Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients.


OBJECTIVE:Synovitis associated with osteoarthritis (OA) is directly responsible for several clinical symptoms and reflects OA's structural progression. This study sought to analyze the expression of proinflammatory mediations, including Interleukin (IL)-17 and IL-22, which play key roles in regulating inflammatory processes, in inflamed and non-inflamed areas of osteoarthritic synovium. METHODS:Synovium from knees of 32 OA patients were collected at surgery. Macroscopic evaluation of inflammation enabled inflamed and non-inflamed areas to be separated. Samples were incubated to obtain tissue-conditioned media. Quantitative mRNA expression of proinflammatory mediators was analyzed by RT-PCR and protein levels by ELISA and gelatin zymography. Immunohistochemistry and histology were performed. RESULTS:Inflamed synovium were characterized by increased leukocyte infiltration and a higher vessel-to-tissue area ratio than non-inflamed tissues. Macrophages, T and B lymphocytes, and some neutrophils were found only in the inflamed tissue, and only in the subintimal layer. Levels of proinflammatory cytokines and MMP-9 were significantly higher in tissue-conditioned media from inflamed than non-inflamed tissues. Inflamed areas were associated with higher expression of IL-17 and IL-22, both correlated with the combined release of IL-6, IL-23, and TGFβ1. CONCLUSION:Our results showed that inflammatory cytokines, including IL-17 and IL-22, are expressed at higher levels by inflamed OA synovium and suggest IL-22 involvement in OA pathophysiology. This study will help identify new therapeutic strategies for OA, especially the targeting of IL-22 to decrease inflammation.


Deligne C,Casulli S,Pigenet A,Bougault C,Campillo-Gimenez L,Nourissat G,Berenbaum F,Elbim C,Houard X




Has Abstract


2015-11-01 00:00:00














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    abstract:OBJECTIVE:To evaluate the thickness of cartilage at the posterior aspect of the medial and lateral condyle in Osteoarthritis (OA) knees compared to non-OA knees using computed tomography arthrography (CTA). DESIGN:535 consecutive knee CTAs (mean patient age = 48.7 ± 16.0; 286 males), were retrospectively analyzed. Kne...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Omoumi P,Michoux N,Roemer FW,Thienpont E,Vande Berg BC

    更新日期:2015-02-01 00:00:00

  • Analysis of changes in proteoglycan content in murine articular cartilage using image analysis.

    abstract::The extracellular matrix of articular cartilage consists mainly of type II collagen and large aggregating proteoglycan (aggrecan). During arthritis and other joint diseases, the proteoglycan (PG) level of cartilage matrix is diminished, leading to impairment of normal joint function. A new method is described for meas...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: van der Kraan PM,de Lange J,Vitters EL,van Beuningen HM,van Osch GJ,van Lent PL,van den Berg WB

    更新日期:1994-09-01 00:00:00

  • Stimulating effect of diacerein on TGF-beta1 and beta2 expression in articular chondrocytes cultured with and without interleukin-1.

    abstract:OBJECTIVE:Diacetylrhein or diacerein has shown efficacy in the treatment of both major forms of osteoarthritis (OA), coxarthrosis as well as gonarthrosis, improving clinical symptoms of the disease (pain reduction and algo-functional index). Both in-vitro and animal models studies suggest that diacerein may have also d...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Felisaz N,Boumediene K,Ghayor C,Herrouin JF,Bogdanowicz P,Galerra P,Pujol JP

    更新日期:1999-05-01 00:00:00

  • A randomized trial of patellofemoral bracing for treatment of patellofemoral osteoarthritis.

    abstract:PURPOSE:The number of effective knee osteoarthritis (OA) interventions, especially those tailored to specific compartmental involvement, are small. The objective of this study was to determine the efficacy of a realigning patellofemoral (PF) brace in improving pain and function among persons with symptomatic lateral PF...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Hunter DJ,Harvey W,Gross KD,Felson D,McCree P,Li L,Hirko K,Zhang B,Bennell K

    更新日期:2011-07-01 00:00:00

  • Joint-dependent response to impact and implications for post-traumatic osteoarthritis.

    abstract:OBJECTIVE:The prevalence of osteoarthritis (OA) varies between joints. Cartilage in eight different joints was evaluated to elucidate the disparate susceptibilities between joints to post-traumatic OA (PTOA) and provide evidence for joint-specific clinical treatments. The hypothesis was that cartilage in different join...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Novakofski KD,Berg LC,Bronzini I,Bonnevie ED,Poland SG,Bonassar LJ,Fortier LA

