Astonishing advances in mouse genetic tools for biomedical research.


:The humble house mouse has long been a workhorse model system in biomedical research. The technology for introducing site-specific genome modifications led to Nobel Prizes for its pioneers and opened a new era of mouse genetics. However, this technology was very time-consuming and technically demanding. As a result, many investigators continued to employ easier genome manipulation methods, though resulting models can suffer from overlooked or underestimated consequences. Another breakthrough, invaluable for the molecular dissection of disease mechanisms, was the invention of high-throughput methods to measure the expression of a plethora of genes in parallel. However, the use of samples containing material from multiple cell types could obfuscate data, and thus interpretations. In this review we highlight some important issues in experimental approaches using mouse models for biomedical research. We then discuss recent technological advances in mouse genetics that are revolutionising human disease research. Mouse genomes are now easily manipulated at precise locations thanks to guided endonucleases, such as transcription activator-like effector nucleases (TALENs) or the CRISPR/Cas9 system, both also having the potential to turn the dream of human gene therapy into reality. Newly developed methods of cell type-specific isolation of transcriptomes from crude tissue homogenates, followed by detection with next generation sequencing (NGS), are vastly improving gene regulation studies. Taken together, these amazing tools simplify the creation of much more accurate mouse models of human disease, and enable the extraction of hitherto unobtainable data.


Swiss Med Wkly


Swiss medical weekly


Kaczmarczyk L,Jackson WS




Has Abstract


2015-10-29 00:00:00












  • Accuracy of doctors' anthropometric measurements in general practice.

    abstract:PURPOSE:There is increasing pressure on general practitioners (GPs) to identify patients with abdominal obesity in order to reduce the life-threatening consequences of this condition in the population. We aimed to confirm previous findings on the inaccuracy of anthropometric measurements performed by GPs in an academic...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Sebo P,Haller D,Pechère-Bertschi A,Bovier P,Herrmann F

    更新日期:2015-02-21 00:00:00

  • Cost of disorders of the brain in Switzerland with a focus on mental disorders.

    abstract:QUESTIONS UNDER STUDY:This study aims at estimating the costs of disorders of the brain in Switzerland based on the published epidemiological and economic evidence. METHODS:the data presented for Switzerland are derived from the "cost of disorders of the brain in Europe" study of the European Brain Counsil (EBC). Avai...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Jäger M,Sobocki P,Rössler W

    更新日期:2008-01-12 00:00:00

  • Left ventricular hypertrophy: diagnostics pitfalls.

    abstract::Isolated left ventricular hypertrophy, in the absence of hypertension or aortic stenosis, is commonly attributed to hypertrophic cardiomyopathy (HCM). According to the clinical setting, however, other differential diagnoses should be considered. The diagnosis of cardiac amyloidosis may be very challenging especially w...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Bonvini RF,Camenzind E

    更新日期:2004-05-15 00:00:00

  • Proteostasis: bad news and good news from the endoplasmic reticulum.

    abstract::The endoplasmic reticulum (ER) is an intracellular compartment dedicated to the synthesis and maturation of secretory and membrane proteins, totalling about 30% of the total eukaryotic cells proteome. The capacity to produce correctly folded polypeptides and to transport them to their correct intra- or extracellular d...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章,评审


    authors: Noack J,Brambilla Pisoni G,Molinari M

    更新日期:2014-08-21 00:00:00

  • Reactive hypoglycaemia due to late dumping syndrome: successful treatment with acarbose.

    abstract::Reactive hypoglycaemia is a rare disease which occurs postprandially in everyday life involving blood glucose levels below 2.5 to 2.8 mmol/l. We report on a 66-year-old patient who developed symptomatic reactive hypoglycaemia due to late dumping syndrome 10 years after oesophagectomy with cervical anastomosis. A 75 g ...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Imhof A,Schneemann M,Schaffner A,Brändle M

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  • Vaccination recommendations for adult patients with autoimmune inflammatory rheumatic diseases.

    abstract:BACKGROUND:The number of individuals with autoimmune inflammatory rheumatic diseases (AIIRDs) treated with immunosuppressive drugs is increasing steadily. The variety of immunosuppressive drugs and, in particular, biological therapies is also rising. The immunosuppressants, as well as the AIIRD itself, increase the ris...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Bühler S,Eperon G,Ribi C,Kyburz D,van Gompel F,Visser LG,Siegrist CA,Hatz C

    更新日期:2015-07-28 00:00:00

  • Is the Eichenholtz classification still valid for the diabetic Charcot foot?

