Tsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis.

Abstract:

:Three bacterial strains, HKU51T, HKU52T and HKU53, were isolated from a conjunctival swab, corneal scraping and blood culture of three patients in Hong Kong with conjunctivitis, keratitis and catheter-related bacteraemia, respectively. Cells were Gram-stain-positive, aerobic, catalase-positive, non-sporulating and non-motile bacilli. The three strains had unique biochemical profiles that were distinguishable from those of closely related species of the genus Tsukamurella. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU52T and HKU53, and the two strains shared 99.5 % sequence identity with Tsukamurella sunchonensis JCM 15929T and Tsukamurella pseudospumae JCM 13375T; HKU51T shared 99.6 % sequence identity with Tsukamurella pulmonis CCUG 35732T. The DNA G+C contents of strains HKU51T, HKU52T and HKU53 were 70.9 ± 2.2, 71.3 ± 2.1 and 71.2 ± 2.3 mol% (mean ± sd; n = 3), respectively. DNA-DNA hybridization confirmed that the novel strains were distinct from other known species of the genus Tsukamurella ( ≤ 50.1 ± 3.7 % DNA-DNA relatedness); two of the isolates, HKU52T and HKU53, represented the same species ( ≥ 94.6 ± 5.6 % DNA-DNA relatedness), while the third isolate, HKU51T, represented another species. The novel species Tsukamurella hongkongensis sp. nov. is proposed to accommodate strains HKU52T and HKU53, with HKU52T ( = JCM 30715T = DSM 100208T) as the type strain; whilst another novel species, Tsukamurella sinensis sp. nov., is proposed to accommodate the third isolate, HKU51T ( = JCM 30714T = DSM 100207T), which is designated the type strain.

authors

Teng JLL,Tang Y,Wong SSY,Ngan AHY,Huang Y,Tsang CC,Choi GKY,Lau SKP,Woo PCY

doi

10.1099/ijsem.0.000733

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

391-397

issue

1

eissn

1466-5026

issn

1466-5034

journal_volume

66

pub_type

杂志文章
  • Bensingtonia changbaiensis sp. nov. and Bensingtonia sorbi sp. nov., novel ballistoconidium-forming yeast species from plant leaves.

    abstract::Six ballistoconidium-forming yeast strains that were isolated from plant leaves collected on Changbai Mountain, north-east China, were assigned to the genus Bensingtonia Ingold emend. Nakase & Boekhout due to the formation of asymmetrical ballistoconidia, cream-coloured colonies and Q-9 as the major ubiquinone. Two se...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02736-0

    authors: Wang QM,Bai FY,Zhao JH,Jia JH

    更新日期:2003-11-01 00:00:00

  • Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland.

    abstract::Peatlands are important sources of CH(4) emissions to the atmosphere and molecular surveys have identified a diverse, but mainly uncultured, euryarchaeal community in them. Characterization of a strain, E1-9c(T), associated with uncultured group E1, from a minerotrophic fen is reported. Cells were regular cocci, usual...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.006890-0

    authors: Cadillo-Quiroz H,Yavitt JB,Zinder SH

    更新日期:2009-05-01 00:00:00

  • Clostridium neonatale sp. nov. linked to necrotizing enterocolitis in neonates and a clarification of species assignable to the genus Clostridium (Prazmowski 1880) emend. Lawson and Rainey 2016.

    abstract::A description of an outbreak of necrotizing enterocolitis among neonates, linked to the putative novel species Clostridium neonatale and assignable to the genus Clostridium, was previously reported in brief but that name had never been validly published (Alfa et al. Clin Inf Dis 2002;35:S101-S105). Features of this ta...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002827

    authors: Bernard K,Burdz T,Wiebe D,Alfa M,Bernier AM

    更新日期:2018-08-01 00:00:00

  • Erythrobacter aquimixticola sp. nov., isolated from the junction between the ocean and a freshwater spring.

    abstract::A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated JSSK-14T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-14T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002055

    authors: Park S,Jung YT,Choi SJ,Yoon JH

    更新日期:2017-08-01 00:00:00

  • Snuella lapsa gen. nov., sp. nov., isolated from tidal flat sediment.

    abstract::A yellow-coloured, rod-shaped, Gram-reaction-negative, aerobic bacterial strain, designated JC2132(T), was isolated from a tidal flat sediment sample from Ganghwa Island, Korea. The isolate required sea salts for growth. Cells produced non-diffusible carotenoid pigments, but flexirubin-type pigments were absent. Glidi...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.027458-0

    authors: Yi H,Chun J

    更新日期:2011-10-01 00:00:00

  • Neisseria weixii sp. nov., isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae).