    更新日期:2015-07-01 00:00:00

  • Development and validation of a new population-based simulation model of osteoarthritis in New Zealand.

    abstract:OBJECTIVE:To describe the construction and preliminary validation of a new population-based microsimulation model developed to analyse the health and economic burden and cost-effectiveness of treatments for knee osteoarthritis (OA) in New Zealand (NZ). METHOD:We developed the New Zealand Management of Osteoarthritis (...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Wilson R,Abbott JH

    更新日期:2018-04-01 00:00:00

  • Tibial cancellous bone changes in patients with knee osteoarthritis. A short-term longitudinal study using Fractal Signature Analysis.

    abstract:OBJECTIVE:To quantify tibial cancellous bone changes over 24 months in patients with medial compartment knee osteoarthritis (OA) subdivided into those with slow or detectable joint space narrowing (JSN). METHOD:Digitised macroradiographs (4x) were obtained at baseline and 24 months from 40 patients (F:26) and subdivid...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Messent EA,Ward RJ,Tonkin CJ,Buckland-Wright C

    更新日期:2005-06-01 00:00:00

  • Varus thrust during walking and the risk of incident and worsening medial tibiofemoral MRI lesions: the Multicenter Osteoarthritis Study.

    abstract:OBJECTIVE:To determine the association of varus thrust during walking to incident and worsening medial tibiofemoral cartilage damage and bone marrow lesions (BMLs) over 2 years in older adults with or at risk for osteoarthritis (OA). METHOD:Subjects from the Multicenter Osteoarthritis Study (MOST) were studied. Varus ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究


    authors: Wink AE,Gross KD,Brown CA,Guermazi A,Roemer F,Niu J,Torner J,Lewis CE,Nevitt MC,Tolstykh I,Sharma L,Felson DT

    更新日期:2017-06-01 00:00:00

  • Osteophyte development--molecular characterization of differentiation stages.

    abstract:OBJECTIVE:Osteophytes are non-neoplastic osteo-cartilaginous protrusions growing at the margins of osteoarthritic joints. They can not only be considered as in situ repair tissue, but also represent an excellent in vivo model for induced cartilage repair processes. Our focus was to identify different steps of osteophyt...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Gelse K,Söder S,Eger W,Diemtar T,Aigner T

    更新日期:2003-02-01 00:00:00

  • Effects of treadmill running and limb immobilization on knee cartilage degeneration and locomotor joint kinematics in rats following knee meniscal transection.

    abstract:OBJECTIVE:This study examined the effects of reduced and elevated weight bearing on post-traumatic osteoarthritis (PTOA) development, locomotor joint kinematics, and degree of voluntary activity in rats following medial meniscal transection (MMT). DESIGN:Twenty-one adult rats were subjected to MMT surgery of the left ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Tsai LC,Cooper ES,Hetzendorfer KM,Warren GL,Chang YH,Willett NJ

    更新日期:2019-12-01 00:00:00

  • Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection.

    abstract:OBJECTIVE:Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship betwe...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Chou MC,Tsai PH,Huang GS,Lee HS,Lee CH,Lin MH,Lin CY,Chung HW

    更新日期:2009-04-01 00:00:00

  • Validity of self-report measures of pain and disability for persons who have undergone arthroplasty for osteoarthritis of the carpometacarpal joint of the hand.

    abstract:OBJECTIVE:To establish the validity of three self-report scales used to measure function following arthroplasty for osteoarthritis (OA) of the carpometacarpal joint. METHOD:Persons with OA of the carpometacarpal joint (n=122) were assessed on one occasion 9-117 months following tendon interposition arthroplasty. They ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: MacDermid JC,Wessel J,Humphrey R,Ross D,Roth JH

    更新日期:2007-05-01 00:00:00

  • Cell and matrix morphology in articular cartilage from adult human knee and ankle joints suggests depth-associated adaptations to biomechanical and anatomical roles.

    abstract:OBJECTIVE:Marked differences exist between human knee and ankle joints regarding risks and progression of osteoarthritis (OA). Pathomechanisms of degenerative joint disease may therefore differ in these joints, due to differences in tissue structure and function. Focusing on structural issues, which are design goals fo...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Quinn TM,Häuselmann HJ,Shintani N,Hunziker EB

    更新日期:2013-12-01 00:00:00

  • Abundant LacZ activity in the absence of Cre expression in the normal and inflamed synovium of adult Col2a1-Cre; ROSA26RLacZ reporter mice.

