement occurs. The risk of importation and spread of drug-resistant parasites need to be regularly assessed in this region. The objective of the study was to asse...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-020-0634-5

    authors: Diallo SM,Bogreau H,Papa Mze N,Ould Ahmedou Salem MS,Ould Khairy ML,Parola P,Basco L,Ould Mohamed Salem Boukhary A

    更新日期:2020-02-12 00:00:00

  • Temporal trends in areas at risk for concomitant tuberculosis in a hyperendemic municipality in the Amazon region of Brazil.

    abstract:BACKGROUND:Although preventable and curable, tuberculosis (TB) still occurs in poor or developing countries, mainly in metropolitan regions of larger cities. The disease is a serious public health problem, and is directly linked to social issues. We analyzed temporal trend variations in areas at risk for concomitant TB...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-020-00732-0

    authors: Bruce ATI,Berra TZ,Dos Santos FL,Alves YM,Souza LLL,Ramos ACV,Arroyo LH,de Almeida Crispim J,Pinto IC,Palha PF,Monroe AA,Yamamura M,Fiorati RC,Moncaio ACS,de Oliveira Gomes DM,Arcêncio RA

    更新日期:2020-08-10 00:00:00

  • Parasitology should not be abandoned: data from outpatient parasitological testing in Guangdong, China.

    abstract::Over the past six decades, the Chinese government made parasitoses with a high disease burden, including soil-transmitted nematode infections, malaria, leishmaniasis, filariasis, and schistosomiasis, a public health priority because they were seen to be crucial impediments to the development of rural areas. As a resul...

    journal_title:Infectious diseases of poverty

    pub_type: 信件

    doi:10.1186/s40249-017-0332-0

    authors: Song LG,Zheng XY,Lin DT,Wang GX,Wu ZD

    更新日期:2017-09-04 00:00:00

  • Declining responsiveness of childhood Plasmodium falciparum infections to artemisinin-based combination treatments ten years following deployment as first-line antimalarials in Nigeria.

    abstract:BACKGROUND:The development and spread of artemisinin-resistant Plasmodium falciparum malaria in Greater Mekong Subregion has created impetus for continuing global monitoring of efficacy of artemisinin-based combination therapies (ACTs). This post analyses is aimed to evaluate changes in early treatment response markers...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-019-0577-x

    authors: Sowunmi A,Ntadom G,Akano K,Ibironke FO,Ayede AI,Agomo C,Folarin OA,Gbotosho GO,Happi C,Oguche S,Okafor HU,Meremikwu M,Agomo P,Ogala W,Watila I,Mokuolu O,Finomo F,Ebenebe JC,Jiya N,Ambe J,Wammanda R,Emechebe G,

    更新日期:2019-08-06 00:00:00

  • Socio-economic determinants for malaria transmission risk in an endemic primary health centre in Assam, India.

    abstract:BACKGROUND:Malaria is a major cause of morbidity and mortality in Northeast India. As there is limited information available on the potential influence of socio-economic variables on malaria risk, the present study was conducted to assess the influence of demographic factors, the socio-economic status, and knowledge, a...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/2049-9957-3-19

    authors: Yadav K,Dhiman S,Rabha B,Saikia P,Veer V

    更新日期:2014-06-24 00:00:00

  • Effects of financial incentives for treatment supporters on tuberculosis treatment outcomes in Swaziland: a pragmatic interventional study.

    abstract:BACKGROUND:Swaziland has the highest national incidence of tuberculosis (TB) in the world, with treatment success rates well below the 85 % international target. Treatment support as part of comprehensive TB services is a core component of the Stop TB Strategy. This study investigated the effects of financial incentive...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-015-0059-8

    authors: Kliner M,Canaan M,Ndwandwe SZ,Busulwa F,Welfare W,Richardson M,Walley J,Wright J

    更新日期:2015-06-05 00:00:00

  • Evaluation of malaria surveillance system in Kano State, Nigeria, 2013-2016.