    abstract::Three independent isolates (10022T, 10 009 and 10011) of a novel catalase-positive, Gram-stain-negative coccus in the genus Neisseria were obtained from the rectal contents of plateau pika on the Qinghai-Tibet Plateau, PR China. Based on 16S rRNA gene sequence analysis, our newly identified organisms were most closely...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003466

    authors: Zhang G,Yang J,Lai XH,Jin D,Pu J,Bai X,Xiong Y,Ren Z,Luo X,Huang Y,Lu S,Xu J

    更新日期:2019-08-01 00:00:00

  • Genetic diversity of Agrobacterium species isolated from nodules of common bean and soybean in Brazil, Mexico, Ecuador and Mozambique, and description of the new species Agrobacterium fabacearum sp. nov.

    abstract::Agrobacterium strains are associated with soil, plants and animals, and known mainly by their pathogenicity. We studied 14 strains isolated from nodules of healthy soybean and common bean plants in Brazil, Mexico, Ecuador and Mozambique. Sequence analysis of the 16S rRNA gene positioned the strains as Agrobacterium, b...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004278

    authors: Delamuta JRM,Scherer AJ,Ribeiro RA,Hungria M

    更新日期:2020-07-01 00:00:00

  • Candida olivae sp. nov., a novel yeast species from 'Greek-style' black olive fermentation.

    abstract::Two yeast strains (FMCC Y-1(T) and FMCC Y-2) were recovered during a survey of the yeast biota associated with fermenting black olives, collected from an olive tree (Olea europaea L. cv. 'Conservolea') orchard in Central Greece. Phylogenetic analysis based on rRNA gene sequences (18S, 26S, and 5.8S-ITS) indicated that...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.012955-0

    authors: Nisiotou AA,Panagou EZ,Nychas GE

    更新日期:2010-05-01 00:00:00

  • Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring.

    abstract::A pleomorphic, extremely halophilic archaeon (strain M6(T)) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0.8-1.0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63211-0

    authors: Elshahed MS,Savage KN,Oren A,Gutierrez MC,Ventosa A,Krumholz LR

    更新日期:2004-11-01 00:00:00

  • Streptosporangium saharense sp. nov., an actinobacterium isolated from Saharan soil.

    abstract::A novel actinobacterium, designated strain SG20T, was isolated from a Saharan soil sample collected from Béni-isguen (Mzab), Ghardaïa province, southern Algeria. The micro-organism developed small roundish sporangia on aerial mycelium that were sessile or carried by very short sporangiophores. The cell-wall peptidogly...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000890

    authors: Chaabane Chaouch F,Bouras N,Mokrane S,Zitouni A,Schumann P,Spröer C,Sabaou N,Klenk HP

    更新日期:2016-03-01 00:00:00

  • Multigene characterization of a new 'Candidatus Phytoplasma rubi'-related strain associated with blackberry witches' broom.

    abstract::A new phytoplasma was identified in naturally infected blackberry plants exhibiting witches' broom symptoms in Portugal. The 16S rRNA gene sequence revealed that it is related to 'Candidatus Phytoplasma rubi' (16SrV-E ribosomal subgroup) and RFLP analysis revealed a unique profile following MseI endonuclease digestion...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000871

    authors: Fránová J,de Sousa E,Koloniuk I,Mimoso C,Matos J,Cardoso F,Contaldo N,Paltrinieri S,Bertaccini A

    更新日期:2016-03-01 00:00:00

  • Mucinivorans hirudinis gen. nov., sp. nov., an anaerobic, mucin-degrading bacterium isolated from the digestive tract of the medicinal leech Hirudo verbana.

    abstract::Three anaerobic bacterial strains were isolated from the digestive tract of the medicinal leech Hirudo verbana, using mucin as the primary carbon and energy source. These strains, designated M3(T), M4 and M6, were Gram-stain-negative, non-spore-forming and non-motile. Cells were elongated bacilli approximately 2.4 µm ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000052

    authors: Nelson MC,Bomar L,Maltz M,Graf J

    更新日期:2015-03-01 00:00:00

  • Actinokineospora spheciospongiae sp. nov., isolated from the marine sponge Spheciospongia vagabunda.

    abstract::A Gram-staining-positive, aerobic organism, isolated from the Red Sea sponge Spheciospongia vagabunda was investigated to determine its taxonomic position. On the basis of results of 16S rRNA gene sequence analysis strain EG49(T) was most closely related to Actinokineospora cibodasensis and Actinokineospora baliensis ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000031

    authors: Kämpfer P,Glaeser SP,Busse HJ,Abdelmohsen UR,Ahmed S,Hentschel U

    更新日期:2015-03-01 00:00:00

  • Complete genome sequence and cell structure of Limnochorda pilosa, a Gram-negative spore-former within the phylum Firmicutes.

