当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Synergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype.

Synergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype.

Abstract：

:Hypoxia is a pathalogical condition in which tissues are deprived of adequate oxygen supply. The hypoxia effect on tumors has a critically important role on maintenance of cancer stem cell phenotype. The aim of this study is to investigate the effects of hypoxia on cancer stem cells on three dimensional (3D) in vitro culture models. Osteosarcoma stem cells characterized by CD133 surface protein were isolated from osteosarcoma cell line (SaOS-2) by magnetic-activated cell sorting (MACS) technique. Isolated CD133(+) and CD133(-) cells were cultivated under hypoxic (1% O2) and normoxic conditions (21% O2) for 3 days. For the 3D model, bacterial cellulose scaffold was used as the culture substrate. 3D morphologies of cells were examined by scanning electron microscopy (SEM); RT-PCR and immunocytochemistry staining were used to demonstrate conservation of the cancer stem cell phenotype in 3D environment under hypoxic conditions. Cell viability was shown by MTT assay on 3. and 7. culture days. This study is seen as an introduction to develop a 3D hypoxic cancer stem cell based tumor model to study CSC behavior and tumor genesis in vitro.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Gorgun C,Ozturk S,Gokalp S,Vatansever S,Gurhan SI,Urkmez AS

doi

10.1016/j.ijbiomac.2015.12.053

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

20-6

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(15)30240-3

journal_volume

90

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Antioxidant activities of sulfated pumpkin polysaccharides.

    abstract：:Pumpkin polysaccharide was extracted by hot water extraction method. It was modified with chlorosulfonic acid-pyridine to obtain sulfated pumpkin polysaccharides (SP1, SP2) with different degrees of substitution, which was 0.35 and 0.65, respectively. The total sugar contents of pumpkin polysaccharide and its sulfated...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.261

    authors： Chen L,Huang G

    更新日期：2019-04-01 00:00:00

  • Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus.

    abstract：:Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent st...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.167

    authors： Sun YG,Li R,Jiang L,Qiao S,Zhi Y,Chen XX,Xie S,Wu J,Li X,Deng R,Zhang G

    更新日期：2018-10-01 00:00:00

  • Folding thermodynamics of c-Myb DNA-binding domain in correlation with its α-helical contents.

    abstract：:The conformational and thermal stabilities of the minimum functional unit for c-Myb DNA-binding domain, tandem repeat 2 and 3 (R2R3), were analyzed under different pH conditions, ranging from 4.0 to 7.5, using circular dichroism and differential scanning calorimetry. Secondary structure analysis showed that the soluti...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.10.035

    authors： Inaba S,Fukada H,Oda M

    更新日期：2016-01-01 00:00:00

  • Use of Plantago major seed mucilage as a novel edible coating incorporated with Anethum graveolens essential oil on shelf life extension of beef in refrigerated storage.

    abstract：:In this study, Plantago major seed mucilage (PMSM) was extracted from whole seeds using hot-water extraction (HWE). The dill (D) essential oil components were identified through gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) and its antioxidant properties were examined through the methods of ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.055

    authors： Behbahani BA,Shahidi F,Yazdi FT,Mortazavi SA,Mohebbi M

    更新日期：2017-01-01 00:00:00

  • Chelate-soluble pectin fraction from papaya pulp interacts with galectin-3 and inhibits colon cancer cell proliferation.

    abstract：:Colorectal cancer has an overexpression of galectin-3 that is related to cancer progression. A decreased risk of colon cancer can be related to consumption of dietary fibers, but the entire mechanism by which this protection occurs remains unclear. Pectin is a type of dietary fiber that possesses β-galactosides and ca...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.191

    authors： Prado SBRD,Santos GRC,Mourão PAS,Fabi JP

    更新日期：2019-04-01 00:00:00

  • Identification and characterization of Pseudomonas aeruginosa derived bacteriocin for industrial applications.

    abstract：:Drug resistance has become a major threat due to the frequent use of commercial antibiotics and there is an urgent need to combat this problem. Having this in mind, the present research was aimed at developing a novel P. aeruginosa puBac bacteriocin molecule. The bacteriocin was purified by ammonium sulfate precipitat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.10.126

    authors： Lakshmanan R,Kalaimurugan D,Sivasankar P,Arokiyaraj S,Venkatesan S

    更新日期：2020-12-15 00:00:00

  • Formation, derivatization and applications of bacterial cellulose.

