Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients.


OBJECTIVE:Synovitis associated with osteoarthritis (OA) is directly responsible for several clinical symptoms and reflects OA's structural progression. This study sought to analyze the expression of proinflammatory mediations, including Interleukin (IL)-17 and IL-22, which play key roles in regulating inflammatory processes, in inflamed and non-inflamed areas of osteoarthritic synovium. METHODS:Synovium from knees of 32 OA patients were collected at surgery. Macroscopic evaluation of inflammation enabled inflamed and non-inflamed areas to be separated. Samples were incubated to obtain tissue-conditioned media. Quantitative mRNA expression of proinflammatory mediators was analyzed by RT-PCR and protein levels by ELISA and gelatin zymography. Immunohistochemistry and histology were performed. RESULTS:Inflamed synovium were characterized by increased leukocyte infiltration and a higher vessel-to-tissue area ratio than non-inflamed tissues. Macrophages, T and B lymphocytes, and some neutrophils were found only in the inflamed tissue, and only in the subintimal layer. Levels of proinflammatory cytokines and MMP-9 were significantly higher in tissue-conditioned media from inflamed than non-inflamed tissues. Inflamed areas were associated with higher expression of IL-17 and IL-22, both correlated with the combined release of IL-6, IL-23, and TGFβ1. CONCLUSION:Our results showed that inflammatory cytokines, including IL-17 and IL-22, are expressed at higher levels by inflamed OA synovium and suggest IL-22 involvement in OA pathophysiology. This study will help identify new therapeutic strategies for OA, especially the targeting of IL-22 to decrease inflammation.


Deligne C,Casulli S,Pigenet A,Bougault C,Campillo-Gimenez L,Nourissat G,Berenbaum F,Elbim C,Houard X




Has Abstract


2015-11-01 00:00:00














  • Degeneration alters the biomechanical properties and structural composition of lateral human menisci.

    abstract:OBJECTIVE:Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regar...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Warnecke D,Balko J,Haas J,Bieger R,Leucht F,Wolf N,Schild NB,Stein SEC,Seitz AM,Ignatius A,Reichel H,Mizaikoff B,Dürselen L

    更新日期:2020-11-01 00:00:00

  • Fibromodulin-null murine knee joints display increased incidences of osteoarthritis and alterations in tissue biochemistry.

    abstract:OBJECTIVE:To elucidate variations in tissue ultrastructure and incidence of pathology between fibromodulin (FM)-null mice and wild-type (WT) animals. DESIGN:FM-null and WT siblings from different age groups were compared. Serial sections were made through paraffin-embedded whole knees and investigated histologically. ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Gill MR,Oldberg A,Reinholt FP

    更新日期:2002-10-01 00:00:00

  • Comparative pain reduction of oral non-steroidal anti-inflammatory drugs and opioids for knee osteoarthritis: systematic analytic review.

    abstract:OBJECTIVE:Summarize the comparative effectiveness of oral non-steroidal anti-inflammatory drugs (NSAIDs) and opioids in reducing knee osteoarthritis (OA) pain. METHODS:Two reviewers independently screened reports of randomized controlled trials (RCTs), published in English between 1982 and 2015, evaluating oral NSAIDs...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Smith SR,Deshpande BR,Collins JE,Katz JN,Losina E

    更新日期:2016-06-01 00:00:00

  • Mechanical symptoms as an indication for knee arthroscopy in patients with degenerative meniscus tear: a prospective cohort study.

    abstract:OBJECTIVE:According to prevailing consensus, patients with mechanical symptoms are those considered to most likely benefit from arthroscopic surgery. The aim of this study was to determine the value of using patients' pre-operative self-reports of mechanical symptoms as a justification surgery in patients with degenera...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Sihvonen R,Englund M,Turkiewicz A,Järvinen TL

    更新日期:2016-08-01 00:00:00

  • Comparison of measurement properties of the P4 pain scale and disease specific pain measures in patients with knee osteoarthritis.

    abstract:OBJECTIVE:To compare measurement properties of the P4 pain scale, Western Ontario and McMaster Universities Arthritis Index pain subscale (WOMAC-pain), and Intermittent and Constant Osteoarthritis Pain (ICOAP) measure in patients with knee osteoarthritis (OA). DESIGN:A secondary analysis from a randomized controlled t...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Robbins SM,Rastogi R,Howard J,Rosedale R

    更新日期:2014-06-01 00:00:00

  • Electroporation-mediated gene transfer of SOX trio to enhance chondrogenesis in adipose stem cells.