    abstract:BACKGROUND:Malaria surveillance system strengthening is essential in the progress towards malaria elimination. In Nigeria, more attention is being given to this recently as the country is striving towards achieving elimination. However, the surveillance system performance is fraught with challenges including poor data ...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-020-0629-2

    authors: Visa TI,Ajumobi O,Bamgboye E,Ajayi I,Nguku P

    更新日期:2020-02-10 00:00:00

  • Risk assessment of malaria transmission at the border area of China and Myanmar.

    abstract:BACKGROUND:In order to achieve the goal of malaria elimination, the Chinese government launched the National Malaria Elimination Programme in 2010. However, as a result of increasing cross-border population movements, the risk of imported malaria cases still exists at the border areas of China, resulting in a potential...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-017-0322-2

    authors: Shi B,Zheng J,Qiu H,Yang GJ,Xia S,Zhou XN

    更新日期:2017-07-06 00:00:00

  • New focus of Kyasanur Forest disease virus activity in a tribal area in Kerala, India, 2014.

    abstract:BACKGROUND:Kyasanur Forest disease (KFD) is a febrile illness characterized by hemorrhages, and is reported endemic in the Shimoga district in Karnataka state, India. It is caused by the KFD virus (KFDV) of the family Flaviviridae, and is transmitted to monkeys and humans by Haemaphysalis ticks. FINDINGS:We investigat...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-015-0044-2

    authors: Tandale BV,Balakrishnan A,Yadav PD,Marja N,Mourya DT

    更新日期:2015-03-05 00:00:00

  • Risk factors affecting the mortality of HIV-infected patients with pulmonary tuberculosis in the cART era: a retrospective cohort study in China.

    abstract:BACKGROUND:Tuberculosis infection still places a great burden on HIV-infected individuals in China and other developing countries. Knowledge of the survival of HIV-infected patients with pulmonary tuberculosis (PTB) would provide important insights for the clinical management of this population, which remains to be wel...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-018-0405-8

    authors: Ji YJ,Liang PP,Shen JY,Sun JJ,Yang JY,Chen J,Qi TK,Wang ZY,Song W,Tang Y,Liu L,Zhang RF,Shen YZ,Lu HZ

    更新日期:2018-03-24 00:00:00

  • Selecting accurate post-elimination monitoring tools to prevent reemergence of urogenital schistosomiasis in Morocco: a pilot study.

    abstract:BACKGROUND:After alleged stop of transmission of schistosomiasis and further down the line in post elimination settings, sensitive tools are required to monitor infection status to prevent potential re-emergence. In Rahala, where transmission cycle of Schistosoma haematobium is interrupted since 2004 but where 30% of s...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-017-0289-z

    authors: Balahbib A,Amarir F,Corstjens PL,de Dood CJ,van Dam GJ,Hajli A,Belhaddad M,El Mansouri B,Sadak A,Rhajaoui M,Adlaoui EB

    更新日期:2017-04-06 00:00:00

  • Hepatitis B virus infection in undocumented immigrants and refugees in Southern Italy: demographic, virological, and clinical features.

    abstract:BACKGROUND:The data on hepatitis b virus (HBV) infection in immigrants population are scanty. The porpoise of this study was to define the demographic, virological, and clinical characteristics of subjects infected with HBV chronic infection in a cohort of immigrants living in Naples, Italy. METHODS:A screening for HB...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章,多中心研究

    doi:10.1186/s40249-016-0228-4

    authors: Coppola N,Alessio L,Gualdieri L,Pisaturo M,Sagnelli C,Minichini C,Di Caprio G,Starace M,Onorato L,Signoriello G,Macera M,Angelillo IF,Pasquale G,Sagnelli E

    更新日期:2017-02-09 00:00:00

  • An integrated environmental improvement of marshlands: impact on control and elimination of schistosomiasis in marshland regions along the Yangtze River, China.

    abstract:BACKGROUND:Schistosomiasis is a global snail-transmitted infectious disease of poverty. Transmission control had been achieved in China in 2015 after the control efforts for over 60 years. Currently, the remaining core regions endemic for Schistosoma japonicum are mainly located in the marshland and lake regions along ...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-017-0287-1

    authors: Sun LP,Wang W,Zuo YP,Zhang ZQ,Hong QB,Yang GJ,Zhu HR,Liang YS,Yang HT

    更新日期:2017-03-22 00:00:00

  • Integrating innovations: a qualitative analysis of referral non-completion among rapid diagnostic test-positive patients in Uganda's human African trypanosomiasis elimination programme.