    abstract::Recent analyses of Col2a1-Cre; ROSA26R reporter mice showed that synovial fibroblasts in 7-day mice were LacZ positive, due to a history of Col2a1-Cre expression conferred by their origin in the interzone of the developing joint. We have examined LacZ staining in adult Col2a1-Cre(+/0); ROSA26R(LacZ) mice, with and wit...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fosang AJ,Golub SB,East CJ,Rogerson FM

    更新日期:2013-02-01 00:00:00

  • Cartilage degeneration in different human joints.

    abstract::Variations among joints in the initiation and progression of degeneration may be explained, in part, by metabolic, biochemical and biomechanical differences. Compared to the cartilage in the knee joint, ankle cartilage has a higher content of proteoglycans and water, as well as an increased rate of proteoglycan turnov...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Kuettner KE,Cole AA

    更新日期:2005-02-01 00:00:00

  • The surgical destabilization of the medial meniscus (DMM) model of osteoarthritis in the 129/SvEv mouse.

    abstract:OBJECTIVE:To evaluate anterior cruciate ligament transection (ACLT) and destabilization of the medial meniscus (DMM) surgical instability models of osteoarthritis (OA) in the 129/SvEv mouse knee joint. DESIGN:Micro-surgical techniques were used to perform ACLT or DMM under direct visualization. Histological scoring wa...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Glasson SS,Blanchet TJ,Morris EA

    更新日期:2007-09-01 00:00:00

  • Detection of degenerative cartilage disease: comparison of high-resolution morphological MR and quantitative T2 mapping at 3.0 Tesla.

    abstract:OBJECTIVE:The aim of the study was to investigate the association of T2 relaxation times of the knee with early degenerative cartilage changes. Furthermore the impact of unloading the knee on T2 values was evaluated. METHODS:Forty-three patients with knee pain and an ICRS (International Cartilage Repair Society) carti...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Apprich S,Welsch GH,Mamisch TC,Szomolanyi P,Mayerhoefer M,Pinker K,Trattnig S

    更新日期:2010-09-01 00:00:00

  • Chondroitin sulfate and/or glucosamine hydrochloride for Kashin-Beck disease: a cluster-randomized, placebo-controlled study.

    abstract:OBJECTIVE:To evaluate the efficacy and safety of chondroitin sulfate and/or glucosamine hydrochloride in alleviating symptoms and improving the dysfunction of Kashin-Beck disease (KBD) patients. METHODS:We undertook a cluster-randomized, placebo-controlled trial in 251 patients with KBD. Participants were randomly all...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Yue J,Yang M,Yi S,Dong B,Li W,Yang Z,Lu J,Zhang R,Yong J

    更新日期:2012-07-01 00:00:00

  • Joint inflammation increases glucosamine levels attained in synovial fluid following oral administration of glucosamine hydrochloride.

    abstract:OBJECTIVE:To compare synovial glucosamine levels in normal and inflamed equine joints following oral glucosamine administration and to determine whether single dose administration alters standard synovial parameters of inflammation. METHODS:Eight adult horses were studied. On weeks 1 and 2, all horses received 20mg/kg...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Meulyzer M,Vachon P,Beaudry F,Vinardell T,Richard H,Beauchamp G,Laverty S

    更新日期:2009-02-01 00:00:00

  • Hypoxia, RONS and energy metabolism in articular cartilage.

    abstract:OBJECTIVE:Increased pro-inflammatory cytokines and reactive oxygen and nitrogen species (RONS) occur in osteoarthritis (OA). Oxygen tension can alter the levels of RONS induced by interleukin-1 (IL-1). RONS such as nitric oxide (NO) can alter energy metabolism. The aim of this study was to determine if oxygen tension a...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fermor B,Gurumurthy A,Diekman BO

    更新日期:2010-09-01 00:00:00

  • Pain, motor and gait assessment of murine osteoarthritis in a cruciate ligament transection model.

    abstract:OBJECTIVE:The major complaint of Osteoarthritis (OA) patients is pain. However, due to the nature of clinical studies and the limitation of animal studies, few studies have linked function impairment and behavioral changes in OA animal models to cartilage loss and histopathology. Our objective was to study surrogate ma...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ruan MZ,Patel RM,Dawson BC,Jiang MM,Lee BH

    更新日期:2013-09-01 00:00:00

  • Dynamic knee loading is related to cartilage defects and tibial plateau bone area in medial knee osteoarthritis.

    abstract:OBJECTIVE:To evaluate the relationship between dynamic mechanical loading, as indicated by external knee adduction moment (KAM) measures during walking, and measures of articular cartilage morphology and subchondral bone size in people with medial knee osteoarthritis (OA). DESIGN:180 individuals with radiographic medi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Creaby MW,Wang Y,Bennell KL,Hinman RS,Metcalf BR,Bowles KA,Cicuttini FM

    更新日期:2010-11-01 00:00:00

  • Characterization of infiltrating T cells and Th1/Th2-type cytokines in the synovium of patients with osteoarthritis.