    abstract:OBJECTIVE:The aim of the present study was to determine if the electroporation-mediated gene transfer of SOX trio enhances the chondrogenic potential of adipose stem cells (ASCs). DESIGN:ASCs were transfected with SOX trio genes using an electroporation technique and cultured for 3 weeks. The pellets were analyzed for...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Im GI,Kim HJ

    更新日期:2011-04-01 00:00:00

  • Altered cartilage mechanics and histology in knee osteoarthritis: relation to clinical assessment (ICRS Grade).

    abstract:OBJECTIVE:Substantial changes in articular cartilage composition and mechanical properties occur during the development of osteoarthritis (OA). While softening in the initial stage is reported and sometimes used as an indicator of early OA, there is a lack of data relating the macroscopic appearance of cartilage to its...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kleemann RU,Krocker D,Cedraro A,Tuischer J,Duda GN

    更新日期:2005-11-01 00:00:00

  • Identification and pharmacological characterization of a novel inhibitor of autotaxin in rodent models of joint pain.

    abstract:OBJECTIVE:Autotaxin is a secreted lysophospholipase that mediates the conversion of lysophosphatidyl choline (LPC) to lysophosphatidic acid (LPA), a bioactive lipid mediator. Autotaxin levels in plasma and synovial fluid correlate with disease severity in patients with knee osteoarthritis (OA). The goal of this study w...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Thirunavukkarasu K,Swearingen CA,Oskins JL,Lin C,Bui HH,Jones SB,Pfeifer LA,Norman BH,Mitchell PG,Chambers MG

    更新日期:2017-06-01 00:00:00

  • Bone mineral distribution of the proximal tibia in gonarthrosis assessed in vivo by photon absorption.

    abstract::Regional bone mineral density (BMD, g/cm2) of the proximal tibia including the subchondral plate was measured in vivo by dual photon absorptiometry (DPA). Twenty-one women and six men with gonarthrosis (GA) were examined. Twenty-four healthy women served as controls for the women. The precision error of the BMD measur...

    journal_title:Osteoarthritis and cartilage

    pub_type: 临床试验,杂志文章


    authors: Madsen OR,Schaadt O,Bliddal H,Egsmose C,Sylvest J

    更新日期:1994-06-01 00:00:00

  • Osteoarthritis year 2012 in review: biomarkers.

    abstract:PURPOSE:Biomarkers provide useful diagnostic information by detecting cartilage degradation in osteoarthritis (OA), reflecting disease-relevant biological activity and predicting the course of disease progression. They also serve as surrogate endpoints in the drug discovery process. The aim of this narrative review was...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Mobasheri A

    更新日期:2012-12-01 00:00:00

  • Knee osteoarthritis patients with severe nocturnal pain have altered proximal tibial subchondral bone mineral density.

    abstract:OBJECTIVE:Our objective was to investigate relationships between proximal tibial subchondral bone mineral density (BMD) and nocturnal pain in patients with knee osteoarthritis (OA). METHODS:The preoperative knee of 42 patients booked for knee arthroplasty was scanned using quantitative computed tomography (QCT). Pain ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Burnett WD,Kontulainen SA,McLennan CE,Hazel D,Talmo C,Hunter DJ,Wilson DR,Johnston JD

    更新日期:2015-09-01 00:00:00

  • Cell and matrix morphology in articular cartilage from adult human knee and ankle joints suggests depth-associated adaptations to biomechanical and anatomical roles.

    abstract:OBJECTIVE:Marked differences exist between human knee and ankle joints regarding risks and progression of osteoarthritis (OA). Pathomechanisms of degenerative joint disease may therefore differ in these joints, due to differences in tissue structure and function. Focusing on structural issues, which are design goals fo...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Quinn TM,Häuselmann HJ,Shintani N,Hunziker EB

    更新日期:2013-12-01 00:00:00

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    abstract:OBJECTIVE:To employ a magnetic resonance (MR) imaging technique for quantitative assessment of thin cartilage layers, and to validate the cartilage volume and thickness measurements. METHODS:We investigated 10 normal elbow joints (age 20 to 69 years) with a 3D gradient echo sequence with selective water excitation (TR...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Graichen H,Springer V,Flaman T,Stammberger T,Glaser C,Englmeier KH,Reiser M,Eckstein F

    更新日期:2000-03-01 00:00:00

  • Effects of footwear on the knee adduction moment in medial knee osteoarthritis: classification criteria for flat flexible vs stable supportive shoes.

