Abstract：
:Phenolic extracts from cv Tempranillo grapes subjected to water stress and irrigation treatment, both of them with high and low crop load, were analyzed by front-face fluorescence. Excitation-emission matrices (EEMs) were analyzed by means of unsupervised parallel factor analysis (PARAFAC), PARAFAC supervised by linear discriminant analysis, and discriminant unfolded partial least-squares. All algorithms allowed to differentiate between water stress and irrigation grape samples when the fluorescence maxima region of catechin and epicatechin, and resveratrol was considered. A central composite design was employed for the calibration of catechin, epicatechin and resveratrol. Resveratrol was quantified by U-PLS in both, irrigated and water stressed samples, and levels between 3.46 ± 0.22 and 4.67 ± 0.08 μg mL-1 and 2.43 ± 0.60 and 3.03 ± 0.10 μg mL-1, respectively, were found. PARAFAC only allowed the determination of the sum of catechin plus epicatechin (R2 = 0.9397). The determination of total catechin plus epicatechin by means of PARAFAC was successfully validated by liquid chromatography.
journal_name
Food Chemjournal_title
Food chemistryauthors
Cabrera-Bañegil M,Valdés-Sánchez E,Moreno D,Airado-Rodríguez D,Durán-Merás Idoi
10.1016/j.foodchem.2018.07.071subject
Has Abstractpub_date
2019-01-01 00:00:00pages
162-172eissn
0308-8146issn
1873-7072pii
S0308-8146(18)31207-Xjournal_volume
270pub_type
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