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    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Ishii H,Tanaka H,Katoh K,Nakamura H,Nagashima M,Yoshino S

    更新日期:2002-04-01 00:00:00

  • Safety, tolerability, and pharmacodynamics of an anti-interleukin-1α/β dual variable domain immunoglobulin in patients with osteoarthritis of the knee: a randomized phase 1 study.

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    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Wang SX,Abramson SB,Attur M,Karsdal MA,Preston RA,Lozada CJ,Kosloski MP,Hong F,Jiang P,Saltarelli MJ,Hendrickson BA,Medema JK

    更新日期:2017-12-01 00:00:00

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    abstract:OBJECTIVE:The effects of mechanical deformation of intact cartilage tissue on chondrocyte biosynthesis in situ have been well documented, but the mechanotransduction pathways that regulate such phenomena have not been elucidated completely. The goal of this study was to examine the effects of tissue deformation on the ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Szafranski JD,Grodzinsky AJ,Burger E,Gaschen V,Hung HH,Hunziker EB

    更新日期:2004-12-01 00:00:00

  • Upregulation of lipocalin-2 (LCN2) in osteoarthritic cartilage is not necessary for cartilage destruction in mice.

    abstract:OBJECTIVE:Lipocalin-2 (LCN2) is a recently characterized adipokine that is upregulated in chondrocytes treated with pro-inflammatory mediators and in the synovial fluid of osteoarthritis (OA) patients. Here, we explored the in vivo functions of LCN2 in OA cartilage destruction in mice. METHODS:The expression levels of...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Choi WS,Chun JS

    更新日期:2017-03-01 00:00:00

  • T1rho relaxation time of the meniscus and its relationship with T1rho of adjacent cartilage in knees with acute ACL injuries at 3 T.

    abstract:OBJECTIVE:The aim of this study was to assess the reproducibility of meniscus T1rho measurements, and to study T1rho relaxation time in the lateral meniscus (LM) and its relationship with adjacent cartilage T1rho in knees with acute anterior cruciate ligament (ACL) injuries at 3T magnetic resonance imaging. METHOD:Qua...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Bolbos RI,Link TM,Ma CB,Majumdar S,Li X

    更新日期:2009-01-01 00:00:00

  • Long-term effectiveness of exercise therapy in patients with osteoarthritis of the hip or knee: a randomized controlled trial comparing two different physical therapy interventions.

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    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Pisters MF,Veenhof C,Schellevis FG,De Bakker DH,Dekker J

    更新日期:2010-08-01 00:00:00

  • Skin and urine pentosidine weakly correlate with joint damage in a cohort of patients with early signs of osteoarthritis (CHECK).

    abstract:OBJECTIVES:Age-related changes in articular cartilage are likely to play a role in the aetiology of osteoarthritis (OA). One of the major age-related changes in cartilage is the accumulation of advanced-glycation-endproducts (AGEs). Since, cartilage tissue is not readily available from patients for studying AGE levels,...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,多中心研究


    authors: Vos PA,DeGroot J,Huisman AM,Oostveen JC,Marijnissen AC,Bijlsma JW,van El B,Zuurmond AM,Lafeber FP

    更新日期:2010-10-01 00:00:00

  • Subregional statistical shape modelling identifies lesser trochanter size as a possible risk factor for radiographic hip osteoarthritis, a cross-sectional analysis from the Osteoporotic Fractures in Men Study.

    abstract:OBJECTIVE:Statistical shape modelling (SSM) of hip dual-energy X-ray absorptiometry (DXA) scans has identified relationships between hip shape and radiographic hip OA (rHOA). We aimed to further elucidate shape characteristics related to rHOA by focusing on subregions identified from whole-hip shape models. METHOD:SSM...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Faber BG,Bredbenner TL,Baird D,Gregory J,Saunders F,Giuraniuc CV,Aspden RM,Lane NE,Orwoll E,Tobias JH,Osteoporotic Fractures in Men (MrOS) Study Research Group.

    更新日期:2020-08-01 00:00:00