    abstract::Limnochorda pilosa is a pleomorphic facultative anaerobe and the sole species in the class Limnochordia, which has tentatively been placed in the phylum Firmicutes. In the present study, the complete genome sequence of L. pilosa HC45T was obtained and analysed. The genome size was 3.82 Mbp and the DNA G+C content was ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000881

    authors: Watanabe M,Kojima H,Fukui M

    更新日期:2016-03-01 00:00:00

  • Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier's gangrene.

    abstract::A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715(T), was isolated from the blood of a patient with Fournier's gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715(T) was shown to belong to the genus Atopobium and was most closely related to...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.065243-0

    authors: Cools P,Oyaert M,Vaneechoutte M,De Laere E,Vervaeke S

    更新日期:2014-09-01 00:00:00

  • Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia, and the phylum Thermodesulfobacteria into four phyla reflecting major functional capabilities.

    abstract::The class Deltaproteobacteria comprises an ecologically and metabolically diverse group of bacteria best known for dissimilatory sulphate reduction and predatory behaviour. Although this lineage is the fourth described class of the phylum Proteobacteria, it rarely affiliates with other proteobacterial classes and is f...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004213

    authors: Waite DW,Chuvochina M,Pelikan C,Parks DH,Yilmaz P,Wagner M,Loy A,Naganuma T,Nakai R,Whitman WB,Hahn MW,Kuever J,Hugenholtz P

    更新日期:2020-11-01 00:00:00

  • Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.

    abstract::A Gram-negative, non-spore-forming, yellow-pigmented, slightly halophilic bacterial strain, SW-109(T), was isolated from a tidal flat of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. This isolate did not produce bacteriochlorophyll a and contained ubiquinone-10 as the predominant respiratory ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63522-0

    authors: Yoon JH,Kang KH,Yeo SH,Oh TK

    更新日期:2005-05-01 00:00:00

  • Nocardia exalbida sp. nov., isolated from Japanese patients with nocardiosis.

    abstract::Two bacterial strains isolated from different hospitals in Japan were subjected to a polyphasic analysis. Strains IFM 0803(T) and IFM 10383 were found to have morphological, biochemical, physiological and chemotaxonomic properties consistent with their classification in the genus Nocardia. Strains IFM 0803(T) and IFM ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63850-0

    authors: Iida S,Kageyama A,Yazawa K,Uchiyama N,Toyohara T,Chohnabayashi N,Suzuki SI,Nomura F,Kroppenstedt RM,Mikami Y

    更新日期:2006-06-01 00:00:00

  • Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum.

    abstract::In the course of isolating bacteria from infective juveniles of the entomopathogenic nematode Steinernema thermophilum Ganguly & Singh, 2000, three isolates were obtained (OP1T, OP29 and VS3). On the basis of 16S rRNA gene sequence analysis and riboprint patterns, these three strains were identical to each other but d...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63973-0

    authors: Somvanshi VS,Lang E,Sträubler B,Spröer C,Schumann P,Ganguly S,Saxena AK,Stackebrandt E

    更新日期:2006-03-01 00:00:00

  • Pigmentiphaga humi sp. nov., isolated from soil amended with humic acid.

    abstract::A slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-318T, was isolated from soil in a field located in Malvern, Alabama, USA. Phylogenetic analysis based on the 16S rRNA gene placed the strain within the genus Pigmentiphaga with highest 16S rRNA gene sequence similarity of 98.74 % and 98....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003355

    authors: Kämpfer P,Busse HJ,Criscuolo A,Bizet C,Clermont D,McInroy JA,Glaeser SP

    更新日期:2019-06-01 00:00:00

  • Cohaesibacter haloalkalitolerans sp. nov., isolated from a soda lake, and emended description of the genus Cohaesibacter.

    abstract::Two novel Gram-stain-negative, motile, catalase-negative and oxidase-positive strains of bacteria (JC131(T) and JC112) were isolated from Lonar, a soda lake in India. Based on 16S rRNA gene sequence similarity studies, strains JC131(T) and JC112 belong to the family Cohaesibacteraceae of the class Alphaproteobacteria ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.050112-0

    authors: Sultanpuram VR,Lodha TD,Chintalapati VR,Chintalapati S

    更新日期:2013-11-01 00:00:00

  • Microbulbifer halophilus sp. nov., a moderately halophilic bacterium from north-west China.