    abstract::In his 1966 monograph "Charcot joints", Sidney N. Eichenholtz (1909-2000) described "three well defined stages … in the course and development of a Charcot joint", based on plain X-rays of 68 patients. Since then, medical imaging has advanced very much: computed tomography and magnetic resonance imaging (MRI) scans ex...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章,评审


    authors: Chantelau EA,Grützner G

    更新日期:2014-04-24 00:00:00

  • Recommendations for the treatment of hypertension in the elderly and very elderly--a scotoma within international guidelines.

    abstract::The recommendations of international scientific societies for the treatment of hypertension in the geriatric population are different. Lack of outcome trials, non-standardised terminology as well as differing levels of evidence contribute to the inconsistencies in the guidelines. This review article compares six inter...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章,评审


    authors: Schäfer HH,De Villiers JN,Sudano I,Dischinger S,Theus GR,Zilla P,Dieterle T

    更新日期:2012-05-09 00:00:00

  • Screening strategy for Chagas disease in a non-endemic country (Switzerland): a prospective evaluation.

    abstract::The WHO recommends screening of Latin American migrants for Chagas disease to reduce morbidity and mortality and increase the likelihood of eradicating the disease. The objective was to assess the feasibility and acceptability of a screening strategy in one Swiss canton. From February 2011 to September 2012, people at...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Da Costa-Demaurex C,Cárdenas MT,Aparicio H,Bodenmann P,Genton B,D'Acremont V

    更新日期:2019-04-04 00:00:00

  • Bosentan for patients with steroid-resistant pulmonary sarcoidosis: a randomised controlled trial.

    abstract:BACKGROUND:Sarcoidosis is a disorder of unknown aetiology. Most patients have steroid-responsive disease, but side effects and steroid resistance may necessitate alternative treatments. Endothelin has in-vitro fibrogenic activity and the endothelin system is activated in sarcoidosis. OBJECTIVES:We studied the efficacy...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章,随机对照试验


    authors: Hostettler K,Baty F,Kleiner R,Junker L,Tamm M,Brutsche M

    更新日期:2018-10-28 00:00:00

  • Variation in incidence of orthopaedic surgery between populations with basic or basic plus supplementary health insurance in Switzerland.

    abstract:QUESTIONS UNDER STUDY:The aim of the study was to analyse the effects of supplementary health insurance on the incidence of hospitalisations for musculoskeletal conditions in Switzerland. METHODS:Cross sectional and small area analyses of surgical interventions for major musculoskeletal disorders in Switzerland were c...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Busato A,Widmer M,Matter P

    更新日期:2011-02-03 00:00:00

  • Additional malignancies in patients with neuroendocrine tumours: analysis of the SwissNET registry.

    abstract:PRINCIPLES:Neuroendocrine neoplasms (NENs) are believed to be associated with an increased risk for additional malignancies (AMs). We aimed to (1) assess the occurrence of AM in NEN patients (2) investigate the characteristics and temporal relationship of NEN patients with and without AM. METHODS:The SwissNET registry...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Kollár A,Blank A,Perren A,Bütikofer L,Stettler C,Christ E

    更新日期:2016-11-12 00:00:00

  • Loss of a preterm infant: psychological aspects in parents.

    abstract:QUESTIONS UNDER STUDY:The unexpected death of a preterm infant is an extremely painful situation for parents. Despite a number of quantitative studies, little is known about parents' inner experience. The aim of this study was to gather more in-depth information about what preoccupies parents in this situation of suffe...

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    pub_type: 杂志文章


    authors: Glaser A,Bucher HU,Moergeli H,Fauchère JC,Buechi S

    更新日期:2007-07-14 00:00:00

  • 31 years of lung cancer in the canton of Zurich, Switzerland: incidence trends by sex, histology and laterality.

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    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Oberli LS,Valeri F,Korol D,Rohrmann S,Dehler S

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  • Time trends of positivity rates from foodborne pathogen testing in Switzerland, 2003 to 2012.

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    pub_type: 杂志文章


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  • Long term effects of comprehensive cardiac rehabilitation in an inpatient and outpatient setting.

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    pub_type: 杂志文章


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  • Risk behaviours among native and immigrant youths in Switzerland: a cross-sectional study.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Vilpert S,Bolliger E,Borrat-Besson C,Borasio GD,Maurer J

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  • Arrhythmias in patients with surgically treated atrial septal defects.

    abstract::Atrial arrhythmias complicate the clinical course of adult patients with an atrial septal defect. Atrial flutter is more prevalent in younger patients, and frequently regresses after surgical defect closure. Atrial fibrillation however, which results from a chronic underlying disease, rarely reverts to sinus rhythm af...