    abstract:OBJECTIVE:To validate simple criteria that distinguish flat flexible from stable supportive walking shoes by comparing their effects on the knee adduction moment (KAM) in people with medial knee osteoarthritis (OA). DESIGN:This was a cross-sectional biomechanical study. We proposed five criteria to differentiate flat ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Paterson KL,Bennell KL,Wrigley TV,Metcalf BR,Kasza J,Hinman RS

    更新日期:2017-02-01 00:00:00

  • REMOVED: Generation of cartilage microtissues by hanging drops: effect of different culture conditions.

    abstract::This article has been removed, consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Martinez I

    更新日期:2007-04-27 00:00:00

  • Novel biomarkers of intervertebral disc cells and evidence of stem cells in the intervertebral disc.

    abstract:OBJECTIVE:Rat intervertebral disc (IVD) is one of the most commonly used and cost-effective alternative models for human IVD. Many IVD related clinical studies need to be pre-tested on rat IVDs. However, studies on the heterogeneous cell clusters of the rat IVD are inadequate, and a further understanding of the marker ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Wang J,Huang Y,Huang L,Shi K,Wang J,Zhu C,Li L,Zhang L,Feng G,Liu L,Song Y

    更新日期:2020-12-15 00:00:00

  • Surgical reconstruction of ruptured anterior cruciate ligament prolongs trauma-induced increase of inflammatory cytokines in synovial fluid: an exploratory analysis in the KANON trial.

    abstract:OBJECTIVE:Prospectively monitor how treatment of acutely ruptured anterior cruciate ligament (ACL) affects biomarkers of inflammation and proteolytic degradation over 5 years. DESIGN:We studied 119 subjects with acute ACL injury from the randomized controlled knee anterior cruciate ligament, non-surgical versus surgic...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Larsson S,Struglics A,Lohmander LS,Frobell R

    更新日期:2017-09-01 00:00:00

  • Controlling joint instability after anterior cruciate ligament transection inhibits transforming growth factor-beta-mediated osteophyte formation.

    abstract:OBJECTIVE:Abnormal joint instability contributes to cartilage damage and osteophyte formation. We investigated whether controlling joint instability inhibited chronic synovial membrane inflammation and delayed osteophyte formation and examined the role of transforming growth factor-beta (TGF-β) signaling in the associa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Murata K,Kokubun T,Onitsuka K,Oka Y,Kano T,Morishita Y,Ozone K,Kuwabara N,Nishimoto J,Isho T,Takayanagi K,Kanemura N

    更新日期:2019-08-01 00:00:00

  • Engineering of hyaline cartilage with a calcified zone using bone marrow stromal cells.

    abstract:OBJECTIVE:In healthy joints, a zone of calcified cartilage (ZCC) provides the mechanical integration between articular cartilage and subchondral bone. Recapitulation of this architectural feature should serve to resist the constant shear force from the movement of the joint and prevent the delamination of tissue-engine...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Lee WD,Hurtig MB,Pilliar RM,Stanford WL,Kandel RA

    更新日期:2015-08-01 00:00:00

  • External knee adduction and flexion moments during gait and medial tibiofemoral disease progression in knee osteoarthritis.

    abstract:OBJECTIVE:Test the hypothesis that greater baseline peak external knee adduction moment (KAM), KAM impulse, and peak external knee flexion moment (KFM) during the stance phase of gait are associated with baseline-to-2-year medial tibiofemoral cartilage damage and bone marrow lesion progression, and cartilage thickness ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Chang AH,Moisio KC,Chmiel JS,Eckstein F,Guermazi A,Prasad PV,Zhang Y,Almagor O,Belisle L,Hayes K,Sharma L

    更新日期:2015-07-01 00:00:00

  • Anti-inflammatory activity of chondroitin sulfate.

    abstract::Osteoarthritis is primarily characterized by areas of destruction of articular cartilage and by synovitis. Articular damage and synovitis are secondary to local increase of pro-inflammatory cytokines (interleukin-1beta and tumor necrosis factor-alpha), enzymes with proteolytic activity (matrix metalloproteinases), and...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Iovu M,Dumais G,du Souich P

    更新日期:2008-01-01 00:00:00

  • Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice.

    abstract:OBJECTIVE:To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. METHODS:Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 day...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Zhu M,Chen M,Lichtler AC,O'Keefe RJ,Chen D

    更新日期:2008-01-01 00:00:00

  • Biomechanical and biochemical characteristics of the mandibular condylar cartilage.