    abstract：:Acetobacter xylinum produces highly crystalline cellulose extracellularly using glucose as a carbon source. The polymer formed is free of other biogenic compounds, separable in a simple way and characterized by its high water-absorption capacity. Stepwise solvent exchange from water to unpolar solvents leads to a dras...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(94)90067-1

    authors： Geyer U,Heinze T,Stein A,Klemm D,Marsch S,Schumann D,Schmauder HP

    更新日期：1994-12-01 00:00:00

  • Isolation, purification and structural investigation of a water-soluble polysaccharide from Solanum lyratum Thunb.

    abstract：:Polysaccharides, extracted from the herbs of Chinese natural Solanum lyratum Thunb (SLT), which is a traditional Chinese medicine with hot water and 0.04 M sodium hydroxide successively, were fractionated and purified by ion-exchange and gel-filtration chromatography. According to methylation, periodate oxidation, NMR...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2005.06.007

    authors： Yalin W,Yuanjiang P,Cuirong S

    更新日期：2005-09-15 00:00:00

  • The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.

    abstract：:It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels. In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.208

    authors： Kong W,Gao Y,Liu Q,Dong L,Guo L,Fan H,Fan Y,Zhang X

    更新日期：2020-10-01 00:00:00

  • Structuring oil-in-water emulsion by forming egg yolk/alginate complexes: Their potential application in fabricating low-fat mayonnaise-like emulsion gels and redispersible solid emulsions.

    abstract：:Overconsumption of fat-rich foods has caused increasing incidences of human chronic diseases. Thus, it is promising to fabricate reduced-fat foods, but the reduction in fat content often causes undesired textural changes in foods. In this study, we showed a facile method to structure oil-in-water emulsions via the ele...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.106

    authors： Yang X,Li A,Yu W,Li X,Sun L,Xue J,Guo Y

    更新日期：2020-03-15 00:00:00

  • Simultaneous degumming and production of a natural gum from Crotalaria juncea seeds: Physicochemical and rheological characterization.

    abstract：:The oil extracted from Crotalaria juncea (Sunn-hemp) contains 70% of gum. Several methods of degumming are attempted in order to maximize the yield of gum. During appropriate water induced degumming, about 95-98% of phosphatides are separated. The maximum oil yield for two types of degummimg processes are 0.59% and 0....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.12.146

    authors： Sadhukhan S,Bhattacharjee A,Sarkar U,Baidya PK,Baksi S

    更新日期：2018-05-01 00:00:00

  • Observation of in vitro cellulose synthesis by bacterial cellulose synthase with time-resolved small angle X-ray scattering.

    abstract：:Cellulose synthase is the enzyme that produces cellulose in the living organisms like plant, and has two functions: polymerizing glucose residues (polymerization) and assembling these polymerized molecules into a crystalline microfibril with a "cellulose I" crystallographic structure (crystallization). Many studies, h...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.167

    authors： Tajima H,Penttilä PA,Imai T,Yamamoto K,Yuguchi Y

    更新日期：2019-06-01 00:00:00

  • Thermo-sensitive chitosan copolymer-gold hybrid nanoparticles as a nanocarrier for delivery of erlotinib.

    abstract：:Here, using (poly(N-isopropylacrylamide)-co-oleic acid)-g-chitosan ((PNIPAAm-co-OA)-g-CS), CS copolymer-gold hybrid nanoparticles (CGH NPs) were synthesized by autoreduction of auric cations (HAuCl4) in aqueous solution in the absence of any other reducing agent. The engineered thermo-sensitive CS copolymer with free ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.020

    authors： Fathi M,Sahandi Zangabad P,Barar J,Aghanejad A,Erfan-Niya H,Omidi Y

    更新日期：2018-01-01 00:00:00

  • The interaction of amino acids, peptides, and proteins with DNA.

    abstract：:Amino acids that carry charges on their side groups can bind to double stranded DNA (dsDNA) and change the strength of the double helix. Measurement of the DNA melting temperature (Tm) confirmed that acidic amino acids (Glu, Asp) weaken the H-bonds between DNA strands, whereas basic amino acids (Arg, Lys) strengthen t...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.054

    authors： Solovyev AY,Tarnovskaya SI,Chernova IA,Shataeva LK,Skorik YA

    更新日期：2015-01-01 00:00:00

  • Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells.