    abstract:BACKGROUND:The recent development of rapid diagnostic tests (RDTs) for human African trypanosomiasis (HAT) enables elimination programmes to decentralise serological screening services to frontline health facilities. However, patients must still undertake multiple onwards referral steps to either be confirmed or discou...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-018-0472-x

    authors: Lee SJ,Palmer JJ

    更新日期:2018-08-18 00:00:00

  • Neglected tropical diseases and global burden of disease in China.

    abstract::Recently, the mortality of 240 causes in China including a subnational analysis during 1990-2013 was published in The Lancet. This comprehensive analysis will undoubtedly impact policymaking regarding public health in China. However, it is unfavourable in some degree to neglected tropical diseases, which is the subjec...

    journal_title:Infectious diseases of poverty

    pub_type: 信件

    doi:10.1186/s40249-017-0237-y

    authors: Qian MB

    更新日期:2017-02-03 00:00:00

  • Massive migration promotes the early spread of COVID-19 in China: a study based on a scale-free network.

    abstract:BACKGROUND:The coronavirus disease 2019 (COVID-19) epidemic met coincidentally with massive migration before Lunar New Year in China in early 2020. This study is to investigate the relationship between the massive migration and the coronavirus disease 2019 (COVID-19) epidemic in China. METHODS:The epidemic data betwee...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-020-00722-2

    authors: Song WY,Zang P,Ding ZX,Fang XY,Zhu LG,Zhu Y,Bao CJ,Chen F,Wu M,Peng ZH

    更新日期:2020-08-10 00:00:00

  • Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis.

    abstract:BACKGROUND:Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are highly endemic in Xiji County of Ningxia Hui Autonomous Region (NHAR) in China where the control campaign based on dog de-worming with praziquantel has been undertaken over preceding decades. This study is to determine the current prevalence of ...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-018-0458-8

    authors: Liu CN,Xu YY,Cadavid-Restrepo AM,Lou ZZ,Yan HB,Li L,Fu BQ,Gray DJ,Clements AA,Barnes TS,Williams GM,Jia WZ,McManus DP,Yang YR

    更新日期:2018-08-09 00:00:00

  • Why more research needs to be done on echinococcosis in Pakistan.

    abstract:BACKGROUND:Echinococcosis has a worldwide geographical distribution with endemic foci on every inhabited continent. Due to the frequent outbreaks in different parts of Pakistan in the recent past, echinococcosis is being described as a neglected tropical disease and is considered one of the most neglected parasitic dis...

    journal_title:Infectious diseases of poverty

    pub_type: 信件

    doi:10.1186/s40249-017-0309-z

    authors: Ahmed H,Ali S,Afzal MS,Khan AA,Raza H,Shah ZH,Simsek S

    更新日期:2017-07-03 00:00:00

  • Study roadmap for high-throughput development of easy to use and affordable biomarkers as diagnostics for tropical diseases: a focus on malaria and schistosomiasis.

    abstract:BACKGROUND:Interventions are currently being used against 'infectious diseases of poverty', which remain highly debilitating and deadly in most endemic countries, especially malaria, schistosomiasis, echinococcosis and African sleeping sickness. However, major limitations of current 'traditional' methods for diagnosis ...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-017-0344-9

    authors: Kassegne K,Zhang T,Chen SB,Xu B,Dang ZS,Deng WP,Abe EM,Shen HM,Hu W,Guyo TG,Nwaka S,Chen JH,Zhou XN

    更新日期:2017-10-02 00:00:00

  • Knowledge of, attitudes towards, and practice relating to schistosomiasis in two subtypes of a mountainous region of the People's Republic of China.

    abstract:BACKGROUND:Schistosomiasis japonica is still endemic in the People's Republic of China (P.R. China) in five provinces of lake and marshland regions and in two provinces of mountainous regions. Studies elucidated that individual and community perception, attitudes towards schistosomiasis, and hygiene behaviors were cruc...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/2049-9957-3-16

    authors: Liu L,Yang GJ,Zhu HR,Yang K,Ai L

    更新日期:2014-05-07 00:00:00

  • Are tuberculosis patients adherent to prescribed treatments in China? Results of a prospective cohort study.