    abstract::The human masticatory system consists of a mandible which is able to move with respect to the skull at its bilateral temporomandibular joint (TMJ) through contractions of the masticatory muscles. Like other synovial joints, the TMJ is loaded mechanically during function. The articular surface of the mandibular condyle...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Kuroda S,Tanimoto K,Izawa T,Fujihara S,Koolstra JH,Tanaka E

    更新日期:2009-11-01 00:00:00

  • Predictors of moderate-severe functional limitation after primary Total Knee Arthroplasty (TKA): 4701 TKAs at 2-years and 2935 TKAs at 5-years.

    abstract:OBJECTIVE:Investigate whether body mass index (BMI), comorbidity, gender and age predict patient-reported functional limitation 2- and 5-years after primary Total Knee Arthroplasty (TKA). METHODS:Overall moderate-severe activity limitation was defined as > or = 2 activities (walking, stairs, rising from chair) with mo...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Singh JA,O'Byrne M,Harmsen S,Lewallen D

    更新日期:2010-04-01 00:00:00

  • Differential response of porcine osteoblasts and chondrocytes in cell or tissue culture after 5-aminolevulinic acid-based photodynamic therapy.

    abstract:OBJECTIVE:Outcome in osteochondral allografting is limited by the immunological incompatibility of the grafted tissue. Based on a resistance of chondrocytes to photodynamic therapy in cell culture it is proposed that 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) might be used to inactivate bone while mai...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Bastian JD,Egli RJ,Ganz R,Hofstetter W,Leunig M

    更新日期:2009-04-01 00:00:00

  • Clinical, biochemical and imaging methods of assessing osteoarthritis and clinical trials with agents claiming 'chondromodulating' activity.

    abstract::This paper reviews, in a first part, methods used for the clinical assessment of osteoarthritis (OA) with special reference, in a second part, to trials of drugs claimed to be chondromodulating agents. The agents examined include glycosaminoglycan-peptide complex (GP-C, Rumalon) and glycosaminoglycan-polysulfate (GAGP...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Theiler R,Ghosh P,Brooks P

    更新日期:1994-03-01 00:00:00

  • Tumor risk by tissue engineering: cartilaginous differentiation of mesenchymal stem cells reduces tumor growth.

    abstract:OBJECTIVE:Implantation of autologous chondrocytes (AC) is a promising option for the treatment of cartilage defects, but problems with cell harvesting, dedifferentiation, or the donor age limit the clinical outcome. Mesenchymal stem cells (MSC) gain much interest because of their simple isolation and multipotential dif...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Akay I,Oxmann D,Helfenstein A,Mentlein R,Schünke M,Hassenpflug J,Kurz B

    更新日期:2010-03-01 00:00:00

  • Inhibition of LRRK2 restores parkin-mediated mitophagy and attenuates intervertebral disc degeneration.

    abstract:OBJECTIVE:To elucidate the role of LRRK2 in intervertebral disc degeneration (IDD) as well as its mitophagy regulation mechanism. METHODS:The expression of LRRK2 in human degenerative nucleus pulposus tissues as well as in oxidative stress-induced rat nucleus pulposus cells (NPCs) was detected by western blot. LRRK2 w...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Lin J,Zheng X,Zhang Z,Zhuge J,Shao Z,Huang C,Jin J,Chen X,Chen Y,Wu Y,Tian N,Sun L,Gao W,Zhou Y,Wang X,Zhang X

    更新日期:2021-01-09 00:00:00

  • Change in serum measurements of cartilage oligomeric matrix protein and association with the development and worsening of radiographic hip osteoarthritis.

    abstract:OBJECTIVE:To determine the association between changes in serum levels of cartilage oligomeric matrix protein (COMP) and serum N-telopeptide crosslinks (NTX) over a 6-year interval with the development and progression of radiographically apparent hip osteoarthritis (RHOA) in a community sample of elderly women over 8.3...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究


    authors: Chaganti RK,Kelman A,Lui L,Yao W,Javaid MK,Bauer D,Nevitt M,Lane NE,Study Of Osteoporotic Fractures Research Group Sof.

    更新日期:2008-05-01 00:00:00

  • Psychometric properties of the French translation of the reduced KOOS and HOOS (KOOS-PS and HOOS-PS).

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    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ornetti P,Perruccio AV,Roos EM,Lohmander LS,Davis AM,Maillefert JF

    更新日期:2009-12-01 00:00:00