    abstract：:In this study, an oxidative stress model was first developed in a mouse macrophage cell line (RAW264.7 cells) by infecting the cells with porcine circovirus type 2 (PCV2). The regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress was investigated. The results showed that after ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.09.026

    authors： Su ZJ,Wei YY,Yin D,Shuai XH,Zeng Y,Hu TJ

    更新日期：2013-11-01 00:00:00

  • Synthesis and characterization of schizophyllan nanogels via inverse emulsion using biobased materials.

    abstract：:Oxidized sucrose cross-linked Schizophyllan nanogel was successfully synthesized via inverse emulsion method for the first time. The synthesis process was conducted in the absence of both toxic cross-linker and organic solvent. The nanogel crosslinking network was prepared using fractionated coconut oil as the continu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.119

    authors： Mousaviasl S,Saleh T,Shojaosadati SA,Boddohi S

    更新日期：2018-12-01 00:00:00

  • Evaluation of BP-M-CPF4 polyhydroxyalkanoate (PHA) synthase on the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil using Cupriavidus necator transformants.

    abstract：:Among the various types of polyhydroxyalkanoate (PHA), poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] has a high potential to serve as commercial bioplastic due to its striking resemblance to petroleum-based plastics. In this study, five different genotypes of Cupriavidusnecator transformants h...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.064

    authors： Tan HT,Chek MF,Lakshmanan M,Foong CP,Hakoshima T,Sudesh K

    更新日期：2020-09-15 00:00:00

  • In situ decorated Au NPs on pectin-modified Fe3O4 NPs as a novel magnetic nanocomposite (Fe3O4/Pectin/Au) for catalytic reduction of nitroarenes and investigation of its anti-human lung cancer activities.

    abstract：:In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future catalysts. This has encouraged us to design and synthesis of a novel Au NPs immobilized pectin modified magnetic nanoparticles (Fe3O4/Pectin/Au). It was meticulously characterized using advanced a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.102

    authors： Li Y,Li N,Jiang W,Ma G,Zangeneh MM

    更新日期：2020-11-15 00:00:00

  • Preparation of chitosan derivative with polyethylene glycol side chains for porous structure without specific processing technique.

    abstract：:The graft copolymer, chitosan-g-polyethylene glycol (PEG), was prepared through graft polymerization of PEG chains to chitosan due to the esterification reaction between PEG and 6-O-succinate-N-phthaloyl-chitosan (PHCSSA). The graft copolymer with porous structure was observed from scanning electron micrographs. It is...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2006.02.008

    authors： Huang M,Liu L,Zhang G,Yuan G,Fang Y

    更新日期：2006-05-30 00:00:00

  • Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts.

    abstract：:A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities were 60%, 58%, 41% and 14%, respectively, very likely due to casein d...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.140

    authors： Siar EH,Morellon-Sterling R,Zidoune MN,Fernandez-Lafuente R

    更新日期：2020-02-01 00:00:00

  • Polyethyleneimine-protein interactions and implications on protein stability.

    abstract：:Protein stability assessment of seven model proteins in the presence of low molecular weight polyethyleneimine (PEI, MW 2000 Da) was performed. Thermodynamic stability, monitored by circular dichroism (CD) spectroscopy, showed that the polymer did not have a major effect on the melting temperature (T(m)) of the basic ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.04.003

    authors： Mazzaferro L,Breccia JD,Andersson MM,Hitzmann B,Hatti-Kaul R

    更新日期：2010-07-01 00:00:00

  • Sugar compositions, α-glucosidase inhibitory and amylase inhibitory activities of polysaccharides from leaves and flowers of Camellia sinensis obtained by different extraction methods.

    abstract：:The sugar compositions, α-glucosidase inhibitory and α-amylase inhibitory activities of polysaccharides from leaves and flowers of green tea (Camellia sinensis) obtained by hot water extraction (HWE), boiled water extraction (BWE) and enzymatic extraction (EE) were investigated. The yields, sugar contents and monosacc...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.07.007

    authors： Wang Y,Yang Z,Wei X

    更新日期：2010-11-01 00:00:00

  • Effect of o-acyl substituents on the functional behaviour of Rhizobium meliloti succinoglycan.