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    pub_type: 杂志文章,评审


    authors: Berger F,Vogel M,Kretschmar O,Dave H,Prêtre R,Dodge-Khatami A

    更新日期:2005-03-19 00:00:00

  • Diagnostic competence of Swiss general practitioners in skin cancer.

    abstract:QUESTIONS UNDER STUDY:In Switzerland, skin cancer is one of the most prevalent neoplasms. General practitioners (GPs) are often faced with suspicious skin lesions in their patients. The aim of our study was to assess GPs' competence to diagnose skin cancer and to examine whether this can be improved by a one-day dermat...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Badertscher N,Braun RP,Held U,Kofmehl R,Senn O,Hofbauer GF,Rossi PO,Wensing M,Rosemann T,Tandjung R

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  • Low-molecular-weight heparin in patients with renal insufficiency.

    abstract:BACKGROUND:Low-molecular-weight heparins (LMWH) have been shown to be safer, more effective and more convenient than unfractionated heparin (UFH) in many clinical situations. However, their use is limited in patients with renal insufficiency (RI) due to bioaccumulation. METHOD:The literature is critically reviewed and...

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    pub_type: 杂志文章,评审


    authors: Schmid P,Fischer AG,Wuillemin WA

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    journal_title:Swiss medical weekly

    pub_type: 临床试验,杂志文章


    authors: Roll A,Wüthrich B,Schmid-Grendelmeier P,Hofbauer G,Ballmer-Weber BK

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  • Improved results after lung transplantation--analysis of factors.

    abstract::Better recipient selection, sophisticated postoperative surveillance and new immunosuppressive and anti-infective regimens can improve the results of lung transplantation. We compared the results of lung transplants performed between 1992 and 1996 (early period; 47) and between 1997 and 2000 (recent period; 46) in a c...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Speich R,Boehler A,Zalunardo MP,Stocker R,Russi EW,Weder W

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  • Idiopathic pulmonary fibrosis--a disorder of alveolar wound repair?

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    journal_title:Swiss medical weekly

    pub_type: 杂志文章,评审


    authors: Geiser T

    更新日期:2003-07-26 00:00:00

  • Osteoporosis drug treatment: duration and management after discontinuation. A position statement from the SVGO/ASCO.

    abstract::Antiosteoporotic drugs are recommended in patients with fragility fractures and in patients considered to be at high fracture risk on the basis of clinical risk factors and/or low bone mineral density. As first-line treatment most patients are started with an antiresorptive treatment, i.e. drugs that inhibit osteoclas...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Meier C,Uebelhart B,Aubry-Rozier B,Birkhäuser M,Bischoff-Ferrari HA,Frey D,Kressig RW,Lamy O,Lippuner K,Stute P,Suhm N,Ferrari S

    更新日期:2017-08-16 00:00:00

  • Device-less patent foramen ovale closure by radiofrequency thermal energy.

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    journal_title:Swiss medical weekly

    pub_type: 临床试验,杂志文章


    authors: Walpoth NB,Habermacher K,Moarof I,Watson S,Wahl A,Windecker S,Schönenberger C,Meier B

    更新日期:2008-02-23 00:00:00

  • Genetic and phenotypic determinants of blood pressure and other cardiovascular risk factors (GAPP).

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    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Conen D,Schön T,Aeschbacher S,Paré G,Frehner W,Risch M,Risch L

    更新日期:2013-01-08 00:00:00

  • Treatment outcomes of multidrug-resistant tuberculosis in Switzerland.

    abstract:OBJECTIVE:To assess outcomes 24 months after treatment start for multidrug-resistant tuberculosis (MDR-TB). METHODS:Cohort study of all culture-positive MDR-TB cases notified in Switzerland from 01/2003 to 07/2010. RESULTS:Fifty-one cases were observed, with a median age of 26 years (range 2-56). Twenty-seven were ma...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Helbling P,Altpeter E,Egger JM,Zellweger JP

    更新日期:2014-12-04 00:00:00

  • Antifungal chemotherapy: advances and perspectives.

    abstract::Invasive fungal infections have emerged as important causes of morbidity and mortality in immunocompromised patients. In response to this challenge, the field of antifungal chemotherapy has considerably expanded. Fluconazole and itraconazole, introduced in the late 1980s, were the first durably useful alternatives to ...

    journal_title:Swiss medical weekly

    pub_type: 杂志文章,评审


    authors: Groll AH,Walsh TJ

    更新日期:2002-06-15 00:00:00

  • Evidence for age-based rationing in a Swiss university hospital.

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    journal_title:Swiss medical weekly

    pub_type: 杂志文章


    authors: Jenni D,Osterwalder R,Osswald S,Buser P,Pfisterer M

    更新日期:2001-11-10 00:00:00