    abstract::A Gram-negative, rod-shaped, moderately halophilic bacterium, designated strain YIM 91118(T), motile with a single polar flagellum, was isolated from Xinjiang province in north-west China and subjected to a polyphasic taxonomic study. Strain YIM 91118(T) grew optimally at 37 degrees C, pH 7.0-8.0 and 10% NaCl (w/v). I...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65519-0

    authors: Tang SK,Wang Y,Cai M,Lou K,Mao PH,Jin X,Jiang CL,Xu LH,Li WJ

    更新日期:2008-09-01 00:00:00

  • Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China.

    abstract::The taxonomic position of an actinomycete isolated from a lead-polluted soil in Gansu province, north-west China, was determined by using a polyphasic approach. Chemical and morphological properties of the isolate, designated strain CCNWHX 13-160(T), were similar to those of streptomycetes. Analysis of the almost comp...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.004713-0

    authors: Guo JK,Lin YB,Zhao ML,Sun R,Wang TT,Tang M,Wei GH

    更新日期:2009-06-01 00:00:00

  • Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples.

    abstract::Two strains of pink-coloured bacteria, 5516T-9(T) and 5516T-11(T), were isolated from an air sample collected in Korea. The taxonomic status of these novel strains was investigated by means of a polyphasic approach. The novel strains were Gram-positive, aerobic, non-spore-forming and coccus-shaped bacteria. The DNA G+...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.016030-0

    authors: Yoo SH,Weon HY,Kim SJ,Kim YS,Kim BY,Kwon SW

    更新日期:2010-05-01 00:00:00

  • Naumovozyma baii sp. nov., an ascomycetous yeast species isolated from rotten wood in a tropical forest.

    abstract::Two strains isolated from rotten wood were included in the Saccharomyces group based on morphological characteristics. However, rRNA gene sequence analyses (including the 18S rRNA gene, 26S rRNA gene D1/D2 domain and internal transcribed spacer region) indicated that these two strains represent a novel species of Naum...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.044909-0

    authors: Liu WQ,Han PJ,Qiu JZ,Wang QM

    更新日期:2012-12-01 00:00:00

  • Streptomyces polygonati sp. nov., an endophytic actinomycete isolated from a root of Polygonatum odoratum (Mill.).

    abstract::A novel actinomycete, designated strain NEAU-G9T, was isolated from the root of Polygonatum odoratum (Mill.) collected from Harbin, Heilongjiang province, north China, and was characterized using a polyphasic approach. Key morphological and chemotaxonomic properties confirmed the affiliation of strain NEAU-G9T to the ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000906

    authors: Guo S,Liu C,Liu S,Guan X,Guo L,Jia F,Wang X,Xiang W

    更新日期:2016-03-01 00:00:00

  • Phylogenetic analysis identifies the 'megabacterium' of birds as a novel anamorphic ascomycetous yeast, Macrorhabdus ornithogaster gen. nov., sp. nov.

    abstract::An organism commonly referred to as 'megabacterium' colonizes the gastric isthmus of many species of birds. It is weakly gram-positive and periodic acid-Schiff-positive and stains with silver stains. Previous studies have shown that it has a nucleus and a cell wall similar to those seen in fungi. Calcofluor white M2R ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02514-0

    authors: Tomaszewski EK,Logan KS,Snowden KF,Kurtzman CP,Phalen DN

    更新日期:2003-07-01 00:00:00

  • DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA.

    abstract::The present study was aimed at reducing the time and labour used to perform DNA-DNA hybridizations for classification of bacteria at the species level. A micro-well-format DNA hybridization method was developed and validated. DNA extractions were performed by a small-scale method and DNA was sheared mechanically into ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-3-1095

    authors: Christensen H,Angen O,Mutters R,Olsen JE,Bisgaard M

    更新日期:2000-05-01 00:00:00

  • Candida sorbosivorans sp. nov., a new member of the genus Candida Berkhout.

    abstract::A yeast, strain NCYC 2938T, was isolated from contaminated industrial material. This material was involved in a cascade continuous process for oxidizing sorbitol (D-glucitol) to L-sorbose. The isolate is similar, although not identical, to Candida geochares and Candida magnoliae in its physiological characteristics. S...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-51-3-1215

    authors: James SA,Bond CJ,Roberts IN

    更新日期:2001-05-01 00:00:00

  • Thermaerobacter subterraneus sp. nov., a novel aerobic bacterium from the Great Artesian Basin of Australia, and emendation of the genus Thermaerobacter.

    abstract::A strictly aerobic, thermophilic, gram-positive, spore-producing, rod-shaped bacterium (2.0-10.0 x 0.3 microm), designated isolate C21T, was isolated from a sample collected from an open drain run-off channel of a bore in the Great Artesian Basin of Australia (New Lorne Bore, registered number 17263). Isolate C21T gre...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-3-795

    authors: Spanevello MD,Yamamoto H,Patel BKC

    更新日期:2002-05-01 00:00:00