    abstract：:The effects of selective removal of acetyl or succinyl substituents on the functionality of succinoglycan polysaccharide have been studied by comparing the behaviour of the polysaccharides isolated from native Rhizobium meliloti strain Rm1021, and genetically modified R. meliloti species. Removal of the succinyl group...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(96)01140-3

    authors： Ridout MJ,Brownsey GJ,York GM,Walker GC,Morris VJ

    更新日期：1997-02-01 00:00:00

  • Effects of Glycyrrhiza glabra polysaccharides on immune and antioxidant activities in high-fat mice.

    abstract：:The purpose of this study was to investigate the immune and antioxidant activities of Glycyrrhiza glabra polysaccharides (GGP) in rats fed high-fat diet. The experiment was performed on four groups of growing Kunming mice. The results of the experiment showed a statistically significant decrease in serum antioxidant e...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2009.04.001

    authors： Hong YK,Wu HT,Ma T,Liu WJ,He XJ

    更新日期：2009-07-01 00:00:00

  • Poly (A) binding protein enhances the binding affinity of potyvirus VPg to eukaryotic initiation factor eIF4F and activates in vitro translation.

    abstract：:Viral protein linked to the genome (VPg) of turnip mosaic virus (TuMV) interacts with eIF4F and plays an important role in cap-independent initiation of protein synthesis. In order to understand the importance of PABP on the interaction of eIF4F with VPg, we report that PABP enhanced the binding affinity ~3- and 4-fol...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.135

    authors： Khan MA,Goss DJ

    更新日期：2019-01-01 00:00:00

  • Bio-based Preservative using Methyl-β-cyclodextrin-Essential Oil Complexes for Wood Protection.

    abstract：:Developing natural preservatives for wood protection is of great interest in sustainable construction and green building industries. This study was designed to determine the feasibility of using methyl-β-cyclodextrin-essential oils (MβCD-EOs) complexes as potential bio-based preservatives for wood protection. Four ess...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.056

    authors： Cai L,Lim H,Nicholas DD,Kim Y

    更新日期：2020-03-15 00:00:00

  • Bufadienolides from Kalanchoe daigremontiana modulate the enzymatic activity of plasmin - In vitro and in silico analyses.

    abstract：:Plasmin (EC 3.4.21.7) is a key enzyme of the fibrinolytic system, responsible for the degradation of fibrin clot and maintaining blood fluidity. Hence, alterations of the fibrinolytic capacity of blood plasma may contribute to thrombotic or bleeding complications. The aim of this study was to determine effects of a bu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.143

    authors： Kolodziejczyk-Czepas J,Pasiński B,Ponczek MB,Moniuszko-Szajwaj B,Kowalczyk M,Pecio Ł,Nowak P,Stochmal A

    更新日期：2018-12-01 00:00:00

  • Dual-layered pH-sensitive alginate/chitosan/kappa-carrageenan microbeads for colon-targeted release of 5-fluorouracil.

    abstract：:A new pH-sensitive drug carrier based on alginate (Alg), chitosan (Cs), and kappa-carrageenan (kC) marine biopolymers was developed, comprising a dual-layered polyelectrolyte complexes microbead structure. Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and scanning electron microsco...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.225

    authors： Sun X,Liu C,Omer AM,Yang LY,Ouyang XK

    更新日期：2019-07-01 00:00:00

  • Iminodiacetic acid modified kenaf fiber for waste water treatment.

    abstract：:In the present study, iminodiacetic acid (IDA)-modified kenaf fiber, K-IDA formed by the chemical modification of plant kenaf biomass was tested for its efficacy as a sorbent material towards the purification of waste water. The K-IDA fiber was first characterized by the instrumental techniques like Fourier transform ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.02.035

    authors： Razak MR,Yusof NA,Haron MJ,Ibrahim N,Mohammad F,Kamaruzaman S,Al-Lohedan HA

    更新日期：2018-06-01 00:00:00

  • Size effect-inspired fabrication of konjac glucomannan/polycaprolactone fiber films for antibacterial food packaging.

    abstract：:The exploration of new methods to produce food packaging with excellent physicochemical and antibacterial properties is of great scientific and technological interest. Here, we successfully prepare the food packaging films that are composed of konjac glucomannan (KGM), poly(ε-caprolactone) (PCL) and silver nanoparticl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.242

    authors： Lin W,Ni Y,Pang J

    更新日期：2020-04-15 00:00:00