    abstract:BACKGROUND:Tuberculosis (TB) patients face numerous difficulties adhering to the long-term, rigorous TB treatment regimen. Findings on TB patients' treatment adherence vary across existing literature and official reports. The present study attempted to determine the actual treatment adherence of new TB patients and to ...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-016-0134-9

    authors: Lei X,Huang K,Liu Q,Jie YF,Tang SL

    更新日期:2016-05-05 00:00:00

  • Geographic distribution of echinococcosis in Tibetan region of Sichuan Province, China.

    abstract:BACKGROUND:Echinococcosis is a parasitic zoonosis caused by Echinococcus larvae parasitism causing high mortality. The Tibetan Region of Sichuan Province is a high prevalence area for echinococcosis in China. Understanding the geographic distribution pattern is necessary for precise control and prevention. In this stud...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-018-0486-4

    authors: Liu L,Guo B,Li W,Zhong B,Yang W,Li SC,Wang Q,Zhao X,Xu KJ,Qin SC,Huang Y,Yu WJ,He W,Liao S,Wang Q

    更新日期:2018-11-02 00:00:00

  • Burden, risk assessment, surveillance and management of SARS-CoV-2 infection in health workers: a scoping review.

    abstract:BACKGROUND:Health workers (HWs) are at increased risk for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection and a possible source of nosocomial transmission clusters. Despite the increased risk, the best surveillance strategy and management of exposed HWs are not yet well known. The aim of this rev...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章,评审

    doi:10.1186/s40249-020-00756-6

    authors: Calò F,Russo A,Camaioni C,De Pascalis S,Coppola N

    更新日期:2020-10-07 00:00:00

  • Interventions for vector-borne diseases focused on housing and hygiene in urban areas: a scoping review.

    abstract:BACKGROUND:Over half the world's human populations are currently at risk from vector-borne diseases (VBDs), and the heaviest burden is borne by the world's poorest people, communities, and countries. The aim of this study was to conduct a review on VBD interventions relevant to housing and hygiene (including sanitation...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章,评审

    doi:10.1186/s40249-018-0477-5

    authors: Degroote S,Zinszer K,Ridde V

    更新日期:2018-09-03 00:00:00

  • Seroprevalence of Toxoplasma gondii infection among patients with hand, foot and mouth disease in Henan, China: a hospital-based study.

    abstract:BACKGROUND:The prevalence of infection with Toxoplasma gondii (T. gondii) in humans has been increasing in China due to the growing number of cats in the country. Hand, foot and mouth disease (HFMD) is a serious public health issue in China and still one of the leading causes of child mortality. However, little is know...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-015-0088-3

    authors: Wang S,Lan C,Zhang L,Zhang H,Yao Z,Wang D,Ma J,Deng J,Liu S

    更新日期:2015-12-10 00:00:00

  • Malaria endemicity and co-infection with tissue-dwelling parasites in Sub-Saharan Africa: a review.

    abstract::Mechanisms and outcomes of host-parasite interactions during malaria co-infections with gastrointestinal helminths are reasonably understood. In contrast, very little is known about such mechanisms in cases of malaria co-infections with tissue-dwelling parasites. This is lack of knowledge is exacerbated by misdiagnosi...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/s40249-015-0070-0

    authors: Onkoba NW,Chimbari MJ,Mukaratirwa S

    更新日期:2015-08-29 00:00:00

  • Global output of research on epidermal parasitic skin diseases from 1967 to 2017.

    abstract:BACKGROUND:Epidermal parasitic skin diseases (EPSD) occur in most countries and cause a considerable health and economic burden, particularly in the tropics and subtropics. The aim of this study was to assess and analyse peer-reviewed literature on EPSD in humans. The results of this study serve as an indicator of the ...

    journal_title:Infectious diseases of poverty

    pub_type: 杂志文章

    doi:10.1186/s40249-018-0456-x

    authors: Sweileh WM

    更新日期:2018-08-06 00:00:00