Abstract：
:Peanut proteins conarachin II, conarachin I and arachin were found to behave as highly selective fluorescence sensors for periodate amongst a set of different anions. The interactions of the proteins with periodate were also confirmed by other spectral methods and enzyme linked immunosorbent assay (ELISA). The results indicate a selective interaction of peanut proteins with periodate amongst chloride, sulphate, iodide, phosphate, nitrate, nitrite, bromide, fluoride, persulphate, acetate, thiosulphate, arsenite, arsenate, sulphite, and iodide. Periodate sensing using different synthesized organic molecules are already reported in the literature. In this article we report the efficiency of peanut proteins as anion sensor which are bioactive and inexpensive too. The protein periodate interactions have also resulted in a simultaneous reduction in allergenicity of the peanut proteins. A change in the secondary structure of the protein was found responsible for this change which was further established with the help of circular dichroism and Raman spectroscopy.
journal_name
Food Chemjournal_title
Food chemistryauthors
Ghatak SK,Sen S,Majumdar D,Singha A,Sen Kdoi
10.1016/j.foodchem.2015.11.039subject
Has Abstractpub_date
2016-04-15 00:00:00pages
1286-91eissn
0308-8146issn
1873-7072pii
S0308-8146(15)30186-2journal_volume
197 Pt Bpub_type
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